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CRISPR : methods and protocols, Magnus Lundgren


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Цена: 23757.00р.
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Автор: Magnus Lundgren
Название:  CRISPR : methods and protocols
ISBN: 9781493926862
Издательство: Springer
Классификация:



ISBN-10: 1493926861
Обложка/Формат: Hardback
Страницы: 366
Вес: 0.98 кг.
Дата издания: 20.05.2015
Серия: Methods in molecular biology
Язык: English
Издание: Softcover reprint of
Иллюстрации: 11 tables, black and white; 37 illustrations, color; 26 illustrations, black and white; xiv, 366 p. 63 illus., 37 illus. in color.
Размер: 188 x 264 x 21
Читательская аудитория: Professional & vocational
Подзаголовок: Methods and protocols
Ссылка на Издательство: Link
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Поставляется из: Германии
Описание: This volume presents a list of cutting-edge protocols for the study of CRISPR-Cas defense systems and their applications at the genomic, genetic, biochemical and structural levels. CRISPR: Methods and Protocols guides readers through techniques that have been developed specifically for the analysis of CRISPR-Cas and techniques adapted from standard protocols of DNA, RNA and protein biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, CRISPR: Methods and Protocols provides a broad list of tools and techniques to study the interdisciplinary aspects of the prokaryotic CRISPR-Cas defense systems.
Дополнительное описание: Investigating CRISPR RNA Biogenesis and Function using RNA-seq.- In vitro Co-reconstitution of Cas Protein Complexes.- Analysis of CRISPR pre-crRNA Cleavage.- Annotation and Classification of CRISPR-Cas Systems.- Computational Detection of CRISPR/crRNA Ta



Affinity Chromatography / Methods and Protocols

Автор: Zachariou Michael
Название: Affinity Chromatography / Methods and Protocols
ISBN: 1588296598 ISBN-13(EAN): 9781588296597
Издательство: Springer
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Цена: 2000.00 р.
Наличие на складе: Есть (2 шт.)
Описание: Thirty-eight years after its introduction, affinity chromatography remains a key tool in th armory of separation techniques available to separation and interaction scientists.  Expanded and updated from the first edition, this second edition volume aims to provide the beginner with the practical knowledge needed to develop affinity separations suitable for a variety of applications relevant to the post-genomic era.

Single Nucleotide Polymorphisms / Methods and Protocols

Автор: Kwok Pui-Yan
Название: Single Nucleotide Polymorphisms / Methods and Protocols
ISBN: 0896039684 ISBN-13(EAN): 9780896039681
Издательство: Springer
Цена: 5809.00 р.
Наличие на складе: Есть (2 шт.)
Описание: Pui-Yan Kwok, MD, PhD, has assembled a comprehensive collection of readily reproducible techniques for the difficult process of single nucleotide polymorphisms (SNP) discovery and genotyping. These cutting-edge protocols for mutation/SNP detection utilize denaturing high-performance liquid chromatography (dHPLC), single-strand conformation polymorphism (SSCP), conformation-sensitive gel electrophoresis (CSGE), chemical cleavage, and direct sequencing. Equally powerful and up-to-date methods are given for genotyping SNPs, including molecular beacons, the Taqman assay, single-base extension approaches, pyrosequencing, ligation, the Invader assay, and primer extension with mass spectrometry detection.

Autoimmunity: Methods and Protocols (Methods in Molecular Biology)

Автор: Andras Perl (Editor)
Название: Autoimmunity: Methods and Protocols (Methods in Molecular Biology)
ISBN: 1607617196 ISBN-13(EAN): 9781607617198
Издательство: Springer
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Цена: 20263.00 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание: Continued refinement of wide-spread access to transgenic technology has allowed for new animal models have been developed that exhibit features of autoimmune disease have been developed that exhibit features of autoimmune disease.

Neuronal Cell Culture: Methods and Protocols (2013) ( Methods in Molecular Biology #1078 )

Автор: Amini, Shohreh (Editor), White, Martyn K (Editor)
Название: Neuronal Cell Culture: Methods and Protocols (2013) ( Methods in Molecular Biology #1078 )
ISBN: 1627036393 ISBN-13(EAN): 9781627036399
Издательство: Springer
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Цена: 20962.00 р.
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Описание: This book details the latest aspects of the culture of neural cells and explains the practical and theoretical considerations of the techniques involved. It features step-by-step, readily reproducible laboratory protocols and expert implementation advice.

Nucleic Acid Detection: Methods and Protocols (Methods in Molecular Biology)

Автор: Dmitry M. Kolpashchikov (Editor), Yulia V. Gerasim
Название: Nucleic Acid Detection: Methods and Protocols (Methods in Molecular Biology)
ISBN: 1627035346 ISBN-13(EAN): 9781627035347
Издательство: Springer
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Цена: 16769.00 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание: This Methods in Molecular Biology book details approaches for the detection of DNA and RNA including recovery of trace amounts of DNA for amplification and analysis, new qPCR chemistries, step-by-step, reproducible protocols, materials lists, tips and more.

Virus-Host Interactions: Methods and Protocols (Methods in Molecular Biology)

Автор: Susanne M. Bailer (Editor), Diana Lieber (Editor)
Название: Virus-Host Interactions: Methods and Protocols (Methods in Molecular Biology)
ISBN: 1627036008 ISBN-13(EAN): 9781627036009
Издательство: Springer
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Цена: 23757.00 р.
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Описание: This volume covers various aspects of virological research, including molecular interactions and regulatory mechanisms on the protein as well as the RNA level. It features step-by-step, readily reproducible protocols and key implementation advice.

Neutrophil Methods and Protocols

Автор: Quinn
Название: Neutrophil Methods and Protocols
ISBN: 1627038442 ISBN-13(EAN): 9781627038447
Издательство: Springer
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Цена: 30745.00 р.
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Описание: procedures for subcellular fractionation of human neutrophils, analysis of in vivo transmigrated neutrophils, generation of mature neutrophils from induced pluripotent stem cells and analysis of neutrophil gene expression;

CRISPR-Cas Systems

Автор: Rodolphe Barrangou; John van der Oost
Название: CRISPR-Cas Systems
ISBN: 3642346561 ISBN-13(EAN): 9783642346569
Издательство: Springer
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Цена: 26122.00 р.
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Описание: This book surveys research on a wide range of newly identified CRISPR-Cas systems, which defend bacteria and archaea against viruses and plasmids. Includes structural and functional analyses offering new insight into their functions and possible applications.


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