Описание: From probe design to applications in clinical settings, this book provides a diverse set of instructive examples, guided by experts in the field who offer easy-to-follow experimentals.
Автор: Viktor Stein Название: Synthetic Protein Switches ISBN: 1493969382 ISBN-13(EAN): 9781493969388 Издательство: Springer Рейтинг: Цена: 16769.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Beginning with general strategies, the volume continues with coverage of peptide switches, fluorescent and bioluminescent sensors, ss-lactamase sensors, proteolytic sensors, optogenetic switches, as well as cellular signaling switches.
Описание: The orchid family is one of the largest families of flowering plants known for their beauty and economic importance. This work provides information in key areas of research that are important to both scientists and commercial growers alike. The main purposes of this book are to provide key practical areas of research, such as, germination, micropropagation, traditional and current techniques related to plant improvement; document methods that ensure survival of plants from laboratories to greenhouses; promote communication between scientists and growers, so that their combined expertise on these areas will lead to the successful growth of orchids in their natural habitats or commercial greenhouses. This book can serve as reference for laymen with an interest in orchid growing.
This book is divided into 5 parts. The first part emphasizes propagation methods using seeds and related techniques that are important to plant conservation and improvement. Successes in asymbiotic and symbiotic seed germination are keys to orchid conservation and their propagation. The second part summarizes micropropagation methods, common media, and newer methods of micropropagation such as the bioreactor culture procedures. The third part focuses on techniques related to the manipulation of explants in an in vitro environment. The fourth part covers cell biological methods and transformation techniques. Since the successes in a laboratory setting do not guarantee plant survival and propagation in greenhouses and in the natural environment, it discusses greenhouse propagation techniques that are essential to the survival of plants generated from a laboratory setting. The fifth part showcases recent successes on orchid propagation by documenting sample publications and how to present orchids in an artistic fashion for one’s enjoyment.
Автор: Marchisio Mario Andrea Название: Computational Methods in Synthetic Biology ISBN: 1071608215 ISBN-13(EAN): 9781071608210 Издательство: Springer Цена: 18167.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume in the Encyclopedia of Sustainability Science and Technology, Second Edition, describes the breadth of science and engineering knowledge critical to advancing sustainable built environments, from architecture and design, mechanical engineering, lighting, and materials to water and energy, public policy, and economics.
Автор: Janzen William P. Название: High Throughput Screening: Methods and Protocols ISBN: 1493981137 ISBN-13(EAN): 9781493981137 Издательство: Springer Рейтинг: Цена: 15902.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
1. Design and Implementation of High Throughput Screening Assays David J. Powell, Robert P. Hertsberg, and Ricardo Macarrόn 2. Characterization of Inhibitor Binding Through Multiple Inhibitor Analysis: A Novel Local Fitting Method Thomas V. Riera, Tim J. Wigle, and Robert A. Copeland
3. High Throughput Screening Using Mass Spectrometry within Drug Discovery Mattias Rohman and Jonathan Wingfield 4. Structure-Based Virtual Screening of Commercially Available Compound Libraries Dmitri Kireev 5. AlphaScreen-Based Assays: Ultra-High Throughput Screening for Small Molecule Inhibitors of Challenging Enzymes and Protein-Protein Interactions Adam Yasgar, Ajit Jadhav, Anton Simeonov, and Nathan P. Coussens 6. Instrument Quality Control Chatura Jayakody and Emily A. Hull-Ryde 7. Application of Fluorescence Polarization in HTS AssaysXinyi Huang and Ann Aulabaugh 8. Time Resolved Fluorescence Assays Chen-Ting Ma and Eduard A. Sergienko 9. Protein Kinase Selectivity Profiling Using Microfluid Mobility Shift Assays Peter Drueckes 10. Screening for Inhibitors of Kinase Autophosphorylation Bianca Heedmann and Martin Klumpp 11. A Fluorescence-Based High Throughput Screening Assay to Identify Growth Inhibitors of the Pathogenic Fungus Aspergillus fumigatus Thomas M. Smith, Daryl L. Richie, Jianshi Tao 12. Mycobacterium tuberculosisHigh Throughput Screening E. Lucile White, Nichole A. Tower, and Lynn Rasmussen 13. Identification of State-Dependent Blockers for Voltage Gated Calcium Channels Using a FLIPR Based Assay Alberto di Silvio 14. A Luciferase Reporter Gene System for High Throughput Screening of γ-GlobinGene Activators Wensheng Xie, Robert Silvers, Michael Ouellette, Zining Wu, Quinn Lu, Hu Li, Kathleen Gallagher, Kathy Johnson, and Monica Montoute 15. A High Throughput Flow Cytometry Assay for Identification of Inhibitors of 3', 5'-Cyclic Adenosine Monophosphate Efflux Dominique Perez, Peter C. Simons, Yelena Smagley, Larry A. Sklar, and Alexandre Chigaev 16. High Throughput Cell Toxicity Assays David Murray, Lisa McWilliams, and Mark Wigglesworth 17. BRETn: NanoLuc-Based Bioluminescence Resonance Energy Transfer Platform to Monitor Protein-Protein Interactions in Live Cells Xiu-Lei Mo and Haian Fu 18. Application of Imaging-Based Assays in Microplate Formats for High Content Screening Adam I. Fogel, Scott E. Martin, and Samuel A. Hasson
Автор: Pfeifer Blaine A., Hill Andrew Название: Vaccine Delivery Technology: Methods and Protocols ISBN: 1071607944 ISBN-13(EAN): 9781071607947 Издательство: Springer Рейтинг: Цена: 18167.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume discusses the vaccine development process and the role delivery concepts contribute to a global goal of effective health outcomes.
