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Metabolic Profiling: Methods and Protocols, Theodoridis Georgios A., Gika Helen G., Wilson Ian D.


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Автор: Theodoridis Georgios A., Gika Helen G., Wilson Ian D.
Название:  Metabolic Profiling: Methods and Protocols
ISBN: 9781493976423
Издательство: Springer
Классификация:
ISBN-10: 1493976427
Обложка/Формат: Hardcover
Страницы: 291
Вес: 0.74 кг.
Дата издания: 16.04.2018
Серия: Methods in molecular biology
Язык: English
Издание: 1st ed. 2019
Иллюстрации: 28 tables, color; 28 illustrations, color; 8 illustrations, black and white; viii, 252 p. 36 illus., 28 illus. in color.
Размер: 254 x 178 x 18
Читательская аудитория: Postgraduate, research & scholarly
Подзаголовок: Methods and protocols
Ссылка на Издательство: Link
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Поставляется из: Германии
Описание:

Preface...

Table of Contents...

Contributing Authors...


Part I Fundamentals

1. Metabolic Profiling: Topic, Challenges, Perspective

Helen G. Gika, Georgios A. Theodoridis, and Ian D. Wilson


2. Quality Control and Validation Issues in LC-MS Metabolomics

Olga Begou, Helen G. Gika, Georgios A. Theodoridis, and Ian D. Wilson


3. Data Treatment for LC-MS Untargeted Analysis

Samantha Riccadonna and Pietro Franceschi


4. Bio- and Chemoinformatics Approaches for Metabolomics Data Analysis

Michael Witting


Part II Methods

5. HILIC- MS/MS Multi-Targeted Method for Metabolomics Applications

Christina Virgiliou, Helen G. Gika, and Georgios A. Theodoridis


6. Ion Pair Chromatography for Endogenous Metabolites LC-MS Analysis in Tissue Samples Following Targeted Acquisition

Filippos Michopoulos


7. LC-MS Untargeted Analysis

Elizabeth Want


8. NMR Based Metabolic Profiling Procedures for Biofluids, and Cell and Tissue Extracts

Dimitra Benaki and Emmanuel Mikros


9. Untargeted GC-MS Metabolomics

Μatthaios-Εmmanouil P. