Plant Pattern Recognition Receptors: Methods and Protocols, Shan Libo, He Ping
Автор: Dissmeyer Nico, Schnittger Arp Название: Plant Kinases: Methods and Protocols ISBN: 149395721X ISBN-13(EAN): 9781493957217 Издательство: Springer Рейтинг: Цена: 16769.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Modification of target protein properties by reversible phosphorylation events has been found to be one of the most prominent cellular control processes in all organisms. Recent advances in the areas of molecular biology and biochemistry are presenting new possibilities for reaching an unprecedented depth and a proteome-wide understanding of phosphorylation processes in plants as well as in other species. The major goal of Plant Kinases: Methods and Protocols is to provide the experimentalist with a detailed account of the practical steps necessary for successfully carrying out each protocol in his or her own laboratory. Plant protein kinases specifically addressed in this volume are members of the plant MAP kinase cascade, cyclin- and Calcium-dependent protein kinases, and plant sensor and receptor kinases. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Plant Kinases: Methods and Protocols will prove a useful laboratory companion to both novice and seasoned researchers by facilitating the practical work that will lead them to new and exciting insights in this dynamic field.
Автор: Spillane Charles, McKeown Peter Название: Plant Epigenetics and Epigenomics: Methods and Protocols ISBN: 1071601784 ISBN-13(EAN): 9781071601785 Издательство: Springer Рейтинг: Цена: 16769.00 р. Наличие на складе: Нет в наличии.
Описание: This second edition volume expands on the previous edition with a look at the latest techniques in plant epigenetics and epigenomic research. Chapters in this book cover topics such as whole genome methylome analysis; analysis of DNA methylation; chromatin analysis of metabolic gene clusters in plants; plant epigenetic stress memory induced by drought; small non-coding RNA analysis in seeds of forest tree species; and a discussion on epigenetics inheritance in plants. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Plant Epigenetics and Epigenomics: Methods and Protocols, Second Edition is a valuable resource for both novice and expert researchers, and aims to advance this field of study by paving the way for integrating epigenetic mechanisms into models of plant function during development and evolution.
Автор: Gupta Kapuganti Jagadis Название: Plant Nitric Oxide: Methods and Protocols ISBN: 1493980904 ISBN-13(EAN): 9781493980901 Издательство: Springer Рейтинг: Цена: 6986.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: With numerous methods available for the quantification ofnitric oxide (NO), this detailed book explores their advantages anddisadvantages in order to provide a foundation for further research in plantnitric oxide.
Описание: The orchid family is one of the largest families of flowering plants known for their beauty and economic importance. This work provides information in key areas of research that are important to both scientists and commercial growers alike. The main purposes of this book are to provide key practical areas of research, such as, germination, micropropagation, traditional and current techniques related to plant improvement; document methods that ensure survival of plants from laboratories to greenhouses; promote communication between scientists and growers, so that their combined expertise on these areas will lead to the successful growth of orchids in their natural habitats or commercial greenhouses. This book can serve as reference for laymen with an interest in orchid growing.
This book is divided into 5 parts. The first part emphasizes propagation methods using seeds and related techniques that are important to plant conservation and improvement. Successes in asymbiotic and symbiotic seed germination are keys to orchid conservation and their propagation. The second part summarizes micropropagation methods, common media, and newer methods of micropropagation such as the bioreactor culture procedures. The third part focuses on techniques related to the manipulation of explants in an in vitro environment. The fourth part covers cell biological methods and transformation techniques. Since the successes in a laboratory setting do not guarantee plant survival and propagation in greenhouses and in the natural environment, it discusses greenhouse propagation techniques that are essential to the survival of plants generated from a laboratory setting. The fifth part showcases recent successes on orchid propagation by documenting sample publications and how to present orchids in an artistic fashion for one’s enjoyment.
Автор: Aalen Reidunn Birgitta Название: Plant Receptor Kinases: Methods and Protocols ISBN: 1493983822 ISBN-13(EAN): 9781493983827 Издательство: Springer Рейтинг: Цена: 18167.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Chapters guide readers through comprehensive experimental approach for molecular investigations of plant receptor kinases, from the simplest methods for expression and purification of receptor domains to the most advanced methods aiming at understanding the dynamics of receptor complex formation and specificity of signaling pathways.
