Описание: Introduction to computer-assisted analysis in lipid and hydrocarbon microbiology.- Application of Ecological Network Theory.- Statistical tools for data analysis.- Statistical tools for study design and replication.- MG-RAST, a metagenomics service for analysis of microbial community structure and function.- Using QIIME to evaluate the microbial communities within hydrocarbon environments.- Biodegradation Prediction Tools.- Predicting protein interactions.- Syntax and Semantics of Coding in Python.- Protocols for calculating reaction kinetics and thermodynamics.- Modelling the environmental fate of hydrocarbons during bioremediation.
Описание: This Volume presents protocols for investigating the genetic, metabolic and ecological potential and functional analysis of microbial communities.
Описание: This Volume presents applications of hydrocarbon microbiology in the context of environmental pollutant degradation, covering pollutants such as petroleum and related wastes (i.e.
Автор: Terry J. McGenity; Kenneth N. Timmis; Balbina Noga Название: Hydrocarbon and Lipid Microbiology Protocols ISBN: 366249132X ISBN-13(EAN): 9783662491324 Издательство: Springer Рейтинг: Цена: 16769.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This Volume presents key microscopy and imaging methods for revealing the structure and ultrastructure of environmental and experimental samples, of microbial communities and cultures, and of individual cells.
Автор: McGenity Название: Hydrocarbon and Lipid Microbiology Protocols ISBN: 3662504308 ISBN-13(EAN): 9783662504307 Издательство: Springer Рейтинг: Цена: 18167.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
This Volume presents generic protocols for wet experimental and computer-based systems and synthetic biology approaches relevant to the field of hydrocarbon and lipid microbiology. It complements a second Volume that describes protocols for systems and synthetic biology applications. The wet experimental tools presented in this Volume include protocols for the standardisation of transcriptional measurements, application of uracil excision-based DNA editing for, inter alia, multi-gene assembly, the use of fluxomics to optimise “reducing power availability”, and the incorporation of non-canonical amino acids into proteins for optimisation of activities. Phenome-ing microbes, using a combination of RNA-seq and bioinformatic algorithms, is presented, as is an illustration, using methylotrophs as an example, of how the different key omics approaches constitute a pipeline for functional analysis, acquisition of a systems overview, and metabolic optimisation. Complementary computational tools that are presented include protocols for probing the genome architecture of regulatory networks, genome-scale metabolic reconstruction, and bioinformatic approaches to guide metabolic engineering. The Volume also includes an overview of how synthetic biology approaches can be used to improve biocontainment.
Hydrocarbon and Lipid Microbiology Protocols
There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.
Автор: Terry J. McGenity; Kenneth N. Timmis; Balbina Noga Название: Hydrocarbon and Lipid Microbiology Protocols ISBN: 3662531100 ISBN-13(EAN): 9783662531105 Издательство: Springer Рейтинг: Цена: 18167.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This Volume presents applications of hydrocarbon microbiology in the context of environmental pollutant degradation, covering pollutants such as petroleum and related wastes (i.e.
Автор: Terry J. McGenity; Kenneth N. Timmis; Balbina Noga Название: Hydrocarbon and Lipid Microbiology Protocols ISBN: 3662531135 ISBN-13(EAN): 9783662531136 Издательство: Springer Рейтинг: Цена: 18167.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This Volume covers protocols for various applications in hydrocarbon microbiology, including those of interest for industrial processes, biocatalysis, lipid and biofuel production, bioproducts, or the human microbiome. It presents detailed protocols for the functional screening of enzymes acting on greasy molecules (i.e. lipases, esterases), including assays for enantioselective biocatalysts, as well as approaches for protein display technologies. Protocols for improving fuel quality and production of biofuel and lipids in different hosts (bacteria, algae, yeast) are also provided. The production of biogas from organic waste and its fermentation into value-added products such as polyhydroxyalkanoates is covered, as well as an in-vitro model of the gut microbiome for short-chain fatty acid metabolism and microbial diversity analyses. The applications presented are examples of the many potential applications in hydrocarbon and lipid microbiology, and many (i.e. protein-display technologies) will also be of interest in other research fields. Hydrocarbon and Lipid Microbiology ProtocolsThere are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.
Описание: Introduction to Microbial Quantitation, Community Profiling, and Array Approaches.- A cell extraction method for oily sediments.- Introduction to microplate MPN-enumeration of hydrocarbon degraders.- Primers for dsr genes and Most Probable Number Method for detection of sulfate-reducing bacteria in oil reservoirs.- Microbial biomass and community analysis by PLFA analysis.- Real-time PCR approaches for analysis of hydrocarbon-degrading bacterial communities.- Clone libraries of ribosomal RNA gene sequences for characterization of microbial communities.- Denaturing gradient gel electrophoresis (DGGE) for microbial community analysis.- Microbial community profiling: SSCP and T-RFLP techniques.- Microbial community analysis by single-amplicon high-throughput sequencing (metagenetics) - preparation, data handling and ecological analysis.- Multiplex fluorescent antibody microarrays and antibody graphs for microbial and biomarker detection in the environment.- Studying protistan communities in hydrocarbon-contaminated environments.
Автор: Terry J. McGenity; Kenneth N. Timmis; Balbina Noga Название: Hydrocarbon and Lipid Microbiology Protocols ISBN: 366249129X ISBN-13(EAN): 9783662491294 Издательство: Springer Рейтинг: Цена: 18167.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Introduction: Single cell biotechnology for uncultured microorganisms.- Single-cell cultivation in microfluidic devices.- Single-cell bacterial bioreporter assays to measure hydrocarbons.- Whole genome amplification of single bacterial cells.- Detection of specific mRNA molecules in hydrocarbon-degrading bacteria.- Localisation of proteins within intact bacterial cells using fluorescent protein fusions.- Raman-FISH.- Single-cell metabolomics.- Marrying single molecules to single cells: Protocols for the study of the bacterial proto-ring components essential for division.- Single cell biotechnology for uncultured microorganisms.- Single cell and systems biology tools for biofuel production.- Detection of protein-synthesizing microorganisms in the environment via bioorthogonal non-canonical amino acid tagging (BONCAT).- Flow cytometric determination of microbial abundances and its use to obtain indices of community structure and relative activity.- Protocols for visualizing horizontal gene transfer in Gram-negative bacteria through natural competence.
Автор: McGenity Terry J., Timmis Kenneth N., Nogales Balbina Название: Hydrocarbon and Lipid Microbiology Protocols: Biochemical Methods ISBN: 3662569841 ISBN-13(EAN): 9783662569849 Издательство: Springer Рейтинг: Цена: 13974.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This Volume provides protocols for the biochemical analysis of hydrocarbon- and lipid-relevant products, cell components and activities of microbes that interact with hydrophobic compounds.
Автор: McGenity Terry J., Timmis Kenneth N., Nogales Balbina Название: Hydrocarbon and Lipid Microbiology Protocols: Meso- And Microcosms ISBN: 3662571129 ISBN-13(EAN): 9783662571125 Издательство: Springer Рейтинг: Цена: 13974.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: < Hydrocarbon and Lipid Microbiology ProtocolsThere are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function.
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