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Plant Kinases: Methods and Protocols, Dissmeyer Nico, Schnittger Arp


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Автор: Dissmeyer Nico, Schnittger Arp
Название:  Plant Kinases: Methods and Protocols
ISBN: 9781493957217
Издательство: Springer
Классификация:

ISBN-10: 149395721X
Обложка/Формат: Paperback
Страницы: 312
Вес: 0.56 кг.
Дата издания: 23.08.2016
Серия: Methods in molecular biology
Язык: English
Издание: Softcover reprint of
Иллюстрации: 15 tables, black and white; x, 312 p.
Размер: 25.40 x 17.78 x 1.73 cm
Читательская аудитория: General (us: trade)
Подзаголовок: Methods and protocols
Ссылка на Издательство: Link
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Поставляется из: Германии
Описание: Modification of target protein properties by reversible phosphorylation events has been found to be one of the most prominent cellular control processes in all organisms. Recent advances in the areas of molecular biology and biochemistry are presenting new possibilities for reaching an unprecedented depth and a proteome-wide understanding of phosphorylation processes in plants as well as in other species. The major goal of Plant Kinases: Methods and Protocols is to provide the experimentalist with a detailed account of the practical steps necessary for successfully carrying out each protocol in his or her own laboratory. Plant protein kinases specifically addressed in this volume are members of the plant MAP kinase cascade, cyclin- and Calcium-dependent protein kinases, and plant sensor and receptor kinases. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Plant Kinases: Methods and Protocols will prove a useful laboratory companion to both novice and seasoned researchers by facilitating the practical work that will lead them to new and exciting insights in this dynamic field.
Дополнительное описание: Guide to the Book Plant Kinases.- The Age of Protein Kinases.- Expression and Purification of Active Protein Kinases from Wheat Germ Extracts.- Measurement of Plant Cyclin-Dependent Kinase Activity Using Immunoprecipitation-Coupled and Affinity Purificati



Plant Receptor Kinases: Methods and Protocols

Автор: Aalen Reidunn Birgitta
Название: Plant Receptor Kinases: Methods and Protocols
ISBN: 1493983822 ISBN-13(EAN): 9781493983827
Издательство: Springer
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Цена: 18167.00 р.
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Описание: Chapters guide readers through comprehensive experimental approach for molecular investigations of plant receptor kinases, from the simplest methods for expression and purification of receptor domains to the most advanced methods aiming at understanding the dynamics of receptor complex formation and specificity of signaling pathways.

MAP Kinases in Plant Signal Transduction

Автор: Heribert Hirt
Название: MAP Kinases in Plant Signal Transduction
ISBN: 3642536921 ISBN-13(EAN): 9783642536922
Издательство: Springer
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Цена: 13974.00 р.
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Plant Receptor Kinases

Автор: Reidunn Birgitta Aalen
Название: Plant Receptor Kinases
ISBN: 1493970623 ISBN-13(EAN): 9781493970629
Издательство: Springer
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Цена: 19564.00 р.
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Описание: Presenting protocols for plant receptor kinases (PRK) in development and defence, this book guides through experimental approaches for molecular investigations, from the simplest methods for expression and purification of receptor domains to advanced methods for understanding receptor complex formation and specificity of signaling pathways.

Receptor Tyrosine Kinases

Автор: Serena Germano
Название: Receptor Tyrosine Kinases
ISBN: 1493954490 ISBN-13(EAN): 9781493954490
Издательство: Springer
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Цена: 13275.00 р.
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Описание:

Part I: RTKs Activation and Signalling

1. Analysis of Receptor Tyrosine Kinase (RTK) Phosphorylation by Immunoblotting

Martina McDermott and Norma O'Donovan

2. Analysis of Changes in Phosphorylation of Receptor Tyrosine Kinases: Antibody Arrays

Sweta Rani and Lorraine O'Driscoll

3. Analysis of Epidermal Growth Factor Receptor Dimerization by BS3 Crosslinking

Harmony F. Turk and Robert S. Chapkin

4. Single-Molecule Optical Methods Analyzing Receptor Tyrosine Kinase Activation in Living Cells

Inhee Chung and Ira Mellman

5. Evaluation of the Dimerization Profiles of HER Tyrosine Kinases by Time-Resolved Fцrster Resonance Energy Transfer (TR-FRET)

Evelyne Lopez-Crapez, Alexandre Ho-Pun-Cheung, Patrick Garnero, and Hervй Bazin

6. Applying the Proximity Ligation Assay (PLA) to Mouse Preimplantation Embryos for Identifying Protein-Protein Interactions In Situ

