Rnai and Small Regulatory Rnas in Stem Cells: Methods and Protocols, Zhang Baohong
Автор: Baohong Zhang Название: RNAi and Small Regulatory RNAs in Stem Cells ISBN: 1493971069 ISBN-13(EAN): 9781493971060 Издательство: Springer Рейтинг: Цена: 20962.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
Part I: Introduction
1. RNAi Technique in Stem Cell Research: Current Status and Future Perspectives
Gang-Ming Zou
2. RNAi and MicroRNA-Mediated Gene Regulation in Stem Cells
Alexander K. Murashov
Part II: RNA Interference
3. Forward RNAi Screens in Human Hematopoietic Stem Cells
Roman Galeev, Christine Karlsson, Aurйlie Baudet, and Jonas Larsson
4. Silencing of ATP11B by RNAi-Induced Changes in Neural Stem Cell Morphology
5. High-Efficiency Transfection of Glioblastoma Cells and a Simple Spheroid Migration Assay
Carsten Hagemann, Diana Amend, Almuth F. Kessler, Thomas Linsenmann, Ralf-Ingo Ernestus, and Mario Lцhr
6. Construction and Application of Random dsRNA Interference Library for Functional Genetic Screens in Embryonic Stem Cells
Xiaoxing Cheng and Rui Jian
7. Efficient Depletion of Essential Gene Products for Loss-of-Function Studies in Embryonic Stem Cells
Soizik Berlivet, Isabelle Hmitou, Hйlиne Picaud, and Matthieu Gйrard
8. Regulation and/or Repression of Cholinergic Differentiation of Murine Embryonic Stem Cells Using RNAi Directed Against Transcription Factor L3/Lhx8
Takayuki Manabe and Akio Wanaka
9. A Recessive Genetic Screen for Components of the RNA Interference Pathway Performed in Mouse Embryonic Stem Cells
Melanie I. Trombly and Xiaozhong Wang
10. RNAi Knockdown of Ape1 Gene in the Differentiation of Mouse Embryonic Stem Cells
Gang-Ming Zou, Jieqing Yu, Cynthia LeBron, and Yumei Fu
11. An Efficient Transfection Method for Differentiation and Cell Proliferation of Mouse Embryonic Stem Cells
Jun-Yang Liou, Bor-Sheng Ko, and Tzu-Ching Chang
12. Implanting Glioblastoma Spheroids into Rat Brains and Monitoring Tumor Growth by MRI Volumetry
Mario Lцhr, Thomas Linsenmann, Anna Jawork, Almuth F. Kessler, Nils Timmermann, Gyцrgy A. Homola, Ralf-Ingo Ernestus, and Carsten Hagemann
13. RNAi-Based Techniques for the Analysis of Gene Function in Drosophila Germline Stem Cells
Amelia J. Helms, Danielle S. Finger, Victoria L. Hardy, and Elizabeth T. Ables
14. In Vivo RNAi in the Drosophila Follicular Epithelium: Analysis of Stem Cell Maintenance, Proliferation, and Differentiation
Veit Riechmann
15. A Phenotype-Based RNAi Screening for Ras-ERK/MAPK Signaling-Associated Stem Cell Regulators in C. elegans
Myon-Hee Lee and Dong Suk Yoon
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Автор: Zhang Название: RNAi and microRNA-Mediated Gene Regulation in Stem Cells ISBN: 1607617684 ISBN-13(EAN): 9781607617686 Издательство: Springer Рейтинг: Цена: 22359.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This cutting-edge text will be a valuable resource for scientists and aspiring graduate students interested in the intersection of RNAi, miRNA, and stem cell molecular biology and exciting areas of research such as regenerative medicine and cancer.
Автор: Baohong Zhang; Edmund J. Stellwag Название: RNAi and microRNA-Mediated Gene Regulation in Stem Cells ISBN: 149395749X ISBN-13(EAN): 9781493957491 Издательство: Springer Рейтинг: Цена: 19564.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Recent stem cell research has revealed that miRNA and RNAi-mediated gene regulation is one of the vital determinates controlling the state of cell differentiation, with the small RNAs serving as key elements involved in regulatory network control of pluripotent cell fate determination. In RNAi and microRNA-Mediated Gene Regulation in Stem Cells: Methods, Protocols, and Applications, expert authors from laboratories across the globe contribute an accessible compendium of up-to-date, proven methods focused on the study of the titular topic. Divided into three sections, the book first gives a brief introduction to RNAi and miRNAs in stem cells, with a focus on the current status of research and future perspectives, then it continues with detailed methods and protocols for RNAi screening, transfection, and the knockdown of specific genes and pathways in several animal species, including humans and mice, concluding with a section on recently developed methods for identification of miRNAs, including a general protocol for preparation and analysis of miRNA libraries for deep sequencing, knock down of a specific gene using miRNA-based shRNA, and miRNA expression analysis using qRT-PCR. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes highlighting tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, RNAi and microRNA-Mediated Gene Regulation in Stem Cells: Methods, Protocols, and Applications serves as a valuable resource for scientists and aspiring graduate students interested in the intersection of RNAi, miRNA, and stem cell molecular biology and the exciting areas of medicine, including regenerative medicine, aging, cancer, and neurological disorders, that can be advanced through this expanding area of research.
