Stem Cell Heterogeneity: Methods and Protocols, Turksen Kursad
Автор: Didenko Vladimir V. Название: In Situ Detection of DNA Damage / Methods and Protocols ISBN: 0896039528 ISBN-13(EAN): 9780896039520 Издательство: Springer Рейтинг: Цена: 5809.00 р. Наличие на складе: Есть (4 шт.) Описание: In In Situ Detection of DNA Damage: Methods and Protocols, Vladimir Didenko and a panel of experts describe all the major in situ techniques for studying DNA damage and apoptosis, and how approaches originally designed to label apoptotic cells can be used for DNA damage analysis (and vice versa). The readily reproducible techniques presented here include all those designed to detect specific single- and double-stranded DNA breaks in tissue sections while using polymerases, ligases, nucleases, and kinases. There are also novel instrumental techniques that use ultrasound for in vivo and in situ detection of apoptotic DNA damage, and in situ applications of flow and laser-scanning cytometry for analysis of DNA strand breaks and apoptosis. State-of-the-art and highly practical, In Situ Detection of DNA Damage: Methods and Protocols presents for the first time a complete set of enzymatic methods for determining DNA breaks, and will greatly facilitate the work of all those studying the in situ detection of DNA damage and the labeling of apoptotic cells.
Автор: Zachariou Michael Название: Affinity Chromatography / Methods and Protocols ISBN: 1588296598 ISBN-13(EAN): 9781588296597 Издательство: Springer Рейтинг: Цена: 2000.00 р. Наличие на складе: Есть (2 шт.) Описание: Thirty-eight years after its introduction, affinity chromatography remains a key tool in th armory of separation techniques available to separation and interaction scientists. Expanded and updated from the first edition, this second edition volume aims to provide the beginner with the practical knowledge needed to develop affinity separations suitable for a variety of applications relevant to the post-genomic era.
Автор: Turksen Название: Human Embryonic Stem Cell Protocols ISBN: 1607613689 ISBN-13(EAN): 9781607613688 Издательство: Springer Рейтинг: Цена: 14673.00 р. 20962.00-30% Наличие на складе: Есть (1 шт.) Описание: Controversy surrounds the study of human embryonic stem cells. In this book, respected researchers expand upon the popular first edition and describe in detail their most useful techniques for the molecular and cellular manipulation of these intriguing cells.
Описание: Research has revealed that natural killer (NK) cells are sentinels of the innate immune response. This text offers detailed protocols plus the latest methods and techniques designed to study NK cells within specialized tissue sites in both mice and humans.
Автор: Perdiguero Eusebio, Cornelison Ddw Название: Muscle Stem Cells: Methods and Protocols ISBN: 1493982966 ISBN-13(EAN): 9781493982967 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume provides leading-edge protocols in the study of the molecular and cellular biology of muscle stem cells.
Автор: Birbrair Alexander Название: Stem Cells Heterogeneity in Different Organs ISBN: 3030241106 ISBN-13(EAN): 9783030241100 Издательство: Springer Рейтинг: Цена: 18167.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This book presents a comprehensive discussion on the heterogeneity existing between different types of stem cells within the same tissue. As the functions of these stem cells vary, this is very important for the application of stem cells in cell therapy. This book describes the many recent developments that have revealed completely different roles of distinct stem cells within the same organ. Stem Cells Heterogeneity in Different Organs provides a timely update on the current information on stem cells heterogeneity in various tissues. It also provides a solid foundation of the history of stem cells from specific tissues and the current applications of this knowledge in regenerative medicine. Taken with its companion volumes, Stem Cells Heterogeneity: Novel Concepts and Stem Cells Heterogeneity in Cancer, this book is essential reading for advanced cell biology students as well as researchers and clinicians working with stem cells.
