Adult Stem Cells: Methods and Protocols, Di Nardo Paolo, Dhingra Sanjiv, Singla Dinender K.
Автор: Zachariou Michael Название: Affinity Chromatography / Methods and Protocols ISBN: 1588296598 ISBN-13(EAN): 9781588296597 Издательство: Springer Рейтинг: Цена: 2000.00 р. Наличие на складе: Есть (2 шт.) Описание: Thirty-eight years after its introduction, affinity chromatography remains a key tool in th armory of separation techniques available to separation and interaction scientists. Expanded and updated from the first edition, this second edition volume aims to provide the beginner with the practical knowledge needed to develop affinity separations suitable for a variety of applications relevant to the post-genomic era.
Автор: Didenko Vladimir V. Название: In Situ Detection of DNA Damage / Methods and Protocols ISBN: 0896039528 ISBN-13(EAN): 9780896039520 Издательство: Springer Рейтинг: Цена: 5809.00 р. Наличие на складе: Есть (4 шт.) Описание: In In Situ Detection of DNA Damage: Methods and Protocols, Vladimir Didenko and a panel of experts describe all the major in situ techniques for studying DNA damage and apoptosis, and how approaches originally designed to label apoptotic cells can be used for DNA damage analysis (and vice versa). The readily reproducible techniques presented here include all those designed to detect specific single- and double-stranded DNA breaks in tissue sections while using polymerases, ligases, nucleases, and kinases. There are also novel instrumental techniques that use ultrasound for in vivo and in situ detection of apoptotic DNA damage, and in situ applications of flow and laser-scanning cytometry for analysis of DNA strand breaks and apoptosis. State-of-the-art and highly practical, In Situ Detection of DNA Damage: Methods and Protocols presents for the first time a complete set of enzymatic methods for determining DNA breaks, and will greatly facilitate the work of all those studying the in situ detection of DNA damage and the labeling of apoptotic cells.
1. Introduction to Hair-Follicle-Associated Pluripotent (HAP) Stem Cells Robert M. Hoffman 2. Nestin-Based Reporter Transgenic Mouse Lines John Mignone, Natalia Peunova, and Grigori Enikolopov 3. Discovery of HAP Stem Cells Lingna Li and Robert M. Hoffman 4. Peripheral-Nerve and Spinal-Cord Regeneration in Mice Using Hair Follicle-Associated-Pluripotent (HAP) Stem Cells Yasuyuki Amoh, Kensei Katsuoka, and Robert M. Hoffman 5. Construction of Tissue Engineered Nerve Conduits Seeded with Neurons Derived from Hair Follicle Neural Crest Stem Cells Fang Liu, Haiyan Lin, and Chuansen Zhang 6.Nestin-Expressing Hair Follicle-Associated Pluripotent (HAP) Stem Cells Promote Whisker Sensory-Nerve Growth in Long-Term 3D-Gelfoam Histoculture Sumiyuki Mii, Jennifer Duong, Yasunori Tome, Aisada Uchugonova, Fang Liu, Yasuyuki Amoh, Norimitsu Saito, Kensei Katsuoka, and Robert M. Hoffman 7. Isolation and Culture of Neural Crest Stem Cells from Human Hair Follicles Ruifeng Yang and Xiaowei Xu
8. Isolation of Mouse Hair Follicle Bulge Stem Cells and their Functional Analysis in a Reconstitution Assay Ying Zheng, Jen-Chih Hsieh, Julia Escandon, and George Cotsarelis 9.Hair Follicle Regeneration by Transplantation of a Bioengineered Hair Follicle Germ Katsunari Tezuka, Koh-ei Toyoshima, and Takashi Tsuji 10. Hair Induction by Cultured Mesenchymal Cells Using Sphere Formation Kazuo Kishi and Ruka Hayashi 11. Stereological Quantification of Cell Cycle Kinetics and Mobilization of Epithelial Stem Cells during Wound Healing Eduardo Martнnez-Martнnez, Eileen Uribe-Querol, Claudio I. Galvбn-Hernбndez, and Gabriel Gutiйrrez-Ospina 12. Culture of Dermal Papilla Cells from Ovine Wool Follicles: An In Vitro Model for Papilla Size-Determination Nicholas W. Rufaut, Allan J. Nixon, and Rodney D. Sinclair 13. Isolation and Fluorescence Activated Cell-Sorting of Mouse Keratinocytes Expressing β-Galactosidase Maria Kasper, Rune Toftgеrd, and Viljar Jaks 14. Ectopic Hair Growth from Transplanted Whisker Follicles on the Spinal Cord of Mice Wenluo Cao, Fang Liu, Yasuyuki Amoh, and Robert M. Hoffman
