Автор: Dennison C. Название: A Guide to Protein Isolation ISBN: 1402012241 ISBN-13(EAN): 9781402012242 Издательство: Springer Рейтинг: Цена: 9782.00 р. 13974.00-30% Наличие на складе: Есть (1 шт.) Описание: Traffic exists on a different "level" - it is dependent upon the existence of motor vehicles but the physics and mathematics of traffic can be adequately addressed by considering motor vehicles as mobile "blobs", with no consideration of how they become mobile.
Автор: Walls Название: Protein Chromatography ISBN: 1607619121 ISBN-13(EAN): 9781607619123 Издательство: Springer Рейтинг: Цена: 23058.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: A prerequisite for elucidating the structure and function of any protein is the prior purification of that protein. This necessity has led to the development of many purification schemes and chromatographic methods for the isolation of native proteins from complex sources. In Protein Chromatography: Methods and Protocols, leading researchers present clear protocol-style chapters that are suitable for newcomers and experts alike. The book opens with vital topics in protein biochemistry, addressing such areas as protein stability and storage, avoiding proteolysis during chromatography, protein quantitation methods including immuno-qPCR, and the contrasting challenges that microfluidics and scale-up production pose to the investigator, and then it segues into key methods involving the generation and purification of recombinant proteins through recombinant antibody production and the tagging of proteins, amongst other means, as well as many variations on classic techniques such as ion-exchange and immunoaffinity chromatography. Written in the highly successful Methods in Molecular Biology™ series format, protocols chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Protein Chromatography: Methods and Protocols will greatly aid scientists in establishing these essential techniques in their own laboratories and furthering our understanding of the many imperative functions of proteins.
Описание: This unique volume reviews the beautiful architectures and varying mechanical actions of the set of specialized cellular proteins called molecular chaperones, which provide essential kinetic assistance to processes of protein folding and unfolding in the cell.
Описание: This thesis focuses on the study of interactions between protein and peptides and their potential applications in cell imaging and nanoparticle surface modification. Drawing inspiration from naturally occurring coiled-coil binding pairs, it proposes a novel covalent peptide tag and probe system, based on the concept of “affinity guided covalent conjugation.” This newly established methodology provides complementary resolution to protein labeling, imaging and trafficking. By systematically investigating the coordination interaction between protein and quantum dots using various engineered protein ligands, this thesis proposes a general rule for protein self-assembly on the surface of quantum dots and reports a revolutionized nanobelt protein in accordance with this rule. It is an extraordinary example of interdisciplinary research, providing answers to real-life biological problems from a chemistry perspective.
Автор: James R. Swartz Название: Cell-Free Protein Expression ISBN: 3642639399 ISBN-13(EAN): 9783642639395 Издательство: Springer Рейтинг: Цена: 14365.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Motivated by an escalating need for efficient protein synthesis and empowered by readily accessible cell-free protein synthesis kits, the technology is expanding both in the range of feasible proteins and in the ways that proteins can be labeled and modified.
Автор: Fan-Gang Tseng; Tuhin Subhra Santra Название: Essentials of Single-Cell Analysis ISBN: 3662491168 ISBN-13(EAN): 9783662491164 Издательство: Springer Рейтинг: Цена: 18167.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This book provides an overview of single-cellisolation, separation, injection,lysis and dynamics analysis as well as a study of their heterogeneity usingdifferent miniaturized devices.
Описание: Inthis thesis single-molecule fluorescence resonance energy transfer(FRET)spectroscopy was used to study the folding of a protein that belongsto the large and important family of repeat proteins.
Автор: Bert, van Duijn; Anneke Wiltink Название: Signal Transduction — Single Cell Techniques ISBN: 3642489761 ISBN-13(EAN): 9783642489761 Издательство: Springer Рейтинг: Цена: 16979.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: A variety of powerful techniques for monitoring and analysing events during signal transduction at the single cell level are described in this lab manual. A main section of the book presents protocols on fluorescence techniques such as flow cytometry, microfluorescence, ion imaging and confocal microscopy.
Автор: Xiangdong Wang Название: Single Cell Sequencing and Systems Immunology ISBN: 9401797528 ISBN-13(EAN): 9789401797528 Издательство: Springer Рейтинг: Цена: 19564.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: The volume focuses on the genomics, proteomics, metabolomics, and bioinformatics of a single cell, especially lymphocytes and on understanding the molecular mechanisms of systems immunology. Based on the author’s personal experience, it provides revealing insights into the potential applications, significance, workflow, comparison, future perspectives and challenges of single-cell sequencing for identifying and developing disease-specific biomarkers in order to understand the biological function, activation and dysfunction of single cells and lymphocytes and to explore their functional roles and responses to therapies. It also provides detailed information on individual subgroups of lymphocytes, including cell characters, function, surface markers, receptor function, intracellular signals and pathways, production of inflammatory mediators, nuclear receptors and factors, omics, sequencing, disease-specific biomarkers, bioinformatics, networks and dynamic networks, their role in disease and future prospects. Dr. Xiangdong Wang is a Professor of Medicine, Director of Shanghai Institute of Clinical Bioinformatics, Director of Fudan University Center for Clinical Bioinformatics, Director of the Biomedical Research Center of Zhongshan Hospital, Deputy Director of Shanghai Respiratory Research Institute, Shanghai, China.
Автор: Richard R Burgess Название: Guide to Protein Purification,436 ISBN: 0123749786 ISBN-13(EAN): 9780123749789 Издательство: Elsevier Science Рейтинг: Цена: 12127.00 р. Наличие на складе: Поставка под заказ.
Описание: Examines some of the most reliable, robust methods for researchers in biochemistry, molecular and cell biology, genetics, pharmacology and biotechnology. This book relates how these traditional methods connect to the explosive advancements in the field. It includes chapters on both common and less-common relevant techniques.
Автор: Bonner, Philip L.r. Название: Protein purification ISBN: 0415385113 ISBN-13(EAN): 9780415385114 Издательство: Taylor&Francis Рейтинг: Цена: 6278.00 р. Наличие на складе: Поставка под заказ.
Описание: A guide to the major techniques, including non-affinity absorption techniques, affinity procedures, non-absorption techniques and methods for monitoring protein purity. It also includes an overview of protein strategy and equipment, followed by discussions and examples of each technique and its applications.
