Electrophoresis in Practice - A Guide to Methods and Applications of DNA and Protein Separations 5e, Westermeier
Автор: L.A. Osterman Название: Methods of Protein and Nucleic Acid Research ISBN: 3642874878 ISBN-13(EAN): 9783642874871 Издательство: Springer Рейтинг: Цена: 16979.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This method enables separation of macromolecules according to characteristic features such as size (or molecular weight), shape, secondary structure and electric charge and these parameters can in- fluence electrophoretic properties either separately or jointly.
Автор: Biji T. Kurien; R. Hal Scofield Название: Protein Electrophoresis ISBN: 1493962418 ISBN-13(EAN): 9781493962419 Издательство: Springer Рейтинг: Цена: 18167.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Proteins are the functional units of the cellular machinery and they provide significant information regarding the molecular basis of health and disease. Therefore, techniques to separate and isolate the various proteins are critical to studying and understanding their functional characteristics. One of the widely used techniques for this purpose is electrophoresis. In Protein Electrophoresis: Methods and Protocols, contributions from experts in the field have been collected in order to provide practical guidelines to this complex study. Each chapter outlines a specific electrophoretic variant in detail so that laboratory scientists may perform a technique new to their lab without difficulty. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Protein Electrophoresis: Methods and Protocols seeks to serve laboratory scientists with well-honed, detailed methodologies in an effort to further our knowledge of this essential field.
Автор: Terry M. Phillips; Heather Kalish Название: Clinical Applications of Capillary Electrophoresis ISBN: 1493962388 ISBN-13(EAN): 9781493962389 Издательство: Springer Рейтинг: Цена: 20263.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This Methods in Molecular Biology (TM) book is an in-depth manual of Capillary Electrophoresis applications in important areas of clinical science, including clinical chemistry, hematology, disease-associated biomarker discovery, immunology and genetic analysis.
Автор: Rainer Cramer; Reiner Westermeier Название: Difference Gel Electrophoresis (DIGE) ISBN: 1493962051 ISBN-13(EAN): 9781493962051 Издательство: Springer Рейтинг: Цена: 20263.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This book introduces DIGE and its advantages in quantitative protein analysis, providing detailed protocols and important notes on the practical aspects of DIGE with both generic and specific applications in the various areas of Quantitative Proteomics.
Автор: Tran Название: Capillary Electrophoresis of Proteins and Peptides ISBN: 1493940120 ISBN-13(EAN): 9781493940127 Издательство: Springer Рейтинг: Цена: 19564.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This book provides a comprehensive survey of recent developments and applications of high performance capillary electrophoresis in the field of protein and peptide analysis with a distinct focus on the analysis of intact proteins. With practical detail, the contents cover different modes of capillary electrophoresis (CE) useful for protein and peptide analysis, CZE, CIEF, ACE, CGE, and different types of application such as the quality control of therapeutic proteins and monoclonal antibodies, clinical analyses of chemokines in tissues, qualitative and quantitative analysis of vaccine proteins, and determination of binding constants in complexes involving peptides or proteins. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and exhaustive, Capillary Electrophoresis of Proteins and Peptides: Methods and Protocols serves both beginners and experts with a collection of the current and most active topics in this vital field of study.
Автор: Schmitt-Kopplin Название: Capillary Electrophoresis ISBN: 1493964011 ISBN-13(EAN): 9781493964017 Издательство: Springer Рейтинг: Цена: 22359.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This new edition presents principle methods in capillary electrophoresis (CE) separation involving CZE, MEKC, MECC, NACE, and corresponding hyphenated techniques to organic mass spectrometry and ICP-MS. Recent developments in the techniques of single cell analysis, as well as derivation, enantioseparation or the use of ionic liquids, and the use of CZE for the separation of living cells are also highlighted. This book discusses various application methods for the analysis of small ions, organic acids, amino acids, and (poly)saccharides to peptides that are shown with pollutants and biomarkers in food and health. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting edge and thorough, Capillary Electrophoresis: Methods and Protocols, Second Edition covers a wide field of interests and will be especially great for beginners and students because of its combined focus on mini-reviews and application notes that will help them quickly get an overview of the field.
Автор: Xia Название: Capillary Electrophoresis-Mass Spectrometry ISBN: 3319462385 ISBN-13(EAN): 9783319462387 Издательство: Springer Рейтинг: Цена: 16769.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This book covers the latest developments in capillary electrophoresis-mass spectrometry for the analysis of therapeutic proteins. The application of capillary electrophoresis-mass spectrometry (CE-MS) coupling technology in the analysis of recombinant therapeutic proteins is detailed thoroughly. Specific topics include recent developments in coupling capillary electrophoresis with mass spectrometry for the quality control of monoclonal antibody therapeutics, top-down analysis of monoclonal antibody using the CE-MS platform, and detection of host cell protein impurities. Comprehensive characterization of antibody-drug conjugates (ADCs) by coupling capillary electrophoresis with mass spectrometry is also covered. This is an ideal book for scientists in the life science and biopharmaceutical industry who are working on characterizing the PTMs of monoclonal antibodies, as well as graduate students and researchers in the separation science and biological mass spectrometry fields.
