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Small Non-Coding Rnas: Methods and Protocols, Rederstorff Mathieu

Rederstorff Mathieu Small Non-Coding Rnas: Methods and Protocols 9781071613856

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Автор: Rederstorff Mathieu
Название: Small Non-Coding Rnas: Methods and Protocols
ISBN: 9781071613856
Издательство: Springer
Классификация:

Генетика (немедицинская)

ISBN-10: 1071613855
Обложка/Формат: Hardcover
Страницы: 270
Вес: 0.63 кг.
Дата издания: 03.05.2021
Язык: English
Размер: 25.91 x 19.56 x 1.78 cm
Ссылка на Издательство: Link
Поставляется из: Германии
Описание: This detailed book aims to provide an overview of novel bioinformatics resources for exploring diverse aspects of RNA biology. This edition focuses on methods dealing with non-coding RNA (miRNAs, circRNAs or lncRNAs), RNA modifications (m6A or RNA editing), single cell RNA-seq and statistical models to handle count data from RNA-seq experiments.

Small Non-Coding RNAs: Methods and Protocols

Автор: Rederstorff Mathieu
Название: Small Non-Coding RNAs: Methods and Protocols
ISBN: 107161388X ISBN-13(EAN): 9781071613887
Издательство: Springer
Рейтинг:
Цена: 16070.00 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание:

PART I INTRODUCTION TO SMALL NON-CODING RNAS

1 Regulatory Non-coding RNAs: An Overview

Camille Virciglio, Yoann Abel, and Mathieu Rederstorff

2 RNA Precipitation

Valйrie Bourguignon-Igel, Yoann Abel, and Mathieu Rederstorff

3 Quantitative and Qualitative Assessment of Small RNA Preparations

Virginie Marchand, Lilia Ayadi, Valйrie Bourguignon-Igel, and Yuri Motorin

4 Non-coding RNA Silencing in Mammalian Cells by Antisense LNA GapmeRs Transfection

Charbel Alfeghaly, Christelle Aigueperse, Sylvain Maenner, and Isabelle Behm-Ansmant

PART II DETECTION OF SMALL NON-CODING RNAS

5 Northern Blot Detection of Tiny RNAs

Jana C. Wiegard, M. Amri C. Schlьter, Olga Y. Burenina, Elena A. Kubareva, Gabriele Klug, Arnold Grьnweller, and Roland K. Hartmann

6 Stem-loop qRT-PCR Based Quantification of miRNAs

Yoann Abel and Mathieu Rederstorff

7 Detection and Labeling of Small Non-coding RNAs by Splinted Ligation

Marlиne Vayssiиres, Gabrielle Bourgeois, Florian Chardon, Anne-Sophie Tillault, and Magali Blaud

8 Fluorescence In Situ Hybridization Of Small Non-coding RNAs

Valentin Vautrot, Gйraud Heckler, Christelle Aigueperse, and Isabelle Behm-Ansmant

