Th9 Cells: Methods and Protocols, Goswami Ritobrata
Автор: Didenko Vladimir V. Название: In Situ Detection of DNA Damage / Methods and Protocols ISBN: 0896039528 ISBN-13(EAN): 9780896039520 Издательство: Springer Рейтинг: Цена: 5809.00 р. Наличие на складе: Есть (4 шт.) Описание: In In Situ Detection of DNA Damage: Methods and Protocols, Vladimir Didenko and a panel of experts describe all the major in situ techniques for studying DNA damage and apoptosis, and how approaches originally designed to label apoptotic cells can be used for DNA damage analysis (and vice versa). The readily reproducible techniques presented here include all those designed to detect specific single- and double-stranded DNA breaks in tissue sections while using polymerases, ligases, nucleases, and kinases. There are also novel instrumental techniques that use ultrasound for in vivo and in situ detection of apoptotic DNA damage, and in situ applications of flow and laser-scanning cytometry for analysis of DNA strand breaks and apoptosis. State-of-the-art and highly practical, In Situ Detection of DNA Damage: Methods and Protocols presents for the first time a complete set of enzymatic methods for determining DNA breaks, and will greatly facilitate the work of all those studying the in situ detection of DNA damage and the labeling of apoptotic cells.
Автор: Zachariou Michael Название: Affinity Chromatography / Methods and Protocols ISBN: 1588296598 ISBN-13(EAN): 9781588296597 Издательство: Springer Рейтинг: Цена: 2000.00 р. Наличие на складе: Есть (2 шт.) Описание: Thirty-eight years after its introduction, affinity chromatography remains a key tool in th armory of separation techniques available to separation and interaction scientists. Expanded and updated from the first edition, this second edition volume aims to provide the beginner with the practical knowledge needed to develop affinity separations suitable for a variety of applications relevant to the post-genomic era.
Автор: Jin Tian, Hereld Dale Название: Chemotaxis: Methods and Protocols ISBN: 1493980599 ISBN-13(EAN): 9781493980598 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This second edition expands on the first edition with new chapters describing methods for studyingcell movement, molecular components involved in chemotaxis, spatiotemporaldynamics of signaling components, and quantitative modeling, as well as severalupdated chapters from the first edition.
Автор: Higgins Paul J. Название: Transcription Factors: Methods and Protocols ISBN: 1493957155 ISBN-13(EAN): 9781493957156 Издательство: Springer Рейтинг: Цена: 20962.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Here, leading researchers describe the latest techniques for investigating the mechanisms underlying transcription factor nuclear-cytoplasmic trafficking, as well as the functional impact of post-translational modifications on transcription factor function.
Автор: Ritobrata Goswami Название: Th9 Cells ISBN: 1493968769 ISBN-13(EAN): 9781493968763 Издательство: Springer Рейтинг: Цена: 19564.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: The techniques described in Th9 Cells: Methods and Protocols study the role of Th9 cells in different inflammatory disease models, including allergic inflammation model, parasite model, tumor model, and EAE and IBD model.
5. High-Efficiency Transfection of Glioblastoma Cells and a Simple Spheroid Migration Assay
Carsten Hagemann, Diana Amend, Almuth F. Kessler, Thomas Linsenmann, Ralf-Ingo Ernestus, and Mario Lцhr
6. Construction and Application of Random dsRNA Interference Library for Functional Genetic Screens in Embryonic Stem Cells
Xiaoxing Cheng and Rui Jian
7. Efficient Depletion of Essential Gene Products for Loss-of-Function Studies in Embryonic Stem Cells
Soizik Berlivet, Isabelle Hmitou, Hйlиne Picaud, and Matthieu Gйrard
8. Regulation and/or Repression of Cholinergic Differentiation of Murine Embryonic Stem Cells Using RNAi Directed Against Transcription Factor L3/Lhx8
Takayuki Manabe and Akio Wanaka
9. A Recessive Genetic Screen for Components of the RNA Interference Pathway Performed in Mouse Embryonic Stem Cells
Melanie I. Trombly and Xiaozhong Wang
10. RNAi Knockdown of Ape1 Gene in the Differentiation of Mouse Embryonic Stem Cells
Gang-Ming Zou, Jieqing Yu, Cynthia LeBron, and Yumei Fu
11. An Efficient Transfection Method for Differentiation and Cell Proliferation of Mouse Embryonic Stem Cells
Jun-Yang Liou, Bor-Sheng Ko, and Tzu-Ching Chang
12. Implanting Glioblastoma Spheroids into Rat Brains and Monitoring Tumor Growth by MRI Volumetry
Mario Lцhr, Thomas Linsenmann, Anna Jawork, Almuth F. Kessler, Nils Timmermann, Gyцrgy A. Homola, Ralf-Ingo Ernestus, and Carsten Hagemann
13. RNAi-Based Techniques for the Analysis of Gene Function in Drosophila Germline Stem Cells
Amelia J. Helms, Danielle S. Finger, Victoria L. Hardy, and Elizabeth T. Ables
14. In Vivo RNAi in the Drosophila Follicular Epithelium: Analysis of Stem Cell Maintenance, Proliferation, and Differentiation
Veit Riechmann
15. A Phenotype-Based RNAi Screening for Ras-ERK/MAPK Signaling-Associated Stem Cell Regulators in C. elegans
Myon-Hee Lee and Dong Suk Yoon
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Автор: Di Nardo Paolo, Dhingra Sanjiv, Singla Dinender K. Название: Adult Stem Cells: Methods and Protocols ISBN: 1493982931 ISBN-13(EAN): 9781493982936 Издательство: Springer Рейтинг: Цена: 16769.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
Preface... Table of Contents... Contributing Authors...
