Stem Cell Heterogeneity: Methods and Protocols, Turksen Kursad
Автор: Didenko Vladimir V. Название: In Situ Detection of DNA Damage / Methods and Protocols ISBN: 0896039528 ISBN-13(EAN): 9780896039520 Издательство: Springer Рейтинг: Цена: 5809.00 р. Наличие на складе: Есть (4 шт.) Описание: In In Situ Detection of DNA Damage: Methods and Protocols, Vladimir Didenko and a panel of experts describe all the major in situ techniques for studying DNA damage and apoptosis, and how approaches originally designed to label apoptotic cells can be used for DNA damage analysis (and vice versa). The readily reproducible techniques presented here include all those designed to detect specific single- and double-stranded DNA breaks in tissue sections while using polymerases, ligases, nucleases, and kinases. There are also novel instrumental techniques that use ultrasound for in vivo and in situ detection of apoptotic DNA damage, and in situ applications of flow and laser-scanning cytometry for analysis of DNA strand breaks and apoptosis. State-of-the-art and highly practical, In Situ Detection of DNA Damage: Methods and Protocols presents for the first time a complete set of enzymatic methods for determining DNA breaks, and will greatly facilitate the work of all those studying the in situ detection of DNA damage and the labeling of apoptotic cells.
Автор: Zachariou Michael Название: Affinity Chromatography / Methods and Protocols ISBN: 1588296598 ISBN-13(EAN): 9781588296597 Издательство: Springer Рейтинг: Цена: 2000.00 р. Наличие на складе: Есть (2 шт.) Описание: Thirty-eight years after its introduction, affinity chromatography remains a key tool in th armory of separation techniques available to separation and interaction scientists. Expanded and updated from the first edition, this second edition volume aims to provide the beginner with the practical knowledge needed to develop affinity separations suitable for a variety of applications relevant to the post-genomic era.
Автор: Turksen Название: Human Embryonic Stem Cell Protocols ISBN: 1607613689 ISBN-13(EAN): 9781607613688 Издательство: Springer Рейтинг: Цена: 14673.00 р. 20962.00-30% Наличие на складе: Есть (1 шт.) Описание: Controversy surrounds the study of human embryonic stem cells. In this book, respected researchers expand upon the popular first edition and describe in detail their most useful techniques for the molecular and cellular manipulation of these intriguing cells.
Автор: Koledova Zuzana Название: 3D Cell Culture: Methods and Protocols ISBN: 1493983709 ISBN-13(EAN): 9781493983704 Издательство: Springer Рейтинг: Цена: 17468.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: 1. 3D Cell Culture: An Introduction
Zuzana Koledova
Part I: Hydrogels and Scaffolds for 3D Cell Culture
2. Preparation of Decellularized Biological Scaffolds for 3D Cell Culture
Bryan N. Brown, Michael J. Buckenmeyer, and Travis A. Prest
3. 3D Cell Culture in Interpenetrating Networks of Alginate and rBM Matrix
Katrina Wisdom and Ovijit Chaudhuri
4. Hydrogel-Based In Vitro Models of Tumor Angiogenesis
Laura J. Bray, Marcus Binner, Uwe Freudenberg, and Carsten Werner
5. Generation of Induced Pluripotent Stem Cells in Defined Three-Dimensional PEG Hydrogels
Massimiliano Caiazzo, Yoji Tabata, and Matthias Lutolf
6. Calcium Phosphate Foams: Potential Scaffolds for Bone Tissue Modelling in Three-Dimensions
Edgar B. Montufar, Lucy Vojtova, Ladislav Celko, and Maria-Pau Ginebra
Part II: 3D Organoid and Organotypic Cultures
7. Establishment of 3D Intestinal Organoid Cultures from Intestinal Stem Cells
Shinya Sugimoto and Toshiro Sato
8. 3D Co-Culture of Mammary Organoids with Fibrospheres: A Model for Studying Epithelial-Stromal Interactions During Mammary Branching Morphogenesis
Zuzana Koledova
9. An Organotypic 3D Assay for Primary Human Mammary Epithelial Cells that Recapitulates Branching Morphogenesis
Jelena R. Linnemann, Lisa K. Meixner, Haruko Miura, and Christina H. Scheel
10. 3D Primary Culture Model to Study Human Mammary Development
Daniel H. Miller, Ethan S. Sokol, and Piyush B. Gupta
11. Lungosphere Assay: 3D Culture of Lung Epithelial Stem/Progenitor Cells
Anas Rabata, Ales Hampl, and Zuzana Koledova
12. 3D Hanging Drop Culture to Establish Prostate Cancer Organoids
Theresa Eder and Iris E. Eder
13. 3D Fluid-Dynamic Culture Models of Multiple Myeloma
Marina Ferrarini, Nathalie Steimberg, Jennifer Boniotti, Angiola Berenzi, Daniela Belloni, Giovanna Mazzoleni, and Elisabetta Ferrero
14. Preparation of a Three-Dimensional Full Thickness Skin Equivalent
Christian Reuter, Heike Walles, and Florian Groeber
15. Analysis of Breast Cancer Cell Invasion Using an Organotypic Culture System
Romana E. Ranftl and Fernando Calvo
16. 3D Co-Culture Model of the Brain Parenchyma/Metastasis Interface of Brain Metastasis
Raquel Blazquez and Tobias Pukrop
Part III: Micropatterning
Автор: Di Nardo Paolo, Dhingra Sanjiv, Singla Dinender K. Название: Adult Stem Cells: Methods and Protocols ISBN: 1493982931 ISBN-13(EAN): 9781493982936 Издательство: Springer Рейтинг: Цена: 16769.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
Preface... Table of Contents... Contributing Authors...
