Описание: 1. History of Electrophysiology and the Patch-Clamp
Alexej Verkhratysky and Vladimir Parpura
Part I. Pharmacology
2. Whole-Cell Patch-Clamp Analysis of Recombinant NMDA Receptor Pharmacology Using Brief Glutamate Applications
Nate Glasgow and Jon W. Johnson
3. Patch-Clamp Combined with Voltage/Concentration Clamp to Determine the Kinetics and Voltage Dependency of N-methyl-D-aspartate (NMDA) Receptor Open Channel Blockers
Chris G. Parsons and Kate E. Gilling
4. State-of-the-art Automated Patch-Clamp: Heat Activation, Action Potentials, and High Throughput in Ion Channel Screening
Sonja Stoelzle-Feix
5. Industrializing Electrophysiology: HT Automated Patch-Clamp on SyncroPatch(R) 96 Using Instant Frozen Cells
Liudmila Polonchuk
6. Planar Patch Clamp for Neuronal Networks-Considerations and Future Perspectives
Alessandro Bosca, Marzia Martina, and Christophe Py
Part II. Physiology
7. Methods for the Study of Synaptic Receptor Functional Properties
Enrica Maria Petrini and Andrea Barberis
8. Single-Cell RT-PCR, A Technique to Decipher the Electrical, Anatomical and Genetic Determinants of Neuronal Diversity
Maria Toledo-Rodriguez and Henry Markram
9. Whole-Cell Voltage-Clamp on Skeletal Muscle Fibers with the Silicone-Clamp Technique
Romain Lefebvre, Sandrine Pouvreau, Claude Collet, B
runo Allard, and Vincent Jacquemond
10. Multiphoton Imaging Approaches for Studying Striatal Dendritic Excitability
Joshua L. Plotkin and James D. Surmeier
11. Investigation of Synaptic Microcircuits Using Paired Patch-Clamp Recordings in Acute Brain Slices
Giovanni Russo and Stefano Taverna
12. Outside-Out 'Sniffer-patch' Clamp Technique for in situ Measures of Neurotransmitter Release
Йmilie Muller-Chrйtien
13. A Cost Effective Method for Preparing, Maintaining and Transfecting Neurons in Organotypic Slices
Cary Soares, Kevin F.H. Lee, Denise Cook, and Jean-Claude Bйпque
14. Acute Brain Slice Methods for Adult and Aging Animals: Application of Targeted Patch Clamp Analysis and Optogenetics
Jonathan T. Ting, Tanya L. Daigle, Qian Chen, and Guoping Feng
15. Synaptic Connectivity in Engineered Neuronal Networks
Peter Molnar, Jung-Fong Kang, Neelima Bhargava, Mainak Das, and James J. Hickman
16. Modeling of Action Potential Generation in NG108-15 Cells
Peter Molnar and James J. Hickman
17. Whole-Cell Patch-Clamp Recordings in Freely Moving Animals
Albert K. Lee, Jйrфme Epsztein, and Michael Brecht
Part III. Biophysics
18. Pressure-Polished Borosilicate Pipettes are "Universal Sealer" Yielding Low Access Resistance and Efficient Intracellular Perfusion
Marco Aquila, Mascia B
enedusi, Anna Fasoli, and Giorgio Rispoli
19. Current Recordings at the Single Channel Level in Adult Mammalian Isolated Cardiomyocytes
Romain Guinamard, Thomas Hof, and Laurent Sallй II
20. Dynamic Clamp as a Tool to Study the Functional Effects of Individual Membrane Currents
Gйza Berecki, Arie O. Verkerk, Antoni C.G. van Ginneken, and Ronald Wilders
21. Dynamic Clamp in Cardiac and Neuronal Systems Using RTXI
Francis A. Ortega, Robert J. Butera, David J. Christini, John A. White, and Alan D. Dorval
22. Ion Selectivity of Pore-Forming Peptides and Ion Channels Measured in Xenopus Oocytes
Thierry Cens and Pierre Charnet
23. Principles of Single-Channel Kinetic Analysis
Feng Qin