Ivan Bedzhov and Marc P. Stemmler

Part II: RTKs Aberrant Expression

7. Analysis of Receptor Tyrosine Kinase Gene Amplification on the Example of FGFR1

Diana Boehm, Anne von Mдssenhausen, and Sven Perner

8. Quantification of the Effects of Mutations on Receptor Tyrosine Kinase (RTK) Activation in Mammalian Cells

Lijuan He and Kalina Hristova

Part III: RTKs Trafficking and Negative Regulation

9. Cell Surface Biotinylation of Receptor Tyrosine Kinases to Investigate Intracellular Trafficking

Mathieu J.F. Crupi, Douglas S. Richardson, and Lois M. Mulligan

10. Studying N-Linked Glycosylation of Receptor Tyrosine Kinases

Harri M. Itkonen and Ian G. Mills

11. Identification of Receptor Protein Tyrosine Phosphatases (RPTPs) as Regulators of Receptor Tyrosine Kinases (RTKs) Using an RPTP siRNA-RTK Substrate Screen

Hojin Lee and Anton M. Bennett

12. Downregulation of Receptor Tyrosine Kinases Through Ubiquitination: Analysis by Immunodetection

Noriaki Shimokawa and Noriyuki Koibuchi

13. Regulation of Receptor Tyrosine Kinases by miRNA: Overexpression of miRNA Using Lentiviral Inducible Expression Vectors

XiangDong Le, Andrew T. Huang, Yunyun Chen, and Stephen Y. Lai

Part IV: RTKs as Pharmacological Targets

14. Human Tumor Xenografts in Mouse as a Model for Evaluating Therapeutic Efficacy of Monoclonal Antibodies or Antibody-Drug Conjugate Targeting Receptor Tyrosine Kinases

Liang Feng, Wei Wang, Hang-Ping Yao, Jianwei Zhou, Ruiwen Zhang, and Ming-Hai Wang

15. Receptor Tyrosine Kinase Targeting in Multicellular Spheroids

Susan Breslin and Lorraine O'Driscoll

16. Receptor Tyrosine Kinases and Drug Resistance: Development and Characterization of In Vitro Models of Resistance to RTK Inhibitors

Claire Corcoran and Lorraine O'Driscoll

Role of Tyrosine Kinases in Gastrointestinal Malignancies

Автор: Ganji Purnachandra Nagaraju
Название: Role of Tyrosine Kinases in Gastrointestinal Malignancies
ISBN: 9811346402 ISBN-13(EAN): 9789811346408
Издательство: Springer
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Цена: 19564.00 р.
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Описание: The aim of this book is to provide comprehensive overview of the role of different tyrosine kinases in the progression and metastasis of various cancers of the gastrointestinal tract such as esophagus, liver, pancreas, stomach, and colorectal. Activation of various signaling pathways and tyrosine kinases are responsible for resistance to chemo and radiotherapy in these gastrointestinal malignancies. Targeting these tyrosine kinases, which regulate the activities of survival pathways in growth, and metastasis is a rational strategy in gastrointestinal cancer therapy.Each chapter embedded in this book covers information pertaining to a specific tyrosine kinase and its impact on various malignancies, which is not only significant for basic and clinical research investigations but will also be valued by students at advanced undergraduate to postgraduate levels.

Stress-Activated Protein Kinases

Автор: Francesc Posas; Angel R. Nebreda
Название: Stress-Activated Protein Kinases
ISBN: 3540755683 ISBN-13(EAN): 9783540755685
Издательство: Springer
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Цена: 40389.00 р.
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Описание: Stress stimuli result in the rapid activation of a highly conserved group of MAP kinases, known as SAPKs (Stress-Activated Protein Kinases). These kinases coordinate the generation of adaptive responses that are essential for cell survival. This book discusses many aspects of SAPK signalling in various systems from yeast to mammals.

Receptor Tyrosine Kinases: Structure, Functions and Role in Human Disease

Автор: Deric L. Wheeler; Yosef Yarden
Название: Receptor Tyrosine Kinases: Structure, Functions and Role in Human Disease
ISBN: 1493920529 ISBN-13(EAN): 9781493920525
Издательство: Springer
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Цена: 22203.00 р.
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Описание: Receptor Tyrosine Kinase: Structure, Functions and Role in Human Disease, for the first time, systematically covers the shared structural and functional features of the RTK family.

Receptor-like Kinases in Plants

Автор: Frans Tax; Birgit Kemmerling
Название: Receptor-like Kinases in Plants
ISBN: 3642447988 ISBN-13(EAN): 9783642447983
Издательство: Springer
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Цена: 32004.00 р.
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Описание: Revealed by sequencing projects, receptor kinases are the largest group of primary signal transducers in plants. Their abundance hints at an immense network that we have just begun to reveal. This volume focuses on their role, signaling paths, and regulation.

