Ras Activity and Signaling: Methods and Protocols, Rubio Ignacio, Prior Ian
Автор: Rubio Ignacio, Prior Ian Название: Ras Activity and Signaling: Methods and Protocols ISBN: 1071611925 ISBN-13(EAN): 9781071611920 Издательство: Springer Рейтинг: Цена: 18167.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume compiles new experimental approaches and concepts focusing mostly, but not solely, on ways to manipulate and regulate Ras activity and its downstream signaling output.
Автор: Lorenza Trabalzini; Saverio Francesco Retta Название: Ras Signaling ISBN: 162703790X ISBN-13(EAN): 9781627037907 Издательство: Springer Рейтинг: Цена: 23757.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
1. The Ras Superfamily of Small GTPases: The Unlocked Secrets
Luca Goitre, Eliana Trapani, Lorenza Trabalzini, and Saverio Francesco Retta
2. A Novel Method for the Production of Fully-Modified K-Ras 4B
Tanmay S. Chavan, James O. Meyer, Luke Chisholm, Malgorzata Dobosz, and Vadim Gaponenko
3. Metabolic Labeling of Ras with Tritiated Palmitate to Monitor Palmitoylation and Depalmitoylation
Frederick D. Tsai, Joseph P. Wynne, Ian M. Ahearn, and Mark R. Philips
4. Ubiquitination of Rac1 by Inhibitors of Apoptosis (IAPs)
Tripat Kaur Oberoi-Khanuja and Krishnaraj Rajalingam
5. Ras GTPases are Both Regulators and Effectors of Redox Agents
Elisa Ferro, Luca Goitre, Eva Baldini, Saverio Francesco Retta, and Lorenza Trabalzini
6. Biophysical and Proteomic Characterization Strategies for Cysteine Modifications in Ras GTPases
G. Aaron Hobbs, Harsha P. Gunawardena, and Sharon L. Campbell
7. Use of the Yeast Two-Hybrid Technology to Isolate Molecular Interactions of Ras GTPases
Elisa Ferro, Eva Baldini, and Lorenza Trabalzini
8. Screening for MAPK Modulators Using an In-Cell Western Assay
Simon Schnaiter, Beatrix Fьrst, Johannes Neu, Frigyes Wбczek, Lбszlу Őrfi, Gyцrgy Kйri, Lukas A. Huber, and Winfried Wunderlich
9. Behavioral Methods for the Study of the Ras-ERK Pathway in Memory Formation and Consolidation: Passive Avoidance and Novel Object Recognition Tests
Raffaele d'Isa, Riccardo Brambilla, and Stefania Fasano
10. Functional Phosphoproteomics of Oncogenic KRAS Signaling
Putty-Reddy Sudhir and Jeou-Yuan Chen
11. Pull-Down Assay for Analysis of Integrin-Mediated Activation of Rap Proteins in Adherent Platelets
Gianni Francesco Guidetti and Mauro Torti
12. Combined Pulldown and Time-Lapse Microscopy Studies for Determining the Role of Rap1 in the Crosstalk between Integrins and Cadherins
Luca Goitre, Alessandro Morina, and Saverio Francesco Retta
13. Fluorescence Microscopy Study of Rap1 Subcellular Localization
Luca Goitre, Valentina Cutano, and Saverio Francesco Retta
14. An In Vitro System to Evaluate the Scaffold Function of the RalA Effector Protein RalBP1
David F. Kashatus
15. Analysis of the Rit Subfamily GTPase-Mediated Signaling and Neuronal Differentiation and Survival
Geng-Xian Shi and Catherine N. Kaminski
16. Immunofluorescence Methods in Studies of the GTPase RAN and Its Effectors in Interphase and in Mitotic Cells
Giulia Guarguaglini, Valeria de Turris, and Patrizia Lavia
17. High Resolution Scanning Electron Microscopy for the Imaging of Nuclear Pore Complexes and Ran-Mediated Transport
Lihi Shaulov, Boris Fichtman, and Amnon Harel
18. Effector Recruitment Method to Study Spatially Regulated Activation of Ras and Rho GTPases
Lauren P. Huff, Molly J. DeCristo, and Adrienne D. Cox
19. Real-Time Visualization and Quantification of Native Ras Activation in Single Living Cells
Christoph Biskup and Ignacio Rubio
20. Nanoclustering and Heterogeneous Membrane Diffusion of Ras Studied by FRAP and RICS Analysis
Camilo Guzmбn, Maja Solman, and Daniel Abankwa
21. Analyzing the Roles of Rho GTPases in Cancer Cell Migration with a Live Cell Imaging 3D-Morphology-Based Assay
Audrey Colomba and Anne J. Ridley
22. Analysis of Rho-GTPase-Induced Localization of Nanoscale Adhesions Using Fluorescence Nanoscopy
Annica K.B. Gad, Daniel Rцnnlund, Jerker Widengren, and Pontus Aspenstrцm
23. Yeast as a Model for Ras Signaling
Renata Tisi, Fiorella Belotti, and Enzo Martegani
24.