Автор: Busch Wolfgang Название: Plant Genomics: Methods and Protocols ISBN: 1493983644 ISBN-13(EAN): 9781493983643 Издательство: Springer Рейтинг: Цена: 19564.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Part I: Genotypes
1. CRISPR/Cas-mediated in planta Gene Targeting
Simon Schiml, Friedrich Fauser, and Holger Puchta
2. User Guide for the LORE1 Insertion Mutant Resource
Terry Mun, Anna Malolepszy, Niels Sandal, Jens Stougaard, and Stig U. Andersen
3. Enabling Reverse Genetics in Medicago truncatula Using High Throughput Sequencing for Tnt1 Flanking Sequence Recovery
Xiaofei Cheng, Nick Krom, Shulan Zhang, Kirankumar S. Mysore, Michael Udvardi, and Jiangqi Wen
4. Generation of Doubled Haploid Lines for QTL Mapping
Daniele L. Filiault, Danelle K. Seymour, Ravi Maruthachalam, and Julin N. Maloof
Part II: Phenotypes
5. Assessing Distribution and Variation of Genome-wide DNA Methylation using Short Read Sequencing
Jцrg Hagmann and Claude Becker
6.Circular Chromosome Conformation Capture in Plants
Stefan Grob
7. Genome Wide Profiling of Histone Modifications and Histone Variants in Arabidopsis thaliana and Marchantia polymorpha
8. Tissue-Specific Transcriptome Profiling in Arabidopsis Roots
Erin E. Sparks and Philip N. Benfey
9. Sample Preparation Protocols for Protein Abundance, Acetylome and Phosphoproteome Profiling of Plant Tissues
Gaoyuan Song, Maxwell R McReynolds, and Justin W Walley
10. Automated High-throughput Root Phenotyping of Arabidopsis thaliana under Nutrient Deficiency Conditions
Santosh B. Satbhai, Christian Göschl, and Wolfgang Busch
11. Large-scale Phenotyping of Root Traits in the Model Legume Lotus japonicus
Marco Giovannetti, Anna Malolepszy, Christian Goeschl, and Wolfgang Busch
12. Long-term Confocal Imaging of Arabidopsis thaliana Roots for Simultaneous Quantification of Root Growth and Fluorescent Signals
Delyana Stoeva, Christian Gцschl, and Wolfgang Busch
Part III: Molecular Bases of Phenotypes
13. Identification of Protein-DNA Interactions Using Enhanced Yeast One-Hybrid Assays and a Semi-Automated Approach
Allison Gaudinier, Michelle Tang, Anne-Maarit Bеgman, and Siobhan M. Brady
14. Mapping Protein- protein Interaction using High-throughput Yeast-2-Hybrid
Jessica Lopez and M. Shahid Mukhtar
15.Mapping Protein-protein Interactions using Affinity Purification and Mass Spectrometry
Chin-Mei Lee, Christopher Adamchek, Ann Feke, Dmitri A. Nusinow, and Joshua M. Gendron
16. Measuring Protein Movement, Oligomerization State, and Protein-Protein Interaction in Arabidopsis Roots Using Scanning Fluorescence Correlation Spectroscopy (scanning FCS) Natalie M. Clark and Rosangela Sozzani
17.Studying Protein-Protein Interactions in planta using Advanced Fluorescence Microscopy
Marc Somssich and Rьdiger Simon
18. Chemiluminescence Based Detection of Peptide Activity and Peptide-Receptor Binding in Plants
Mari Wildhagen, Markus Albert, and Melinka A. Butenko
19. Application of Chemical Genomics to Plant-Bacteria Communication: A High-Throughput System to Identify Novel Molecules Modulating the Induction of Bacterial Virulence Genes by Plant Signals
Elodie Vandelle, Maria Rita Puttilli, Andrea Chini, Giulia Devescovi, Vittorio Venturi, and Annalisa Polverari
Описание: 1. Elicitation Approaches for Withanolide Production in Hairy Root Culture of Withania somnifera (L.) Dunal Ganeshan Sivanandhan, Natesan Selvaraj, Andy Ganapathi, and Markandan Manickavasagan 2. Stimulant Paste Preparation and Bark Streak Tapping Technique for Pine Oleoresin Extraction Thanise Nogueira, Fьller, Jъlio Cйsar de Lima, Fernanco de Costa, Kelly C.S. Rodrigues-Corrкa, and Arthur G. Fett-Neto 3. A Modified Protocol for High Quality RNA Extraction from Oleoresin-Producing Adult Pines Jъlio Cйsar de Lima, Thanise Nogueira Fьller, Fernanda de Costa, Kelly C.S. Rodrigues-Corrкa, and Arthur G. Fett-Neto 4. Collection of Apoplastic Fluids from Arabidopsis