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Gene Targeting Protocols, Eric B. Kmiec


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Автор: Eric B. Kmiec
Название:  Gene Targeting Protocols
ISBN: 9781489941589
Издательство: Springer
Классификация:
ISBN-10: 1489941584
Обложка/Формат: Soft cover
Страницы: 233
Вес: 0.39 кг.
Дата издания: 14.08.2013
Серия: Methods in Molecular Biology
Язык: English
Издание: Softcover reprint of
Иллюстрации: Xv, 233 p.
Размер: 230 x 153 x 19
Читательская аудитория: Professional & vocational
Основная тема: Human Genetics
Ссылка на Издательство: Link
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Поставляется из: Германии
Описание: The potential now exists in many experimental systems to transfer a cloned, modified gene back into the genome of the host organism. In the ideal situation, the cloned gene is returned to its homologous location in the genome and becomes inserted at the target locus. This process is a controlled means for the repair of DNA damage and ensures accurate chromosome disjunction during meiosis. The paradigm for thinking about the mechanism of this p- cess has emerged primarily from two sources: (1) The principles of reaction mechanics have come from detailed biochemical analyses of the RecA protein purified from Escherichia coli; and (2) the principles of information transfer have been derived from genetic studies carried out in bacteriophage and fungi. A compelling picture of the process of homologous pairing and DNA strand exchange has been influential in directing investigators interested in gene t- geting experiments. The ability to find and pair homologous DNA molecules enables ac- rate gene targeting and is the central phenomenon underlying genetic recombi- tion. Biochemically, the overall process can be thought of as a series of steps in a reaction pathway whereby DNA molecules are brought into homologous register, the four-stranded Holliday structure intermediate is formed, hete- duplex DNA is extended, and DNA strands are exchanged. Not much is known about the biochemical pathway leading to homologous recombination in euka- otes.
Дополнительное описание: Nucleic Acid Transfer Using Cationic Lipids.- Optimizing Polyethylenimine-Based Gene Transfer into Mammalian Brain for Analysis of Promoter Regulation and Protein Function.- Gene Transfer and Drug Delivery by Electronic Pulse Delivery.- Strategies for Imp



Gene therapy protocols: Vol 2 design and characterization of gene transfer vectors

Автор: LeDoux, Joseph (Ed.)
Название: Gene therapy protocols: Vol 2 design and characterization of gene transfer vectors
ISBN: 160327247X ISBN-13(EAN): 9781603272476
Издательство: Springer
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Цена: 23757.00 р.
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Описание: The field of gene therapy has undergone remarkable advances, promising to impact human healthcare in the 21st century. This book provides methods for the processing and characterization of viral and non-viral gene transfer vectors, as well as promising approaches to design vectors for efficient, targeted and regulated gene delivery and expression.

Protein Targeting Protocols

Автор: Mark van der Giezen
Название: Protein Targeting Protocols
ISBN: 1617377279 ISBN-13(EAN): 9781617377273
Издательство: Springer
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Цена: 21661.00 р.
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Описание: In this updated volume, experts from around the world provide the latest protocols for isolating different organelles and the localization of particular proteins using a variety of methods such as light, confocal, and electron microscopy. Emphasis is placed on protein targeting of cellular compartments in both prokaryotic and eukaryotic systems.

Treatment of Neurodevelopmental Disorders: Targeting Neurobiological Mechanisms

Автор: Hagerman Randi Jenssen, Hendren Robert L.
Название: Treatment of Neurodevelopmental Disorders: Targeting Neurobiological Mechanisms
ISBN: 019993780X ISBN-13(EAN): 9780199937806
Издательство: Oxford Academ
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Цена: 16236.00 р.
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Описание: This book brings advances in genetics, neurobiology, and psychopharmacology to the clinic to enhance treatment for neurodevelopmental disorders. Significant progress has been made in identifying the neurobiological mechanisms of several disorders and targeted treatments are modifying the outcome of these disorders.

Targeting of Drugs 5

Автор: Gregory Gregoriadis; Brenda McCormack
Название: Targeting of Drugs 5
ISBN: 1461379431 ISBN-13(EAN): 9781461379430
Издательство: Springer
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Цена: 6986.00 р.
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Описание: Proceedings of a NATO ASI held in Cape Sounion, Greece, June 24-July 5, 1995

Microbial Gene Essentiality: Protocols and Bioinformatics

Автор: Andrei L. Osterman; Svetlana Y. Gerdes
Название: Microbial Gene Essentiality: Protocols and Bioinformatics
ISBN: 1617375292 ISBN-13(EAN): 9781617375293
Издательство: Springer
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Цена: 23058.00 р.
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Описание: This book contains a comprehensive collection of experimental and computational strategies and techniques for microbial genome-scale essentiality studies, developed and presented by the leading groups in the field. It contains detailed description of the procedures, discussion of potential difficulties and failures.

Gene Knockout Protocols

Автор: Martin J. Tymms; Ismail Kola
Название: Gene Knockout Protocols
ISBN: 1617370800 ISBN-13(EAN): 9781617370809
Издательство: Springer
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Цена: 24456.00 р.
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Описание: As the major task of sequencing the human genome is near completion and full complement of human genes are catalogued, attention will be focused on the ultimate goal: to understand the normal biological functions of these genes, and how alterations lead to disease states.

Gene Therapy Protocols

Автор: Joseph LeDoux
Название: Gene Therapy Protocols
ISBN: 1627039643 ISBN-13(EAN): 9781627039642
Издательство: Springer
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Цена: 19564.00 р.
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Описание: In Gene Therapy Protocols, Volumes 1 & 2, internationally recognized investigators describe cutting-edge laboratory techniques for the study of Production and In Vivo Applications of Gene Transfer Vectors (Volume 1) and Design and Characterization of Gene Transfer Vectors (Volume 2). The field of gene therapy has undergone remarkable advances, promising to impact human healthcare significantly in the twenty-first century. Today’s technologies can deliver genetic material safely and effectively to cells to slow or halt the progression of disease, and to help repair or regenerate damaged or lost tissues. In this second volume of Gene Therapy Protocols: Design and Characterization of Gene Transfer Vectors, readers will find a comprehensive resource of current and emerging methods for the processing and characterization of viral and non-viral gene transfer vectors, as well as promising approaches to design vectors for efficient, targeted and regulated gene delivery and expression. This second volume of the new and completely revised third edition of Gene Therapy Protocols will prove a necessary tool for graduate students and postdoctoral fellows and invaluable to basic and clinical researchers in both industry and academia.


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