Hydrocarbon and Lipid Microbiology Protocols: Meso- And Microcosms, McGenity Terry J., Timmis Kenneth N., Nogales Balbina
Автор: Terry J. McGenity; Kenneth N. Timmis; Balbina Noga Название: Hydrocarbon and Lipid Microbiology Protocols ISBN: 3662504499 ISBN-13(EAN): 9783662504499 Издательство: Springer Рейтинг: Цена: 19564.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This Volume presents protocols for investigating the genetic, metabolic and ecological potential and functional analysis of microbial communities.
Автор: Terry J. McGenity; Kenneth N. Timmis; Balbina Noga Название: Hydrocarbon and Lipid Microbiology Protocols ISBN: 3662491389 ISBN-13(EAN): 9783662491386 Издательство: Springer Рейтинг: Цена: 18167.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
This Volume features protocols for investigating the hydrocarbon- and lipid-specific activities of microbes. They include methods for studying chemotaxis, the colonisation of hydrocarbon surfaces, hydrocarbon uptake, respiration, nitrogen fixation, sulphate reduction, membrane stabilisation through cis-trans isomerisation of membrane fatty acids, and the production of biosurfactants and biopolymers in response to the presence of hydrocarbons. A protocol for studying the ability of microbes to control the concentration of hydrocarbons in their aqueous environment is also described, and phenotyping methods to reveal microbes’ more general metabolic activities are presented. Several protocols for investigating acid production in connection with oil souring and biocorrosion by microbes in oil well, oil transportation and storage settings are presented. Lastly, protocols for measuring methanogenesis, as an example of microbial hydrocarbon production, are described.<
Hydrocarbon and Lipid Microbiology Protocols
There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.
Описание: Introduction to Microbial Quantitation, Community Profiling, and Array Approaches.- A cell extraction method for oily sediments.- Introduction to microplate MPN-enumeration of hydrocarbon degraders.- Primers for dsr genes and Most Probable Number Method for detection of sulfate-reducing bacteria in oil reservoirs.- Microbial biomass and community analysis by PLFA analysis.- Real-time PCR approaches for analysis of hydrocarbon-degrading bacterial communities.- Clone libraries of ribosomal RNA gene sequences for characterization of microbial communities.- Denaturing gradient gel electrophoresis (DGGE) for microbial community analysis.- Microbial community profiling: SSCP and T-RFLP techniques.- Microbial community analysis by single-amplicon high-throughput sequencing (metagenetics) - preparation, data handling and ecological analysis.- Multiplex fluorescent antibody microarrays and antibody graphs for microbial and biomarker detection in the environment.- Studying protistan communities in hydrocarbon-contaminated environments.
Автор: Terry J. McGenity; Kenneth N. Timmis; Balbina Noga Название: Hydrocarbon and Lipid Microbiology Protocols ISBN: 3662531100 ISBN-13(EAN): 9783662531105 Издательство: Springer Рейтинг: Цена: 18167.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This Volume presents applications of hydrocarbon microbiology in the context of environmental pollutant degradation, covering pollutants such as petroleum and related wastes (i.e.
Автор: McGenity Название: Hydrocarbon and Lipid Microbiology Protocols ISBN: 3662491354 ISBN-13(EAN): 9783662491355 Издательство: Springer Рейтинг: Цена: 18167.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This Volume provides protocols for the biochemical analysis of hydrocarbon- and lipid-relevant products, cell components and activities of microbes that interact with hydrophobic compounds. They include methods for the extraction, purification and characterisation of surface tension-reducing bioemulsifiers and biosurfactants that increase the surface area and hence bioavailability of hydrophobic substrates. Protocols for the isolation and biochemical analysis of lipids and polyhydroxyalkanoates, food storage products made during nutrient abundance that represent important biotechnological products, are presented. The extraction of membrane lipid rafts, sub-organelles that fulfil important functional roles for the cell membrane, and the isolation and characterisation of membrane phospholipid biomarkers, are also described. The purification and characterisation of integral membrane hydrocarbon-oxidising enzymes are addressed. Lastly, two generic methods for the genetic analysis of catabolic pathways and analysis of ligand binding are presented.
Hydrocarbon and Lipid Microbiology Protocols
There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.
Описание: Introduction: Single cell biotechnology for uncultured microorganisms.- Single-cell cultivation in microfluidic devices.- Single-cell bacterial bioreporter assays to measure hydrocarbons.- Whole genome amplification of single bacterial cells.- Detection of specific mRNA molecules in hydrocarbon-degrading bacteria.- Localisation of proteins within intact bacterial cells using fluorescent protein fusions.- Raman-FISH.- Single-cell metabolomics.- Marrying single molecules to single cells: Protocols for the study of the bacterial proto-ring components essential for division.- Single cell biotechnology for uncultured microorganisms.- Single cell and systems biology tools for biofuel production.- Detection of protein-synthesizing microorganisms in the environment via bioorthogonal non-canonical amino acid tagging (BONCAT).- Flow cytometric determination of microbial abundances and its use to obtain indices of community structure and relative activity.- Protocols for visualizing horizontal gene transfer in Gram-negative bacteria through natural competence.
