Автор: Rustgi Sachin, Luo Hong Название: Biolistic DNA Delivery in Plants: Methods and Protocols ISBN: 1071603558 ISBN-13(EAN): 9781071603550 Издательство: Springer Рейтинг: Цена: 20962.00 р. Наличие на складе: Поставка под заказ.
Описание: This volume details protocols for the use of the biolistic DNA delivery method in different plant species. Chapters guide readers through non-protocol chapters that cover relevant topics of interest, a broad overview of the field, exciting modifications of the system, and reliable plant transformation procedures in different plant species. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Biolistic DNA Delivery: Methods and Protocols aims to provide a comprehensive collection of protocols to intended to be a practical guide for the novice as well as the advanced user in the field of plant genetic transformation.
Описание: This book aims to describe, though in a quite light way, the social role of plant diseases, letting the reader know the topical importance of plant pathology, as well as the role of plant pathologists in our society.
Автор: Bhalla Prem L., Singh Mohan B. Название: Wheat Biotechnology: Methods and Protocols ISBN: 149398456X ISBN-13(EAN): 9781493984565 Издательство: Springer Рейтинг: Цена: 20962.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This detailed volume provides background on recent new technology developments highlighting the potential of the genomic era in wheat breeding with invaluable instruction on the methodology, which is complemented by overview chapters on the status of new technology application in major wheat production countries.
Автор: Wendy A. Harwood Название: Barley: Methods and Protocols ISBN: 1493989421 ISBN-13(EAN): 9781493989423 Издательство: Springer Рейтинг: Цена: 27950.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This detailed volume explores barley as both a crop and a model, with practical techniques such as crossing barley, a range of tissue culture methods, the preparation of barley tissues for different forms of microscopy, and the assessment of sensitivity to abiotic stresses. Efficient protocols are provided for transformation, TILLING, virus-induced gene silencing and genome editing. There is also particular emphasis on a range of protocols for genotyping and for the analysis of gene expression. Written for the highly successful Methods in Molecular Biology series, chapters include introductions on their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and easy-to-use, Barley: Methods and Protocols serves as a valuable reference volume for cereal researchers and breeders by providing detailed protocols covering important traditional skills such as crossing and tissue culture through to the latest technologies for genotyping, expression analysis, and genome editing.
Автор: Hicks Glenn R., Zhang Chunhua Название: Plant Chemical Genomics: Methods and Protocols ISBN: 107160953X ISBN-13(EAN): 9781071609538 Издательство: Springer Рейтинг: Цена: 18167.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This second edition volume is a companion volume to the previous edition and looks at new findings on novel bioactive chemicals and cognate targets, as well as the use of synthetic small molecules and a variety of tools to understand these processes.
Автор: Spillane Charles, McKeown Peter Название: Plant Epigenetics and Epigenomics: Methods and Protocols ISBN: 1071601784 ISBN-13(EAN): 9781071601785 Издательство: Springer Рейтинг: Цена: 16769.00 р. Наличие на складе: Поставка под заказ.
Описание: This second edition volume expands on the previous edition with a look at the latest techniques in plant epigenetics and epigenomic research. Chapters in this book cover topics such as whole genome methylome analysis; analysis of DNA methylation; chromatin analysis of metabolic gene clusters in plants; plant epigenetic stress memory induced by drought; small non-coding RNA analysis in seeds of forest tree species; and a discussion on epigenetics inheritance in plants. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Plant Epigenetics and Epigenomics: Methods and Protocols, Second Edition is a valuable resource for both novice and expert researchers, and aims to advance this field of study by paving the way for integrating epigenetic mechanisms into models of plant function during development and evolution.
Автор: Dandekar Thomas, Naseem Muhammad Название: Auxins and Cytokinins in Plant Biology: Methods and Protocols ISBN: 1493983113 ISBN-13(EAN): 9781493983117 Издательство: Springer Рейтинг: Цена: 13275.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
Preface... Table of Contents... Contributing Authors...