Автор: Gunter M. Rothe Название: Electrophoresis of Enzymes ISBN: 3642790712 ISBN-13(EAN): 9783642790713 Издательство: Springer Рейтинг: Цена: 18284.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Автор: Kay Ohlendieck Название: Difference Gel Electrophoresis ISBN: 1493972677 ISBN-13(EAN): 9781493972678 Издательство: Springer Рейтинг: Цена: 20962.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Chapters in Difference Gel Electrophoresis: Methods and Protocols detail methods on principles of differential protein labeling, two-dimensional gel electrophoresis, techniques on optimized proteomic workflows, advanced mass spectrometry for protein identification, application of basic biological research, and applied biomarker discovery.
Автор: Ann Van Schepdael Название: Microchip Capillary Electrophoresis Protocols ISBN: 1493923528 ISBN-13(EAN): 9781493923526 Издательство: Springer Рейтинг: Цена: 19564.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
Part I. Overview
1. An Overview of the use of Microchips in Electrophoretic Separation Techniques: Fabrication, Separation Modes, Sample Preparation Opportunities, and On-Chip Detection
Stijn Hendrickx, Wim De Malsche, and Deirdre Cabooter
Part II. Microchip Capillary Electrophoresis Of Small Molecules
2. Microchip Electrophoresis for Fast and Interference-Free Determination of Trace Amounts of Glyphosate and Glufosinate Residues in Agricultural Products
Xuan Wei And Qiaosheng Pu
3. Microchip Capillary Electrophoresis Of Nitrite and Nitrate in Cerebrospinal Fluid
Mariбn Masбr, Rуbert Bodor, and Peter Troska
4. Analysis Of Thiols By Microchip Capillary Electrophoresis for In Situ Planetary Investigations
Maria F. Mora, Amanda M. Stockton, and Peter A. Willis
5. Determination of Ofloxacin in Ofloxacin Ear Drops by Microfluidic Chip with Contactless Conductivity Detection
Bing Chen and Kaicheng Li
Part III. Microchip Capillary Electrophoresis Of Nucleic Acids, Proteins, Peptides, Pathogens
6. Microchip Capillary Electrophoresis: Quantum Dots and Paramagnetic Particles for Bacteria Immunoseparation
Sona Krizkova, Hoai Viet Nguyen, Maja Stanisavljevic, Pavel Kopel, Marketa Vaculovicova, Vojtech Adam, and Rene Kizek
7. Fast High-Throughput Screening Of H1N1 Virus by Parallel Detection with Multi-Channel Microchip Electrophoresis
Peng Zhang, He Nan, Seungah Lee, and Seong Ho Kang
8. Sex Identification of Ancient DNA Samples using a Microfluidic Device
Kirsty J Shaw, Keri A. Brown, Terence A. Brown, and Stephen J. Haswell
9. Fast and Continuous-Flow Detection and Separation Of DNA-Complexes and DNA in Nanofluidic Chip Format
Martina Viefhues, Jan Regtmeier, and Dario Anselmetti
10. Multi-Dimension Microchip-Capillary Electrophoresis Device for Determination of Functional Proteins in Infant Milk Formula
Ruige Wu, Zhiping Wang, and Ying Sing Fung
11. Fast Assembly of Anti-Voltage Photonic Crystals in Microfluidic Channels for Ultrafast Separation of Amino Acids and Peptides
Yi Chen, Tao Liao and Can Hu
Part IV. Nonaqueous Applications
12. Rapid Determination of Catecholamines in Urine Samples by Nonaqueous Microchip Electrophoresis with LIF Detection
Hongmei Hu and Yuanming Guo
Part V. Capillary Electrochromatography On Microchips
13. Carbon Nanotube Based Separation Columns for Microchip Electrochromatography
K. B. Mogensen, B. Delacourt, and J. P. Kutter
14. Electrochromatography on Acrylate-Based Monolith in Cyclic Olefin Copolymer Microchip: an Attractive Technology
Y. Ladner, G. Cretier, and K. Faure
Part VI. Sample Preparation Approaches
15. On-Chip Electromembrane Extraction for Monitoring Drug Metabolism in Real Time by Electrospray Ionization Mass Spectrometry
Nickolaj J. Petersen, Henrik Jensen, and Stig Pedersen-Bjergaard
16. Sample Preparation for N-Glycosylation Analysis of Therapeutic Monoclonal Antibodies By Electrophoresis
Бkos Szekrйnyes, Jan Partyka, Csaba Varadi, Jana Krenkova, Frantisek Foret, and Andrбs Guttman
Автор: Heinz Engelhardt; G. Gutnikov; Wolfgang Beck; Thom Название: Capillary Electrophoresis ISBN: 3642858562 ISBN-13(EAN): 9783642858567 Издательство: Springer Рейтинг: Цена: 13060.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Автор: Nicola Volpi; Francesca Maccari Название: Capillary Electrophoresis of Biomolecules ISBN: 1493962485 ISBN-13(EAN): 9781493962488 Издательство: Springer Рейтинг: Цена: 20263.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This book details key techniques used to investigate Capillary electrophoresis (CE). It focuses on simple and complex carbohydrates (polysaccharides), aminoacids, peptides and proteins, enzymes, and nucleic acids.
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