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Epithelial Cell Culture Protocols, Scott H. Randell; M. Leslie Fulcher


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Автор: Scott H. Randell; M. Leslie Fulcher
Название:  Epithelial Cell Culture Protocols
ISBN: 9781493956791
Издательство: Springer
Классификация:
ISBN-10: 1493956795
Обложка/Формат: Paperback
Страницы: 469
Вес: 0.91 кг.
Дата издания: 23.08.2016
Серия: Methods in Molecular Biology
Язык: English
Размер: 254 x 178 x 25
Основная тема: Life Sciences
Ссылка на Издательство: Link
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Поставляется из: Германии
Описание: In its 2nd edition, this Methods in Molecular Biology (TM) volume provides a cross section of up-to-date culture protocols for the most studied cell systems and supporting technologies. Includes materials lists, step-by-step protocols and troubleshooting tips.


Epithelial Transport Physiology

Автор: George A. Gerencser
Название: Epithelial Transport Physiology
ISBN: 1627038434 ISBN-13(EAN): 9781627038430
Издательство: Springer
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Цена: 28732.00 р.
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Описание: The theme of this book is epithelial transport of molecules and ions, which includes the regulation of these mechanisms either directly, through secondary messengers, or via nuclear commands. Contributors were selected for their outstanding accomplishments.

Basic Cell Culture Protocols

Автор: Cheryl D. Helgason; Cindy L. Miller
Название: Basic Cell Culture Protocols
ISBN: 1627039309 ISBN-13(EAN): 9781627039307
Издательство: Springer
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Цена: 27951.00 р.
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Описание: Culture of Primary Adherent Cells and a Continuously Growing Nonadherent Cell Line Cheryl D. Helgason Detection of Mycoplasma Contaminations Cord C. Uphoff and Hans G. Drexler Eradication of Mycoplasma Contaminations Cord C. Uphoff and Hans G. Drexler Authentication of Scientific Human Cell Lines: Easy-to-Use DNA Fingerprinting Wilhelm G. Dirks and Hans G. Drexler Cytogenetic Analysis of Cell Lines Roderick A. F. MacLeod and Hans G. Drexler Human and Mouse Hematopoietic Colony-Forming Cell Assays Cindy L. Miller and Becky Lai Isolation and Culture of Murine Macrophages John Q. Davies and Siamon Gordon Isolation and Culture of Human Macrophages John Q. Davies and Siamon Gordon Development of T-Lymphocytes in Mouse Fetal Thymus Organ Culture Tomoo Ueno, Cunlan Liu, Takeshi Nitta, and Yousuke Takahama In Vitro Generation of Lymphocytes From Embryonic Stem Cells Renйe F. de Pooter, Sarah K. Cho, and Juan Carlos Zъсiga-Pflьcker Hematopoietic Development of Human Embryonic Stem Cells in Culture Xinghui Tian and Dan S. Kaufman Generation of Murine Stromal Cell Lines: Models for the Microenvironment of the Embryonic Mouse Aorta-Gonads-Mesonephros Region Robert A. J. Oostendorp, Kirsty Harvey, and Elaine A. Dzierzak Culture of Human and Mouse Mesenchymal Cells Emer Clarke Isolation, Purification, and Cultivation of Murine and Human Keratinocytes Frizell L. Vaughan and Ludmila I. Bernstam Isolation and Culture of Primary Human Hepatocytes Edward L. LeCluyse, Eliane Alexandre, Geraldine A. Hamilton, Catherine Viollon-Abadie, D. James Coon, Summer Jolley, and Lysiane Richert Primary Kidney Proximal Tubule Cells Mary Taub Enzymatic Dissociation and Culture of Normal Human Mammary Tissue to Detect ProgenitorActivity John Stingl, Joanne T. Emerman, and Connie J. Eaves Generation and Differentiation of Neurospheres From Murine Embryonic Day 14 Central Nervous System Tissue Sharon A. Louis and Brent A. Reynolds Isolation and Culture of Skeletal Muscle Myofibers as a Means to Analyze Satellite Cells Gabi Shefer and Zipora Yablonka-Reuveni Adult Ventricular Cardiomyocytes: Isolation and Culture Klaus-Dieter Schlьter and Daniela Schreiber Isolation and Culture of Primary Endothelial Cells Bruno Larrivйe and Aly Karsan Studying Leukocyte Rolling and Adhesion In Vitro Under Flow Conditions Susan L. Cuvelier and Kamala D. Patel Isolation and Characterization of Side Population Cells Margaret A. Goodell, Shannon McKinney-Freeman, and Fernando D. Camargo Scalable Production of Embryonic Stem Cell-Derived Cells Stephen M. Dang and Peter W. Zandstra Index

Basic Cell Culture Protocols

Автор: Helgason Cheryl D., Miller Cindy
Название: Basic Cell Culture Protocols
ISBN: 1588292843 ISBN-13(EAN): 9781588292841
Издательство: Springer
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Цена: 27951.00 р.
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Описание: In this fully revised edition of an established classic, expert researchers and clinicians describe in step-by-step detail updated techniques for the isolation and growth of major primary cell types, such as kidney proximal tubule cells, hepatocytes, keratinocytes, and cardiomyocytes. The authors offer readily reproducible new methods for the differentiation of embryonic stem (ES) cells into various hematopoietic cell types, for fetal thymic organ culture, and for the isolation and culture of specialized cell types, such as mammary progenitor cells, skeletal muscle myofibers, mesenchymal cells, neural stem cells, hematopoietic cells, stromal cell lines, and endothelial cells. Additional chapters describe new techniques (leukocyte rolling, isolation of side-population cells, and scalable production of ES-derived cells) and detail quality control methods for cell lines (detection and elimination of mycoplasma, DNA fingerprinting, and cytogenetic analysis).