Автор: Lorenza Trabalzini; Saverio Francesco Retta Название: Ras Signaling ISBN: 1493960547 ISBN-13(EAN): 9781493960545 Издательство: Springer Рейтинг: Цена: 16769.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
1. The Ras Superfamily of Small GTPases: The Unlocked Secrets
Luca Goitre, Eliana Trapani, Lorenza Trabalzini, and Saverio Francesco Retta
2. A Novel Method for the Production of Fully-Modified K-Ras 4B
Tanmay S. Chavan, James O. Meyer, Luke Chisholm, Malgorzata Dobosz, and Vadim Gaponenko
3. Metabolic Labeling of Ras with Tritiated Palmitate to Monitor Palmitoylation and Depalmitoylation
Frederick D. Tsai, Joseph P. Wynne, Ian M. Ahearn, and Mark R. Philips
4. Ubiquitination of Rac1 by Inhibitors of Apoptosis (IAPs)
Tripat Kaur Oberoi-Khanuja and Krishnaraj Rajalingam
5. Ras GTPases are Both Regulators and Effectors of Redox Agents
Elisa Ferro, Luca Goitre, Eva Baldini, Saverio Francesco Retta, and Lorenza Trabalzini
6. Biophysical and Proteomic Characterization Strategies for Cysteine Modifications in Ras GTPases
G. Aaron Hobbs, Harsha P. Gunawardena, and Sharon L. Campbell
7. Use of the Yeast Two-Hybrid Technology to Isolate Molecular Interactions of Ras GTPases
Elisa Ferro, Eva Baldini, and Lorenza Trabalzini
8. Screening for MAPK Modulators Using an In-Cell Western Assay
Simon Schnaiter, Beatrix Fьrst, Johannes Neu, Frigyes Wбczek, Lбszlу Őrfi, Gyцrgy Kйri, Lukas A. Huber, and Winfried Wunderlich
9. Behavioral Methods for the Study of the Ras-ERK Pathway in Memory Formation and Consolidation: Passive Avoidance and Novel Object Recognition Tests
Raffaele d'Isa, Riccardo Brambilla, and Stefania Fasano
10. Functional Phosphoproteomics of Oncogenic KRAS Signaling
Putty-Reddy Sudhir and Jeou-Yuan Chen
11. Pull-Down Assay for Analysis of Integrin-Mediated Activation of Rap Proteins in Adherent Platelets
Gianni Francesco Guidetti and Mauro Torti
12. Combined Pulldown and Time-Lapse Microscopy Studies for Determining the Role of Rap1 in the Crosstalk between Integrins and Cadherins
Luca Goitre, Alessandro Morina, and Saverio Francesco Retta
13. Fluorescence Microscopy Study of Rap1 Subcellular Localization
Luca Goitre, Valentina Cutano, and Saverio Francesco Retta
14. An In Vitro System to Evaluate the Scaffold Function of the RalA Effector Protein RalBP1
David F. Kashatus
15. Analysis of the Rit Subfamily GTPase-Mediated Signaling and Neuronal Differentiation and Survival
Geng-Xian Shi and Catherine N. Kaminski
16. Immunofluorescence Methods in Studies of the GTPase RAN and Its Effectors in Interphase and in Mitotic Cells
Giulia Guarguaglini, Valeria de Turris, and Patrizia Lavia
17. High Resolution Scanning Electron Microscopy for the Imaging of Nuclear Pore Complexes and Ran-Mediated Transport
Lihi Shaulov, Boris Fichtman, and Amnon Harel
18. Effector Recruitment Method to Study Spatially Regulated Activation of Ras and Rho GTPases
Lauren P. Huff, Molly J. DeCristo, and Adrienne D. Cox
19. Real-Time Visualization and Quantification of Native Ras Activation in Single Living Cells
Christoph Biskup and Ignacio Rubio
20. Nanoclustering and Heterogeneous Membrane Diffusion of Ras Studied by FRAP and RICS Analysis
Camilo Guzmбn, Maja Solman, and Daniel Abankwa
21. Analyzing the Roles of Rho GTPases in Cancer Cell Migration with a Live Cell Imaging 3D-Morphology-Based Assay
Audrey Colomba and Anne J. Ridley
22. Analysis of Rho-GTPase-Induced Localization of Nanoscale Adhesions Using Fluorescence Nanoscopy
Annica K.B. Gad, Daniel Rцnnlund, Jerker Widengren, and Pontus Aspenstrцm
23. Yeast as a Model for Ras Signaling
Renata Tisi, Fiorella Belotti, and Enzo Martegani
24.