Название: RNAi Technology ISBN: 1138114340 ISBN-13(EAN): 9781138114340 Издательство: Taylor&Francis Рейтинг: Цена: 10717.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: RNAi technology are used for large-scale screens that systematically shut down each gene in the cell, which can help identify the components necessary for a particular cellular process or an event such as cell division. Exploitation of the pathway is also a promising tool in biotechnology and medicine. This book reviews the selective and robust effect of RNAi on gene expression which makes it a valuable research tool, both in cell culture and in living organisms because synthetic dsRNA introduced into cells can induce suppression of specific genes of interest. This book will introduce new technology in the study of RNA interference in microorganisms, plants and animals.
Автор: Bibekanand Mallick; Zhumur Ghosh Название: Regulatory RNAs ISBN: 3642442846 ISBN-13(EAN): 9783642442841 Издательство: Springer Рейтинг: Цена: 23508.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Regulatory RNAs are key players in a number of cellular functions and thus are vital to the development of therapeutic and molecular medicine. This comprehensive study of the topic includes theoretical and historical perspectives as well as applications.
Название: Regulation of Gene Expression by Small RNAs ISBN: 1138111732 ISBN-13(EAN): 9781138111738 Издательство: Taylor&Francis Рейтинг: Цена: 11482.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
New Findings Revolutionize Concepts of Gene Function
Endogenous small RNAs have been found in various organisms, including humans, mice, flies, worms, fungi, and bacteria. Furthermore, it's been shown that microRNAs acting as cellular rheostats have the ability to modulate gene expression. In higher eukaryotes, microRNAs may regulate as much as 50 percent of gene expression.
Regulation of Gene Expression by Small RNAs brings together the pioneering work of researchers who discuss their work involving a wide variety of small RNA regulatory pathways in organisms ranging from bacteria to humans. In addition to exploring the biogenesis and processing of these regulatory RNAs, they also consider the functional importance of these pathways in host organisms. Assisting current and future researchers, this unique groundbreaking work --
Provides a suite of cutting-edge resources for the study of microRNA ontology and function
Includes a technology guide for those seeking to assay microRNA expression
Explores the mechanisms by which microRNAs regulate gene expression in animal cells, including the regulation of gene expression by RNA-mediated transcriptional gene silencing
Discusses a fast and low-cost approach for reversing genetic influences in mammals
Looks at breakthroughs in the use of microRNA-based therapy for HIV and cancer
This volume captures the essence of the breadth and excitement surrounding the newly discovered regulatory roles of small RNAs. The powerful new approach in the study of gene function described in this text is leading to some remarkable findings that have the potential to revolutionize our understanding of genetic function and the treatment of diseases otherwise considered intractable.
Автор: Perdiguero Eusebio, Cornelison Ddw Название: Muscle Stem Cells: Methods and Protocols ISBN: 1493982966 ISBN-13(EAN): 9781493982967 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume provides leading-edge protocols in the study of the molecular and cellular biology of muscle stem cells.
Автор: Di Nardo Paolo, Dhingra Sanjiv, Singla Dinender K. Название: Adult Stem Cells: Methods and Protocols ISBN: 1493982931 ISBN-13(EAN): 9781493982936 Издательство: Springer Рейтинг: Цена: 16769.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
Preface... Table of Contents... Contributing Authors...
