Описание: Part I: Bioinformatics
1. Predicting RNA Structure with Vfold
Chenhan Zhao, Xiaojun Xu, and Shi-Jie Chen
2. RNA Function Prediction
Yongsheng Li, Juan Xu, Tingting Shao, Yunpeng Zhang, Hong Chen, and Xia Li
3. Computational Prediction of Novel miRNAs from Genome-Wide Data
Georgina Stegmayer, Cristian Yones, Laura Kamenetzky, Natalia Macchiaroli, and Diego H. Milone
4. Protein Structure Modeling with MODELLER
Benjamin Webb and Andrej Sali
5. Protein Function Prediction
Leonardo Magalhгes Cruz, Sheyla Trefflich, Vinнcius Almir Weiss, and Mauro Antфnio Alves Castro
Part II: DNA Analysis
6. Capturing Three-Dimensional Genome Organization in Individual Cells by Single-Cell Hi-C
Takashi Nagano, Steven W. Wingett, and Peter Fraser
7. Genome-Wide Cell Type-Specific Mapping of In Vivo Chromatin Protein Binding Using an FLP-Inducible DamID System in Drosophila
Alexey V. Pindyurin
8. DNA Methylation Profiling Using Long-Read Single-Molecule Real-Time Bisulfite Sequencing (SMRT-BS)
Yao Yang and Stuart A. Scott
9. Copy Number Variation Analysis by Droplet Digital PCR
Suvi K. Hдrmдlд, Robert Butcher, and Chrissy H. Roberts
10. MicroScale Thermophoresis: A Rapid and Precise Method to Quantify Protein-Nucleic Acid Interactions in Solution
Adrian Michael Mьller, Dennis Breitsprecher, Stefan Duhr, Philipp Baaske, Thomas Schubert, and Gernot Lдngst
11. Establishment of the CRISPR/Cas9 System for Targeted Gene Disruption and Gene Tagging
Eric Ehrke-Schulz, Maren Schiwon, Claudia Hagedorn, and Anja Ehrhardt
Part III: RNA Analysis
12. Holistic and Affordable Analyses of MicroRNA Expression Profiles Using Tagged cDNA Libraries and a Multiplex Sequencing Strategy
Patrick P. Weil, Yan Jaszczyszyn, Anne Baroin-Tourancheau, Jan Postberg, and Laurence Amar
13. MicroRNA Expression Analysis Using Small RNA Sequencing Discovery and RT-qPCR-Based Validation
Alan Van Goethem, Pieter Mestdagh, Tom Van Maerken, and Jo Vandesompele
14. Using Firefly(R) Particle Technology for Multiplex MicroRNA Profiling Without RNA Purification
Michael R. Tackett and Izzudin Diwan
15. Multiplex Real-Time PCR Using Encoded Microparticles for MicroRNA Profiling
Seungwon Jung and Sang Kyung Kim
16. Optimized Whole Transcriptome Profiling of Motor Axons
Lena Saal, Michael Briese, and Michael Sendtner
Part IV: Protein Analysis
17. 2D-DIGE in Proteomics
Matias Pasquali, Tommaso Serchi, Sebastien Planchon, and Jenny Renaut
18. STAGE-Diging in Proteomics
Paolo Soffientini and Angela Bachi
19. Protein Arrays I: Antibody Arrays
Yulin Yuan, Zuan-Tao Lin, Hongting Wang, Xia Hong, Mikala Heon, and Tianfu Wu
20. Protein Arrays II: Antigen Arrays
Yulin Yuan, Hongting Wang, Zuan-Tao Lin, Xia Hong, Mikala Heon, and Tianfu Wu
21. Protein Arrays III: Reverse-Phase Protein Arrays
Yulin Yuan, Xia Hong, Zuan-Tao Lin, Hongting Wang, Mikala Heon, and Tianfu Wu
22. Isolation of Exosomes for the Purpose of Protei
