Epidermal Cells: Methods and Protocols, Turksen Kursad
Автор: Didenko Vladimir V. Название: In Situ Detection of DNA Damage / Methods and Protocols ISBN: 0896039528 ISBN-13(EAN): 9780896039520 Издательство: Springer Рейтинг: Цена: 5809.00 р. Наличие на складе: Есть (4 шт.) Описание: In In Situ Detection of DNA Damage: Methods and Protocols, Vladimir Didenko and a panel of experts describe all the major in situ techniques for studying DNA damage and apoptosis, and how approaches originally designed to label apoptotic cells can be used for DNA damage analysis (and vice versa). The readily reproducible techniques presented here include all those designed to detect specific single- and double-stranded DNA breaks in tissue sections while using polymerases, ligases, nucleases, and kinases. There are also novel instrumental techniques that use ultrasound for in vivo and in situ detection of apoptotic DNA damage, and in situ applications of flow and laser-scanning cytometry for analysis of DNA strand breaks and apoptosis. State-of-the-art and highly practical, In Situ Detection of DNA Damage: Methods and Protocols presents for the first time a complete set of enzymatic methods for determining DNA breaks, and will greatly facilitate the work of all those studying the in situ detection of DNA damage and the labeling of apoptotic cells.
Автор: Zachariou Michael Название: Affinity Chromatography / Methods and Protocols ISBN: 1588296598 ISBN-13(EAN): 9781588296597 Издательство: Springer Рейтинг: Цена: 2000.00 р. Наличие на складе: Есть (2 шт.) Описание: Thirty-eight years after its introduction, affinity chromatography remains a key tool in th armory of separation techniques available to separation and interaction scientists. Expanded and updated from the first edition, this second edition volume aims to provide the beginner with the practical knowledge needed to develop affinity separations suitable for a variety of applications relevant to the post-genomic era.
Автор: Goswami Ritobrata Название: Th9 Cells: Methods and Protocols ISBN: 1493983261 ISBN-13(EAN): 9781493983261 Издательство: Springer Рейтинг: Цена: 19564.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: The techniques described in Th9 Cells: Methods and Protocols study the role of Th9 cells in different inflammatory disease models, including allergic inflammation model, parasite model, tumor model, and EAE and IBD model.
Автор: Kursad Turksen Название: Epidermal Cells ISBN: 1493955780 ISBN-13(EAN): 9781493955787 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Reflecting over three decades of advances, Epidermal Cells: Methods and Protocols, Third Edition underscores these advances in our understanding of epidermal biology with updated and entirely new protocols that compliment and extend the earlier edition.
Автор: Perez-Moreno, Mirna Название: Epidermal Stem Cell Niche,3 ISBN: 0128184469 ISBN-13(EAN): 9780128184462 Издательство: Elsevier Science Рейтинг: Цена: 21054.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Dit boek biedt alle basiskennis die je nodig hebt om het vak projectmanagement te leren kennen. Het is een toegankelijk boek voor startende projectmanagers. Het boek is gebaseerd op het competentieprofiel voor IPMA-D van de International Project Management Association: IPMA. In dat profiel wordt evenwichtig aandacht besteed aan het vak projectmanagement, aan de omgevingen waarbinnen dat vak wordt uitgevoerd en aan persoonlijk leiderschap.In het boek worden deze drie kenmerkende elementen van IPMA op een toegankelijke manier met elkaar vervlochten. De schrijvers hebben daarbij gekozen voor een logische opzet, waarbij ze je in vijf delen meenemen door de levensduur van een project. Na een orientatie op `wat projecten zijn`, staat in de voorgeschiedenis het verkennen van de projectomgeving centraal. In de voorbereidingsfase het contact tussen de projectmanager en de opdrachtgever. In de definitiefase wordt het project opgezet en in de uitvoeringsfasen wordt het project beheerst gerealiseerd. In de gebruiksfase wordt beoordeeld of met het project beoogde doelen worden gerealiseerd. Een casus verbindt de delen en ieder deel start met hoofdstukken over persoonlijk leiderschap.Het boek behandelt alle eindtermen van het competentieprofiel op IPMA-D niveau en biedt een uitstekende basis voor het behalen van dit examen. De eisen voor dit examen vind je op: www.ipmacertificeren.nl.Voor studenten in HBO en WO biedt het boek, mede vanwege de vele voorbeelden en praktische checklists, een mooi uitgangspunt om alle basisprincipes van projectmanagement samenhangend te bestuderen en vooral ook om er mee aan de slag te gaan. Op de website www.PM4you.nl wordt extra ondersteuningsmateriaal aangeboden.