Автор: Jonathan P. Rast; James W.D. Booth Название: Immune Receptors ISBN: 1493957988 ISBN-13(EAN): 9781493957989 Издательство: Springer Рейтинг: Цена: 20263.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This book presents technical descriptions and protocols for a variety of experimental approaches to the characterization of immune receptors and the cell biology that mediates their functions. Written in the successful Methods in Molecular Biology (TM) format.
Автор: Alfred Wittinghofer Название: Ras Superfamily Small G Proteins: Biology and Mechanisms 1 ISBN: 370911988X ISBN-13(EAN): 9783709119884 Издательство: Springer Рейтинг: Цена: 19589.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: The focus of this first volume is on the Rho and Ras subfamily of small G proteins.Renowned scientists provide insights into the biochemistry of the classical and non-classical small G-protein family members, their spatio-temporal regulation, their effectors and their roles in health and disease.
Автор: Alfred Wittinghofer Название: Ras Superfamily Small G Proteins: Biology and Mechanisms 1 ISBN: 3709118050 ISBN-13(EAN): 9783709118054 Издательство: Springer Рейтинг: Цена: 22359.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This first of two volumes provides a general overview of the genetics, structure, mechanism and regulation of the Ras superfamily proteins and describes in detail the signaling pathways and processes regulated by specific members of this family. The focus of this first volume is on the Rho and Ras subfamily of small G proteins. Renowned scientists provide insights into the biochemistry of the classical and non-classical small G-protein family members, their spatio-temporal regulation, their effectors and their roles in health and disease. Together with Volume 2, this book provides a comprehensive and state-of-the-art work on small G-proteins (GTPases). It is intended for graduates and professors in biochemistry and cell biology already working on small G-proteins (small GTPases), but also offers an extremely valuable resource for those readers who are new to the field.
Автор: Mark P. Running Название: G Protein-Coupled Receptor Signaling in Plants ISBN: 1493962167 ISBN-13(EAN): 9781493962167 Издательство: Springer Рейтинг: Цена: 14673.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This Methods in Molecular Biology book describes techniques used in the study of small GTPases and related proteins. Includes a chapter on bioinformatics approaches, plus reproducible protocols, materials lists, practical tips and troubleshooting advice.
Автор: Jamila I. Horabin Название: Hedgehog Signaling Protocols ISBN: 161737721X ISBN-13(EAN): 9781617377211 Издательство: Springer Рейтинг: Цена: 21661.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Intended for Molecular Biologists, Biochemists, and Geneticists interested in manipulating and analyzing the Hedgehog Signaling Pathway, this volume offers various detailed chapters on experimental description, including anecdotal pointers and notes.
Автор: Mario Tiberi Название: G Protein-Coupled Receptor Signaling ISBN: 1493991205 ISBN-13(EAN): 9781493991204 Издательство: Springer Рейтинг: Цена: 25155.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This detailed volume assembles comprehensive protocols to assist with the study of structural, molecular, cell biological, and in vivo facets of GPCRs, and to enable the development of experimental tools for screening novel GPCR drugs. Sections explore the tweaking of ligands, bioluminescence and FRET approaches, specific GPCR signaling properties, as well as visualization of subcellular compartmentalization. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, G Protein-Coupled Receptor Signaling: Methods and Protocols serves as an ideal reference for life scientists working in a variety of research fields including molecular pharmacology, cell and developmental biology, brain behavior and physiology, drug development and screening.
Chapter 4 is available open access under a CC BY 4.0 license via link.springer.com.
