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Recombinant Protein Production in Yeast: Methods and Protocols, Bill Roslyn M.


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Автор: Bill Roslyn M.
Название:  Recombinant Protein Production in Yeast: Methods and Protocols
ISBN: 9781493958719
Издательство: Springer
Классификация:


ISBN-10: 1493958712
Обложка/Формат: Paperback
Страницы: 248
Вес: 0.46 кг.
Дата издания: 23.08.2016
Серия: Methods in molecular biology
Язык: English
Издание: Softcover reprint of
Иллюстрации: 22 tables, black and white; 6 illustrations, color; 41 illustrations, black and white; xii, 248 p. 47 illus., 6 illus. in color.; 22 tables, black and
Размер: 25.40 x 17.78 x 1.40 cm
Читательская аудитория: General (us: trade)
Подзаголовок: Methods and protocols
Ссылка на Издательство: Link
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Поставляется из: Германии
Описание: In the last few years, significant advances have been made in understanding how a yeast cell responds to the stress of producing a recombinant protein, and how this information can be used to engineer improved host strains. The molecular biology of the expression vector, through the choice of promoter, tag and codon optimization of the target gene, is also a key determinant of a high-yielding protein production experiment. Recombinant Protein Production in Yeast: Methods and Protocols examines the process of preparation of expression vectors, transformation to generate high-yielding clones, optimization of experimental conditions to maximize yields, scale-up to bioreactor formats and disruption of yeast cells to enable the isolation of the recombinant protein prior to purification. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Recombinant Protein Production in Yeast: Methods and Protocols, seeks to aid scientists in adopting yeast as a protein production host.
Дополнительное описание: Optimising Yeast as a Host for Recombinant Protein Production( review).-Which Yeast Species Shall I Choose? Saccharomyces cerevisiae versus Pichia pastoris (review).-Preparation of Pichia pastoris Expression Plasmids.-Preparation of Saccharomyces cerevisi



Recombinant Glycoprotein Production

Автор: Virg?nia Pican?o-Castro; Kamilla Swiech
Название: Recombinant Glycoprotein Production
ISBN: 1493984497 ISBN-13(EAN): 9781493984497
Издательство: Springer
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Цена: 15372.00 р.
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Описание: This volume covers a wide spectrum of techniques and approaches that are used in the upstream and downstream processing for recombinant glycoprotein production. Chapters guide the reader through state-of-art of therapeutic recombinant glycoproteins, explores the patent literature, expression systems used for glycoproteins production, methods employed in the downstream processing of different glycoproteins, and information about analytical tools and formulation strategies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Recombinant Glycoprotein Production: Methods and Protocols aims to ensure successful results in the further study of this vital field

Recombinant Protein Production in Yeast

Автор: Brigitte Gasser; Diethard Mattanovich
Название: Recombinant Protein Production in Yeast
ISBN: 1493990233 ISBN-13(EAN): 9781493990238
Издательство: Springer
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Цена: 18167.00 р.
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Описание:

This volume provides an overview of the main yeast production platforms currently used and future yeast cell factories for recombinant protein production. Chapters detail approaches of genetic and metabolic engineering, co-factor containing proteins and virus-like particles, glycoproteins, and post-translational modifications of proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Recombinant Protein Production in Yeast: Methods and Protocols aims to provide state of the art background and methods for protein producing yeast platforms, as well as case studies for special applications.
Recombinant Protein Production with Prokaryotic and Eukaryotic Cells. A Comparative View on Host Physiology

Автор: Otto-Wilhelm Merten; D. Mattanovich; C. Lang; G. L
Название: Recombinant Protein Production with Prokaryotic and Eukaryotic Cells. A Comparative View on Host Physiology
ISBN: 0792371372 ISBN-13(EAN): 9780792371373
Издательство: Springer
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Цена: 34799.00 р.
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Описание: Selected Articles from the Meeting of the EFB Section on Microbial Physiology, Semmering/A, 5-8 October 2000

Recombinant Protein Expression in Mammalian Cells

Автор: David L. Hacker
Название: Recombinant Protein Expression in Mammalian Cells
ISBN: 1493993771 ISBN-13(EAN): 9781493993772
Издательство: Springer
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Цена: 16070.00 р.
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Описание: This detailed volume explores advances in vector design, DNA delivery, cell cultivation, host cell engineering, and bioprocess optimization within the study of recombinant protein expression in mammalian cells. The majority of the protocols employ either Chinese hamster ovary cells (CHO) or human embryonic kidney 293 cells (HEK293), the workhorses of the field, as the production host; however, the methods can be adapted to other mammalian hosts under the appropriate cell-specific conditions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and convenient, Recombinant Protein Expression in Mammalian Cells: Methods and Protocols aims to aid researchers in building on our knowledge of protein structure and function and to speed the discovery of new therapeutic proteins.

