Plant Hormones: Methods and Protocols, Kleine-Vehn Jьrgen, Sauer Michael
Автор: Dandekar Thomas, Naseem Muhammad Название: Auxins and Cytokinins in Plant Biology: Methods and Protocols ISBN: 1493983113 ISBN-13(EAN): 9781493983117 Издательство: Springer Рейтинг: Цена: 13275.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
Preface... Table of Contents... Contributing Authors...
Methodological Advances in Auxin and Cytokinin Biology Andrej Hurnэ and Eva Benkovб
Analytical Determination of Auxins and Cytokinins Petre I. Dobrev, Klбra Hoyerovб, and Jan Petrбsek
Manipulation of Auxin and Cytokinin Balance During the Plasmodiophora brassicae: Arabidopsis thaliana Interaction Jutta Ludwig-Mьller, Susann Auer, Sabine Jьlke, and Sabine Marschollek
Microbial Manipulation of Auxin and Cytokinins in Plants Anwar Hussain, Uhsan Ullah, and Shahida Hasnain
A Standardized Method to Assess Infection Rates of Root-Knot and Cyst Nematodes in Arabidopsis thaliana Mutants with Alterations in Root Development Related to Auxin and Cytokinin Signaling Rocнo Olmo, Ana Clбudia Silva, Fernando E. Dнaz-Manzano, Javier Cabrera, Carmen Fenoll, and Carolina Escobar
Reconstruction of an Immune Dynamic Model to Simulate the Contrasting Role of Auxin and Cytokinin in Plant Immunity Martin Kaltdorf, Dandekar Thomas, and Muhammad Naseem
Interplay between Auxin and Cytokinin and its Impact on Mitogen Activated Protein Kinase (MAPK) Pallavi Singh and Alok Krishna Sinha
Quantification of Cytokinin Levels and Responses in Abiotic Stresses Alfonso Albacete
Assessment of Cytokinin-Induced Immunity through Quantification of Hyaloperonospora arabidopsidis Infection in Arabidopsis thaliana Ruth A. Watson and Cris T. Argueso
Real-Time Genetic Manipulations of the Cytokinin Pathway: A Tool for Laboratory and Field Studies Martin Schдfer and Stefan Meldau
Modulating the Levels of Plant Hormone Cytokinins at the Host-Pathogen Interface Muhammad Naseem, Shabana Shams, and Thomas Roitsch
Analyzing Cytokinin Responses during Plant-Nematode Interactions Florian M.W. Grundler and Shahid Siddique
Examining H2O2 Production in Arabidopsis Leaves Upon Challenge by Cytokinin Shahjahan Shabbir Ahmed, Ihsan Ullah, Shazia Irfan, and Nazeer Ahmed
A Systems Biology Methodology Combining Transcriptome and Interactome Datasets to Assess the Implications of Cytokinin Signaling for Plant Immune Networks Meik Kunz, Thomas Dandekar, and Muhammad Naseem
Monitoring of Crosstalk between Jasmonate and Auxin in the Framework of Plant Stress Responses of Roots Vнctor Carrasco Loba, Marta-Marina Pйrez Alonso, and Stephan Pollmann
High-Throughput Protoplast Trans-Activation (PTA) Screening to Define Transcription Factors in Auxin-Mediated Gene Regulation Nora Whner, Jцrn Herfert, Wolfgang Drцge-Laser, and Christoph Weiste
Автор: Bolton Melvin D., Thomma Bart P. H. J. Название: Plant Fungal Pathogens: Methods and Protocols ISBN: 1493958984 ISBN-13(EAN): 9781493958986 Издательство: Springer Рейтинг: Цена: 23058.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Over the course of evolution, fungi have adapted to occupy specific niches, from symbiotically inhabiting the flora of the intestinal tract of mammals to saprophytic growth on leaf litter resting on the forest floor. In Plant Fungal Pathogens: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study fungal plant pathogens. These include methods and techniques for model systems such as Arabidopsis thaliana as well as crop plants, aspects of fungal biology, genome annotation, next-generation sequencing, and fungal transformation and molecular tools for disease and/or pathogen quantification that are critical for revealing the role for a fungal gene of interest in disease development. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Fungal Pathogens: Methods and Protocols seeks to aid scientists in the further study in current techniques that cover a wide-range of methods to study molecular aspects of pathogenesis.