Название: Semaphorin signaling ISBN: 1493982036 ISBN-13(EAN): 9781493982035 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
1. Semaphorins and their Signaling Mechanisms
Laura T. Alto and Jonathan R. Terman
Part I: Biochemical Approaches
2. Isolating Fc-Tagged SEMA4D Recombinant Protein from 293FT Cells
Joanne Soong and Glynis A. Scott
3. Expression and Purification of Class 7 Semaphorin and its Plexinc1 Receptor Using Baculovirus-Mediated Mammalian Cell Gene Transduction
Xiaoyan Chen, Po-Han Chen, and Xiaolin He
4. Immunoaffinity Purification of the Glycosylated Extracellular Fragment of Mouse Plexin A2 Produced in a Mammalian Expression System
Terukazu Nogi, Emiko Mihara, Norihisa Yasui, and Junichi Takagi
5. Plate-Based Assay for Measuring Direct Semaphorin-Neuropilin Interactions
Matthew W. Parker and Craig W. Vander Kooi
6. Characterizing Plexin Gtpase Interactions Using Gel Filtration, Surface Plasmon Resonance Spectrometry and Isothermal Titration Calorimetry
Jeannine Muller-Greven, Soonjeung Kim, Prasanta K. Hota, Yufeng Tong, Susmita Borthakur, and Matthias Buck
7. In Vitro Assay for the Rap Gtpase Activating Protein Activity of the Purified Cytoplasmic Domain of Plexin
Heath G. Pascoe, Yuxiao Wang, and Xuewu Zhang
8. Characterizing F-Actin Disassembly Induced by the Semaphorin Signaling Component Mical
Jimok Yoon, Ruei-Jiun Hung, and Jonathan R. Terman
9. Characterizing Erbb-2-Mediated Tyrosine Phosphorylation and Activation of Plexins
Thomas Worzfeld and Jakub M. Swiercz
10. Characterizing PKA-Mediated Phosphorylation of Plexin Using Purified Proteins
Taehong Yang and Jonathan R. Terman
Part II: In Vitro Approaches
11. Using Heterologous Cos-7 Cells to Identify Semaphorin Signaling Components Atsuko Sakurai, Colleen L. Doзi, and J. Silvio Gutkind
12. Analysis of Semaphorin-Induced Growth Cone Collapse and Axon Growth Inhibition
Lionel Meyer, Andrius Kaselis, Saulius Satkauskas, and Dominique Bagnard
13. Using Rotary Shadow Electron Microscopy to Characterize Semaphorin-Mediated Growth Cone Collapse
Paul C. Bridgman
14. An Electrical Impedance-Based Method for Quantitative Real-Time Analysis of Semaphorin-Elicited Endothelial Cell Collapse
Chiara Camillo, Noemi Gioelli, Federico Bussolin, and Guido Serini
15. Regulation of Cortical Dendrite Morphology and Spine Organization by Secreted Semaphorins - A Primary Culture Approach
Sheng-Shiang Peng and Tracy S. Tran
16. Characterizing Semaphorin Signaling Using Isolated Neurons in Culture
Sophie Chauvet, Erik Mire, and Fanny Mann
17. Performing Axon Orientation Assays With Secreted Semaphorins And Other Guidance Cues
Cйline Delloye-Bourgeois, Frйdйric Moret And Valйrie Castellani
18. Assays to Examine Transmembrane Semaphorin Function In Vitro
Rachel Q. Liu, Kristen Browne, and Timothy P. O'Connor
19. Micro-CALI to Study Localized Roles of the Semaphorin Signaling Component CRMP in Axon Growth
Yoshio Goshima and Kohtaro Takei
20. Visualizing and Characterizing Semaphorin Endocytic Events Using Quantum Dot Conjugated Proteins
Ioana Carcea and Deanna L. Benson
21. Visualization of Clathrin-Mediated Endocytosis during Semaphorin-Guided Axonal Growth
Rurika Itofusa, Takuro Tojima, and Hiroyuki Kamiguchi
22. Tracking Differential Endocytosis and Trafficking of Semaphorin Receptor Complexes in Responding Nerve Growth Cones
Puneet Dang and Andrew J. W
Автор: Terman Название: Semaphorin Signaling ISBN: 1493964461 ISBN-13(EAN): 9781493964468 Издательство: Springer Рейтинг: Цена: 25155.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume arranged into three sections describes biochemical, in vitro, and in vivo protocols on Semaphorins. Chapters focus on approaches that would allow the novice to study Semaphorins and employ robust assays to characterize mechanisms of action. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Semaphorin Signaling: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Автор: Mark P. Running Название: G Protein-Coupled Receptor Signaling in Plants ISBN: 1627035311 ISBN-13(EAN): 9781627035316 Издательство: Springer Рейтинг: Цена: 14673.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This Methods in Molecular Biology book describes techniques used in the study of small GTPases and related proteins. Includes a chapter on bioinformatics approaches, plus reproducible protocols, materials lists, practical tips and troubleshooting advice.
ООО "Логосфера " Тел:+7(495) 980-12-10 www.logobook.ru