1. A Simple Protocol to Isolate, Characterize and Expand Dental Pulp Stem Cells Federica Di Scipio, Andrea Elio Sprio, Maria Elisabetta Carere, Zhiqian Yang, and Giovanni Nicolao Berta 2. Derivation of Mesenchymal Stem cells from Embryonic Stem Cells: A Non-Variable and Inexhaustive Source of Adult Stem Cells Glen Lester Sequiera, Niketa Sareen, Ejlal Abu Al-Rub, and Sanjiv Dhingra 3. Compact Bone-Derived Multipotent Mesenchymal Stromal Cells (MSCs) for the Treatment of Sjogren's-Like Disease in NOD Mice Ghada Abu Elghanam, Younan Liu, Saeed Khalili, Dongdong Fang, and Simon D.Tran 4. Manipulating the Proliferative Potential of Cardiomyocytes by Gene Transfer Giulia Prosdocimo and Mauro Giacca 5. Assessment of Energy Metabolic Changes in Adipose Tissue-Derived Stem Cells Ghazaleh Hajmousa and Martin C. Harmsen 6. Isolation and In Vitro Characterization of Epidermal Stem Cells Kasper S. Moestrup, Marianne S. Andersen, and Kim B. Jensen 7. Endothelial Progenitor Cells: Procedure for Cell Isolation and Characterization Garikipati V.N. Srikanth, Zhongjian Cheng, and Raj Kishore 8. Therapeutic Application of Placental Mesenchymal Stem Cells Reprogrammed Neurospheres in Spinal Cord Injury of SCID Vikram Sabapathy, Franklin Jebaraj Herbert, and Sanjay Kumar 9. Therapeutic Application of Human Wharton Jelly Mesenchymal Stem Cells in Skin Injury of SCID Vikram Sabapathy, Balasubramanian Sundaram, and Sanjay Kumar 10. Isolation, Characterization, and Expansion of Cancer Stem Cells Luke A. Torre-Healy, Artem Berezovsky, and Justin D. Lathia 11. Cardiac Progenitor Cell Isolation from Human Auricles Paolo Di Nardo and Francesca Pagliari 12. Isolation and Culture of Satellite Cells from Mouse Skeletal Muscle Antonio Musarт and Silvia Carosio 13. Isolation of Stromal Stem Cells from Adipose Tissue Maria Prat, Francesca Oltolina, Silvia Antonini, and Andrea Zamperone 14. Human Induced Pluripotent Stem Cell-Derived Mesencymal Stem Cells as an Individual-Specific and Renewable Source of Adult Stem Cells15. A Simplified and Systematic Method to Isolate, Culture and Characterize Multiple Types of Human Dental Stem Cells from a Single Tooth Mohammed Bakkar, Younan Liu, Dongdong Fang, Camille Stegen, Xinyun Su, Murali Ramamoorthi, Li-Chieh Lin, Takako Kawasaki, Nicholas Makhoul, Huan Pham, Yoshinori Sumita, and Simon D. Tran 16. Measurement of Autophagy by Flow Cytometry Silvia Zappavigna, Angela Lombardi, Gabriella Misso, Anna Grimaldi, and Michele Caraglia 17. Echocardiography-Guided Intramyocardial Injection Method in a Murine Model Kay Maeda, Rick Seymour, Marc Ruel, and Erik J. Suuronen 18. Noninvasive Assessment of Cell Fate and Biology in Transplanted Mesenchymal Stem Cells Federico Franchi and Martin Rodriguez-Porcel 19. Methods for Long Term Storage of Murine Bone Marrow Derived Mesenchymal Stem Cells Niketa Sareen, Ejlal Abu-El-Rub, Glen Lester Sequiera, Meenal Moudgil, and Sanjiv Dhingra 20. Therapeutic Application of Adult Stem Cells in the Heart Taylor A. Johnson and Dinender K. Singla 21. Patient-Derived and Intra-Operatively Formed Biomaterial for Tissue Engineering Shalmli U. Joshi, Rares O. Barbu, Melissa Carr-Reynolds, Brian Barnes, and Syam P. Nukavarapu 22. Three-Dimensional Printed Scaffolds with Multipotent Mesenchymal Stromal Cells for Rabbit Mandibular Reconstruction and Engineering Dongdong Fang, Michael Roskies, Mohamed-Nur Abdallah, Mohammed Bakkar, Jack Jordan, Li-Chieh Lin, Faleh Tamimi, and Simon D. Tran 23. Optimal Enviro
1. Introduction to Hair-Follicle-Associated Pluripotent (HAP) Stem Cells Robert M. Hoffman 2. Nestin-Based Reporter Transgenic Mouse Lines John Mignone, Natalia Peunova, and Grigori Enikolopov 3. Discovery of HAP Stem Cells Lingna Li and Robert M. Hoffman 4. Peripheral-Nerve and Spinal-Cord Regeneration in Mice Using Hair Follicle-Associated-Pluripotent (HAP) Stem Cells Yasuyuki Amoh, Kensei Katsuoka, and Robert M. Hoffman 5. Construction of Tissue Engineered Nerve Conduits Seeded with Neurons Derived from Hair Follicle Neural Crest Stem Cells Fang Liu, Haiyan Lin, and Chuansen Zhang 6.Nestin-Expressing Hair Follicle-Associated Pluripotent (HAP) Stem Cells Promote Whisker Sensory-Nerve Growth in Long-Term 3D-Gelfoam Histoculture Sumiyuki Mii, Jennifer Duong, Yasunori Tome, Aisada Uchugonova, Fang Liu, Yasuyuki Amoh, Norimitsu Saito, Kensei Katsuoka, and Robert M. Hoffman 7. Isolation and Culture of Neural Crest Stem Cells from Human Hair Follicles Ruifeng Yang and Xiaowei Xu
