Автор: Poulopoulos Alexandros Название: Synapse Development: Methods and Protocols ISBN: 1493982737 ISBN-13(EAN): 9781493982738 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Contributed by experts and pioneers in their respective techniques, the book covers synapses in the brain and in culture, their constituents, their structures, their dynamics, and the assemblies they form, all in the structure of a laboratory guide.
Автор: Mishra Suresh Название: Immunometabolism: Methods and Protocols ISBN: 1071608045 ISBN-13(EAN): 9781071608043 Издательство: Springer Цена: 16769.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This detailed book showcases the tremendous effort and progress made in developing techniques and protocols for the study of immunometabolism, and in utilizing recent technological advances for probing and manipulating adipose and immune cells, and subsequently, their functions and immunometabolic consequences.
Автор: Jean Harry; Hugh A. Tilson Название: Neurodegeneration Methods and Protocols ISBN: 1617370983 ISBN-13(EAN): 9781617370984 Издательство: Springer Рейтинг: Цена: 20263.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Rapid advances in our understanding of basic cell biological processes and of the molecular mechanisms of cell function and dysfunction have led to an increasing interest in utilizing these approaches in neurobiological research.
Автор: Poss Kenneth D., Kьhn Bernhard Название: Cardiac Regeneration: Methods and Protocols ISBN: 1071606700 ISBN-13(EAN): 9781071606704 Издательство: Springer Рейтинг: Цена: 16769.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This detailed book addresses major goals of regenerative medicine and the cardiovascular research community with techniques to replenish lost cardiomyocytes, avoid scar-associated pathology, and improve myocardial infarction (MI) outcomes. The collection begins with a section on cardiac injury models, including zebrafish, neonatal and adult mice, and pigs, and continues with sections covering culturing cardiomyocytes from different species as well as methods for labeling or manipulation of cardiac tissue for the purpose of answering questions in regeneration. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cardiac Regeneration: Methods and Protocols provides the latest models and methods used in the field of heart regeneration, designed for researchers interested in establishing these assays in their laboratories to reproduce or extend findings, and for familiarizing themselves with the field if it is new to them. The chapter “Ventricular Cryoinjury as a Model to Study Heart Regeneration in Zebrafish” is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Автор: Fiedler Lorna R., Pellet-Many Caroline Название: VEGF Signaling: Methods and Protocols ISBN: 1071622161 ISBN-13(EAN): 9781071622162 Издательство: Springer Рейтинг: Цена: 21661.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume provides an updated collection of protocols for manipulating and studying VEGF signaling pathways in vitro and in vivo and aims to present a range of both firmly established and newly emerging technologies. Covering multiple model species, from mouse to zebrafish to human, the book explores the role of VEGF and VEGFR isoforms in exosomes, cultured cells, or in tissues, as well as robust cell assays for the investigation of basic angiogenic mechanisms and VEGF signaling in more complex cellular systems, amongst other subjects. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, VEGF Signaling: Methods and Protocols, Second Edition provides a useful tool for researchers in the vascular biology community and beyond in understanding the basic biology of VEGF signaling and in translating this research into the clinic.
Автор: Duque Paula Название: Environmental Responses in Plants: Methods and Protocols ISBN: 1493980297 ISBN-13(EAN): 9781493980291 Издательство: Springer Рейтинг: Цена: 16070.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
PaPart I Tropisms
1.11. Hydrotropism: Analysis of the Root Response to a Moisture Gradient Regina Antoni, Daniela Dietrich, Malcolm J. Bennett, and Pedro L. Rodriguez
2. Assessing Gravitropic Responses in Arabidopsis Richard Barker, Benjamin Cox, Logan Silber, Arah Sangari, Amir Assadi, and Patrick Masson
3. Physiological Analysis of Phototropic Responses in Arabidopsis Mathias Zeidler
4. Automatic Chloroplast Movement Analysis Henrik Johansson and Mathias Zeidler
5. Micro
scopic and Biochemical Visualization of Auxins in Plant Tissues Joshua J. Blakeslee and Angus S. Murphy
6. Immunolocalization of PIN and ABCB Transporters in Plants Nicola Carraro and Wendy A. Peer