Автор: Birbrair Alexander Название: Stem Cells Heterogeneity in Cancer ISBN: 3030143686 ISBN-13(EAN): 9783030143688 Издательство: Springer Рейтинг: Цена: 16070.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This book presents a comprehensive discussion on the heterogeneity existing between different types of stem cells within the same tissue, for several types of cancers, e.g. glioblastoma stem cells. Recent developments have revealed completely different roles of distinct stem cells within the same organ. Thus, Stem Cells Heterogeneity in Cancer provides a timely update us on the current information on stem cells heterogeneity in various tissues. It also provides a solid foundation of the history of stem cells from specific tissues and the current applications of this knowledge in regenerative medicine. When taken as a whole, alongside its companion volumes Stem Cells Heterogeneity – Novel Concepts, and Stem Cells Heterogeneity in Different Organs, these three books present a comprehensive reference on stem cell heterogeneity in various tissues and current and future applications for regenerative medicine. It is essential reading for advanced cell biology students as well as researchers in stem cells and clinicians.
Автор: Alexander Birbrair Название: Stem Cells Heterogeneity - Novel Concepts ISBN: 3030110958 ISBN-13(EAN): 9783030110956 Издательство: Springer Рейтинг: Цена: 20962.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This book presents a comprehensive discussion on the novel concepts in stem cell heterogeneity, from pluripotent stem cells to human mesenchymal stem cells, adult and cancer stem cells of the thyroid, sarcoma, and more. Thus, Stem Cell Heterogeneity - Novel Concepts starts from a timely update on the current information on stem cells heterogeneity in various tissues and discusses new concepts and future directions. It also provides a solid foundation of the history of stem cells from specific tissues and the current applications of this knowledge in regenerative medicine. When taken as a whole, alongside its companion volumes Stem Cells Heterogeneity in Different Organs, and Stem Cells Heterogeneity in Cancer, these three books present a comprehensive reference on stem cell heterogeneity in various tissues and current and future applications for regenerative medicine. It is essential reading for advanced cell biology students as well as researchers in stem cells and clinicians.
Автор: Natasha S. Barteneva; Ivan A. Vorobjev Название: Cellular Heterogeneity ISBN: 1493985418 ISBN-13(EAN): 9781493985418 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Поставка под заказ.
Описание: This volume is essential for anyone struggling with the cellular heterogeneity field, and it connects this new area with the latest advances in innovative techniques combined with practical advice from international experts. The chapters provide a comprehensive overview of cellular heterogeneity analysis techniques, exploring cytometry, imaging and spectroscopy methods, metabolic and molecular biological methods in tracking, as well as chromatin and cell cycle heterogeneity. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, detailed lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and accessible, Cellular Heterogeneity: Methods and Protocols encourages readers to explore new ways of studying cells that will help lead to exciting new discoveries.
Автор: Koledova Zuzana Название: 3D Cell Culture: Methods and Protocols ISBN: 1493983709 ISBN-13(EAN): 9781493983704 Издательство: Springer Рейтинг: Цена: 17468.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: 1. 3D Cell Culture: An Introduction
Zuzana Koledova
Part I: Hydrogels and Scaffolds for 3D Cell Culture
2. Preparation of Decellularized Biological Scaffolds for 3D Cell Culture
Bryan N. Brown, Michael J. Buckenmeyer, and Travis A. Prest
3. 3D Cell Culture in Interpenetrating Networks of Alginate and rBM Matrix
Katrina Wisdom and Ovijit Chaudhuri
4. Hydrogel-Based In Vitro Models of Tumor Angiogenesis