8. Isolation of Mouse Hair Follicle Bulge Stem Cells and their Functional Analysis in a Reconstitution Assay Ying Zheng, Jen-Chih Hsieh, Julia Escandon, and George Cotsarelis 9.Hair Follicle Regeneration by Transplantation of a Bioengineered Hair Follicle Germ Katsunari Tezuka, Koh-ei Toyoshima, and Takashi Tsuji 10. Hair Induction by Cultured Mesenchymal Cells Using Sphere Formation Kazuo Kishi and Ruka Hayashi 11. Stereological Quantification of Cell Cycle Kinetics and Mobilization of Epithelial Stem Cells during Wound Healing Eduardo Martнnez-Martнnez, Eileen Uribe-Querol, Claudio I. Galvбn-Hernбndez, and Gabriel Gutiйrrez-Ospina 12. Culture of Dermal Papilla Cells from Ovine Wool Follicles: An In Vitro Model for Papilla Size-Determination Nicholas W. Rufaut, Allan J. Nixon, and Rodney D. Sinclair 13. Isolation and Fluorescence Activated Cell-Sorting of Mouse Keratinocytes Expressing β-Galactosidase Maria Kasper, Rune Toftgеrd, and Viljar Jaks 14. Ectopic Hair Growth from Transplanted Whisker Follicles on the Spinal Cord of Mice Wenluo Cao, Fang Liu, Yasuyuki Amoh, and Robert M. Hoffman
15. Long-Term Gelfoam(R) Histoculture of Hair-Shaft-Producing Mouse Whisker Follicles Wenluo Cao, Fang Liu, Yasuyuki Amoh, and Robert M. Hoffman 16. From Hair to Heart: Hair Follicle-Associated-Pluripotent (HAP) Stem Cells Differentiate to Beating Cardiac Muscle Cells Masateru Yashiro, Sumiyuki Mii, Ryoichi Aki, Yuko Hamada, Nobuko Arakawa, Katsumasa Kawahara, Robert M. Hoffman, and Yasuyuki Amoh 17.Highly Efficient Neural Differentiation of CD34 Positive Hair Follicle-Associated Pluripotent (HAP) Stem Cells Induced by Retinoic Acid and Serum Free Medium Mohsen Sagha and Nowruz Najafzadeh 18. Cryopreservation of Intact Hair Follicle Maintains Pluripotency of Nestin-Expressing (HAP) Stem Cells Satoshi Kajiura, Sumiyuki Mii, Ryoichi Aki, Yuko Hamada, Nobuko Arakawa, Katsumasa Kawahara, Lingna Li, Kensei Katsuoka, Robert M. Hoffman, and Yasuyuki Amoh
Автор: Muller Fabbri Название: Non-coding RNAs and Cancer ISBN: 146148443X ISBN-13(EAN): 9781461484431 Издательство: Springer Рейтинг: Цена: 23508.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This book provides essential information of the biogenesis, physiology and de-regulation of non-coding RNAs in cancer. It guides the reader to the most widely and effectively used bioinformatic approaches to the study of miRNAs.
Автор: Volker A. Erdmann; Jan Barciszewski Название: Non Coding RNAs in Plants ISBN: 3642269990 ISBN-13(EAN): 9783642269998 Издательство: Springer Рейтинг: Цена: 26120.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Discovered just 20 years ago, ncRNAs have been dubbed the `secret regulators` of cells. Only recently has their function in plants been investigated. In this book leading researchers relate recent discoveries about the role played by small ncRNA in plants.
Автор: Song Erwei Название: The Long and Short Non-Coding Rnas in Cancer Biology ISBN: 9811093679 ISBN-13(EAN): 9789811093678 Издательство: Springer Рейтинг: Цена: 20962.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Non-coding RNAs: New Players in Cancers.- The Working Modules of Long Non-coding RNAs in Cancer Cells.- Methods to Study Long Non-coding RNA Biology in Cancer.- Relationship between Non-coding RNA Dysregulation and Epigenetic Mechanisms in Cancer.- Non-coding RNAs in Growth and Death of Cancer Cells.- Non-coding RNAs in Cancer Cell Plasticity.- Non-coding RNAs in Regulation of Cancer Metabolic Reprogramming.- Non-coding RNAs in Tumor Angiogenesis.- Non-coding RNAs in Cancer Immunity.- Non-coding RNAs in Therapeutic Resistance of Cancer.- Non-coding RNAs Regulating Cancer Signaling Network.- Non-coding RNAs Regulating NFkB Signaling.- Non-coding RNAs Regulating p53 and c-Myc Signaling.- Viral Non-coding RNAs in Cancer Biology.- Non-coding RNAs in Cancer Diagnosis.- Therapeutic Potentials of Non-coding RNAs: Targeted Delivery of NcRNAs in Cancer Cells.
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