Part II Photoperiodism and Circasian Rhythms
7.Analysis of Circadian Leaf Movements Niels A. Mьller and Josй M. Jimйmez-Gуmez
8. Sample Preparation of Arabidopsis Thaliana Shoot Apices for Expression Studies of Photoperiod-Induced Genes Fernando Andrйs, Stefano Torti, Coral Vincent, and George Coupland
9. A Luciferase-Based Assay to Test Whether Gene Expression Responses to Environmental Inputs are
Temporally Restricted by the Circadian Clock Amaury de Montaigu, Markus C. Berns, and Geroge Coupland
10. Identification of Arabidopsis Transcriptional Regulators by Yeast One-Hyrbid Screens Using a Transcription Factor ORFeome Ghislain Breton, Steve A. Kay, and Josй L. Pruneda-Paz
11. Monitoring Alternative Splicing Changes in Arabidopsis Circadian Clock Genes Craig G. Simpson, John Fuller, Cristiane P. G. Calixto, Jim McNicol, Clare Booth, John W. S. Brown, and Dorothee Staiger
12. Assessing the Impact of Photosynthetic Sugars in the Arabidopsis Circadian Clock Michael J. Haydon and Alex A. R. Webb
13. Assessing Protein Stability Under Different Light and Circadian Conditions Takatoshi Kiba and Rossana Henriques
Part IIIAbiotic Stress Responses
14. Screening for Abiotic Stress Tolerance in Rice: Salt, Cold, and Drought Diego M. Almeida, M. Cecнlia Almadanim, Tiago Louren
зo, Isabel A. Abreu, Nelson J. M. Saibo, and M. Margarida Oliveira
15. Basic Techniques to Assess Seed Germination Responses to Abiotic Stress in Arabidopsis Thaliana Urszula Piskurewicz and Luis Lopez-Molina
16. Assessing Tolerance to Heavy-Metal Stress in Arabidopsis Thaliana Seedings Estelle Remy and Paula Duque
17. Assessing Drought Responses Using Thermals Infrared Imaging Ankush Prashar and Hamlyn G. Jones
18. Generating Targeted Gene Knockout Lines in Physcomotrella Patens to Study Evolution of Stress- Responsive Mechanisms Monika Maronova and Maria Kalyna
19. Screening Stress Tolerance Traits in Arabidopsis Cell Cultures Imma P. Salamу, Bogбta Boros, and L
бszlу Szabados
20. Using Arabidopsis Protoplasts to Study Cellular responses to Environmental Stress Ana Confraria and Elena Baena-Gonzбlez
21. Construction of Artificial miRNAs to Prevent Drought Stress in Solanum Tuberosum Anna Wyrzykowska, Marcin Pieczyński, and Zofia Szweykowska-Kuli
Автор: Eyster Kathleen M. Название: Estrogen Receptors: Methods and Protocols ISBN: 1071619195 ISBN-13(EAN): 9781071619193 Издательство: Springer Рейтинг: Цена: 21661.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This fully updated collection includes chapters on a wide array of techniques that are vital for advancing our understanding of the physiological and pathological effects of estrogen, mediated by estrogen receptor ? (ER?) and estrogen receptor ? (ER?) as well as the non-genomic membrane-bound G-protein coupled estrogen receptor (GPER/GPR30). The protocols range from standard methods and important laboratory workhorses such as immunoblot and RT-qPCR, to newer technologies such as PET imaging, reporter animal models, polysome fractionation, and many others, involving a broad range of tissue types to demonstrate the variety of estrogen receptor effects. Written for this highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Estrogen Receptors: Methods and Protocols, Second Edition serves as an ideal guide to researchers working to explore the vibrancy and excitement of the field of estrogen receptors and estrogen actions.
Автор: Babetto Elisabetta Название: Axon Degeneration: Methods and Protocols ISBN: 1071605844 ISBN-13(EAN): 9781071605844 Издательство: Springer Цена: 23757.00 р. Наличие на складе: Поставка под заказ.
Описание: This book is a collection of classical as well as innovative methods used to investigate axon degeneration with a particular focus on addressing the common challenges encountered while performing these procedures.
Автор: Evangelou Evangelos, Veroniki Areti Angeliki Название: Meta-Research: Methods and Protocols ISBN: 1071615653 ISBN-13(EAN): 9781071615652 Издательство: Springer Рейтинг: Цена: 18167.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Chapters detail principles of systematic reviews, semi-automated tools for systematic searches, fixed- and random-effects meta-analytical models, living systematic reviews, meta-analysis of genetic studies, meta-analysis of pragmatic and explanatory trials, network meta-analysis, and other modern approaches for data synthesis.
Автор: Ramji Dipak Название: Atherosclerosis: Methods and Protocols ISBN: 1071619233 ISBN-13(EAN): 9781071619230 Издательство: Springer Рейтинг: Цена: 33401.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
PART I: Atherosclerosis: Pathogenesis, model systems and emerging concepts.