Автор: Opara Emmanuel C. Название: Cell Microencapsulation: Methods and Protocols ISBN: 1493981773 ISBN-13(EAN): 9781493981779 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
Part IIntroduction
1. Historical Perspectives and Current Challenges in Cell Microencapsulation Paul de Vos 2. Applications of Cell Microencapsulation Emmanuel C. Opara Part IIApproaches to Cell Microencapsulation 3. Cell Microencapsulation: Dripping Methods A. Bidoret, E. Martins, B. Poncelet De Smet, and D. Poncelet 4. Field Effect Microparticle Generation for Cell Microencapsulation Brend Ray-Sea Hsu and Shin-Huei Fu 5. Microfluidic Approach to Cell Microencapsulation Varna Sharma, Michael Hunckler, Melur K. Ramasubramanian, Emmanuel C. Opara, and Kalyan C. Katuri Part III Biomaterials and Enabling Technologies in Cell Microencapsulation 6. Polymeric Materials for Cell Microencapsulation A. Aijaz, D. Perera, and R. Olabisi William F. Kendall and Emmanuel C. Opara 8. Determination of the Mechanical Strength of Microcapsules Marcus D. Darrabie and Emmanuel C. Opara 9. The Diffusive Properties of Hydrogel Microcapsules for Cell Encapsulation D.M. Lavin, B.E. Bintz, and C.G. Thanos 10. Methods for Incorporating Oxygen Generating Biomaterials into Cell Culture and Microcapsule Systems John Patrick McQuilling and Emmanuel C. Opara 11. Non-Invasive Tracking of Alginate-Microencapsulated Cells Genaro A. Paredes-Juarez, Brad P. Barnett, and Jeff W.M. Bulte 12. Retrieval of Microencapsulated Islet Grafts for Post-Transplant Evaluation John Patrick McQuilling, Sivanandane Sittadjody, Rajesh Pareta, Samuel Pendergraft, Clancy J. Clark, Alan C. Farney, and Emmanuel C. Opara Part IV Isolation of Cells for Microencapsulation 13. A Method of Porcine Pancreatic Islet Isolation for Microencapsulation William F. Kendall and Emmanuel C. Opara 14: Selective Osmotic Shock (SOS)-Based Islet Isolation for Microencapsulation Kevin Enck, John P. McQuilling, Giuseppe Orlando, Riccardo Tamburrini, Sittadjody Sivanandane, and Emmanuel C. Opara 15. Preparation and Characterization of Alginate-Chitosan Microcapsule for Hepatocyte Culture Lamjuan Li, Yanhong Zhang, and Xiaoping Pan
16. Use of Flow Focusing Technique for Microencapsulation of Myoblasts J. Ciriza, L. Saenz del Burgo, RM Hernandez, G. Orive, and JL Pedraz
17. Alginate Microbeads for Cell and Protein Delivery Sami I. Somo, Omaditya Khanna, and Eric M. Brey
18. Compartmentalization of Two Cell Types in Multi-Layered Alginate Microcapsules Sivanandane Sittadjody, Justin M. Saul, and Emmanuel C. Opara 19. Primary Choroid Plexus Tissue for Use in Cellular Therapy M.A. Sandrof and C.G. Thanos 20. Microencapsulation of Stem Cells for Therapy Shirae K. Leslie, Ramsey C. Kinney, Zvi Schwartz, and Barbara D. Boyan
21. Microencapsulated Cells for Cancer Therapy L. Saenz del Burgo, J. Ciriza, RM Hernбndez, G. Orive, and JL Pedraz 22. Microencapsulation of Bacterial Cells by Emulsion Technique for Probiotic Application Surajit Mandal and Subrota Hati Part V Cell Microencapsulation in Clinical Applications 23. Microencapsulation of Islets for the Treatment of Type 1 Diabetes Mellitus (T1D) Riccardo Calafiore, Giuseppe Basta, and Pia Montanucci
Автор: Ebrahimkhani Mo R., Hislop Joshua Название: Programmed Morphogenesis: Methods and Protocols ISBN: 1071611739 ISBN-13(EAN): 9781071611739 Издательство: Springer Цена: 15372.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume in the Encyclopedia of Sustainability Science and Technology, Second Edition, describes the breadth of science and engineering knowledge critical to advancing sustainable built environments, from architecture and design, mechanical engineering, lighting, and materials to water and energy, public policy, and economics.