Описание: 1. Elicitation Approaches for Withanolide Production in Hairy Root Culture of Withania somnifera (L.) Dunal Ganeshan Sivanandhan, Natesan Selvaraj, Andy Ganapathi, and Markandan Manickavasagan 2. Stimulant Paste Preparation and Bark Streak Tapping Technique for Pine Oleoresin Extraction Thanise Nogueira, Fьller, Jъlio Cйsar de Lima, Fernanco de Costa, Kelly C.S. Rodrigues-Corrкa, and Arthur G. Fett-Neto 3. A Modified Protocol for High Quality RNA Extraction from Oleoresin-Producing Adult Pines Jъlio Cйsar de Lima, Thanise Nogueira Fьller, Fernanda de Costa, Kelly C.S. Rodrigues-Corrкa, and Arthur G. Fett-Neto 4. Collection of Apoplastic Fluids from Arabidopsis thaliana Leaves Svend Roesen Madsen, Hussam Hassan Nour-Eldin, and Barbara Ann Halkier 5. From Plant Extract to a cDNA-Encoding a Glucosyltransferase Candidate: Proteomics and Transcriptomics as Tools to Help Elucidate Saponin Biosynthesis in Centella asiatica Fernando de Costa, Carla J.S. Barber, Darwin W. Reed, and Patrick S. Covello 6. Production of Recombinant Caffeine Synthase from Guarana (Paullinia cupana var. sorbillis) in Escherichia coli Flбvia Camila Schimpl, Eduardo Kiyota, and Paulo Mazzafera 7. Heterologous Expression and Characterization of Mimosinase from Leucaena leucocephala Vishal Singh Negi and Dulal Borthakur 8. Production of Aromatic Plant Terpenoids in Recombinant Baker's Yeast Anita Emmerstorfer and Harald Pichler 9. Purification of a Recombinant Polyhistidine-Tagged Glycosyltransferase Using Immobilized Metal-Affinity Chromatography (IMAC) Fernanda de Costa, Carla J.S. Barber, Pareshkumar T. Pujara, Darwin W. Reed, and Patrick S. Covello 10. A Western Blot Protocol for Detection of Proteins Heterologously Expressed in Xenopus laevis Oocytes Morten Egevang Jrgensen, Hussam Hassan Nour-Eldin, and Barbara Ann Halkier 11. Laser Capture Microdissection: Avoiding Bias in Analysis by Selecting Just What Matters Mбrcia R. de Almeida and Martina V. Strцmvik
12. Analytical and Fluorimetric Methods for the Characterization of the Transmembrane Transport of Specialized Metabolites in Plants Inкs Carqueijeiro, Viviana Martins, Henrique Noronha, Hernвni Gerуs, and Mariana Sottomayor 13. Protoplast Transformation as a Plant-Transferable Transient Expression System Patrнcia Duarte, Diana Ribeiro, Inкs Carqueijeiro, Sara Bettencourt, and Mariana Sottomayor
14. Design, Construction, and Validation of Artificial MicroRNA Vectors Using Agrobacterium-Mediated Transient Expression System Basdeo Bhagwat, Ming Chi, Dianwei Han, Haifeng Tang, Guiliang Tang, and Yu Xiang
15. Knockdown of Polyphenol Oxidase Gene Expression in Potato (Solanum tuberosum L.) with Artificial MicroRNAs Ming Chi, Basdeo Bhagwat, Guiliang Tang, and Yu Xiang
Автор: Kianian Shahryar F., Kianian Penny M. Avoles Название: Plant Cytogenetics: Methods and Protocols ISBN: 1493980971 ISBN-13(EAN): 9781493980970 Издательство: Springer Рейтинг: Цена: 15902.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume covers arange of methods used in plant cytogenetics, beginning with basic analysis ofchromosomes and visualizing gene locations, to manipulating and dissectingchromosomes, and then focusing on less understood features of chromosomes suchas recombination initiation sites and epigenomic marks.
