Light microscopy, Yolanda Markaki

Автор: Michler G.H.
Название: Electron Microscopy of Polymers
ISBN: 3540363505 ISBN-13(EAN): 9783540363507
Издательство: Springer
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Описание: There are many books on electron microscopy, however, the study of polymers by EM needs special techniques, precautions and preparation methods, including ultramicrotomy. General characteristics of the different techniques of EM, including scanning force microscopy (AFM), are given. Application of these techniques to study morphology and also properties, particularly micromechanical properties, is described in detail. Examples from all classes of polymers are presented.

Автор: Hibbs Alan R.
Название: Confocal Microscopy for Biologists
ISBN: 0306484684 ISBN-13(EAN): 9780306484681
Издательство: Springer
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Описание: There has been a great upsurge in interest in light microscopy in recent years due to the advent of a number of significant advances in microscopy, one of the most important of which is confocal microscopy.

Автор: Glauert Audrey M., Lewis Peter R.
Название: Biological Specimen Preparation for Transmission Electron Microscopy
ISBN: 0691600155 ISBN-13(EAN): 9780691600154
Издательство: Wiley
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Описание:

This book contains all the necessary information and advice for anyone wishing to obtain electron micrographs showing the most accurate ultrastructural detail "in thin sections" of any type of biological specimen.

The guidelines for the choice of preparative methods are based on an extensive survey of current laboratory practice. For the first time, in a textbook of this kind, the molecular events occurring during fixation and embedding are analysed in detail. The reasons for choosing particular specimen preparation methods are explained and guidance is given on how to modify established techniques to suit individual requirements.

All the practical methods advocated are clearly described, with accompanying tables and the results obtainable are illustrated with many electron micrographs.

Portland Press Series: "Practical Methods in Electron Microscopy," Volume 17, Audrey M. Glauert, Editor

Originally published in 1999.

The Princeton Legacy Library uses the latest print-on-demand technology to again make available previously out-of-print books from the distinguished backlist of Princeton University Press. These paperback editions preserve the original texts of these important books while presenting them in durable paperback editions. The goal of the Princeton Legacy Library is to vastly increase access to the rich scholarly heritage found in the thousands of books published by Princeton University Press since its founding in 1905.

Автор: Engelborghs
Название: Fluorescence Spectroscopy and Microscopy
ISBN: 1627036482 ISBN-13(EAN): 9781627036481
Издательство: Springer
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Цена: 30745.00 р.
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Описание:

Part I: Steady-State Fluorescence Spectroscopy (SSFS)

1. How to Collect National Institute of Standards and Technology (NIST) Traceable Fluorescence Excitation and Emission Spectra

Adam Matthew Gilmore

2. Steady State Fluorescence Polarization/Anisotropy for the Study of Protein Interactions

Nicholas G. James and David M. Jameson

3. Quantitative Fluorescence Spectral Analysis of Protein Denaturation

Ivo H.M. van Stokkum and Sergey P. Laptenok

4. High-Pressure Fluorescence Applications

Mariano Dellarole and Catherine A. Royer

Part II: Time-Resolved Fluorescence Spectroscopy (TRFS)

5. Frequency Domain Fluorometry: Theory and Application

Carissa M. Vetromile and David M. Jameson

6. Polar Plot Representation of Time-Resolved Fluorescence

John Paul Eichorts, Kai wen Teng, and Robert M. Clegg

7. Ensemble and Single Molecule Detected Time Resolved FRET Methods in Studies of Protein Conformations and Dynamics

Tomer Orevi, Eitan Lerner, Gil Rahamim, Dan Amir, and Elisha Haas

8. MD + QM Correlations with Tryptophan Fluorescence Spectral Shifts and Lifetimes

Patrik R. Callis and Jose R. Tusell

9. Analysis of Time-Dependent Red Shifts in Fluorescence Emission from Tryptophan Residues in Proteins

Dmitri Toptygin

10. Global Analysis of Time-Resolved Fluorescence Data

Anatoli V. Digris, Eugene G. Novikov, Victor V. Skakun, and Vladimir V. Apanasovich