Автор: Ogris Manfred, Oupicky David Название: Nanotechnology for Nucleic Acid Delivery: Methods and Protocols ISBN: 1493959328 ISBN-13(EAN): 9781493959327 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This Methods in Molecular Biology (TM) series book covers nanoparticulate delivery of nucleic acids in terms of biosafety, particle synthesis as well as its application in cell culture. Includes materials and reagents, protocols, notes on troubleshooting and more.
Автор: Narayanan Kumaran Название: Bio-Carrier Vectors: Methods and Protocols ISBN: 1071609424 ISBN-13(EAN): 9781071609422 Издательство: Springer Цена: 13974.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This detailed book explores techniques used in research efforts to adopt and combine the best capabilities from natural and artificial vector systems to assemble improved delivery technologies aimed for specific applications.
Автор: Himmel Michael E. Название: Biomass Conversion: Methods and Protocols ISBN: 1493959263 ISBN-13(EAN): 9781493959266 Издательство: Springer Рейтинг: Цена: 18167.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Biomass conversion research is a combination of basic science, applied science, and engineering testing and analysis. In Biomass Conversion: Methods and Protocols, expert researchers in the field detail methods which are now commonly used to study biomass conversion.
Автор: Domingues, Lucilia Название: PCR Methods and Protocols ISBN: 1493970593 ISBN-13(EAN): 9781493970599 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: 1. In silico PCR Tools for a Fast Primer, Probe and Advanced Searching
Ruslan Kalendar, Alexandr Muterko, Malika Shamekova, and Kabyl Zhambakin
2. Introduction on using the FastPCR Software and the Related Java Web Tools for PCR and Oligonucleotide Assembly and Analysis
Ruslan Kalendar and Timofey V. Tselykh
3. Long Fragment Polymerase Chain Reaction
Eng Wee Chua, Simran Maggo, and Martin A. Kennedy
4. Strategies to Improve Efficiency and Specificity of Degenerate Primers in PCR
Maria Jorge Campos and Alberto Quesada
5. Inverse PCR for Point Mutation Introduction
Diogo Silva, Gustavo Santos, Mбrio Barroca, and Tony Collins
6. Synthesis of Fusion Genes for Cloning by Megaprimer-based PCR
Tatiana Q. Aguiar, Carla Oliveira, and Lucнlia Domingues
7. A Novel Platform for High-throughput Gene Synthesis to Maximize Recombinant Expression in Escherichia coli
Ana Filipa Sequeira, Joana L.A. Brбs, Vвnia O. Fernandes, Catarina I.P.D. Guerreiro, Renaud Vincentelli, and Carlos M.G.A. Fontes
Flбvio Azevedo, Humberto Pereira, and Bjцrn Johansson
9. CRENAME, a Molecular Microbiology Method Enabling Multiparametric Assessment of Potable/Drinking Water
Luc Bissonnette, Andrйe F. Maheux, and Michel G. Bergeron
10. Multiplex Detection Of Food-borne Pathogen
Germбn Villamizar-Rodrнguezand Felipe Lombу
11. Fast Real-time PCR for the Detection of Crustacean Allergens in Foods
Francisco J. Santaclara and Montserrat Espiсeira
12. Fast Real-time PCR Method for the Detection of Soy in Foods
Montserrat Espiсeira and Francisco J. Santaclara
13. RAPD/SCAR Approaches for Identification of Adulterant's Breed's Milk in Dairy Products
Joana Cunha and Lucнlia Domingues
14. Genetic Diversity Analysis of Medicinally Important Horticultural Crop Aegle marmelos by ISSR Markers
Farina Mujeeb, Preeti Bajpai, Neelam Pathak, and Smita Rastogu Verma
15. PCR in the Analysis of Clinical Samples: Prenatal and Postnatal Diagnosis of Inborn Errors of Metabolism
Laura Vilarinho and Cйlia Nogueira
16. Harnessing the Power of PCR-dependent Molecular Fingerprinting Methods for Understanding Structure and Function in Microbial Communities
S.S. Phadke, A.F.Salvador, J.I. Alves, O. Bretschger, M.M. Alves, and M.A. Pereira
17. PCR in Metagenomics
Sarita G Bhat and Tina K. J.
18. Arbitrarily Primed PCR for Comparison of Metagenomes and Extracting useful loci from them
Leigh Burgoyne, Lin Y Koh, and David Catcheside
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