Автор: Kaufmann Kerstin, Mueller-Roeber Bernd Название: Plant Gene Regulatory Networks: Methods and Protocols ISBN: 149398392X ISBN-13(EAN): 9781493983926 Издательство: Springer Рейтинг: Цена: 22359.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
Preface... Table of Contents...
Contributing Authors...
1. From Genes to Networks: Characterizing Gene-Regulatory Interactions in Plants
Kerstin Kaufmann and Dijun Chen
Part I Experimental Approaches to Study Plant Gene-Regulatory Networks
2. Inducible Promoter Systems for Gene Perturbation Experiments in Arabidopsis Bennett Thomson, Emmanuelle Graciet, and Frank Wellmer
3. Cell Type-Specific Gene Expression Profiling Using Fluorescence-Activated Nuclear Sorting Daniel Slane and Martin Bayer
4. Characterization of Cell Type-Specific DNA Binding Sites of Plant Transcription Factors using Chromatin Immunoprecipitation On Sun Lau
5. Yeast One and Two Hybrid High-Throughput Screenings using Arrayed Libraries Rocнo Sбnchez-Montesino and Luis Oсate-Sбnchez
6.SELEX-Seq: A Method to Determine DNA-Binding Specificities of Plant Transcription Factors Cezary Smaczniak, Gerco C. Angenent and Kerstin Kaufmann
7. Analysis of a Plant Transcriptional Regulatory Network using Transient Expression Systems Sara Dнaz-Triviсo, Yuchen Long, Ben Scheres, and Ikram Blilou
8.Analysis of In Vivo Chromatin and Protein Interactions of Arabidopsis Transcript Elongation Factors Alexander Pfab, Wojciech Antosz, Philipp Holzinger, Astrid Bruckmann, Joachim Griesenbeck, and Klaus D. Grasser
9.Characterization of Mediator Complex and its Associated Proteins from Rice Subhasis Samanta and Jitendra Kumar Thakur
10. DNase I SIM: A Simplified In-Nucleus Method for DNase I Hypersensitive Site Sequencing Sergei A. Filichkin and Molly Megraw
11. In Situ Hi-C Library Preparation for Plants to Study their Three-Dimensional Chromatin Interactions on a Genome-Wide Scale Chang Liu
12. Multiplexed Transcriptional Activation or Repression in Plants using CRISPR-dCas9 Based Systems Levi G. Lowder, Joseph W. Paul III, and Yiping Qi
13. Generation of dTALEs and Libraries of Synthetic TALE-Activated Promoters for Engineering of Gene Regulatory Networks in Plants Tom Schreiber and Alain Tissier
Part II Computational Approaches to Study Plant Gene-Regulatory Networks
14.Design of Knowledge Bases for Plant Gene Regulatory Networks Eric Mukundi, Fabio Gomez-Cano, Wilberforce Zachary Ouma, and Erich Grotewold
15. AraNet: A Network Biology Server for Arabidopsis thaliana and Other Non-Model Plant Species Tak Lee and Insuk Lee
16. Integration of Genome-Wide TF Binding and Gene Expression Data to Characterize Gene Regulatory Networks in Plant Development Dijun Chen and Kerstin Kaufmann
17. Predicting Transcription Factor-Binding Sites and their Cognate Transcription Factors using Gene Expression Data Chun-Ping Yu and Wen-Hsiung Li
18. Computational Approaches to Study Gene Regulatory Networks Nooshin Omranian and Zoran Nikoloski
19. Boolean Dynamic Modeling Approaches to Study Plant Gene Regulatory Networks: Integration, Validation and Prediction J. Davila-Velderrain, J.C. Martinez-Garcia, and R.E. Alvarez Buylla
20. ODE-Based Modeling of Complex Regulatory Circuits Daniel D. Seaton
21. Inferring Gene Regulatory Networks in the Arabidopsis Root using a Dynamic Bayesian Network Approach Maria Ang
Описание: This volume provides readers with wide-ranging coverage of CRISPR systems and their applications in various plant species. The chapters in this book discuss topics such as plant DNA repair and genome editing; analysis of CRISPR-induced mutations; multiplexed CRISPR/Cas9 systems; CRISPR-Cas12a (Cpf1) editing systems; and non-agrobacterium based CRISPR delivery systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Comprehensive and thorough, Plant Genome Editing with CRISPR Systems: Methods and Protocols is a valuable resource for any researcher interested in learning about and using CRISPR systems in plants.