1. A Simple Protocol to Isolate, Characterize and Expand Dental Pulp Stem Cells Federica Di Scipio, Andrea Elio Sprio, Maria Elisabetta Carere, Zhiqian Yang, and Giovanni Nicolao Berta 2. Derivation of Mesenchymal Stem cells from Embryonic Stem Cells: A Non-Variable and Inexhaustive Source of Adult Stem Cells Glen Lester Sequiera, Niketa Sareen, Ejlal Abu Al-Rub, and Sanjiv Dhingra 3. Compact Bone-Derived Multipotent Mesenchymal Stromal Cells (MSCs) for the Treatment of Sjogren's-Like Disease in NOD Mice Ghada Abu Elghanam, Younan Liu, Saeed Khalili, Dongdong Fang, and Simon D.Tran 4. Manipulating the Proliferative Potential of Cardiomyocytes by Gene Transfer Giulia Prosdocimo and Mauro Giacca 5. Assessment of Energy Metabolic Changes in Adipose Tissue-Derived Stem Cells Ghazaleh Hajmousa and Martin C. Harmsen 6. Isolation and In Vitro Characterization of Epidermal Stem Cells Kasper S. Moestrup, Marianne S. Andersen, and Kim B. Jensen 7. Endothelial Progenitor Cells: Procedure for Cell Isolation and Characterization Garikipati V.N. Srikanth, Zhongjian Cheng, and Raj Kishore 8. Therapeutic Application of Placental Mesenchymal Stem Cells Reprogrammed Neurospheres in Spinal Cord Injury of SCID Vikram Sabapathy, Franklin Jebaraj Herbert, and Sanjay Kumar 9. Therapeutic Application of Human Wharton Jelly Mesenchymal Stem Cells in Skin Injury of SCID Vikram Sabapathy, Balasubramanian Sundaram, and Sanjay Kumar 10. Isolation, Characterization, and Expansion of Cancer Stem Cells Luke A. Torre-Healy, Artem Berezovsky, and Justin D. Lathia 11. Cardiac Progenitor Cell Isolation from Human Auricles Paolo Di Nardo and Francesca Pagliari 12. Isolation and Culture of Satellite Cells from Mouse Skeletal Muscle Antonio Musarт and Silvia Carosio 13. Isolation of Stromal Stem Cells from Adipose Tissue Maria Prat, Francesca Oltolina, Silvia Antonini, and Andrea Zamperone 14. Human Induced Pluripotent Stem Cell-Derived Mesencymal Stem Cells as an Individual-Specific and Renewable Source of Adult Stem Cells15. A Simplified and Systematic Method to Isolate, Culture and Characterize Multiple Types of Human Dental Stem Cells from a Single Tooth Mohammed Bakkar, Younan Liu, Dongdong Fang, Camille Stegen, Xinyun Su, Murali Ramamoorthi, Li-Chieh Lin, Takako Kawasaki, Nicholas Makhoul, Huan Pham, Yoshinori Sumita, and Simon D. Tran 16. Measurement of Autophagy by Flow Cytometry Silvia Zappavigna, Angela Lombardi, Gabriella Misso, Anna Grimaldi, and Michele Caraglia 17. Echocardiography-Guided Intramyocardial Injection Method in a Murine Model Kay Maeda, Rick Seymour, Marc Ruel, and Erik J. Suuronen 18. Noninvasive Assessment of Cell Fate and Biology in Transplanted Mesenchymal Stem Cells Federico Franchi and Martin Rodriguez-Porcel 19. Methods for Long Term Storage of Murine Bone Marrow Derived Mesenchymal Stem Cells Niketa Sareen, Ejlal Abu-El-Rub, Glen Lester Sequiera, Meenal Moudgil, and Sanjiv Dhingra 20. Therapeutic Application of Adult Stem Cells in the Heart Taylor A. Johnson and Dinender K. Singla 21. Patient-Derived and Intra-Operatively Formed Biomaterial for Tissue Engineering Shalmli U. Joshi, Rares O. Barbu, Melissa Carr-Reynolds, Brian Barnes, and Syam P. Nukavarapu 22. Three-Dimensional Printed Scaffolds with Multipotent Mesenchymal Stromal Cells for Rabbit Mandibular Reconstruction and Engineering Dongdong Fang, Michael Roskies, Mohamed-Nur Abdallah, Mohammed Bakkar, Jack Jordan, Li-Chieh Lin, Faleh Tamimi, and Simon D. Tran 23. Optimal Enviro
Описание: Research has revealed that natural killer (NK) cells are sentinels of the innate immune response. This text offers detailed protocols plus the latest methods and techniques designed to study NK cells within specialized tissue sites in both mice and humans.