15. Long-Term Gelfoam(R) Histoculture of Hair-Shaft-Producing Mouse Whisker Follicles Wenluo Cao, Fang Liu, Yasuyuki Amoh, and Robert M. Hoffman 16. From Hair to Heart: Hair Follicle-Associated-Pluripotent (HAP) Stem Cells Differentiate to Beating Cardiac Muscle Cells Masateru Yashiro, Sumiyuki Mii, Ryoichi Aki, Yuko Hamada, Nobuko Arakawa, Katsumasa Kawahara, Robert M. Hoffman, and Yasuyuki Amoh 17.Highly Efficient Neural Differentiation of CD34 Positive Hair Follicle-Associated Pluripotent (HAP) Stem Cells Induced by Retinoic Acid and Serum Free Medium Mohsen Sagha and Nowruz Najafzadeh 18. Cryopreservation of Intact Hair Follicle Maintains Pluripotency of Nestin-Expressing (HAP) Stem Cells Satoshi Kajiura, Sumiyuki Mii, Ryoichi Aki, Yuko Hamada, Nobuko Arakawa, Katsumasa Kawahara, Lingna Li, Kensei Katsuoka, Robert M. Hoffman, and Yasuyuki Amoh
Автор: Perdiguero Eusebio, Cornelison Ddw Название: Muscle Stem Cells: Methods and Protocols ISBN: 1493982966 ISBN-13(EAN): 9781493982967 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume provides leading-edge protocols in the study of the molecular and cellular biology of muscle stem cells.
Автор: Conboy Irina M., Schaffer David V., Barcellos-Hoff Mary Helen Название: Protocols for Adult Stem Cells ISBN: 1493961047 ISBN-13(EAN): 9781493961047 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Although embryonic stem cells currently enjoy the public limelight and show great pr- ise for cell based medical therapies, it is the adult stem cells which are responsible for the body’s natural ability to fght disease, heal and recover, or fail and succumb to various maladies. The study of mammalian adult stem cells has surged recently, most likely from a maturation of stem cell studies in the classical developmental model organisms and in hematopoeisis. All the tissues of the body examined so far are generated and regenerated from stem cells, it has been an important frst step to adapt or devise new methods to identify and obtain these cells in quantity and purity for further study. Culture techniques have been optimized for managing the growth and differentiation of stem cells in vitro; as some stem cells are pluripotent, often the method is to guide the fate of such cells among the possible differentiation fates. Much of this work, and that in the classical model org- isms, has helped defne the aspects of the stem cell environment or niche that are crucial for both growth and differentiation, and these studies have moved in vivo at increasingly higher resolution. Importantly, the in vivo niche is a current target for bioengineering the matrix and signaling factors. Herein, we present methods for studying six types of mammalian stem cells, m- mary, neural, mesenchymal, endothelial, dendritic, and muscle.
Автор: Goswami Ritobrata Название: Th9 Cells: Methods and Protocols ISBN: 1493983261 ISBN-13(EAN): 9781493983261 Издательство: Springer Рейтинг: Цена: 19564.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: The techniques described in Th9 Cells: Methods and Protocols study the role of Th9 cells in different inflammatory disease models, including allergic inflammation model, parasite model, tumor model, and EAE and IBD model.
Автор: Turksen Kursad Название: Epidermal Cells: Methods and Protocols ISBN: 1071602500 ISBN-13(EAN): 9781071602508 Издательство: Springer Рейтинг: Цена: 18167.00 р. Наличие на складе: Поставка под заказ.
Описание: This fully updated edition brings together a new set of protocols to arm epidermal cell biologists with tools and approaches to continue the quest to uncover the intricacies and regulatory mechanisms underlying epidermal cell development and function.
Автор: Turksen Kursad Название: Stem Cell Heterogeneity: Methods and Protocols ISBN: 1493982346 ISBN-13(EAN): 9781493982349 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Given the variety of studies and data that have suggested the existence of heterogeneous populations or subpopulations of stem cells, this detailed volume examines different aspects of stem cell heterogeneity.
