Human Cell Culture Protocols, Ragai R. Mitry; Robin D. Hughes
Автор: Turksen Название: Human Embryonic Stem Cell Protocols ISBN: 1607613689 ISBN-13(EAN): 9781607613688 Издательство: Springer Рейтинг: Цена: 14673.00 р. 20962.00-30% Наличие на складе: Есть (1 шт.) Описание: Controversy surrounds the study of human embryonic stem cells. In this book, respected researchers expand upon the popular first edition and describe in detail their most useful techniques for the molecular and cellular manipulation of these intriguing cells.
Автор: V?ctor M. Loyola-Vargas; Neftal? Ochoa-Alejo Название: Plant Cell Culture Protocols ISBN: 1493962353 ISBN-13(EAN): 9781493962358 Издательство: Springer Рейтинг: Цена: 16070.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Cell culture methodologies have become standard procedures in most plant laboratories. Currently, facilities for in vitro cell cultures are found in practically every plant biology laboratory, serving different purposes since tissue culture has turned into a basic asset for modern biotechnology, from the fundamental biochemical aspects to the massive propagation of selected individuals. “Plant Cell Culture Protocols, Third Edition is divided into five convenient sections that cover topics from general methodologies, such as culture induction, growth and viability evaluation, statistical analysis and contamination control, to highly specialized techniques, such as clonal propagation, haploid production, somatic embryogenesis, organelle transformation. The volume concludes with a section on the laborious process of measuring the epigenetics changes in tissue cultures.”Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Plant Cell Culture Protocols, Third Edition seeks to serve both professionals and novices with its guide to the most common and applicable techniques and methods for plant tissue and cell culture.
Автор: Helgason Cheryl D., Miller Cindy Название: Basic Cell Culture Protocols ISBN: 1588292843 ISBN-13(EAN): 9781588292841 Издательство: Springer Рейтинг: Цена: 27951.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: In this fully revised edition of an established classic, expert researchers and clinicians describe in step-by-step detail updated techniques for the isolation and growth of major primary cell types, such as kidney proximal tubule cells, hepatocytes, keratinocytes, and cardiomyocytes. The authors offer readily reproducible new methods for the differentiation of embryonic stem (ES) cells into various hematopoietic cell types, for fetal thymic organ culture, and for the isolation and culture of specialized cell types, such as mammary progenitor cells, skeletal muscle myofibers, mesenchymal cells, neural stem cells, hematopoietic cells, stromal cell lines, and endothelial cells. Additional chapters describe new techniques (leukocyte rolling, isolation of side-population cells, and scalable production of ES-derived cells) and detail quality control methods for cell lines (detection and elimination of mycoplasma, DNA fingerprinting, and cytogenetic analysis).
Автор: Turksen Kursad Название: Human Embryonic Stem Cell Protocols ISBN: 1588294978 ISBN-13(EAN): 9781588294975 Издательство: Springer Рейтинг: Цена: 14947.00 р. Наличие на складе: Поставка под заказ.
Описание: A comprehensive collection of diverse techniques for the molecular and cellular manipulation of human embryonic stem (hES) cells. These readily reproducible methods have been optimized for the derivation, characterization, and differentiation of hES cells, with special attention given to regenerative medicine applications. A companion CD provides color versions of all illustrations in the book. The protocols follow the successful Methods in Molecular Biologyв„ў series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Описание: This book details the latest aspects of the culture of neural cells and explains the practical and theoretical considerations of the techniques involved. It features step-by-step, readily reproducible laboratory protocols and expert implementation advice.