PART III FUNCTIONAL ANALYSIS OF SMALL NON-CODING RNAS

9 Using native RIP, UV-CLIP or fCLIP to Address Protein-RNA Interactions In Vivo

Layea Baldini and Stйphane Labialle

10 Purification of RiboNucleoProtein Particles by MS2-MBP Affinity Chromatography

Aymeric Sanchez and Sylvain Maenner

11 Study of genome-wide occupancy of long non-coding RNAs using Chromatin Isolation by RNA Purification (ChIRP)

Charbel Alfeghaly, Isabelle Behm-Ansmant, and Sylvain Maenner

12 Gene Reporter Assays to Study miRNA Function

Yoann Abel and Mathieu Rederstorff

13 CRISPR/Cas9 System to Knock-Down microRNA In Vitro and In Vivo

Bin Yi, Kristina Larter, and Yaguang Xi

PART IV HIGH-THROUGHPUT ANALYSIS OF SMALL NON-CODING RNAS

14 Microarray Analysis of Whole-Transcriptome RNAs including Non-Coding RNAs

Quentin Thuillier and Isabelle Behm-Ansmant

15 Isolation, Extraction, and Deep-Sequencing Analysis Of Extracellular RNAs (exRNAs) from Human Plasma

Virginie Marchand, Adeline Galvanin, and Yuri Motorin

16 Analysis of the RNA and protein complexome by Grad-seq

Jens Hцr and Jцrg Vogel

17 IVC-Seq: A Method for Ultrahigh-throughput Development and Functional Characterization of Small RNAs

Farah Bouhedda, Roger Cubi, Stйphanie Baudrey and Michael Ryckelynck

PART V STRUCTURAL APPROACHES

18 RNA Secondary Structure Study by Chemical Probing Methods using DMS and CMCT

Fatima Alghoul, Gilbert Eriani, and Franck Martin

19 Application of NMR Spectroscopy to Determine the 3D structure of Small Non-coding RNAs

Marie-Eve Chagot, Marc Quinternet, Xavier Manival, and Isabelle Lebars

Plant Circular Rnas: Methods and Protocols

Автор: Vaschetto Luis Marнa
Название: Plant Circular Rnas: Methods and Protocols
ISBN: 1071616447 ISBN-13(EAN): 9781071616444
Издательство: Springer
Цена: 16769.00 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание: This volume presents readers with up-to-date protocols, bioinformatics toolkits, and reference material for understanding circRNAs in plants.

Long Non-Coding Rnas In Plants

Автор: Upadhyay, Santosh Kumar
Название: Long Non-Coding Rnas In Plants
ISBN: 012821452X ISBN-13(EAN): 9780128214527
Издательство: Elsevier Science
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Цена: 26444.00 р.
Наличие на складе: Поставка под заказ.

Описание: Letter Jam is a 2-6 player cooperative word game where players assist each other in composing meaningful words from letters around the table. The trick is holding the letter card so that it`s only visible to other players and not to you.At the start of the game, each player receives a set of face-down letter cards that can be arranged to form an existing word. The setup can be prepared by using a special card scanning app, or by players selecting words for each other. Each player then puts their first card in their stand facing the other players without looking at it, and the game begins.The game is played in turns. Each turn, players simultaneously search other players` letters to see what words they can spell out (telling the others the length of the word they can make up). The player who offers the longest word can then be chosen as the clue giver.The clue giver spells out their clue by putting numbered tokens in front of the other players. Number one goes to the player whose letter comes first in the clue, number two to the second letter etc. They can always use a wild card which can be any letter, but they cannot tell others which letter it represents.Each player with a numbered token (or tokens) in front of them then tries to figure out what their letter is. If they do, they place the card face down before revealing the next letter. At the end of the game, players can then rearrange the cards to try to form an existing word. All players then reveal their cards to see if they were successful or not. The more players who have an existing word in front of them, the bigger their common success.

Regulation of Gene Expression by Small RNAs

Название: Regulation of Gene Expression by Small RNAs
ISBN: 1138111732 ISBN-13(EAN): 9781138111738
Издательство: Taylor&Francis
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Цена: 11482.00 р.
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Описание:

New Findings Revolutionize Concepts of Gene Function

Endogenous small RNAs have been found in various organisms, including humans, mice, flies, worms, fungi, and bacteria. Furthermore, it's been shown that microRNAs acting as cellular rheostats have the ability to modulate gene expression. In higher eukaryotes, microRNAs may regulate as much as 50 percent of gene expression.

Regulation of Gene Expression by Small RNAs brings together the pioneering work of researchers who discuss their work involving a wide variety of small RNA regulatory pathways in organisms ranging from bacteria to humans. In addition to exploring the biogenesis and processing of these regulatory RNAs, they also consider the functional importance of these pathways in host organisms. Assisting current and future researchers, this unique groundbreaking work --

Provides a suite of cutting-edge resources for the study of microRNA ontology and function

Includes a technology guide for those seeking to assay microRNA expression

Explores the mechanisms by which microRNAs regulate gene expression in animal cells, including the regulation of gene expression by RNA-mediated transcriptional gene silencing

Discusses a fast and low-cost approach for reversing genetic influences in mammals

Looks at breakthroughs in the use of microRNA-based therapy for HIV and cancer

This volume captures the essence of the breadth and excitement surrounding the newly discovered regulatory roles of small RNAs. The powerful new approach in the study of gene function described in this text is leading to some remarkable findings that have the potential to revolutionize our understanding of genetic function and the treatment of diseases otherwise considered intractable.

Functional Analysis of Long Non-Coding Rnas: Methods and Protocols

Автор: Cao Haiming
Название: Functional Analysis of Long Non-Coding Rnas: Methods and Protocols
ISBN: 1071611577 ISBN-13(EAN): 9781071611579
Издательство: Springer
Цена: 15372.00 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание: This volume in the Encyclopedia of Sustainability Science and Technology, Second Edition, describes the breadth of science and engineering knowledge critical to advancing sustainable built environments, from architecture and design, mechanical engineering, lighting, and materials to water and energy, public policy, and economics.