1. Atherosclerosis: Pathogenesis and key cellular processes, current and emerging therapies, key challenges and future research directions
Yee-Hung Chan and Dipak P. Ramji
2. Key roles of inflammation in atherosclerosis: Mediators involved in orchestrating the inflammatory response and its resolution in the disease along with therapeutic avenues targeting inflammation
Yee-Hung Chan and Dipak P. Ramji
3. Survey of in vitro model systems for investigation of key cellular processes associated with atherosclerosis
Dipak P. Ramji, Alaa Ismail, Jing Chen, Fahad Alradi, and Sulaiman Al Alawi
4. Survey of approaches for investigation of atherosclerosis in vivo
Dipak P. Ramji, Yee-Hung Chan, Alaa Alahmadi, Reem Alotibi, and Nouf Alshehri
5. Exploring the associations between clonal hematopoiesis of indeterminate potential, myeloid malignancy and atherosclerosis
Juan Bautista Menendez-Gonzalez, and Neil P. Rodrigues
6. Peptides as therapeutic agents for atherosclerosis
C. Roger White, Mayakonda Palgunachari, Paul Wolkowicz, and G. M. Anantharamaiah
PART II: In vitro and ex vivo model systems for atherosclerosis research.
7. Isolation and culturing of primary mouse and human macrophages
Alba de Juan and Begoсa Lavin
8. Effective transfection and gene silencing of primary murine macrophages with small interfering RNA
Kim van Kuijk, Jan Nagenborg, and Judith C. Sluimer
9. Monitoring celular proliferation, migration and apoptosis associated with atherosclerosis plaques in vitro
Kerry S. Wadey, Alexandros Somos, Stephen J. Cross, Lien M. Reolizo, Jason L. Johnson, and Sarah J. George
10. Monocyte adhesion assays for detecting endothelial cell activation in vascular inflammation and aterosclerosis
Keman Xu, Fatma Saaoud, Stephanie Yu, Charles Drummer IV, Ying Shao, Yu Sun, Yifan Lu, Jianxin Sun, Jun Yu, Xiaohua Jiang, Hong Wang, and Xiaofeng Yang
11. Chick embryo ex vivo aortic sprouting assays for cardiovascular research
Selvaraj Vimalraj and Anuradha Dhanasekaran
12. Purification and analysis of circulating lipid particles
Barnaby W. R. Roper, Basmah Al-Sayejh, Ahmed Al-Aufi, Gary A. Cuthbert, Katie Lacey, Shervanthi Homer-Vanniasinkam, Michael A. Harrison, Darren C. Tomlinson, Ramzi Ajjan, and Sreenivasan Ponnambalam
13. Preparation of LDL, oxidation, methods of detection and applications in atherosclerosis research
Chandrakala Aluganti Narasimhulu and Sampath Parthasarathy
14. Monitoring modified lipoprotein uptake and macropinocytosis associated with macrophage foam cell formation
Alaa A. Alahmadi and Dipak P. Ramji
15. A fluorescent based in vitro method to assess cholesterol efflux. Sara Fernбndez-Castillejo, Anna Pedret, Ъrsula Catalбn, and Rosa Solа
16. Monitoring the anisotropy and fluidity of the HDL monolayer as
surrogates of HDL functionality
Sara Fernбndez-Castillejo, Anna Pedret, Ъrsula Catalбn, Claude Motta, and Rosa Solа
17. Assessment of ex-vivo antioxidative potential of murine HDL in atherosclerosis
Andrea Rivas-Urbina, Noemi Rotllan, David Santos, Josep Julve, Joan Carles Escolа-Gil, and Jose Luis Sanchez-Quesada
18. Dual-fluorescence in vitro efferocytosis assay
Arif Yurdagul Jr
19. Investigation of mitochondrial bioenergetic profile and dysfunction in atherosclerosis
Yee-Hung Chan, and Dipak P. Ramji
20. P
Автор: Dahlmanns Jana, Dahlmanns Marc Название: Synaptic Vesicles: Methods and Protocols ISBN: 1071619152 ISBN-13(EAN): 9781071619155 Издательство: Springer Цена: 18167.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This book provides protocols on the preparation of synaptic vesicles from different model systems and provides instructions for functional investigations of their transport and formation, scaffolding, filling and priming, release, recycling, and degradation.
Автор: Magnus Lundgren Название: CRISPR : methods and protocols ISBN: 1493926861 ISBN-13(EAN): 9781493926862 Издательство: Springer Рейтинг: Цена: 23757.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume presents a list of cutting-edge protocols for the study of CRISPR-Cas defense systems and their applications at the genomic, genetic, biochemical and structural levels. CRISPR: Methods and Protocols guides readers through techniques that have been developed specifically for the analysis of CRISPR-Cas and techniques adapted from standard protocols of DNA, RNA and protein biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, CRISPR: Methods and Protocols provides a broad list of tools and techniques to study the interdisciplinary aspects of the prokaryotic CRISPR-Cas defense systems.
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