Chemotaxis: Methods and Protocols, Jin Tian, Hereld Dale
Автор: Didenko Vladimir V. Название: In Situ Detection of DNA Damage / Methods and Protocols ISBN: 0896039528 ISBN-13(EAN): 9780896039520 Издательство: Springer Рейтинг: Цена: 5809.00 р. Наличие на складе: Есть (4 шт.) Описание: In In Situ Detection of DNA Damage: Methods and Protocols, Vladimir Didenko and a panel of experts describe all the major in situ techniques for studying DNA damage and apoptosis, and how approaches originally designed to label apoptotic cells can be used for DNA damage analysis (and vice versa). The readily reproducible techniques presented here include all those designed to detect specific single- and double-stranded DNA breaks in tissue sections while using polymerases, ligases, nucleases, and kinases. There are also novel instrumental techniques that use ultrasound for in vivo and in situ detection of apoptotic DNA damage, and in situ applications of flow and laser-scanning cytometry for analysis of DNA strand breaks and apoptosis. State-of-the-art and highly practical, In Situ Detection of DNA Damage: Methods and Protocols presents for the first time a complete set of enzymatic methods for determining DNA breaks, and will greatly facilitate the work of all those studying the in situ detection of DNA damage and the labeling of apoptotic cells.
Автор: Zachariou Michael Название: Affinity Chromatography / Methods and Protocols ISBN: 1588296598 ISBN-13(EAN): 9781588296597 Издательство: Springer Рейтинг: Цена: 2000.00 р. Наличие на складе: Есть (2 шт.) Описание: Thirty-eight years after its introduction, affinity chromatography remains a key tool in th armory of separation techniques available to separation and interaction scientists. Expanded and updated from the first edition, this second edition volume aims to provide the beginner with the practical knowledge needed to develop affinity separations suitable for a variety of applications relevant to the post-genomic era.
Описание: This Methods in Molecular Biology book details approaches for the detection of DNA and RNA including recovery of trace amounts of DNA for amplification and analysis, new qPCR chemistries, step-by-step, reproducible protocols, materials lists, tips and more.
Автор: Piotr Biler Название: Singularities of Solutions to Chemotaxis Systems ISBN: 3110597896 ISBN-13(EAN): 9783110597899 Издательство: Walter de Gruyter Цена: 19330.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: The De Gruyter Series in Mathematics and Life Sciences is devoted to the publication of monographs in the field. They cover topics and methods in fields of current interest that use mathematical approaches to understand and explain, model and influence phenomena in all areas of life sciences. This includes, among others, theory and application of biological mathematical modeling, complex systems biology, bioinformatics, computational biomodeling stochastic modeling, biostatistics, computational evolutionary biology, comparative genomics, or structural bioinformatics. Also, new types of mathematical problems shall be covered that arise from biological knowledge. The main objectives is to make such expositions available to and accessible by an interdisciplinary, growing readership hailing from all disciplines involved. The volumes shall convey the context of the given topic and enable these readers to understand, apply and develop further mathematical methods to given problems in biology. For this reason, works with up to four authors are preferred over edited volumes. Therefore, contributions which are on the borderline of mathematics and life sciences and which stimulate further research at the crossroads of these areas are particularly welcome. In addition, use of electronic media to demonstrate, visualize and model the methods presented are very welcome, especially when interwoven with the written text. Titles in planning include Vlajko L. Kocic and Candace M. Kent, Nonlinear Nonautonomous Difference Equations: Global Behavior and Applications (2019)George Dassios and Athanassios S. Fokas, Electroencephalography and Magnetoencephalography: An Analytical-Numerical Approach (2019)Piotr Biler, Singularities of Solutions to Chemotaxis Systems (2020)
Автор: Jin Название: Chemotaxis ISBN: 1493934783 ISBN-13(EAN): 9781493934782 Издательство: Springer Рейтинг: Цена: 20962.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This second edition expands on the first edition with new chapters describing methods for studyingcell movement, molecular components involved in chemotaxis, spatiotemporaldynamics of signaling components, and quantitative modeling, as well as severalupdated chapters from the first edition. Various methods to investigatedirectional cell growth and movements are presented in Chapters 1-20. Thesechapters contains experimental procedures to visualize and measure migrationbehaviors of different kinds of organisms, including chemotropism in thebudding yeast; cell growth and migration of D. discoideum; border cellmigration in Drosophila; chemotaxis of mouse and human neutrophils; andHIV-induced T cell chemotactic response. Chemotaxis: Methods and Protocols,Second Edition also contains microscopy procedures for studying breast cancercell migration, tumor cell invasion in vivo, and axon guidance. The book concludeswith Chapters 21-27 describing methods that measure spatiotemporal dynamics ofsignaling components involved in chemotaxis; introduce imaging techniques, suchas TRIF, BRET, FRET, and single-molecule microscopy; and mathematical models ofexperimentally generated chemoattractant gradients. Written in the highlysuccessful Methods in Molecular Biology series format, chapters includeintroductions to their respective topics, lists of the necessary materials andreagents, step-by-step, readily reproducible laboratory protocols, and tips ontroubleshooting and avoiding known pitfalls.Cutting edge and thorough,Chemotaxis: Methods and Protocols, Second Edition is a valuable resource foranyone who is interested in the diverse methodologies that are propellingchemotaxis research forward.