Автор: Di Nardo Paolo, Dhingra Sanjiv, Singla Dinender K. Название: Adult Stem Cells: Methods and Protocols ISBN: 1493982931 ISBN-13(EAN): 9781493982936 Издательство: Springer Рейтинг: Цена: 16769.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
Preface... Table of Contents... Contributing Authors...
1. A Simple Protocol to Isolate, Characterize and Expand Dental Pulp Stem Cells Federica Di Scipio, Andrea Elio Sprio, Maria Elisabetta Carere, Zhiqian Yang, and Giovanni Nicolao Berta 2. Derivation of Mesenchymal Stem cells from Embryonic Stem Cells: A Non-Variable and Inexhaustive Source of Adult Stem Cells Glen Lester Sequiera, Niketa Sareen, Ejlal Abu Al-Rub, and Sanjiv Dhingra 3. Compact Bone-Derived Multipotent Mesenchymal Stromal Cells (MSCs) for the Treatment of Sjogren's-Like Disease in NOD Mice Ghada Abu Elghanam, Younan Liu, Saeed Khalili, Dongdong Fang, and Simon D.Tran 4. Manipulating the Proliferative Potential of Cardiomyocytes by Gene Transfer Giulia Prosdocimo and Mauro Giacca 5. Assessment of Energy Metabolic Changes in Adipose Tissue-Derived Stem Cells Ghazaleh Hajmousa and Martin C. Harmsen 6. Isolation and In Vitro Characterization of Epidermal Stem Cells Kasper S. Moestrup, Marianne S. Andersen, and Kim B. Jensen 7. Endothelial Progenitor Cells: Procedure for Cell Isolation and Characterization Garikipati V.N. Srikanth, Zhongjian Cheng, and Raj Kishore 8. Therapeutic Application of Placental Mesenchymal Stem Cells Reprogrammed Neurospheres in Spinal Cord Injury of SCID Vikram Sabapathy, Franklin Jebaraj Herbert, and Sanjay Kumar 9. Therapeutic Application of Human Wharton Jelly Mesenchymal Stem Cells in Skin Injury of SCID Vikram Sabapathy, Balasubramanian Sundaram, and Sanjay Kumar 10. Isolation, Characterization, and Expansion of Cancer Stem Cells Luke A. Torre-Healy, Artem Berezovsky, and Justin D. Lathia 11. Cardiac Progenitor Cell Isolation from Human Auricles Paolo Di Nardo and Francesca Pagliari 12. Isolation and Culture of Satellite Cells from Mouse Skeletal Muscle Antonio Musarт and Silvia Carosio 13. Isolation of Stromal Stem Cells from Adipose Tissue Maria Prat, Francesca Oltolina, Silvia Antonini, and Andrea Zamperone 14. Human Induced Pluripotent Stem Cell-Derived Mesencymal Stem Cells as an Individual-Specific and Renewable Source of Adult Stem Cells15. A Simplified and Systematic Method to Isolate, Culture and Characterize Multiple Types of Human Dental Stem Cells from a Single Tooth Mohammed Bakkar, Younan Liu, Dongdong Fang, Camille Stegen, Xinyun Su, Murali Ramamoorthi, Li-Chieh Lin, Takako Kawasaki, Nicholas Makhoul, Huan Pham, Yoshinori Sumita, and Simon D. Tran 16. Measurement of Autophagy by Flow Cytometry Silvia Zappavigna, Angela Lombardi, Gabriella Misso, Anna Grimaldi, and Michele Caraglia 17. Echocardiography-Guided Intramyocardial Injection Method in a Murine Model Kay Maeda, Rick Seymour, Marc Ruel, and Erik J. Suuronen 18. Noninvasive Assessment of Cell Fate and Biology in Transplanted Mesenchymal Stem Cells Federico Franchi and Martin Rodriguez-Porcel 19. Methods for Long Term Storage of Murine Bone Marrow Derived Mesenchymal Stem Cells Niketa Sareen, Ejlal Abu-El-Rub, Glen Lester Sequiera, Meenal Moudgil, and Sanjiv Dhingra 20. Therapeutic Application of Adult Stem Cells in the Heart Taylor A. Johnson and Dinender K. Singla 21. Patient-Derived and Intra-Operatively Formed Biomaterial for Tissue Engineering Shalmli U. Joshi, Rares O. Barbu, Melissa Carr-Reynolds, Brian Barnes, and Syam P. Nukavarapu 22. Three-Dimensional Printed Scaffolds with Multipotent Mesenchymal Stromal Cells for Rabbit Mandibular Reconstruction and Engineering Dongdong Fang, Michael Roskies, Mohamed-Nur Abdallah, Mohammed Bakkar, Jack Jordan, Li-Chieh Lin, Faleh Tamimi, and Simon D. Tran 23. Optimal Enviro
Автор: Sarkar Sibaji Название: Histone Deacetylases: Methods and Protocols ISBN: 1493981110 ISBN-13(EAN): 9781493981113 Издательство: Springer Рейтинг: Цена: 6986.00 р. Наличие на складе: Поставка под заказ.