thaliana Leaves Svend Roesen Madsen, Hussam Hassan Nour-Eldin, and Barbara Ann Halkier 5. From Plant Extract to a cDNA-Encoding a Glucosyltransferase Candidate: Proteomics and Transcriptomics as Tools to Help Elucidate Saponin Biosynthesis in Centella asiatica Fernando de Costa, Carla J.S. Barber, Darwin W. Reed, and Patrick S. Covello 6. Production of Recombinant Caffeine Synthase from Guarana (Paullinia cupana var. sorbillis) in Escherichia coli Flбvia Camila Schimpl, Eduardo Kiyota, and Paulo Mazzafera 7. Heterologous Expression and Characterization of Mimosinase from Leucaena leucocephala Vishal Singh Negi and Dulal Borthakur 8. Production of Aromatic Plant Terpenoids in Recombinant Baker's Yeast Anita Emmerstorfer and Harald Pichler 9. Purification of a Recombinant Polyhistidine-Tagged Glycosyltransferase Using Immobilized Metal-Affinity Chromatography (IMAC) Fernanda de Costa, Carla J.S. Barber, Pareshkumar T. Pujara, Darwin W. Reed, and Patrick S. Covello 10. A Western Blot Protocol for Detection of Proteins Heterologously Expressed in Xenopus laevis Oocytes Morten Egevang Jrgensen, Hussam Hassan Nour-Eldin, and Barbara Ann Halkier 11. Laser Capture Microdissection: Avoiding Bias in Analysis by Selecting Just What Matters Mбrcia R. de Almeida and Martina V. Strцmvik
12. Analytical and Fluorimetric Methods for the Characterization of the Transmembrane Transport of Specialized Metabolites in Plants Inкs Carqueijeiro, Viviana Martins, Henrique Noronha, Hernвni Gerуs, and Mariana Sottomayor 13. Protoplast Transformation as a Plant-Transferable Transient Expression System Patrнcia Duarte, Diana Ribeiro, Inкs Carqueijeiro, Sara Bettencourt, and Mariana Sottomayor
14. Design, Construction, and Validation of Artificial MicroRNA Vectors Using Agrobacterium-Mediated Transient Expression System Basdeo Bhagwat, Ming Chi, Dianwei Han, Haifeng Tang, Guiliang Tang, and Yu Xiang
15. Knockdown of Polyphenol Oxidase Gene Expression in Potato (Solanum tuberosum L.) with Artificial MicroRNAs Ming Chi, Basdeo Bhagwat, Guiliang Tang, and Yu Xiang
Автор: Hehl Reinhard Название: Plant Synthetic Promoters: Methods and Protocols ISBN: 1493981862 ISBN-13(EAN): 9781493981861 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Practical and cutting-edge, Plant Synthetic Promoters: Methods and Protocols covers all steps required from the identification of cis-regulatory elements, over synthetic promoter design, to the experimental analysis of synthetic promoter function.
Автор: Libo Shan; Ping He Название: Plant Pattern Recognition Receptors ISBN: 1493968580 ISBN-13(EAN): 9781493968589 Издательство: Springer Рейтинг: Цена: 20962.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume covers protocols on techniques ranging from MAMP isolations from diverse microorganisms, PRR identifications from different plant species, MAMP-PRR binding, and a series of signaling responses and events revealed by various biochemical, cellular, genetic and bioinformatic tools.
Автор: Taylor Nicolas L., Millar A. Harvey Название: Isolation of Plant Organelles and Structures: Methods and Protocols ISBN: 1493982281 ISBN-13(EAN): 9781493982288 Издательство: Springer Рейтинг: Цена: 16070.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This book brings together the major techniques used in the isolation or enrichment of individual populations of organelles and other subcellular structures from plants with the goal that, by being able to isolate subcellular structures, the research and understanding of various facets of compartmentalized function in plant cells can be advanced.
Автор: Dandekar Thomas, Naseem Muhammad Название: Auxins and Cytokinins in Plant Biology: Methods and Protocols ISBN: 1493983113 ISBN-13(EAN): 9781493983117 Издательство: Springer Рейтинг: Цена: 13275.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
Preface... Table of Contents... Contributing Authors...