Recombinant Technology in Hemostasis and Thrombosis

Автор: W.N. Drohan; Leon W. Hoyer
Название: Recombinant Technology in Hemostasis and Thrombosis
ISBN: 1461366445 ISBN-13(EAN): 9781461366447
Издательство: Springer
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Цена: 6986.00 р.
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Описание: The speakers described the essential features of the genes responsible for key plasma proteins important in hemostasis, including procoagulant Factors VIII and IX and anticoagulant proteins, Antithrombin III and Protein C.

Recombinant Protein Expression in Mammalian Cells

Автор: Hacker
Название: Recombinant Protein Expression in Mammalian Cells
ISBN: 1493987291 ISBN-13(EAN): 9781493987290
Издательство: Springer
Рейтинг:
Цена: 16070.00 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание: This detailed volume explores advances in vector design, DNA delivery, cell cultivation, host cell engineering, and bioprocess optimization within the study of recombinant protein expression in mammalian cells. The majority of the protocols employ either Chinese hamster ovary cells (CHO) or human embryonic kidney 293 cells (HEK293), the workhorses of the field, as the production host; however, the methods can be adapted to other mammalian hosts under the appropriate cell-specific conditions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and convenient, Recombinant Protein Expression in Mammalian Cells: Methods and Protocols aims to aid researchers in building on our knowledge of protein structure and function and to speed the discovery of new therapeutic proteins.

Recombinant and In Vitro RNA Synthesis

Автор: Graeme L. Conn
Название: Recombinant and In Vitro RNA Synthesis
ISBN: 1493958305 ISBN-13(EAN): 9781493958306
Издательство: Springer
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Цена: 16769.00 р.
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Описание: Part of Springer`s Methods in Molecular Biology series, this collection of detailed protocols guides the reader from the start of a new RNA project to the final sample, with step-by-step protocols and expert tips on deploying catalytic RNAs in the laboratory.

Recombinant Proteins from Plants

Автор: Jacqueline MacDonald; Igor Kolotilin; Rima Menassa
Название: Recombinant Proteins from Plants
ISBN: 1493932888 ISBN-13(EAN): 9781493932887
Издательство: Springer
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Цена: 13275.00 р.
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Описание:

This volume provides up-to-date scientific achievements from the world's top researchers. Recombinant Proteins from Plants: Methods and Protocols, Second Edition guides readers through protocolsfor use with a variety of plant expression systems. Various aspects of production are covered including vector selection and cloning; product improvements for stability, glycosylation, and antibiotic-free selection; extraction and scale-up; and analysis of transgenic plants and their recombinant proteins. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Recombinant Proteins from Plants: Methods and Protocols, Second Edition is an ideal reference for those who are interested in plant molecular biology and molecular farming.

Recombinant Gene Expression

Автор: Argelia Lorence
Название: Recombinant Gene Expression
ISBN: 1493962213 ISBN-13(EAN): 9781493962211
Издательство: Springer
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Цена: 23058.00 р.
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Описание: Recombinant gene expression research has brought new advances such as the birth of the `omics` technologies. Here, expert researchers detail many RGE methods now used, including those for bacteria, fungi, plants and plant cells, and animals and animal cells.

Recombinant Protein Production with Prokaryotic and Eukaryotic Cells. A Comparative View on Host Physiology

Автор: Otto-Wilhelm Merten; D. Mattanovich; C. Lang; G. L
Название: Recombinant Protein Production with Prokaryotic and Eukaryotic Cells. A Comparative View on Host Physiology
ISBN: 9048157560 ISBN-13(EAN): 9789048157563
Издательство: Springer
Рейтинг:
Цена: 34799.00 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание: Selected Articles from the Meeting of the EFB Section on Microbial Physiology, Semmering/A, 5-8 October 2000

Recombinant Glycoprotein Production

Автор: Virg?nia Pican?o-Castro; Kamilla Swiech
Название: Recombinant Glycoprotein Production
ISBN: 1493973118 ISBN-13(EAN): 9781493973118
Издательство: Springer
Рейтинг:
Цена: 19564.00 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание: Chapters guide the reader through state-of-art of therapeutic recombinant glycoproteins, explores the patent literature, expression systems used for glycoproteins production, methods employed in the downstream processing of different glycoproteins, and information about analytical tools and formulation strategies.

Recombinant enzymes - from basic science to commercialization

Название: Recombinant enzymes - from basic science to commercialization
ISBN: 3319123963 ISBN-13(EAN): 9783319123967
Издательство: Springer
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Цена: 19591.00 р.
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Описание: Recombinant Enzymes From Basic Science to Commercialization


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