Описание: This volume provides different methodologies for all classes of histone deacetylases, which includes detailed procedures on Class I and II histone deacetylase inhibitors, SIRT inhibitors, and bromodomain inhibitors.
5. High-Efficiency Transfection of Glioblastoma Cells and a Simple Spheroid Migration Assay
Carsten Hagemann, Diana Amend, Almuth F. Kessler, Thomas Linsenmann, Ralf-Ingo Ernestus, and Mario Lцhr
6. Construction and Application of Random dsRNA Interference Library for Functional Genetic Screens in Embryonic Stem Cells
Xiaoxing Cheng and Rui Jian
7. Efficient Depletion of Essential Gene Products for Loss-of-Function Studies in Embryonic Stem Cells
Soizik Berlivet, Isabelle Hmitou, Hйlиne Picaud, and Matthieu Gйrard
8. Regulation and/or Repression of Cholinergic Differentiation of Murine Embryonic Stem Cells Using RNAi Directed Against Transcription Factor L3/Lhx8
Takayuki Manabe and Akio Wanaka
9. A Recessive Genetic Screen for Components of the RNA Interference Pathway Performed in Mouse Embryonic Stem Cells
Melanie I. Trombly and Xiaozhong Wang
10. RNAi Knockdown of Ape1 Gene in the Differentiation of Mouse Embryonic Stem Cells
Gang-Ming Zou, Jieqing Yu, Cynthia LeBron, and Yumei Fu
11. An Efficient Transfection Method for Differentiation and Cell Proliferation of Mouse Embryonic Stem Cells
Jun-Yang Liou, Bor-Sheng Ko, and Tzu-Ching Chang
12. Implanting Glioblastoma Spheroids into Rat Brains and Monitoring Tumor Growth by MRI Volumetry
Mario Lцhr, Thomas Linsenmann, Anna Jawork, Almuth F. Kessler, Nils Timmermann, Gyцrgy A. Homola, Ralf-Ingo Ernestus, and Carsten Hagemann
13. RNAi-Based Techniques for the Analysis of Gene Function in Drosophila Germline Stem Cells
Amelia J. Helms, Danielle S. Finger, Victoria L. Hardy, and Elizabeth T. Ables
14. In Vivo RNAi in the Drosophila Follicular Epithelium: Analysis of Stem Cell Maintenance, Proliferation, and Differentiation
Veit Riechmann
15. A Phenotype-Based RNAi Screening for Ras-ERK/MAPK Signaling-Associated Stem Cell Regulators in C. elegans
Myon-Hee Lee and Dong Suk Yoon
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Автор: Amarnath Shoba Название: Innate Lymphoid Cells: Methods and Protocols ISBN: 107160337X ISBN-13(EAN): 9781071603376 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Поставка под заказ.
Автор: Stephen Misener; Stephen A. Krawetz Название: Bioinformatics Methods and Protocols ISBN: 1617371564 ISBN-13(EAN): 9781617371561 Издательство: Springer Рейтинг: Цена: 23058.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: We divided Bioinformatics Methods and Protocols into five parts, including a thorough survey of the basic sequence analysis software packages that are available at most institutions, as well as the design and implemen- tion of an essential introductory Bioinformatics course.