Small Non-Coding RNAs

Автор: Mathieu Rederstorff
Название: Small Non-Coding RNAs
ISBN: 1493925466 ISBN-13(EAN): 9781493925469
Издательство: Springer
Рейтинг:
Цена: 19564.00 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание: Small Non-Coding RNAs

Molecular Biology of Long Non-coding RNAs

Автор: Ahmad M. Khalil
Название: Molecular Biology of Long Non-coding RNAs
ISBN: 3030170853 ISBN-13(EAN): 9783030170851
Издательство: Springer
Рейтинг:
Цена: 15372.00 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание: This second edition shows how long non-coding RNAs (lnc)RNAs have emerged as a new paradigm in epigenetic regulation of the genome. Thousands of lncRNAs have been identified and observed in a wide range of organisms. Unlike mRNA, lncRNA have no protein-coding capacity. So, while their function is not entirely clear, they may serve as key organizers of protein complexes that allow for higher order regulatory events. Advances in the field also include better characterization of human long non-coding RNAs, novel insights into their roles in human development and disease, their diverse mechanisms of action and novel technologies to study them.

Non-coding RNAs at the Cross-Road of Cardiometabolic Diseases and Cancer

Автор: Holvoet Paul
Название: Non-coding RNAs at the Cross-Road of Cardiometabolic Diseases and Cancer
ISBN: 3030688461 ISBN-13(EAN): 9783030688462
Издательство: Springer
Рейтинг:
Цена: 19564.00 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание: This book gives insight into the functional role of non-coding RNAs in central pathways contributing to the development of obesity, type 2 diabetes, non-alcoholic fatty liver disease, atherosclerosis, myocardial infarction, cardiomyopathy, and heart failure.

Molecular Biology of Long Non-Coding Rnas

Автор: Khalil Ahmad M.
Название: Molecular Biology of Long Non-Coding Rnas
ISBN: 3030170888 ISBN-13(EAN): 9783030170882
Издательство: Springer
Рейтинг:
Цена: 15372.00 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание: This second edition shows how long non-coding RNAs (lnc)RNAs have emerged as a new paradigm in epigenetic regulation of the genome. Thousands of lncRNAs have been identified and observed in a wide range of organisms. Unlike mRNA, lncRNA have no protein-coding capacity. So, while their function is not entirely clear, they may serve as key organizers of protein complexes that allow for higher order regulatory events. Advances in the field also include better characterization of human long non-coding RNAs, novel insights into their roles in human development and disease, their diverse mechanisms of action and novel technologies to study them.

Non-Coding Rnas at the Cross-Road of Cardiometabolic Diseases and Cancer

Автор: Holvoet Paul
Название: Non-Coding Rnas at the Cross-Road of Cardiometabolic Diseases and Cancer
ISBN: 3030688437 ISBN-13(EAN): 9783030688431
Издательство: Springer
Цена: 19564.00 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание: This book gives insight into the functional role of non-coding RNAs in central pathways contributing to the development of obesity, type 2 diabetes, non-alcoholic fatty liver disease, atherosclerosis, myocardial infarction, cardiomyopathy, and heart failure.

Plant Long Non-Coding RNAs

Автор: Julia A. Chekanova; Hsiao-Lin V. Wang
Название: Plant Long Non-Coding RNAs
ISBN: 1493990446 ISBN-13(EAN): 9781493990443
Издательство: Springer
Рейтинг:
Цена: 25155.00 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание: This volume focuses on various approaches to studying long non-coding RNAs (lncRNAs), including techniques for finding lncRNAs, localization, and observing their functions. The chapters in this book cover how to catalog lncRNAs in various plant species; determining subcellular localization; protein interactions; structures; and RNA modifications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and innovative, Plant Long Non-Coding RNAs: Methods and Protocols is a valuable resource that aids researchers in understanding the functions of lncRNAs in different plant species, and helps them explore currently uncharted facets of plant biology.

Plant Circular RNAs

Автор: Vaschetto
Название: Plant Circular RNAs
ISBN: 1071616471 ISBN-13(EAN): 9781071616475
Издательство: Springer
Рейтинг:
Цена: 16769.00 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание: This volume presents readers with up-to-date protocols, bioinformatics toolkits, and reference material for understanding circRNAs in plants.

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