11. Nanometrology

David J.S. Birch and Philip Yip

12. Upconversion Spectrophotofluorometry

Arianna Biesso, Jianhua Xu, and Jay R. Knutson

13. Subpicosecond Kerr-Gate Spectrofluorometry

Sergey P. Laptenok, Patrick Nьrnberger, Andras Lukacs, and Marten H. Vos

14. Photo-Induced Electron Transfer Modeling to Simulate Flavoprotein Fluorescence Decay

Nadtanet Nunthaboot, Kiattisak Lugsanangarm, Arthit Nueangaudom, Somsak Pianwanit, Sirirat Kokpol, and Fumio Tanaka

Part III: Fluorescent Probe Development (FPD)

15. Biosynthetic Incorporation of Trp Analogs in Proteins

Jaap Broos

16. Optimization of Fluorescent Proteins

Daphne S. Bindels, Joachim Goedhart, Mark A. Hink, Laura van Weeren, Linda Joosen, and Theodorus W.J. Gadella, Jr.

17. Monitoring Membrane Properties and Apoptosis Using Membrane Probes of the 3-Hydroxyflavone Family

Zeinab Darwich, Andrey S. Klymchenko, and Yves Mйly

Part IV: Fluorescence Microscopy: Fluorescence Recovery After Photobleaching (FRAP)

18. Rectangle FRAP for Measuring Diffusion with a Laser Scanning Microscope

Ranhua Xiong, Hendrik Deschout, Jo Demeester, Stefaan C. De Smedt, and Kevin Braeckmans

Part V: Fluorescence Microscopy: Fцrster Resonance Energy Transfer Imaging (FRETim)

19. A Quantitative Protocol for Intensity-Based Live Cell FRET Imaging

Clemens F. Kaminski, Eric. J. Rees, and Gabriele S. Kaminski Schierle

Part VI: Fluorescence Microscopy: Fluorescence Lifetime Imaging (FLIM)

20. Widefield Fluorescence Lifetime Imaging with Multi-Anode Detectors

Roland Hartig, Yury Prokazov, Evgeny Turbin, and Werner Zuschratter

21. Global Analysis of FRET- FLIM Data in Live Plant Cells

Sergey P. Laptenok, Joris J. Snellenburg, Christoph A. Bьcherl, Kai R. Konrad, and Jan Willem Borst

22. Time-Resolved Fluorescence Anisotropy Imaging

Klaus Suhling, James Levitt, and Pei-Hua Chung

23. Multi-Modal Fluo

Автор: Pietro Ferraro; Adam Wax; Zeev Zalevsky
Название: Coherent Light Microscopy
ISBN: 3642267106 ISBN-13(EAN): 9783642267109
Издательство: Springer
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Описание: This book illustrates the strengths and capabilities of developing techniques in optical coherent microscopy. The areas of application of this technique are in biomedicine, medicine, life sciences, nanotechnology and materials sciences.

Автор: P. Michael Conn
Название: Laser Capture in Microscopy and Microdissection,356
ISBN: 0121822591 ISBN-13(EAN): 9780121822590
Издательство: Elsevier Science
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Описание: Part of a series which contains material useful for researchers in various fields of life sciences. This title includes basic principles, specialized uses, and genetic applications; LCM and its application in genomics and proteomics; and, fluorescence in situ hybridization of LCM isolated nuclei from paraffin sections.

Автор: Goerg H. Michler
Название: Electron Microscopy of Polymers
ISBN: 364207166X ISBN-13(EAN): 9783642071669
Издательство: Springer
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Цена: 16977.00 р.
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Описание: The study of polymers by electron microscopy (EM) needs special techniques, precautions and preparation methods, including ultramicrotomy. General characteristics of the different techniques of EM, including scanning force microscopy, are given in this hands-on book.