Laura J. Bray, Marcus Binner, Uwe Freudenberg, and Carsten Werner
5. Generation of Induced Pluripotent Stem Cells in Defined Three-Dimensional PEG Hydrogels
Massimiliano Caiazzo, Yoji Tabata, and Matthias Lutolf
6. Calcium Phosphate Foams: Potential Scaffolds for Bone Tissue Modelling in Three-Dimensions
Edgar B. Montufar, Lucy Vojtova, Ladislav Celko, and Maria-Pau Ginebra
Part II: 3D Organoid and Organotypic Cultures
7. Establishment of 3D Intestinal Organoid Cultures from Intestinal Stem Cells
Shinya Sugimoto and Toshiro Sato
8. 3D Co-Culture of Mammary Organoids with Fibrospheres: A Model for Studying Epithelial-Stromal Interactions During Mammary Branching Morphogenesis
Zuzana Koledova
9. An Organotypic 3D Assay for Primary Human Mammary Epithelial Cells that Recapitulates Branching Morphogenesis
Jelena R. Linnemann, Lisa K. Meixner, Haruko Miura, and Christina H. Scheel
10. 3D Primary Culture Model to Study Human Mammary Development
Daniel H. Miller, Ethan S. Sokol, and Piyush B. Gupta
11. Lungosphere Assay: 3D Culture of Lung Epithelial Stem/Progenitor Cells
Anas Rabata, Ales Hampl, and Zuzana Koledova
12. 3D Hanging Drop Culture to Establish Prostate Cancer Organoids
Theresa Eder and Iris E. Eder
13. 3D Fluid-Dynamic Culture Models of Multiple Myeloma
Marina Ferrarini, Nathalie Steimberg, Jennifer Boniotti, Angiola Berenzi, Daniela Belloni, Giovanna Mazzoleni, and Elisabetta Ferrero
14. Preparation of a Three-Dimensional Full Thickness Skin Equivalent
Christian Reuter, Heike Walles, and Florian Groeber
15. Analysis of Breast Cancer Cell Invasion Using an Organotypic Culture System
Romana E. Ranftl and Fernando Calvo
16. 3D Co-Culture Model of the Brain Parenchyma/Metastasis Interface of Brain Metastasis
5. High-Efficiency Transfection of Glioblastoma Cells and a Simple Spheroid Migration Assay
Carsten Hagemann, Diana Amend, Almuth F. Kessler, Thomas Linsenmann, Ralf-Ingo Ernestus, and Mario Lцhr
6. Construction and Application of Random dsRNA Interference Library for Functional Genetic Screens in Embryonic Stem Cells
Xiaoxing Cheng and Rui Jian
7. Efficient Depletion of Essential Gene Products for Loss-of-Function Studies in Embryonic Stem Cells
Soizik Berlivet, Isabelle Hmitou, Hйlиne Picaud, and Matthieu Gйrard
8. Regulation and/or Repression of Cholinergic Differentiation of Murine Embryonic Stem Cells Using RNAi Directed Against Transcription Factor L3/Lhx8
Takayuki Manabe and Akio Wanaka
9. A Recessive Genetic Screen for Components of the RNA Interference Pathway Performed in Mouse Embryonic Stem Cells
Melanie I. Trombly and Xiaozhong Wang
10. RNAi Knockdown of Ape1 Gene in the Differentiation of Mouse Embryonic Stem Cells
Gang-Ming Zou, Jieqing Yu, Cynthia LeBron, and Yumei Fu
11. An Efficient Transfection Method for Differentiation and Cell Proliferation of Mouse Embryonic Stem Cells
Jun-Yang Liou, Bor-Sheng Ko, and Tzu-Ching Chang
12. Implanting Glioblastoma Spheroids into Rat Brains and Monitoring Tumor Growth by MRI Volumetry
Mario Lцhr, Thomas Linsenmann, Anna Jawork, Almuth F. Kessler, Nils Timmermann, Gyцrgy A. Homola, Ralf-Ingo Ernestus, and Carsten Hagemann
13. RNAi-Based Techniques for the Analysis of Gene Function in Drosophila Germline Stem Cells
Amelia J. Helms, Danielle S. Finger, Victoria L. Hardy, and Elizabeth T. Ables
14. In Vivo RNAi in the Drosophila Follicular Epithelium: Analysis of Stem Cell Maintenance, Proliferation, and Differentiation
Veit Riechmann
15. A Phenotype-Based RNAi Screening for Ras-ERK/MAPK Signaling-Associated Stem Cell Regulators in C. elegans
Myon-Hee Lee and Dong Suk Yoon
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Автор: Ivan A. Vorobjev Название: Cellular Heterogeneity Methods and Protocols ISBN: 1493976796 ISBN-13(EAN): 9781493976799 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume is essential for anyone struggling with the cellular heterogeneity field, and it connects this new area with the latest advances in innovative techniques combined with practical advice from international experts.
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