Methodological Advances in Auxin and Cytokinin Biology Andrej Hurnэ and Eva Benkovб
Analytical Determination of Auxins and Cytokinins Petre I. Dobrev, Klбra Hoyerovб, and Jan Petrбsek
Manipulation of Auxin and Cytokinin Balance During the Plasmodiophora brassicae: Arabidopsis thaliana Interaction Jutta Ludwig-Mьller, Susann Auer, Sabine Jьlke, and Sabine Marschollek
Microbial Manipulation of Auxin and Cytokinins in Plants Anwar Hussain, Uhsan Ullah, and Shahida Hasnain
A Standardized Method to Assess Infection Rates of Root-Knot and Cyst Nematodes in Arabidopsis thaliana Mutants with Alterations in Root Development Related to Auxin and Cytokinin Signaling Rocнo Olmo, Ana Clбudia Silva, Fernando E. Dнaz-Manzano, Javier Cabrera, Carmen Fenoll, and Carolina Escobar
Reconstruction of an Immune Dynamic Model to Simulate the Contrasting Role of Auxin and Cytokinin in Plant Immunity Martin Kaltdorf, Dandekar Thomas, and Muhammad Naseem
Interplay between Auxin and Cytokinin and its Impact on Mitogen Activated Protein Kinase (MAPK) Pallavi Singh and Alok Krishna Sinha
Quantification of Cytokinin Levels and Responses in Abiotic Stresses Alfonso Albacete
Assessment of Cytokinin-Induced Immunity through Quantification of Hyaloperonospora arabidopsidis Infection in Arabidopsis thaliana Ruth A. Watson and Cris T. Argueso
Real-Time Genetic Manipulations of the Cytokinin Pathway: A Tool for Laboratory and Field Studies Martin Schдfer and Stefan Meldau
Modulating the Levels of Plant Hormone Cytokinins at the Host-Pathogen Interface Muhammad Naseem, Shabana Shams, and Thomas Roitsch
Analyzing Cytokinin Responses during Plant-Nematode Interactions Florian M.W. Grundler and Shahid Siddique
Examining H2O2 Production in Arabidopsis Leaves Upon Challenge by Cytokinin Shahjahan Shabbir Ahmed, Ihsan Ullah, Shazia Irfan, and Nazeer Ahmed
A Systems Biology Methodology Combining Transcriptome and Interactome Datasets to Assess the Implications of Cytokinin Signaling for Plant Immune Networks Meik Kunz, Thomas Dandekar, and Muhammad Naseem
Monitoring of Crosstalk between Jasmonate and Auxin in the Framework of Plant Stress Responses of Roots Vнctor Carrasco Loba, Marta-Marina Pйrez Alonso, and Stephan Pollmann
High-Throughput Protoplast Trans-Activation (PTA) Screening to Define Transcription Factors in Auxin-Mediated Gene Regulation Nora Whner, Jцrn Herfert, Wolfgang Drцge-Laser, and Christoph Weiste
Автор: Kleine-Vehn Jьrgen, Sauer Michael Название: Plant Hormones: Methods and Protocols ISBN: 1493982109 ISBN-13(EAN): 9781493982103 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
Real Time Analysis of the Apical Hook Development.- Grafting with Arabidopsis thaliana.- Tips and Tricks for Exogenous Application of Synthetic Post-Translationally Modified Peptides to Plants.- Assaying Germination and Seedling Responses of Arabidopsis to Karrikins.- Low-cost Microprocessor-Controlled Rotating Stage for Medium-Throughput Time-Lapse Plant Phenotyping.- Genome-Wide Association Mapping of Root Traits in the Context of Plant Hormone Research.- High throughput scoring of Seed Germination.- Histochemical Staining of β-Glucuronidase and its Spatial Quantification.- Imaging TCSn:: GFP, A Synthetic Cytokinin Reporter, in Arabidopsis thaliana.- Highlighting Gibberellins Accumulation Sites in Arabidopsis Thaliana Root Using Fluorescently Labeled Gibberellins.- In-silico Methods for Cell Annotation, Quantification of Gene Expression and Cell Geometry at Single Cell Resolution using 3DCellAtlas.- Analysing Cell Wall Elasticity after Hormone Treatment: An example using Tobacco BY-2 Cells and Auzin.- FRET-FLIM for Visualizing and Quantifying Protein Interactions in Live Plant Cells.- In vivo Identification of Plant Protein Complexes using IP-MS/MS.- Assaying auxin Receptor Activity using SPR assays with F-Box Proteins and Aux-IAA degrons.- Studying Transcription Factor Binding To Specific Genomic Loci by Chromatin Immunoprecipitation (ChIP).- Hormone Receptor Glycosylation.- Highly Sensitive Salicylic Acid Quantification in Milligram Amounts of Plant Tissue.- High-Resolution Cell-Type Specific Analysis of Cytokinins in Sorted Root Cell Populations of Arabidopsis thaliana.- Hormone Profiling in Plant Tissues.- Use of Xenopus laevis Oocytes to Study Auxin Transport.- Characterizing Auxin Response Circuits in Saccharomyces cerevisiae by Flow Cytometry.
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