Methodological Advances in Auxin and Cytokinin Biology Andrej Hurnэ and Eva Benkovб
Analytical Determination of Auxins and Cytokinins Petre I. Dobrev, Klбra Hoyerovб, and Jan Petrбsek
Manipulation of Auxin and Cytokinin Balance During the Plasmodiophora brassicae: Arabidopsis thaliana Interaction Jutta Ludwig-Mьller, Susann Auer, Sabine Jьlke, and Sabine Marschollek
Microbial Manipulation of Auxin and Cytokinins in Plants Anwar Hussain, Uhsan Ullah, and Shahida Hasnain
A Standardized Method to Assess Infection Rates of Root-Knot and Cyst Nematodes in Arabidopsis thaliana Mutants with Alterations in Root Development Related to Auxin and Cytokinin Signaling Rocнo Olmo, Ana Clбudia Silva, Fernando E. Dнaz-Manzano, Javier Cabrera, Carmen Fenoll, and Carolina Escobar
Reconstruction of an Immune Dynamic Model to Simulate the Contrasting Role of Auxin and Cytokinin in Plant Immunity Martin Kaltdorf, Dandekar Thomas, and Muhammad Naseem
Interplay between Auxin and Cytokinin and its Impact on Mitogen Activated Protein Kinase (MAPK) Pallavi Singh and Alok Krishna Sinha
Quantification of Cytokinin Levels and Responses in Abiotic Stresses Alfonso Albacete
Assessment of Cytokinin-Induced Immunity through Quantification of Hyaloperonospora arabidopsidis Infection in Arabidopsis thaliana Ruth A. Watson and Cris T. Argueso
Real-Time Genetic Manipulations of the Cytokinin Pathway: A Tool for Laboratory and Field Studies Martin Schдfer and Stefan Meldau
Modulating the Levels of Plant Hormone Cytokinins at the Host-Pathogen Interface Muhammad Naseem, Shabana Shams, and Thomas Roitsch
Analyzing Cytokinin Responses during Plant-Nematode Interactions Florian M.W. Grundler and Shahid Siddique
Examining H2O2 Production in Arabidopsis Leaves Upon Challenge by Cytokinin Shahjahan Shabbir Ahmed, Ihsan Ullah, Shazia Irfan, and Nazeer Ahmed
A Systems Biology Methodology Combining Transcriptome and Interactome Datasets to Assess the Implications of Cytokinin Signaling for Plant Immune Networks Meik Kunz, Thomas Dandekar, and Muhammad Naseem
Monitoring of Crosstalk between Jasmonate and Auxin in the Framework of Plant Stress Responses of Roots Vнctor Carrasco Loba, Marta-Marina Pйrez Alonso, and Stephan Pollmann
High-Throughput Protoplast Trans-Activation (PTA) Screening to Define Transcription Factors in Auxin-Mediated Gene Regulation Nora Whner, Jцrn Herfert, Wolfgang Drцge-Laser, and Christoph Weiste
Автор: Kleine-Vehn Jьrgen, Sauer Michael Название: Plant Hormones: Methods and Protocols ISBN: 1493982109 ISBN-13(EAN): 9781493982103 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
Real Time Analysis of the Apical Hook Development.- Grafting with Arabidopsis thaliana.- Tips and Tricks for Exogenous Application of Synthetic Post-Translationally Modified Peptides to Plants.- Assaying Germination and Seedling Responses of Arabidopsis to Karrikins.- Low-cost Microprocessor-Controlled Rotating Stage for Medium-Throughput Time-Lapse Plant Phenotyping.- Genome-Wide Association Mapping of Root Traits in the Context of Plant Hormone Research.- High throughput scoring of Seed Germination.- Histochemical Staining of β-Glucuronidase and its Spatial Quantification.- Imaging TCSn:: GFP, A Synthetic Cytokinin Reporter, in Arabidopsis thaliana.- Highlighting Gibberellins Accumulation Sites in Arabidopsis Thaliana Root Using Fluorescently Labeled Gibberellins.- In-silico Methods for Cell Annotation, Quantification of Gene Expression and Cell Geometry at Single Cell Resolution using 3DCellAtlas.- Analysing Cell Wall Elasticity after Hormone Treatment: An example using Tobacco BY-2 Cells and Auzin.- FRET-FLIM for Visualizing and Quantifying Protein Interactions in Live Plant Cells.- In vivo Identification of Plant Protein Complexes using IP-MS/MS.- Assaying auxin Receptor Activity using SPR assays with F-Box Proteins and Aux-IAA degrons.- Studying Transcription Factor Binding To Specific Genomic Loci by Chromatin Immunoprecipitation (ChIP).- Hormone Receptor Glycosylation.- Highly Sensitive Salicylic Acid Quantification in Milligram Amounts of Plant Tissue.- High-Resolution Cell-Type Specific Analysis of Cytokinins in Sorted Root Cell Populations of Arabidopsis thaliana.- Hormone Profiling in Plant Tissues.- Use of Xenopus laevis Oocytes to Study Auxin Transport.- Characterizing Auxin Response Circuits in Saccharomyces cerevisiae by Flow Cytometry.
Автор: Wu Xu Na Название: Plant Phosphoproteomics: Methods and Protocols ISBN: 1071616242 ISBN-13(EAN): 9781071616246 Издательство: Springer Цена: 18167.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This detailed protocol book provides phosphoproteomics techniques currently developed for use in plants, as well as offering a variety of methodologies to analyze different types of plant phosphoproteomic data.
