PCR in Bioanalysis, Stephen J Meltzer

Автор: Connell Joe
Название: RT-PCR Protocols
ISBN: 0896038750 ISBN-13(EAN): 9780896038752
Издательство: Springer
Цена: 13270.00 р.
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Описание: A panel of highly regarded molecular biologists and clinical researchers describe in detail their most novel, useful, and interesting RT-PCR applications. Here the newcomer will find readily reproducible protocols for highly sensitive detection and quantification of gene expression, the in situ localization of gene expression in tissue, and the cloning of genes, as well as for analyzing T-cell clones and the differential expression of genes. For the expert seeking to extend the usefulness of RT-PCR, there are user-friendly applications that complement the latest technological advances, including laser-capture microdissection (LCM), real-time and quantitative PCR, microarray technology, cDNA cloning, and antibody engineering.

Автор: Franck Pellestor
Название: PRINS and In Situ PCR Protocols
ISBN: 1617376361 ISBN-13(EAN): 9781617376368
Издательство: Springer
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Цена: 16769.00 р.
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Описание: Part I, Basic Methodology, contains chapters that provide useful protocols for many variations of PRINS and in situ PCR, including a new fast multicolor PRINS method, and protocols for PRINS detection of unique sequences in situ.

Автор: Sergio Colonna-Romano; Antonella Leone; Bruno Mare
Название: Differential-Display Reverse Transcription-PCR (DDRT-PCR)
ISBN: 3540632972 ISBN-13(EAN): 9783540632979
Издательство: Springer
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Цена: 18167.00 р.
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Описание: Identification of differentially expressed genes is one of the major challenges in molecular biology. This technique has been proven to be highly effective in identifying sequences that are differentially expressed in various cell types.

Автор: Y. M. Dennis Lo
Название: Clinical Applications of PCR
ISBN: 161737511X ISBN-13(EAN): 9781617375118
Издательство: Springer
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Цена: 21661.00 р.
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Описание: Highlights include real-time PCR, which allows the technique to be performed in a quantitative manner with improved sensitivity, robustness, and resilience to carryover contamination, mass spectrometric analysis of nucleic acids, and circulating cell-free nucleic acids in plasma.

Автор: Elizabeth van Pelt-Verkuil; Alex van Belkum; John
Название: Principles and Technical Aspects of PCR Amplification
ISBN: 9048175798 ISBN-13(EAN): 9789048175796
Издательство: Springer
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Цена: 18284.00 р.
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Описание: This publication aims to provide the reader with a guide to the standard PCR technique and its many available variants, with particular emphasis being placed on the role of these PCR techniques in the clinical diagnostic laboratory (the central theme of this book).

Автор: Yuryev Anton
Название: PCR Primer Design
ISBN: 158829725X ISBN-13(EAN): 9781588297259
Издательство: Springer
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Цена: 23757.00 р.
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Описание: At the heart of most high-throughput methods is the technique of polymerase chain reaction (PCR). With intricate descriptions of basic approaches as well as specialized methods, "PCR Primer Design" is an exceptional reference for all those involved in studying the genome.

Автор: Jiang Gu
Название: In Situ PCR and Related Technology
ISBN: 1468468456 ISBN-13(EAN): 9781468468458
Издательство: Springer
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Цена: 6986.00 р.
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Описание: Ever since the introduction of the polymerase chain reaction (peR) in 1986, morphologists, whose interests lie in the analysis of intact tissue structures, have been attempting to adapt this technique to intact cells or tissue sections to detect low copy numbers of DNA or RNA in situ while preserving tissue morphology.

Автор: Bing-Yuan Chen; Harry W. Janes
Название: PCR Cloning Protocols
ISBN: 1617372811 ISBN-13(EAN): 9781617372810
Издательство: Springer
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Цена: 25853.00 р.
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Описание: PCR Cloning Protocols, Second Edition, updates and expands Bruce White`s best-selling PCR Cloning Protocols (1997) with the newest procedures for DNA cloning and mutagenesis.

Автор: Ralph Rapley
Название: PCR Sequencing Protocols
ISBN: 1489940383 ISBN-13(EAN): 9781489940384
Издательство: Springer
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Цена: 16979.00 р.
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Описание: Here, as with enzymatic DNA sequencing, is a very simple concept that relies on minimal information to prepare short oligonucleotide primers that direct the synthesis of a specified fi-agment o f DNA in the presence of a thermostable DNA polymerase.