Protein Downstream Processing: Design, Development, and Application of High and Low-Resolution Methods, Labrou Nikolaos E.

Автор: Nikolaos E. Labrou
Название: Protein Downstream Processing
ISBN: 1493960407 ISBN-13(EAN): 9781493960408
Издательство: Springer
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Цена: 19564.00 р.
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Описание:

Part I. Screening and Design Purification Strategies

1. Protein Purification: An Overview

Nikolaos Labrou

2. Establishment of a Design Space for Biopharmaceutical Purification Processes Using DoE

Ignacio Amadeo, Laura Mauro, Eduardo Ortн, and Guillermina Forno

3. High Throughput Process Development: I. Process Chromatography

A. S. Rathore and R. Bhambure

4. High Throughput Process Development: II. Membrane Chromatography

A. S. Rathore and S. Muthukumar

5. Media Selection in Ion Exchange Chromatography in a Single Microplate

Charlotte Cabanne and Xavier Santarelli

6. High Throughput Screening of Dye-Ligands for Chromatography

Sunil Kumar and Narayan S. Punekar

7. Measurement of Uptake Curves and Adsorption Isotherms by Automated Microscale Chromatography Pipette Tips

Sunil Chhatre and Daniel G. Bracewell

Part II. Low-Resolution Protein Purification Methods

8. Recovery of Recombinant Proteins from Plants Using Aqueous Two-Phase Partitioning Systems: An Outline

Zhengrong GU

9. Aqueous Two-Phase Systems Strategies for the Recovery of Proteins from Plants

Oscar Aguilar and Marco Rito-Palomares

10. Aqueous Two Phase Assisted Precipitation of Proteins: A Platform for Isolation of Process Related Impurities from Therapeutic Proteins

A. S. Rathore and R. Bhambure

11. Simultaneous Purification and Refolding of Proteins by Affinity Precipitation and Macro-(Affinity Ligand) Facilitated Three Phase Partitioning (MLFTPP)

Ipsita Roy, Kalyani Mondal, and Munishwar N. Gupta

12. Co-Expression and Co-Purification of Antigen-Antibody Complexes in Bacterial Cytoplasm and Periplasm

Ario de Marco

13. Immunoglobulin Purification by Caprylic Acid

Victor Morais, Patricia Berasain, and Hugo Massaldi

Part III: High-Resolution Protein Purification Methods

14. Affinity Tags in Protein Purification and Peptide Enrichment: An Overview

Ana Sofia Pina, Нris L. Batalha, and Ana Cecнlia A. Roque

15. Expression and Purification of Large Active GST Fusion Enzymes

Stefania Deceglie, Claudia Lionetti, Marina Roberti, Palmiro Cantatore, and Paola Loguercio Polosa

16. Synthetic Ligand Affinity Chromatography. Purification of Human Serum Albumin and Related Fusion Proteins

Sharon Williams, Phil Morton, and Dev Baines

17. Zbasic - A Purification Tag for Selective Ion-Exchange Recovery

My Hedhammar, Johan Nilvebrant, and Sophia Hober

18. An Orthogonal Fusion Tag for Efficient Protein Purification

Johan Nilvebrant, Mikael Еstrand, and Sophia Hober

19. Phage Display of Engineered Binding Proteins

Mark Levisson, Ruud B. Spruijt, Ingrid Nolla Winkel, Servй W.M. Kengen, and John van der Oost