Plant Synthetic Promoters: Methods and Protocols

Автор: Hehl Reinhard
Название: Plant Synthetic Promoters: Methods and Protocols
ISBN: 1493981862 ISBN-13(EAN): 9781493981861
Издательство: Springer
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Цена: 15372.00 р.
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Описание: Practical and cutting-edge, Plant Synthetic Promoters: Methods and Protocols covers all steps required from the identification of cis-regulatory elements, over synthetic promoter design, to the experimental analysis of synthetic promoter function.

Plant Pattern Recognition Receptors: Methods and Protocols

Автор: Shan Libo, He Ping
Название: Plant Pattern Recognition Receptors: Methods and Protocols
ISBN: 1493983202 ISBN-13(EAN): 9781493983209
Издательство: Springer
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Цена: 20962.00 р.
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Описание: This volume covers protocols on techniques ranging from MAMP isolations from diverse microorganisms, PRR identifications from different plant species, MAMP-PRR binding, and a series of signaling responses and events revealed by various biochemical, cellular, genetic and bioinformatic tools.

Biotechnology of Plant Secondary Metabolism: Methods and Protocols

Автор: Fett-Neto Arthur Germano
Название: Biotechnology of Plant Secondary Metabolism: Methods and Protocols
ISBN: 1493980408 ISBN-13(EAN): 9781493980406
Издательство: Springer
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Цена: 15902.00 р.
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Описание: 1. Elicitation Approaches for Withanolide Production in Hairy Root Culture of Withania somnifera (L.) Dunal
Ganeshan Sivanandhan, Natesan Selvaraj, Andy Ganapathi, and Markandan Manickavasagan
2. Stimulant Paste Preparation and Bark Streak Tapping Technique for Pine Oleoresin Extraction
Thanise Nogueira, Fьller, Jъlio Cйsar de Lima, Fernanco de Costa, Kelly C.S. Rodrigues-Corrкa, and Arthur G. Fett-Neto
3. A Modified Protocol for High Quality RNA Extraction from Oleoresin-Producing Adult Pines
Jъlio Cйsar de Lima, Thanise Nogueira Fьller, Fernanda de Costa, Kelly C.S. Rodrigues-Corrкa, and Arthur G. Fett-Neto

4. Collection of Apoplastic Fluids from Arabidopsis thaliana Leaves
Svend Roesen Madsen, Hussam Hassan Nour-Eldin, and Barbara Ann Halkier

5. From Plant Extract to a cDNA-Encoding a Glucosyltransferase Candidate: Proteomics and Transcriptomics as Tools to Help Elucidate Saponin Biosynthesis in Centella asiatica
Fernando de Costa, Carla J.S. Barber, Darwin W. Reed, and Patrick S. Covello

6. Production of Recombinant Caffeine Synthase from Guarana (Paullinia cupana var. sorbillis) in Escherichia coli
Flбvia Camila Schimpl, Eduardo Kiyota, and Paulo Mazzafera

7. Heterologous Expression and Characterization of Mimosinase from Leucaena leucocephala
Vishal Singh Negi and Dulal Borthakur

8. Production of Aromatic Plant Terpenoids in Recombinant Baker's Yeast
Anita Emmerstorfer and Harald Pichler

9. Purification of a Recombinant Polyhistidine-Tagged Glycosyltransferase Using Immobilized Metal-Affinity Chromatography (IMAC)
Fernanda de Costa, Carla J.S. Barber, Pareshkumar T. Pujara, Darwin W. Reed, and Patrick S. Covello

10. A Western Blot Protocol for Detection of Proteins Heterologously Expressed in Xenopus laevis Oocytes
Morten Egevang J rgensen, Hussam Hassan Nour-Eldin, and Barbara Ann Halkier

11. Laser Capture Microdissection: Avoiding Bias in Analysis by Selecting Just What Matters
Mбrcia R. de Almeida and Martina V. Strцmvik

12. Analytical and Fluorimetric Methods for the Characterization of the Transmembrane Transport of Specialized Metabolites in Plants
Inкs Carqueijeiro, Viviana Martins, Henrique Noronha, Hernвni Gerуs, and Mariana Sottomayor

13. Protoplast Transformation as a Plant-Transferable Transient Expression System
Patrнcia Duarte, Diana Ribeiro, Inкs Carqueijeiro, Sara Bettencourt, and Mariana Sottomayor

14. Design, Construction, and Validation of Artificial MicroRNA Vectors Using Agrobacterium-Mediated Transient Expression System
Basdeo Bhagwat, Ming Chi, Dianwei Han, Haifeng Tang, Guiliang Tang, and Yu Xiang

15. Knockdown of Polyphenol Oxidase Gene Expression in Potato (Solanum tuberosum L.) with Artificial MicroRNAs
Ming Chi, Basdeo Bhagwat, Guiliang Tang, and Yu Xiang


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