Автор: Gбspбri Zoltбn Название: Structural Bioinformatics: Methods and Protocols ISBN: 1071602691 ISBN-13(EAN): 9781071602690 Издательство: Springer Рейтинг: Цена: 16769.00 р. Наличие на складе: Поставка под заказ.
Автор: Wu Jun Название: Thermogenic Fat: Methods and Protocols ISBN: 1493983083 ISBN-13(EAN): 9781493983087 Издательство: Springer Рейтинг: Цена: 19564.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
Preface... Table of Contents... Contributors... Part I Isolation and Culture Methods
1. Isolation, Primary Culture, and Differentiation of Preadipocytes from Mouse Brown Adipose Tissue Wei Gao, Xingxing Kong, and Qin Yang 2. Isolation of Mouse Stromal Vascular Cells for Monolayer Culture Longhua Liu, Louise D. Zheng, Sarah R. Donnelly, Margo P. Emont, Jun Wu, and Zhiyong Cheng 3. Flow Cytometry Assisted Isolation of Adipose Tissue Derived Stem Cells Umesh D. Wankhade and Sushil G. Rane 4. Flow Cytometric Isolation and Differentiation of Adipogenic Progenitor Cells into Brown and Brite/Beige Adipocytes Jochen Steinbring, Antonia Graja, Anne-Marie Jank, and Tim J. Schulz 5. Immuno-Magnetic Isolation and Thermogenic Differentiation of White Adipose Tissue Progenitor Cells Rohollah Babaeikelishomi, Irem Bayindir-Buchhalter, Irina Meln, and Alexandros Vegiopoulos 6. Isolation of Immune Cells from Adipose TissueJonathan R. Brestoff 7. Differentiation and Metabolic Interrogation of Human Adipocytes Nicki A. Baker, Lindsey A. Muir, Carey N. Lumeng, and Robert W. O'Rourke 8. Protocols for Generation of Immortalized Human Brown and White Preadipocyte Cell Lines Farnaz Shamsi and Yu-Hua Tseng Part II Functional Analysis, Bioengineering, and Other Applications 9. Gene Expression and Histological Analysis of Activated Brown Adipocytes in Adipose Tissue Yun-Hee Lee 10. Genetic Mouse Models: The Powerful Tools to Study Fat Tissues Xingxing Kong, Kevin W. Williams, and Tiemin Liu 11. Genetic Manipulation with Viral Vectors to Assess Metabolism and Adipose Tissue Function Nicolбs Gуmez-Banoy and James C. Lo 12. Bioenergetic Analyses in Adipose Tissue Lawrence Kazak 13. Oxygen Consumption Rate and Energy Expenditure in Mice: Indirect Calorimetry Eun Ran Kim and Qingchun Tong 14. Isolation and Patch-Clamp of Primary Adipocytes Yanhui Zhang, Dan Tong, Anil Mishra, Litao Xie, and Rajan Sah 15. In Vitro Approaches to Model and Study Communications Between Adipose Tissue and the Liver Sean O'Connor and Paul Cohen 16. Identification and Quantification of Human Brown Adipose Tissue Maria Chondronikola, Craig Porter, John O. Ogunbileje, and Labros S. Sidossis 17. Designing 3D Adipospheres for Quantitative Metabolic Study Takeshi Akama, Brendan M. Leung, Joe Labuz, Shuichi Takayama, and Tae-Hwa Chun 18. Culture and Sampling of Primary Adipose Tissue in Practical Microfluidic Systems Jessica C. Brooks, Robert L. Judd, and Christopher J. Easley 19. Using Thermogenic Beige Cells to Identify Biologically Active Small Molecules and Peptides Ling Wu and Bin Xu
Автор: Yokomori Kyoko, Shirahige Katsuhiko Название: Cohesin and Condensin: Methods and Protocols ISBN: 149398232X ISBN-13(EAN): 9781493982325 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: The volume provides comprehensive, state-of-the-art experimental techniques that are now available to dissect the molecular mechanisms of regulation and function of cohesin and the related factor condensin in vitro and in vivo across different model organisms, as well as in human cells.
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