1. A Simple Protocol to Isolate, Characterize and Expand Dental Pulp Stem Cells Federica Di Scipio, Andrea Elio Sprio, Maria Elisabetta Carere, Zhiqian Yang, and Giovanni Nicolao Berta 2. Derivation of Mesenchymal Stem cells from Embryonic Stem Cells: A Non-Variable and Inexhaustive Source of Adult Stem Cells Glen Lester Sequiera, Niketa Sareen, Ejlal Abu Al-Rub, and Sanjiv Dhingra 3. Compact Bone-Derived Multipotent Mesenchymal Stromal Cells (MSCs) for the Treatment of Sjogren's-Like Disease in NOD Mice Ghada Abu Elghanam, Younan Liu, Saeed Khalili, Dongdong Fang, and Simon D.Tran 4. Manipulating the Proliferative Potential of Cardiomyocytes by Gene Transfer Giulia Prosdocimo and Mauro Giacca 5. Assessment of Energy Metabolic Changes in Adipose Tissue-Derived Stem Cells Ghazaleh Hajmousa and Martin C. Harmsen 6. Isolation and In Vitro Characterization of Epidermal Stem Cells Kasper S. Moestrup, Marianne S. Andersen, and Kim B. Jensen 7. Endothelial Progenitor Cells: Procedure for Cell Isolation and Characterization Garikipati V.N. Srikanth, Zhongjian Cheng, and Raj Kishore 8. Therapeutic Application of Placental Mesenchymal Stem Cells Reprogrammed Neurospheres in Spinal Cord Injury of SCID Vikram Sabapathy, Franklin Jebaraj Herbert, and Sanjay Kumar 9. Therapeutic Application of Human Wharton Jelly Mesenchymal Stem Cells in Skin Injury of SCID Vikram Sabapathy, Balasubramanian Sundaram, and Sanjay Kumar 10. Isolation, Characterization, and Expansion of Cancer Stem Cells Luke A. Torre-Healy, Artem Berezovsky, and Justin D. Lathia 11. Cardiac Progenitor Cell Isolation from Human Auricles Paolo Di Nardo and Francesca Pagliari 12. Isolation and Culture of Satellite Cells from Mouse Skeletal Muscle Antonio Musarт and Silvia Carosio 13. Isolation of Stromal Stem Cells from Adipose Tissue Maria Prat, Francesca Oltolina, Silvia Antonini, and Andrea Zamperone 14. Human Induced Pluripotent Stem Cell-Derived Mesencymal Stem Cells as an Individual-Specific and Renewable Source of Adult Stem Cells15. A Simplified and Systematic Method to Isolate, Culture and Characterize Multiple Types of Human Dental Stem Cells from a Single Tooth Mohammed Bakkar, Younan Liu, Dongdong Fang, Camille Stegen, Xinyun Su, Murali Ramamoorthi, Li-Chieh Lin, Takako Kawasaki, Nicholas Makhoul, Huan Pham, Yoshinori Sumita, and Simon D. Tran 16. Measurement of Autophagy by Flow Cytometry Silvia Zappavigna, Angela Lombardi, Gabriella Misso, Anna Grimaldi, and Michele Caraglia 17. Echocardiography-Guided Intramyocardial Injection Method in a Murine Model Kay Maeda, Rick Seymour, Marc Ruel, and Erik J. Suuronen 18. Noninvasive Assessment of Cell Fate and Biology in Transplanted Mesenchymal Stem Cells Federico Franchi and Martin Rodriguez-Porcel 19. Methods for Long Term Storage of Murine Bone Marrow Derived Mesenchymal Stem Cells Niketa Sareen, Ejlal Abu-El-Rub, Glen Lester Sequiera, Meenal Moudgil, and Sanjiv Dhingra 20. Therapeutic Application of Adult Stem Cells in the Heart Taylor A. Johnson and Dinender K. Singla 21. Patient-Derived and Intra-Operatively Formed Biomaterial for Tissue Engineering Shalmli U. Joshi, Rares O. Barbu, Melissa Carr-Reynolds, Brian Barnes, and Syam P. Nukavarapu 22. Three-Dimensional Printed Scaffolds with Multipotent Mesenchymal Stromal Cells for Rabbit Mandibular Reconstruction and Engineering Dongdong Fang, Michael Roskies, Mohamed-Nur Abdallah, Mohammed Bakkar, Jack Jordan, Li-Chieh Lin, Faleh Tamimi, and Simon D. Tran 23. Optimal Enviro
Автор: Amarnath Shoba Название: Innate Lymphoid Cells: Methods and Protocols ISBN: 107160337X ISBN-13(EAN): 9781071603376 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Поставка под заказ.
