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Atomic Force Microscopy, Pier Carlo Braga; Davide Ricci


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Автор: Pier Carlo Braga; Davide Ricci
Название:  Atomic Force Microscopy
ISBN: 9781489939319
Издательство: Springer
Классификация:
ISBN-10: 1489939318
Обложка/Формат: Soft cover
Страницы: 394
Вес: 0.60 кг.
Дата издания: 11.08.2013
Серия: Methods in Molecular Biology
Язык: English
Издание: Softcover reprint of
Иллюстрации: Xiv, 394 p.
Размер: 229 x 152 x 21
Читательская аудитория: Professional & vocational
Основная тема: Biochemistry, general
Подзаголовок: Biomedical Methods and Applications
Ссылка на Издательство: Link
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Поставляется из: Германии
Описание: The natural, biological, medical, and related sciences would not be what they are today without the microscope. After the introduction of the optical microscope, a second breakthrough in morphostructural surface analysis occurred in the 1940s with the development of the scanning electron microscope (SEM), which, instead of light (i. e. , photons) and glass lenses, uses electrons and electromagnetic lenses (magnetic coils). Optical and scanning (or transmission) electron microscopes are called “far-field microscopes” because of the long distance between the sample and the point at which the image is obtained in comparison with the wavelengths of the photons or electrons involved. In this case, the image is a diffraction pattern and its resolution is wavelength limited. In 1986, a completely new type of microscopy was proposed, which, without the use of lenses, photons, or electrons, directly explores the sample surface by means of mechanical scanning, thus opening up unexpected possibilities for the morphostructural and mechanical analysis of biological specimens. These new scanning probe microscopes are based on the concept of near-field microscopy, which overcomes the problem of the limited diffraction-related resolution inherent in conventional microscopes. Located in the immediate vicinity of the sample itself (usually within a few nanometers), the probe records the intensity, rather than the interference signal, thus significantly improving resolution. Since the most we- known microscopes of this type operate using atomic forces, they are frequently referred to as atomic force microscopes (AFMs).
Дополнительное описание: The Basics of Atomic Force Microscopy.- How the Atomic Force Microscope Works.- Imaging Methods in Atomic Force Microscopy.- Recognizing and Avoiding Artifacts in AFM Imaging.- Advanced Biosensing Using Micromechanical Cantilever Arrays.- Morphostructural



Electron Microscopy of Polymers

Автор: Michler G.H.
Название: Electron Microscopy of Polymers
ISBN: 3540363505 ISBN-13(EAN): 9783540363507
Издательство: Springer
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Описание: There are many books on electron microscopy, however, the study of polymers by EM needs special techniques, precautions and preparation methods, including ultramicrotomy. General characteristics of the different techniques of EM, including scanning force microscopy (AFM), are given. Application of these techniques to study morphology and also properties, particularly micromechanical properties, is described in detail. Examples from all classes of polymers are presented.

Light microscopy

Автор: Yolanda Markaki
Название: Light microscopy
ISBN: 1493968084 ISBN-13(EAN): 9781493968084
Издательство: Springer
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Цена: 19564.00 р.
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Описание:

Preface...
Table of Contents...
Contributing Authors...

1. Introduction to Modern Methods in Light Microscopy


Part I Advanced Fluorescence Microscopy: Sample Preparation, Fluorophores and Model Systems

2. Three-Dimensional Live Imaging of Filamentous Fungi with Light Sheet-Based Fluorescence Microscopy (LSFM)
Francesco Pampaloni, Laura Knuppertz, Andrea Hamann, Heinz D. Osiewacz, and Ernst H.K. Stelzer

3. Light-Sheet Fluorescence Microscopy: Chemical Clearing and Labeling Protocols for Ultramicroscopy
Nina Jдhrling, Klaus Becker, Saiedeh Saghafi, and Hans-Ulrich Dodt

4. Two-Photon Intravital Microscopy Animal Preparation Protocol to Study Cellular Dynamics in Pathogenesis
Erinke van Grinsven, Chloe Prunier, Nienke Vrisekoop, and Laila Ritsma

5. Imaging of Brain Slices with a Genetically Encoded Voltage IndicatorPeter Quicke, Samuel J. Barnes, and Thomas Knцpfel

6. FRET Microscopy for Real-Time Visualization of Second Messengers in Living Cells
Axel E. Kraft and Viacheslav O. Nikolaev

7. Imaging the Dynamics of Cell Wall Polymer Deposition in the Unicellular Model Plant, Penium margaritaceum
David Domozych, Anna Lietz, Molly Patten, Emily Singer, Berke Tinaz, and Sandra C. Raimundo

8. Targeted Ablation Using Laser Nanosurgery
Naga Venkata Gayathri Vegesna, Paolo Ronchi, Sevi Durdu, Stefan Terjung, and Rainer Pepperkok