Автор: McGenity Terry J., Timmis Kenneth N., Nogales Balbina Название: Hydrocarbon and Lipid Microbiology Protocols: Primers ISBN: 3662570572 ISBN-13(EAN): 9783662570579 Издательство: Springer Рейтинг: Цена: 13974.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Introduction.- Current landscape of biomolecular approaches for assessing biodegradation of aromatic hydrocarbons.- Primers - bacterial genes encoding enzymes for aerobic alkane degradation.- Primers - functional genes for anaerobic hydrocarbon degrading microbes.- Primers - functional marker genes for methylotrophs and methanotrophs.- Primers - functional genes and 16S rRNA genes for methanogens.- Primers - functional genes for aerobic chlorinated hydrocarbon degrading microbes.- Primers that target functional genes of organohalide-respiring bacteria.- Primers - functional genes for nitrogen-cycling microbes in oil reservoirs.
Автор: McGenity Название: Hydrocarbon and Lipid Microbiology Protocols ISBN: 3662504278 ISBN-13(EAN): 9783662504277 Издательство: Springer Рейтинг: Цена: 18167.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
This Volume addresses the pros and cons of oligonucleotide probes, primers and primer combinations, and importantly considers how to design the best tools for the microbial taxa and/or processes being investigated. Individual chapters focus on the design of primers targeting genes that code for enzymes associated with the following functions: degradation of aromatic, aliphatic and chlorinated hydrocarbons under aerobic and anaerobic conditions, methanogenesis, methane oxidation, and the nitrogen cycle.Hydrocarbon and Lipid Microbiology ProtocolsThere are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.
Автор: Terry J. McGenity; Kenneth N. Timmis; Balbina Noga Название: Hydrocarbon and Lipid Microbiology Protocols ISBN: 366253116X ISBN-13(EAN): 9783662531167 Издательство: Springer Рейтинг: Цена: 19564.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
This Volume describes methods for investigating microbes in their natural environment and how to obtain representative samples and preserve them for subsequent analyses. Chapters are arranged according to the environments under investigation, which include: oil reservoirs, fracking fluids, aquifers, coal beds, oil sands and their tailing ponds, lakes, rivers, leaves, polar seas and ice, the sea-surface microlayer, mud flats, microbialites, and deep-sea fauna. A variety of downstream analytical procedures are described, including: nucleic-acid extraction and preparation for high-throughput sequencing, fluorescence in-situ hybridisation, and cultivation of aerobic and anaerobic hydrocarbon-degrading microbes. Though most chapters focus on hydrocarbon-rich environments, many of the approaches used are generic, and as such will be of value to researchers embarking on studies of microbes and their processes in the field.Hydrocarbon and Lipid Microbiology Protocols There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.
Автор: McGenity Terry J., Timmis Kenneth N., Nogales Balbina Название: Hydrocarbon and Lipid Microbiology Protocols: Biochemical Methods ISBN: 3662569841 ISBN-13(EAN): 9783662569849 Издательство: Springer Рейтинг: Цена: 13974.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This Volume provides protocols for the biochemical analysis of hydrocarbon- and lipid-relevant products, cell components and activities of microbes that interact with hydrophobic compounds.
Автор: Terry J. McGenity; Kenneth N. Timmis; Balbina Noga Название: Hydrocarbon and Lipid Microbiology Protocols ISBN: 366249129X ISBN-13(EAN): 9783662491294 Издательство: Springer Рейтинг: Цена: 18167.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Introduction: Single cell biotechnology for uncultured microorganisms.- Single-cell cultivation in microfluidic devices.- Single-cell bacterial bioreporter assays to measure hydrocarbons.- Whole genome amplification of single bacterial cells.- Detection of specific mRNA molecules in hydrocarbon-degrading bacteria.- Localisation of proteins within intact bacterial cells using fluorescent protein fusions.- Raman-FISH.- Single-cell metabolomics.- Marrying single molecules to single cells: Protocols for the study of the bacterial proto-ring components essential for division.- Single cell biotechnology for uncultured microorganisms.- Single cell and systems biology tools for biofuel production.- Detection of protein-synthesizing microorganisms in the environment via bioorthogonal non-canonical amino acid tagging (BONCAT).- Flow cytometric determination of microbial abundances and its use to obtain indices of community structure and relative activity.- Protocols for visualizing horizontal gene transfer in Gram-negative bacteria through natural competence.
Название: Hydrocarbon and lipid microbiology protocols ISBN: 3662451786 ISBN-13(EAN): 9783662451786 Издательство: Springer Рейтинг: Цена: 19564.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Introduction to the cultivation of microbes involved in the cycling of hydrocarbons.- Cultivating fastidious microbes.- The essentials of cultivating aerobic hydrocarbon-degrading microbes.- Cultivating aerobic hydrocarbon-degrading microbes from microalgae.- Growing and maintaining hydrocarbon-degrading consortia.- Two-phase cultivation techniques for hydrocarbon-degrading organisms.- Cultivation of hydrocarbon-degrading fungi.- Growth of hydrocarbon-degrading bacteria in continuous culture.- Growth procedures at low substrate concentrations.- Cultivation of biofilm-forming hydrocarbon degraders.- Cultivation of asphaltene-degrading microbes.- Cultivation of microorganisms for fuel quality improvement.- Simulation of oxygen and redox oscillations in crude oil- degrading bioreactors.- Cultivation of anaerobic microorganisms with hydrocarbons as growth substrates.- Enrichment and isolation of metal respirers and hydrocarbon oxidizers.- Enrichment and isolation of oxyion-respiring hydrocarbon oxidizers.- Cultivation of methanogens.- Cultivation of methylotrophs.- Cultivation of methanotrophs.- Cultivation of extremophilic hydrocarbon degraders.- Cultivation of piezophilic hydrocarbon degraders.- Cultivation of oil-producing microalgae.- Cultivation of oleaginous yeasts.- Cultivation of oil-tolerant protists.
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