Автор: Costa Cristina, Mбсez Rafael Название: Xenotransplantation: Methods and Protocols ISBN: 1493958690 ISBN-13(EAN): 9781493958696 Издательство: Springer Рейтинг: Цена: 20263.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: In this book, experts study characterizations of xenogeneic interactions at the cellular and molecular levels and describe the use of relevant small-animal and pig-to-primate models. It also covers related ethical and legal considerations.
1. Introduction to Hair-Follicle-Associated Pluripotent (HAP) Stem Cells Robert M. Hoffman 2. Nestin-Based Reporter Transgenic Mouse Lines John Mignone, Natalia Peunova, and Grigori Enikolopov 3. Discovery of HAP Stem Cells Lingna Li and Robert M. Hoffman 4. Peripheral-Nerve and Spinal-Cord Regeneration in Mice Using Hair Follicle-Associated-Pluripotent (HAP) Stem Cells Yasuyuki Amoh, Kensei Katsuoka, and Robert M. Hoffman 5. Construction of Tissue Engineered Nerve Conduits Seeded with Neurons Derived from Hair Follicle Neural Crest Stem Cells Fang Liu, Haiyan Lin, and Chuansen Zhang 6.Nestin-Expressing Hair Follicle-Associated Pluripotent (HAP) Stem Cells Promote Whisker Sensory-Nerve Growth in Long-Term 3D-Gelfoam Histoculture Sumiyuki Mii, Jennifer Duong, Yasunori Tome, Aisada Uchugonova, Fang Liu, Yasuyuki Amoh, Norimitsu Saito, Kensei Katsuoka, and Robert M. Hoffman 7. Isolation and Culture of Neural Crest Stem Cells from Human Hair Follicles Ruifeng Yang and Xiaowei Xu
8. Isolation of Mouse Hair Follicle Bulge Stem Cells and their Functional Analysis in a Reconstitution Assay Ying Zheng, Jen-Chih Hsieh, Julia Escandon, and George Cotsarelis 9.Hair Follicle Regeneration by Transplantation of a Bioengineered Hair Follicle Germ Katsunari Tezuka, Koh-ei Toyoshima, and Takashi Tsuji 10. Hair Induction by Cultured Mesenchymal Cells Using Sphere Formation Kazuo Kishi and Ruka Hayashi 11. Stereological Quantification of Cell Cycle Kinetics and Mobilization of Epithelial Stem Cells during Wound Healing Eduardo Martнnez-Martнnez, Eileen Uribe-Querol, Claudio I. Galvбn-Hernбndez, and Gabriel Gutiйrrez-Ospina 12. Culture of Dermal Papilla Cells from Ovine Wool Follicles: An In Vitro Model for Papilla Size-Determination Nicholas W. Rufaut, Allan J. Nixon, and Rodney D. Sinclair 13. Isolation and Fluorescence Activated Cell-Sorting of Mouse Keratinocytes Expressing β-Galactosidase Maria Kasper, Rune Toftgеrd, and Viljar Jaks 14. Ectopic Hair Growth from Transplanted Whisker Follicles on the Spinal Cord of Mice Wenluo Cao, Fang Liu, Yasuyuki Amoh, and Robert M. Hoffman
15. Long-Term Gelfoam(R) Histoculture of Hair-Shaft-Producing Mouse Whisker Follicles Wenluo Cao, Fang Liu, Yasuyuki Amoh, and Robert M. Hoffman 16. From Hair to Heart: Hair Follicle-Associated-Pluripotent (HAP) Stem Cells Differentiate to Beating Cardiac Muscle Cells Masateru Yashiro, Sumiyuki Mii, Ryoichi Aki, Yuko Hamada, Nobuko Arakawa, Katsumasa Kawahara, Robert M. Hoffman, and Yasuyuki Amoh 17.Highly Efficient Neural Differentiation of CD34 Positive Hair Follicle-Associated Pluripotent (HAP) Stem Cells Induced by Retinoic Acid and Serum Free Medium Mohsen Sagha and Nowruz Najafzadeh 18. Cryopreservation of Intact Hair Follicle Maintains Pluripotency of Nestin-Expressing (HAP) Stem Cells Satoshi Kajiura, Sumiyuki Mii, Ryoichi Aki, Yuko Hamada, Nobuko Arakawa, Katsumasa Kawahara, Lingna Li, Kensei Katsuoka, Robert M. Hoffman, and Yasuyuki Amoh
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