Автор: Perdiguero Eusebio, Cornelison Ddw Название: Muscle Stem Cells: Methods and Protocols ISBN: 1493982966 ISBN-13(EAN): 9781493982967 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume provides leading-edge protocols in the study of the molecular and cellular biology of muscle stem cells.
Автор: Ivan A. Vorobjev Название: Cellular Heterogeneity Methods and Protocols ISBN: 1493976796 ISBN-13(EAN): 9781493976799 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume is essential for anyone struggling with the cellular heterogeneity field, and it connects this new area with the latest advances in innovative techniques combined with practical advice from international experts.
Автор: Turksen Название: Stem Cell Heterogeneity ISBN: 1493965492 ISBN-13(EAN): 9781493965496 Издательство: Springer Рейтинг: Цена: 19564.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Given the variety of studies and data that have suggested the existence of heterogeneous populations or subpopulations of stem cells, this detailed volume examines different aspects of stem cell heterogeneity. This goes against the long-held tenet that stem cells, defined by their capacity for self-renewal and lineage development, comprised a homogenous population, thus providing the reader with a new avenue of exploration into the complex world of stem cell study. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Stem Cell Heterogeneity: Methods and Protocols serves as an ideal guide for investigators exploring this important area of research.
Автор: Birbrair Alexander Название: Stem Cells Heterogeneity in Different Organs ISBN: 3030241106 ISBN-13(EAN): 9783030241100 Издательство: Springer Рейтинг: Цена: 18167.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This book presents a comprehensive discussion on the heterogeneity existing between different types of stem cells within the same tissue. As the functions of these stem cells vary, this is very important for the application of stem cells in cell therapy. This book describes the many recent developments that have revealed completely different roles of distinct stem cells within the same organ. Stem Cells Heterogeneity in Different Organs provides a timely update on the current information on stem cells heterogeneity in various tissues. It also provides a solid foundation of the history of stem cells from specific tissues and the current applications of this knowledge in regenerative medicine. Taken with its companion volumes, Stem Cells Heterogeneity: Novel Concepts and Stem Cells Heterogeneity in Cancer, this book is essential reading for advanced cell biology students as well as researchers and clinicians working with stem cells.
Автор: Alexander Birbrair Название: Stem Cells Heterogeneity in Different Organs ISBN: 3030241076 ISBN-13(EAN): 9783030241070 Издательство: Springer Рейтинг: Цена: 25155.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
This book presents a comprehensive discussion on the heterogeneity existing between different types of stem cells within the same tissue. As the functions of these stem cells vary, this is very important for the application of stem cells in cell therapy. This book describes the many recent developments that have revealed completely different roles of distinct stem cells within the same organ.
Stem Cells Heterogeneity in Different Organs provides a timely update on the current information on stem cells heterogeneity in various tissues. It also provides a solid foundation of the history of stem cells from specific tissues and the current applications of this knowledge in regenerative medicine. Taken with its companion volumes, Stem Cells Heterogeneity: Novel Concepts and Stem Cells Heterogeneity in Cancer, this book is essential reading for advanced cell biology students as well as researchers and clinicians working with stem cells.
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