Автор: Schrader Michael Название: Peroxisomes: Methods and Protocols ISBN: 149398344X ISBN-13(EAN): 9781493983445 Издательство: Springer Рейтинг: Цена: 16070.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
1. Isolation of Peroxisomes from Rat Liver and Cultured Hepatoma Cells by Density Gradient Centrifugation
Andreas Manner and Markus Islinger
2. Isolation of Peroxisomes from Mouse Brain Using a Continuous Nycodenz Gradient: A Comparison to the Isolation of Liver and Kidney Peroxisomes
Miriam J. Schцnenberger and Werner J. Kovacs
3. Determining the Topology of Peroxisomal Proteins Using Protease Protection Assays
Tвnia Francisco, Ana F. Dias, Ana G. Pedrosa, Clбudia P. Grou, Tony A. Rodrigues, and Jorge E. Azevedo
4. Isolation of Native Soluble and Membrane-Bound Protein Complexes from Yeast Saccharomyces Cerevisiae
Tobias Hansen, Anna Chan, Thomas Schrцter, Daniel Schwerter, Wolfgang Girzalsky, and Ralf Erdmann
5. Method for Measurement of Peroxisomal Very Long-Chain Fatty Acid Beta-Oxidation and de novo C26:0 Synthesis Activity in Living Cells Using Stable-Isotope Labelled Docosanoic Acid
Malu-Clair van de Beek, Inge M.E. Dijkstra, and Stephan Kemp
6. Analysis of Plasmalogen Synthesis in Cultured Cells
Masanori Honsho and Yukio Fujiki
7. Transfection of Primary Human Skin Fibroblasts for Peroxisomal Studies
Janet Koster and Hans R. Waterham
8. siRNA-Mediated Silencing of Peroxisomal Genes in Mammalian Cells
Tina A. Schrader and Michael Schrader
9. Dual Reporter Systems for the Analysis of Translational Readthrough in Mammals
Julia Hofhuis, Severin Dieterle, Rosemol George, Fabian Schueren, and Sven Thoms
10. Cytochemical Detection of Peroxisomes in Light and Electron Microscopy with 3,3'-diaminobenzidine
H. Dariush Fahimi
11. Cryo-Immuno Electron Microscopy of Peroxisomal Marker Proteins
Karina Mildner and Dagmar Zeuschner
12. Detection and Immunolabelling of Peroxisomal Proteins
Tina Schrader, Markus Islinger, and Michael Schrader
13. Labelling of Peroxisomes for Live Cell Imaging in the Filamentous Fungus Ustilago Maydis
Sofia C. Guimaraes, Sreedhar Kilaru, Michael Schrader, and Martin Schuster
14. Quantitative Monitoring of Subcellular Redox Dynamics in Living Mammalian Cells Using RoGFP2-based Probes
Celien Lismont, Paul A. Walton, and Marc Fransen
15. KillerRed as a Tool to Study the Cellular Responses to Peroxisome-Derived Oxidative Stress
Marc Fransen and Chantal Brees
16. Determination of Peroxisomal pH in Living Mammalian Cells Using pHRed
Luis F. Godinho, and Michael Schrader
17. In Cellulo Approaches to Study Peroxisomal Protein Import - Yeast Immunofluorescence Microscopy
Tobias Hansen, Wolfgang Girzalsky, and Ralf Erdmann
18. Blue Native Page: Applications to Study Peroxisome Biogenesis
Kanji Okumoto, Shigehiko Tamura, and Yukio Fujiki
19. In Vitro PMP Import Analysis Using Cell-Free Synthesized PMP and Isolated Peroxisomes
Yuqiong Liu, Masanori Honsho, and Yukio Fujiki
20. Peroxisomal Membrane and Matrix Protein Import Using a Semi-Intact Mammalian Cell System
Kanji Okumoto, Masanori Honsho, Yuqiong Liu, and Yukio Fujiki
21. The Use of Glycosylation Tags as Reporters for Protein Entry into the Endoplasmic Reticulum in Yeast and Mammalian Cells
Judith Buentzel, and Sven Thoms
22. Detection of Ubiquitinated Peroxisomal Proteins in Yeast
Natasha Danda and Chris Williams
23. Assessing Pexophagy in Mammalian Cells
Shun-ichi Yamashita and Yukio Fujiki
24. Experimental Systems to Study Yeast Pexophagy
Shun-ichi Yamashita, Masahide O