Описание: The orchid family is one of the largest families of flowering plants known for their beauty and economic importance. This work provides information in key areas of research that are important to both scientists and commercial growers alike. The main purposes of this book are to provide key practical areas of research, such as, germination, micropropagation, traditional and current techniques related to plant improvement; document methods that ensure survival of plants from laboratories to greenhouses; promote communication between scientists and growers, so that their combined expertise on these areas will lead to the successful growth of orchids in their natural habitats or commercial greenhouses. This book can serve as reference for laymen with an interest in orchid growing.
This book is divided into 5 parts. The first part emphasizes propagation methods using seeds and related techniques that are important to plant conservation and improvement. Successes in asymbiotic and symbiotic seed germination are keys to orchid conservation and their propagation. The second part summarizes micropropagation methods, common media, and newer methods of micropropagation such as the bioreactor culture procedures. The third part focuses on techniques related to the manipulation of explants in an in vitro environment. The fourth part covers cell biological methods and transformation techniques. Since the successes in a laboratory setting do not guarantee plant survival and propagation in greenhouses and in the natural environment, it discusses greenhouse propagation techniques that are essential to the survival of plants generated from a laboratory setting. The fifth part showcases recent successes on orchid propagation by documenting sample publications and how to present orchids in an artistic fashion for one’s enjoyment.
Автор: Van Dijk Aalt D. J. Название: Plant Genomics Databases: Methods and Protocols ISBN: 149398263X ISBN-13(EAN): 9781493982639 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
Preface... Table of Contents... Contributing Authors...
1. Ensembl Plants: Integrating Tools for Visualizing, Mining and Analyzing Plant Genomics Data Dan M. Bolser, Daniel M. Staines, Emily Perry, and Paul J. Kersey
2. PGSB/MIPS PlantsDB Database Framework for the Integration and Analysis of Plant Genome Data Manuel Spannagl, Thomas Nussbaumer, Kai Bader, Heidrun Gundlach, and Klaus FX Mayer 3. Plant Genome DataBase Japan (PGDBj) Akihiro Nakaya, Hisako Ichihara, Erika Asamizu, Sachiko Shirasawa, Yasukazu Makamura, Satoshi Tabata, and Hideki Hirakawa 4. FLAGdb++: A Bioinformatic Environment to Study and Compare Plant Genomes Jean Philippe Tamby and Vйronique Brunaud 5. Mining Plant Genomic and Genetic Data Using the GnpIS Information System A-F Adam-Blondon, M. Alaux, S. Durand, T. Letellier, G. Merceron, N. Mohellibi, C. Pommier, D. Steinbach, F. Alfama, J. Amselem, D. Charruaud, N. Choisne, R. Flores, C. Guerche, V. Jamilloux, E. Kimmel, N. Lapalu, M. Loaec, C. Michotey, H. Quesneville 6. The Bio-Analytic Resource for Plant Biology Jamie Waese and Nicholas J. Provart 7. The Evolution of Soybean Knowledge Base (SoyKB) Trupti Joshi, Jiaojiao Wang, Hongxin Zhang, Shiyuan Chen, Shuai Zeng, Bowei Xu, and Dong Xu 8. Using TropGeneDB: a Database Containing Data on Molecular Markers, QTLs, Maps, Genotypes, and Phenotypes for Tropical Crops Manuel Ruiz, Guilhem Sempere, and Chantal Hamelin 9. Species-Specific