20. Biomimetic Affinity Ligands for Protein Purification

Isabel T. Sousa and M. Вngela Taipa

21. Synthesis and Application of Dye-Ligand Affinity Adsorbents

Evangelia Chronopoulou and Nikolaos E. Labrou

22. Peptide Affinity Chromatography Based on Combinatorial Strategies for Protein Purification

S. A. Camperi, M. C. Martнnez-Ceron, S. L. Giudicessi, M. M. Marani, F. Albericio, and O. Cascone

23. Affinity Chromatography of Proteins on Monolithic Columns

E.G. Vlakh, G.A. Platonova, and T.B. Tennikova

24. Sample Displacement Batch Chromatography of Proteins

Marta Kotasinska, Verena Richter, Marcel Kwiatkowski, and Hartmut Schlьter

Part IV. Assessing Protein Structural Integrity, Purity, and Stabilization

25. Analysis of Host-Cell Proteins in Biotherapeutic Proteins by LC/MS Approaches

Catalin E. Doneanu, Weibin Chen, an

Автор: Mathias Munschauer
Название: High-Resolution Profiling of Protein-RNA Interactions
ISBN: 3319162527 ISBN-13(EAN): 9783319162522
Издательство: Springer
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Цена: 18167.00 р.
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Описание: The work reported in this book represents an excellent example of how creative experimentation and technology development, complemented by computational data analysis, can yield important insights that further our understanding of biological entities from a systems perspective. The book describes how the study of a single RNA-binding protein and its interaction sites led to the development of the novel ‘protein occupancy profiling’ technology that for the first time captured the mRNA sequence space contacted by the ensemble of expressed RNA binders. Application of protein occupancy profiling to eukaryotic cells revealed that extensive sequence stretches in 3’ UTRs can be contacted by RBPs and that evolutionary conservation as well as negative selection act on protein-RNA contact sites, suggesting functional importance. Comparative analysis of the RBP-bound sequence space has the potential to unravel putative cis-acting RNA elements without a priori knowledge of the bound regulators. Here, Dr. Munschauer provides a comprehensive introduction to the field of post-transcriptional gene regulation, examines state-of-the-art technologies, and combines the conclusions from several journal articles into a coherent and logical story from the frontiers of systems-biology inspired life science. This thesis, submitted to the Department of Biology, Chemistry and Pharmacy at Freie Universit?t Berlin, was selected as outstanding work by the Berlin Institute for Medical Systems Biology at the Max-Delbrueck Center for Molecular Medicine, Germany.

Автор: Nikolaos E. Labrou
Название: Protein Downstream Processing
ISBN: 1627039767 ISBN-13(EAN): 9781627039765
Издательство: Springer
Рейтинг:
Цена: 30745.00 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание:

Part I. Screening and Design Purification Strategies

1. Protein Purification: An Overview

Nikolaos Labrou

2. Establishment of a Design Space for Biopharmaceutical Purification Processes Using DoE

Ignacio Amadeo, Laura Mauro, Eduardo Ortн, and Guillermina Forno

3. High Throughput Process Development: I. Process Chromatography

A. S. Rathore and R. Bhambure

4. High Throughput Process Development: II. Membrane Chromatography

A. S. Rathore and S. Muthukumar

5. Media Selection in Ion Exchange Chromatography in a Single Microplate

Charlotte Cabanne and Xavier Santarelli

6. High Throughput Screening of Dye-Ligands for Chromatography

Sunil Kumar and Narayan S. Punekar

7. Measurement of Uptake Curves and Adsorption Isotherms by Automated Microscale Chromatography Pipette Tips

Sunil Chhatre and Daniel G. Bracewell

Part II. Low-Resolution Protein Purification Methods

8. Recovery of Recombinant Proteins from Plants Using Aqueous Two-Phase Partitioning Systems: An Outline

Zhengrong GU

9. Aqueous Two-Phase Systems Strategies for the Recovery of Proteins from Plants

Oscar Aguilar and Marco Rito-Palomares

10. Aqueous Two Phase Assisted Precipitation of Proteins: A Platform for Isolation of Process Related Impurities from Therapeutic Proteins

A. S. Rathore and R. Bhambure

11. Simultaneous Purification and Refolding of Proteins by Affinity Precipitation and Macro-(Affinity Ligand) Facilitated Three Phase Partitioning (MLFTPP)

Ipsita Roy, Kalyani Mondal, and Munishwar N. Gupta

12. Co-Expression and Co-Purification of Antigen-Antibody Complexes in Bacterial Cytoplasm and Periplasm

Ario de Marco

13. Immunoglobulin Purification by Caprylic Acid

Victor Morais, Patricia Berasain, and Hugo Massaldi

Part III: High-Resolution Protein Purification Methods

14. Affinity Tags in Protein Purification and Peptide Enrichment: An Overview

Ana Sofia Pina, Нris L. Batalha, and Ana Cecнlia A. Roque

15. Expression and Purification of Large Active GST Fusion Enzymes

Stefania Deceglie, Claudia Lionetti, Marina Roberti, Palmiro Cantatore, and Paola Loguercio Polosa

16. Synthetic Ligand Affinity Chromatography. Purification of Human Serum Albumin and Related Fusion Proteins

Sharon Williams, Phil Morton, and Dev Baines

17. Zbasic - A Purification Tag for Selective Ion-Exchange Recovery

My Hedhammar, Johan Nilvebrant, and Sophia Hober

18. An Orthogonal Fusion Tag for Efficient Protein Purification

Johan Nilvebrant, Mikael Еstrand, and Sophia Hober

19. Phage Display of Engineered Binding Proteins

Mark Levisson, Ruud B. Spruijt, Ingrid Nolla Winkel, Servй W.M. Kengen, and John van der Oost

20. Biomimetic Affinity Ligands for Protein Purification

Isabel T. Sousa and M. Вngela Taipa

21. Synthesis and Application of Dye-Ligand Affinity Adsorbents

Evangelia Chronopoulou and Nikolaos E. Labrou

22. Peptide Affinity Chromatography Based on Combinatorial Strategies for Protein Purification

S. A. Camperi, M. C. Martнnez-Ceron, S. L. Giudicessi, M. M. Marani, F. Albericio, and O. Cascone

23. Affinity Chromatography of Proteins on Monolithic Columns

E.G. Vlakh, G.A. Platonova, and T.B. Tennikova

24. Sample Displacement Batch Chromatography of Proteins

Marta Kotasinska, Verena Richter, Marcel Kwiatkowski, and Hartmut Schlьter

Part IV. Assessing Protein Structural Integrity, Purity, and Stabilization

25. Analysis of Host-Cell Proteins in Biotherapeutic Proteins by LC/MS Approaches

Catalin E. Doneanu, Weibin Chen, an