Epithelial Transport

Автор: N.K. Wills; L. Reuss; S.A. Lewis
Название: Epithelial Transport
ISBN: 0412434008 ISBN-13(EAN): 9780412434006
Издательство: Springer
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Цена: 28734.00 р.
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Описание: Building from general principles, the authors clearly explain the fundamental role of epithelia in plasma electrolyte and water balance. Emphasis is placed on experimental approaches and methodology. A comprehensive glossary of terms is included.

Epithelial Organization and Development

Автор: T.P. Fleming
Название: Epithelial Organization and Development
ISBN: 0412398907 ISBN-13(EAN): 9780412398902
Издательство: Springer
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Цена: 28734.00 р.
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Описание: Epithelia are one of the commonest tissue types in the animal kingdom. An important theme is the way in which epithelial cells differentiate to specialized tissue - reversal of this process occurs when cells become tumorigenic.

Mesenchymal-Epithelial Interactions in Neural Development

Автор: Joachim R. Wolff; Jobst Sievers; Martin Berry
Название: Mesenchymal-Epithelial Interactions in Neural Development
ISBN: 3642718396 ISBN-13(EAN): 9783642718397
Издательство: Springer
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Цена: 18167.00 р.
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Описание: Proceedings of the NATO Advanced Research Workshop on Mesenchymal-Epithelial Interaction in Neural Development held at West-Berlin,FRG, March 17-21, 1986

Epithelial—Mesenchymal Interactions in Cancer

Автор: Itzhak D. Goldberg; Eliot M. Rosen
Название: Epithelial—Mesenchymal Interactions in Cancer
ISBN: 3034898932 ISBN-13(EAN): 9783034898935
Издательство: Springer
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Цена: 14673.00 р.
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Plant Cell Culture Protocols

Автор: V?ctor M. Loyola-Vargas; Neftal? Ochoa-Alejo
Название: Plant Cell Culture Protocols
ISBN: 1493962353 ISBN-13(EAN): 9781493962358
Издательство: Springer
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Цена: 16070.00 р.
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Описание: Cell culture methodologies have become standard procedures in most plant laboratories. Currently, facilities for in vitro cell cultures are found in practically every plant biology laboratory, serving different purposes since tissue culture has turned into a basic asset for modern biotechnology, from the fundamental biochemical aspects to the massive propagation of selected individuals. “Plant Cell Culture Protocols, Third Edition is divided into five convenient sections that cover topics from general methodologies, such as culture induction, growth and viability evaluation, statistical analysis and contamination control, to highly specialized techniques, such as clonal propagation, haploid production, somatic embryogenesis, organelle transformation. The volume concludes with a section on the laborious process of measuring the epigenetics changes in tissue cultures.”Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Plant Cell Culture Protocols, Third Edition seeks to serve both professionals and novices with its guide to the most common and applicable techniques and methods for plant tissue and cell culture.

Human Cell Culture Protocols

Автор: Ragai R. Mitry; Robin D. Hughes
Название: Human Cell Culture Protocols
ISBN: 1493962086 ISBN-13(EAN): 9781493962082
Издательство: Springer
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Цена: 18167.00 р.
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Описание: Human cell culture is not a new topic, but the development of new molecular techniques and reagents which can be used to investigate cell function and the responsible intracellular mechanisms make it a continuing requirement. This third edition of Human Cell Culture Protocols expands upon the previous editions with current, detailed protocols for the isolation and culture of a range of primary cells from human tissues. With new chapters on pancreatic cells needed for basic studies on the pathogenesis of diabetes and for their application for islet transplantation, the book also delves into protocols for hepatocytes, skin cells, lung cells, parathyroid cells, gastric cells, renal cells, adipocytes, ovarian cells, bone cells, vascular smooth muscle cells, vascular endothelial cells, regulatory T cells, blood mononuclear cells, as well as new techniques being applied to human cell culture, particularly the use of biocompatible scaffolds to grow cells, the in vitro use of laser microdissection to isolate cells from culture, and automated cell culture. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Human Cell Culture Protocols, Third Edition makes it possible for a worker with basic cell culture training, whether in the fields of cell biology, gene therapy, and cell transplantation, to prepare cell cultures of the specific cell type necessary to forward their vital research.

Neuronal Cell Culture: Methods and Protocols (2013) ( Methods in Molecular Biology #1078 )

Автор: Amini, Shohreh (Editor), White, Martyn K (Editor)
Название: Neuronal Cell Culture: Methods and Protocols (2013) ( Methods in Molecular Biology #1078 )
ISBN: 1627036393 ISBN-13(EAN): 9781627036399
Издательство: Springer
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Цена: 20962.00 р.
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Описание: This book details the latest aspects of the culture of neural cells and explains the practical and theoretical considerations of the techniques involved. It features step-by-step, readily reproducible laboratory protocols and expert implementation advice.


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