Автор: Chang Paul, Ohi Ryoma Название: The Mitotic Spindle: Methods and Protocols ISBN: 1493980726 ISBN-13(EAN): 9781493980727 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
Part I Methods Focused on Cytology 1: Using Fluorescence Microscopy to Study MitosisSai K. Balchand, Barbara J. Mann, and Patricia Wadsworth 2: Using Photoactivatable GFP to Study Microtubule Dynamics and Chromosome Segregation Bin He and Daniela Cimini
Part II Methods Focused on Microtubules and the Mitotic Spindle
3: Purification and Fluorescent Labeling of Tubulin from Xenopus Laevis Egg Extracts Aaron C. Groen and Timothy J. Mitchison 4: Measuring the Effects of Microtubule-Associated Proteins on Microtubule Dynamics In Vitro Marija Zanic
5: Imaging and Quantifying the Dynamics of γ-Tubulin at Microtubule Minus Ends in Mitotic Spindles Nicolas Lecland and Jens Lьders 6: Visualizing and Analyzing Branching Microtubule Nucleation Using Meiotic Xenopus Egg Extracts and TIRF Microscopy Matthew King and Sabine Petry 7: Encapsulation of Xenopus Egg and Embryo Extract Spindle Assembly Reactions in Synthetic Cell-Like Compartments with Tunable Size Matthew C. Good Part III Methods Focused on Kinetochores and the Kinetochore-Microtubule Interface 8: In Vitro Kinetochore Assembly Matthew D. D. Miell and Aaron F. Straight 9: Biochemical and Structural Analysis of Kinetochore Histone-Fold Complexes Tatsuya Nishino and Tatsuo Fukagawa 10: Measuring Kinetochore-Microtubule Attachment Stability in Cultured Cells Keith F. DeLuca, Jacob A. Herman, and Jennifer G. DeLuca 11: Studying Kinetochores In Vivo Using FLIM-FRET Tae Yeon Yoo and Daniel J. Needleman Section IV Methods Focused on the Spindle Pole 12: Purification of Fluorescently Labeled Saccharomyces Cerevisiae Spindle Pole Bodies Kimberly K. Fong, Beth Graczyk, and Trisha N. Davis 13: A Cell-Free System for Real-Time Analyses of Centriole Disengagement and Centriole to Centrosome Conversion Rajesh Kumar Soni and Meng-Fu Bryan Tsou 14: Assays to Study Mitotic Centrosome and Spindle Pole Assembly and Regulation Vladimir Joukov, Johannes C. Walter, and Arcangela De Nicolo Part V Methods Focused on the Cellular Functions of Microtubule Motor Proteins 15: Analyzing Spindle Positioning Dynamics in Cultured Cells Tomomi Kiyomitsu 16: Quantification of Mitotic Chromosome Alignment Cindy Fonseca and Jason Stumpff 17: Imaging Mitosis in the Moss Physcomitrella Patens Moй Yamada, Tomohiro Miki, and Gohta Goshima 18: Small Molecule Approach to Study the Function of Mitotic Kinesins Naowras Al-Obaidi, Johanna Kastl, and Thomas U. Mayer Part VI Novel Approaches to Study Spindle Function and Regulation 19: Identification and Characterization of Mitotic Spindle-Localized Transcripts Amy B. Emerman, Ashwini Jambhekar, and Michael D. Blower 20: Probing Mitosis by Manipulating the Interactions of Mitotic Regulator Proteins Using Rapamycin-Inducible Dimerization Edward R. Ballister and Michael A. Lampson 21: Studying Kinetochore Kinases Adrian T. Saurin and Geert J.P.L. Kops 22: Engineering and Functional Analysis of Mitotic Kinases through Chemical Genetics Mathew J.K. Jones and Prasad V. Jallepalli Part VII The Mitotic Spindle and Cancer 23: Using Cell Culture Models of Centrosome Amplification to Study Centrosome Clustering in Cancer Mijung Kwon
Автор: Duque Paula Название: Environmental Responses in Plants: Methods and Protocols ISBN: 1493980297 ISBN-13(EAN): 9781493980291 Издательство: Springer Рейтинг: Цена: 16070.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