Автор: Bayer Martin Название: Plant Embryogenesis: Methods and Protocols ISBN: 1071603418 ISBN-13(EAN): 9781071603413 Издательство: Springer Рейтинг: Цена: 16769.00 р. Наличие на складе: Поставка под заказ.
Описание: This volume details state-of-the-art methods for the study of plant embryogenesis in the model organism Arabidopsis thaliana, other models, and non-model species.
Автор: Sambasivam Periyannan Название: Wheat Rust Diseases ISBN: 1493972480 ISBN-13(EAN): 9781493972487 Издательство: Springer Рейтинг: Цена: 13275.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
Preface... Table of Contents... Contributors...
Part I Wheat Rust Surveillance and Genotyping
1. Wheat Rust Surveillance: Field Disease Scoring and Sample Collection for Phenotyping and Molecular Genotyping Sajid Ali and David Hodson 2. Field Pathogenomics: An Advanced Tool for Wheat Rust Surveillance Vanessa Bueno-Sancho, Daniel C.E. Bunting, Luis J. Yanes, Kentaro Yoshida, and Diane G.O. Saunders 3. Race Typing of Puccinia striiformis on Wheat Mogens S. Hovm ller, Julian Rodriguez-Algaba, Tine Thach, and Chris K. S rensen 4. Assessment of Aggressiveness of Puccinina striiformis on Wheat Chris K. S rensen, Tine Thach, and Mogens S. Hovm ller 5. Extraction of High Molecular Weight DNA from Fungal Rust Spores for Long Read Sequencing Benjamin Schwessinger and John P. Rathjen 6. Microsatellite Genotyping of the Wheat Yellow Rust Pathogen Puccinia striiformis Sajid Ali, Muhammad R. Khan, Angelique Gautier, Zahoor A. Swati, and Stephanie Walter
Part II Molecular Pathogenicity 7. Computational Methods for Predicting Effectors in Rust Pathogens Jana Sperschneider, Peter N, Dodds, Jennifer M. Taylor, and Sйbastien Duplessis 8. Protein-Protein Interaction Assays with Effector-GFP Fusions in Nicotiana benthamiana Benjamin Petre, Joe Win, Frank L.H. Menke, and Sophien Kamoun 9. Proteome Profiling by 2D-Liquid Chromatography for Wheat-Rust Interaction Semra Hasanзebi 10. Investigating Gene Function in Cereal Rust Fungi by Plant-Mediated Virus-Induced Gene Silencing Vinay Panwar and Guus Bakkeren 11. Apoplastic Sugar Extraction and Quantification from Wheat Leaves Infected with Biotrophic Fungi Veronica Roman-Reyna and John P. Rathjen
Part III Genetics of Rust Resistance 12. Genetic Analysis of Resistance to Wheat Rusts Caixia Lan, Mandeep Singh Randhawa, Julio Huerta-Espino, and Ravi P. Singh 13. Advances in Identification and Mapping of Rust Resistance Genes in Wheat Urmil Bansal and Harbans Bariana 14. Chromosome Engineering Techniques for Targeted Introgression of Rust Resistance from Wild Wheat Relatives Peng Zhang, Ian S. Dundas, Steven S. Xu, Bernd Friebe, Robert A. McIntosh, and W.J. Raupp 15. Application of Genomic Selection in Breeding Wheat for Rust Resistance Leonardo Ornella, Juan Manuel Gonzбlez-Camacho, Susanne Dreisigacker, and Jose Crossa
16.Rapid Phenotyping Adult Plant Resistance to Stem Rust in Wheat Grown Under Controlled Conditions Adnan Riaz and Lee Hickey
Part IV Rust Resistance Gene Isolation and Characterization
17. Generation of Loss of Function Mutants for Wheat Rust Disease Resistance Gene Cloning Rohit Mago, Bradley Till, Sambasivam Periyannan, Guotai Yu, Brande B.H. Wulff, and Evans Lagudah 18. Isolation of Wheat Genomic DNA for Gene Mapping and Cloning Guotai Yu, Asyraf Hatta, Sambasivam Periyannan, Evans Lagudah, and Brande B.H. Wulff 19. MutRenSeq: A Method for Rapid Cloning of Plant Disease Resistance Genes Burkhard Steuernagel, Kamil Witek, Jonathan D.G. Jones, and Brande B.H. Wulff 20. Rapi
Автор: Govind Singh Saharan; Prithwi Raj Verma; Prabhu Da Название: White Rust of Crucifers: Biology, Ecology and Management ISBN: 8132217918 ISBN-13(EAN): 9788132217916 Издательство: Springer Рейтинг: Цена: 22359.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This book serves as an ideal resource for those interested in the diagnosis and management of white rust diseases of crops worldwide. It details recent advances and looks to the future of genetic engineering and manipulation.
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