Part II Super and High-Resolution Optical Imaging

9. Sample Preparation and Choice of Fluorophores for Single and Dual Color Photo-Activated Localization Microscopy (PALM) with Bacterial Cells
Juri N. Bach, Giacomo Giacomelli, and Marc Bramkamp

10. STED Imaging in Drosophila Brain Slices
Sandra Fendl, Jesъs Pujol-Marti, Joel Ryan, Alexander Borst, and Robert Kasper

11. Two-Color Total Internal Refection Fluorescence Microscopy of Exocytosis in Endocrine Cells
Adam J. Trexler and Justin W. Taraska

12. Optical Coherence Microscopy
Rainer A. Leitgeb

Part III Quantitative and Computational Image Analysis

13. Designing Image Analysis Pipelines in Light Microscopy: A Rationale Approach
Ignacio Arganda-Carreras and Philippe Andrey

14. Automated Analysis of Intracellular Dynamic Processes
Yao Yao, Ihor Smal, Ilya Grigoriev, Maud Martin, Anna Akhmanova, and Erik Meijering

15. Quantitative Image Analysis of Single-Molecule mRNA Dynamics in Living Cells
Josй Rino, Ana C. de Jesus, and Maria Carmo-Fonseca

16. Analysis of Protein Kinetics using Fluorescence Recovery After Photobleaching (FRAP)
Nickolaos-Nikiforos Giakoumakis, Maria Anna Rapsomaniki, and Zoi Lygerou

17. Fluorescence Based High-Throughput and Targeted Image Acquisition and Analysis for Phenotypic Screening
Manuel Gunkel, Jan Philipp Eberle, and Holger Erfle

Confocal Microscopy for Biologists

Автор: Hibbs Alan R.
Название: Confocal Microscopy for Biologists
ISBN: 0306484684 ISBN-13(EAN): 9780306484681
Издательство: Springer
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Описание: There has been a great upsurge in interest in light microscopy in recent years due to the advent of a number of significant advances in microscopy, one of the most important of which is confocal microscopy.

Introduction to Microscopy by Means of Light, Electrons, X Rays, or Acoustics

Автор: Theodore G. Rochow; Paul A. Tucker
Название: Introduction to Microscopy by Means of Light, Electrons, X Rays, or Acoustics
ISBN: 148991515X ISBN-13(EAN): 9781489915153
Издательство: Springer
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Описание: The authors are greatly indebted to the College of Textiles of North Carolina State University at Raleigh for support from the administration there for typing, word processing, stationery, mailing, drafting diagrams, and general assistance.

Biological Specimen Preparation for Transmission Electron Microscopy

Автор: Glauert Audrey M., Lewis Peter R.
Название: Biological Specimen Preparation for Transmission Electron Microscopy
ISBN: 0691600155 ISBN-13(EAN): 9780691600154
Издательство: Wiley
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Цена: 9821.00 р.
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Описание:

This book contains all the necessary information and advice for anyone wishing to obtain electron micrographs showing the most accurate ultrastructural detail "in thin sections" of any type of biological specimen.

The guidelines for the choice of preparative methods are based on an extensive survey of current laboratory practice. For the first time, in a textbook of this kind, the molecular events occurring during fixation and embedding are analysed in detail. The reasons for choosing particular specimen preparation methods are explained and guidance is given on how to modify established techniques to suit individual requirements.

All the practical methods advocated are clearly described, with accompanying tables and the results obtainable are illustrated with many electron micrographs.

Portland Press Series: "Practical Methods in Electron Microscopy," Volume 17, Audrey M. Glauert, Editor

Originally published in 1999.

The Princeton Legacy Library uses the latest print-on-demand technology to again make available previously out-of-print books from the distinguished backlist of Princeton University Press. These paperback editions preserve the original texts of these important books while presenting them in durable paperback editions. The goal of the Princeton Legacy Library is to vastly increase access to the rich scholarly heritage found in the thousands of books published by Princeton University Press since its founding in 1905.

Electron Microscopy of Polymers

Автор: Goerg H. Michler
Название: Electron Microscopy of Polymers
ISBN: 364207166X ISBN-13(EAN): 9783642071669
Издательство: Springer
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Цена: 16977.00 р.
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Описание: The study of polymers by electron microscopy (EM) needs special techniques, precautions and preparation methods, including ultramicrotomy. General characteristics of the different techniques of EM, including scanning force microscopy, are given in this hands-on book.

Laser Capture in Microscopy and Microdissection,356

Автор: P. Michael Conn
Название: Laser Capture in Microscopy and Microdissection,356
ISBN: 0121822591 ISBN-13(EAN): 9780121822590
Издательство: Elsevier Science
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Описание: Part of a series which contains material useful for researchers in various fields of life sciences. This title includes basic principles, specialized uses, and genetic applications; LCM and its application in genomics and proteomics; and, fluorescence in situ hybridization of LCM isolated nuclei from paraffin sections.


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