PaPart I Tropisms
1.11. Hydrotropism: Analysis of the Root Response to a Moisture Gradient Regina Antoni, Daniela Dietrich, Malcolm J. Bennett, and Pedro L. Rodriguez
2. Assessing Gravitropic Responses in Arabidopsis Richard Barker, Benjamin Cox, Logan Silber, Arah Sangari, Amir Assadi, and Patrick Masson
3. Physiological Analysis of Phototropic Responses in Arabidopsis Mathias Zeidler
4. Automatic Chloroplast Movement Analysis Henrik Johansson and Mathias Zeidler
5. Micro
scopic and Biochemical Visualization of Auxins in Plant Tissues Joshua J. Blakeslee and Angus S. Murphy
6. Immunolocalization of PIN and ABCB Transporters in Plants Nicola Carraro and Wendy A. Peer
Part II Photoperiodism and Circasian Rhythms
7.Analysis of Circadian Leaf Movements Niels A. Mьller and Josй M. Jimйmez-Gуmez
8. Sample Preparation of Arabidopsis Thaliana Shoot Apices for Expression Studies of Photoperiod-Induced Genes Fernando Andrйs, Stefano Torti, Coral Vincent, and George Coupland
9. A Luciferase-Based Assay to Test Whether Gene Expression Responses to Environmental Inputs are
Temporally Restricted by the Circadian Clock Amaury de Montaigu, Markus C. Berns, and Geroge Coupland
10. Identification of Arabidopsis Transcriptional Regulators by Yeast One-Hyrbid Screens Using a Transcription Factor ORFeome Ghislain Breton, Steve A. Kay, and Josй L. Pruneda-Paz
11. Monitoring Alternative Splicing Changes in Arabidopsis Circadian Clock Genes Craig G. Simpson, John Fuller, Cristiane P. G. Calixto, Jim McNicol, Clare Booth, John W. S. Brown, and Dorothee Staiger
12. Assessing the Impact of Photosynthetic Sugars in the Arabidopsis Circadian Clock Michael J. Haydon and Alex A. R. Webb
13. Assessing Protein Stability Under Different Light and Circadian Conditions Takatoshi Kiba and Rossana Henriques
Part IIIAbiotic Stress Responses
14. Screening for Abiotic Stress Tolerance in Rice: Salt, Cold, and Drought Diego M. Almeida, M. Cecнlia Almadanim, Tiago Louren
зo, Isabel A. Abreu, Nelson J. M. Saibo, and M. Margarida Oliveira
15. Basic Techniques to Assess Seed Germination Responses to Abiotic Stress in Arabidopsis Thaliana Urszula Piskurewicz and Luis Lopez-Molina
16. Assessing Tolerance to Heavy-Metal Stress in Arabidopsis Thaliana Seedings Estelle Remy and Paula Duque
17. Assessing Drought Responses Using Thermals Infrared Imaging Ankush Prashar and Hamlyn G. Jones
18. Generating Targeted Gene Knockout Lines in Physcomotrella Patens to Study Evolution of Stress- Responsive Mechanisms Monika Maronova and Maria Kalyna
19. Screening Stress Tolerance Traits in Arabidopsis Cell Cultures Imma P. Salamу, Bogбta Boros, and L
бszlу Szabados
20. Using Arabidopsis Protoplasts to Study Cellular responses to Environmental Stress Ana Confraria and Elena Baena-Gonzбlez
21. Construction of Artificial miRNAs to Prevent Drought Stress in Solanum Tuberosum Anna Wyrzykowska, Marcin Pieczyński, and Zofia Szweykowska-Kuli
Автор: Turksen Kursad Название: Epidermal Cells: Methods and Protocols ISBN: 1071602500 ISBN-13(EAN): 9781071602508 Издательство: Springer Рейтинг: Цена: 18167.00 р. Наличие на складе: Поставка под заказ.
Описание: This fully updated edition brings together a new set of protocols to arm epidermal cell biologists with tools and approaches to continue the quest to uncover the intricacies and regulatory mechanisms underlying epidermal cell development and function.
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