Автор: Cheryl D. Helgason; Cindy L. Miller Название: Basic Cell Culture Protocols ISBN: 1627039309 ISBN-13(EAN): 9781627039307 Издательство: Springer Рейтинг: Цена: 27951.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Culture of Primary Adherent Cells and a Continuously Growing Nonadherent Cell Line Cheryl D. Helgason Detection of Mycoplasma Contaminations Cord C. Uphoff and Hans G. Drexler Eradication of Mycoplasma Contaminations Cord C. Uphoff and Hans G. Drexler Authentication of Scientific Human Cell Lines: Easy-to-Use DNA Fingerprinting Wilhelm G. Dirks and Hans G. Drexler Cytogenetic Analysis of Cell Lines Roderick A. F. MacLeod and Hans G. Drexler Human and Mouse Hematopoietic Colony-Forming Cell Assays Cindy L. Miller and Becky Lai Isolation and Culture of Murine Macrophages John Q. Davies and Siamon Gordon Isolation and Culture of Human Macrophages John Q. Davies and Siamon Gordon Development of T-Lymphocytes in Mouse Fetal Thymus Organ Culture Tomoo Ueno, Cunlan Liu, Takeshi Nitta, and Yousuke Takahama In Vitro Generation of Lymphocytes From Embryonic Stem Cells Renйe F. de Pooter, Sarah K. Cho, and Juan Carlos Zъсiga-Pflьcker Hematopoietic Development of Human Embryonic Stem Cells in Culture Xinghui Tian and Dan S. Kaufman Generation of Murine Stromal Cell Lines: Models for the Microenvironment of the Embryonic Mouse Aorta-Gonads-Mesonephros Region Robert A. J. Oostendorp, Kirsty Harvey, and Elaine A. Dzierzak Culture of Human and Mouse Mesenchymal Cells Emer Clarke Isolation, Purification, and Cultivation of Murine and Human Keratinocytes Frizell L. Vaughan and Ludmila I. Bernstam Isolation and Culture of Primary Human Hepatocytes Edward L. LeCluyse, Eliane Alexandre, Geraldine A. Hamilton, Catherine Viollon-Abadie, D. James Coon, Summer Jolley, and Lysiane Richert Primary Kidney Proximal Tubule Cells Mary Taub Enzymatic Dissociation and Culture of Normal Human Mammary Tissue to Detect ProgenitorActivity John Stingl, Joanne T. Emerman, and Connie J. Eaves Generation and Differentiation of Neurospheres From Murine Embryonic Day 14 Central Nervous System Tissue Sharon A. Louis and Brent A. Reynolds Isolation and Culture of Skeletal Muscle Myofibers as a Means to Analyze Satellite Cells Gabi Shefer and Zipora Yablonka-Reuveni Adult Ventricular Cardiomyocytes: Isolation and Culture Klaus-Dieter Schlьter and Daniela Schreiber Isolation and Culture of Primary Endothelial Cells Bruno Larrivйe and Aly Karsan Studying Leukocyte Rolling and Adhesion In Vitro Under Flow Conditions Susan L. Cuvelier and Kamala D. Patel Isolation and Characterization of Side Population Cells Margaret A. Goodell, Shannon McKinney-Freeman, and Fernando D. Camargo Scalable Production of Embryonic Stem Cell-Derived Cells Stephen M. Dang and Peter W. Zandstra Index
Автор: Cheryl D. Helgason; Cindy L. Miller Название: Basic Cell Culture Protocols ISBN: 1493956523 ISBN-13(EAN): 9781493956524 Издательство: Springer Рейтинг: Цена: 21661.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: At some point in their careers, virtually every scientist and technician, as well as many medical professionals, regardless of their area of specialization have a need to utilize cell culture systems. Updating and significantly expanding upon the previous editions, Basic Cell Culture Protocols, Fourth Edition provides the novice cell culturist with sufficient information to perform the basic techniques, to ensure the health and identity of their cell lines, and to be able to isolate and culture specialized primary cell types. The intent of this extensive volume is to generate a valuable resource containing clear methodologies pertinent to current areas of investigation, rather than attempting to educate cell culturists on specific cell types or organ systems. Written in the highly successful Methods in Molecular Biology™, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, Basic Cell Culture Protocols, Fourth Edition compiles the essential techniques needed to approach this vital laboratory activity with full success.
Автор: Scott H. Randell; M. Leslie Fulcher Название: Epithelial Cell Culture Protocols ISBN: 1493956795 ISBN-13(EAN): 9781493956791 Издательство: Springer Рейтинг: Цена: 20263.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: In its 2nd edition, this Methods in Molecular Biology (TM) volume provides a cross section of up-to-date culture protocols for the most studied cell systems and supporting technologies. Includes materials lists, step-by-step protocols and troubleshooting tips.
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