Genome Sequence Databases: A Practical Review A.D.J. van Dijk 10. A Guide to the PLAZA 3.0 Plant Comparative Genomics Database Klaas Vandepoele 11. Exploring Plant Co-Expression and Gene-Gene Interactions with CORNET 3.0 Michiel Van Bel and Frederik Coppens 12. PlaNet: Comparative Co-Expression Network Analyses for Plants Sebastian Proost and Marek Mutwil 13. Practical Utilization of OryzaExpress and Plant Omics Data Center Databases to Explore Gene Expression Networks in Oryza sativa and Other Plant Species Toru Kudo, Shin Terashima, Yuno Takaki, Yukino Nakamura, Masaaki Kobayashi, and Kentaro Yano 14. Pathway Analysis and Omics Data Visualization using Pathway Genome Databases: FragariaCyc, A Case Study Sushma Naithani and Pankaj Jaiswal 15. CSGRqtl: A Comparative Quantitative Trait Locus Database for Saccharinae Grasses Dong Zhang and Andrew H. Paterson 16. Plant Genome Duplication Database Tae-Ho Lee, Junah Kim, Jon S. Robertson, and Andrew H. Paterson 17. Variant Effect Prediction Analysis using Resources Available at Gramene Database Sushma Naithani, Matt Geniza, and Pankaj Jaiswal 18. Plant Promoter Database Kazutaka Kusunoki and Yoshiharu Y. Yamamoto 19. Construction of the Leaf Senescence Database and Functional Assessment of Senescence-Associated Genes Zhonghai Li, Yi Zhao, Xiaochuan Liu, Zhiqiang Jiang, Jinying Peng, Jinpu Jin, Hongwei Guo, and Jingchu Luo
Автор: Kovalchuk Igor Название: Plant Epigenetics: Methods and Protocols ISBN: 1493979566 ISBN-13(EAN): 9781493979561 Издательство: Springer Рейтинг: Цена: 13275.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
1. Chromatin Immunoprecipitation Protocol for Histone Modifications and Protein-DNA Binding Analyses in Arabidopsis
Wanhui You, Stйphane Pien, and Ueli Grossniklaus
2. Chromatin Conformation Capture-Based Analysis of Nuclear Architecture
Stefan Grob and Ueli Grossniklaus
3. Metaanalysis of Genome-Wide Chromatin Data
Julia Engelhorn and Franziska Turck
4. Localization of Mirnas by In Situ Hybridization in Plants using Conventional Oligonucleotide Probes
Sara Hernбndez-Castellano, Geovanny I. Nic-Can, Clelia De-La-Peсa
5. COBRA (Combined Bisulfite Restriction Analysis) Assay for the Analysis of Locus-Specific Changes in Methylation Patterns
Andriy Bilichak and Igor Kovalchuk
6. Analysis of Global Genome Methylation Using the Cytosine-Extension Assay
Andriy Bilichak and Igor Kovalchuk
7. In Situ Analysis Of DNA Methylation in Plants
Palak Kathiria and Igor Kovalchuk
8. Analysis of DNA Hydroxymethylation in Arabidopsis Using Colorimetric Assay
Andrey Golubov and Igor Kovalchuk
9. Analysis of DNA Cytosine Methylation Patterns using Methylation Specific Amplification Polymorphism (MSAP)
Marнa Бngeles Guevara, Nuria De Marнa, Enrique Sбez-Laguna, Marнa Dolores Vйlez, Marнa Teresa Cervera, and Josй Antonio Cabezas
10. Differentially Methylated Region - Representational Difference Analysis (DMR-RDA): A Powerful Method to Identify Dmrs in Uncharacterized Genomes
Pavlina Sasheva Ueli Grossniklaus
11. Analysis of Small RNA Populations Using Hybridization to DNA Tiling Arrays
Martine Boccara, Alexis Sarazin, Bernard Billoud, Agnes Bulski, Louise Chapell, David Baulcombe, and Vincent Colot
12. Northern Blotting Techniques for Small RNAs
Todd Blevins
13. Stem-Loop QRT-PCR for the Detection of Plant Micrornas
Erika Varkonyi-Gasic
14. Profiling New Small RNA Sequences
Masayuki Tsuzuki and Yuichiro Watanabe
15. Small RNA Library Preparation and Illumina Sequencing in Plants
Andriy Bilichak, Andrey Golubov, and Igor Kovalchuk
16. Bioinformatics Analysis of Small RNA Transcriptomes - The Detailed Work Flow
Slava Ilnytskyy and Andriy Bilichak
17. Increasing a Stable Transformation Efficiency of Arabidopsis by Manipulating the Endogenous Gene Expression using Virus-Induced Gene Silencing
Andriy Bilichak and Igor Kovalchuk
18. The Random Oligonucleotide-Primed Synthesis Assay for the Quantification of DNA Strand Breaks
Andriy Bilichak and Igor Kovalchuk
19. Profiling Transposable Elements and their Epigenetic Effects in Non-Model Species
Christian Parisod
Автор: Bayer Martin Название: Plant Embryogenesis: Methods and Protocols ISBN: 1071603418 ISBN-13(EAN): 9781071603413 Издательство: Springer Рейтинг: Цена: 16769.00 р. Наличие на складе: Поставка под заказ.
Описание: This volume details state-of-the-art methods for the study of plant embryogenesis in the model organism Arabidopsis thaliana, other models, and non-model species.
Автор: Dissmeyer Nico, Schnittger Arp Название: Plant Kinases: Methods and Protocols ISBN: 149395721X ISBN-13(EAN): 9781493957217 Издательство: Springer Рейтинг: Цена: 16769.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Modification of target protein properties by reversible phosphorylation events has been found to be one of the most prominent cellular control processes in all organisms. Recent advances in the areas of molecular biology and biochemistry are presenting new possibilities for reaching an unprecedented depth and a proteome-wide understanding of phosphorylation processes in plants as well as in other species. The major goal of Plant Kinases: Methods and Protocols is to provide the experimentalist with a detailed account of the practical steps necessary for successfully carrying out each protocol in his or her own laboratory. Plant protein kinases specifically addressed in this volume are members of the plant MAP kinase cascade, cyclin- and Calcium-dependent protein kinases, and plant sensor and receptor kinases. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Plant Kinases: Methods and Protocols will prove a useful laboratory companion to both novice and seasoned researchers by facilitating the practical work that will lead them to new and exciting insights in this dynamic field.
Автор: Spillane Charles, McKeown Peter Название: Plant Epigenetics and Epigenomics: Methods and Protocols ISBN: 1071601784 ISBN-13(EAN): 9781071601785 Издательство: Springer Рейтинг: Цена: 16769.00 р. Наличие на складе: Поставка под заказ.
Описание: This second edition volume expands on the previous edition with a look at the latest techniques in plant epigenetics and epigenomic research. Chapters in this book cover topics such as whole genome methylome analysis; analysis of DNA methylation; chromatin analysis of metabolic gene clusters in plants; plant epigenetic stress memory induced by drought; small non-coding RNA analysis in seeds of forest tree species; and a discussion on epigenetics inheritance in plants. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Plant Epigenetics and Epigenomics: Methods and Protocols, Second Edition is a valuable resource for both novice and expert researchers, and aims to advance this field of study by paving the way for integrating epigenetic mechanisms into models of plant function during development and evolution.
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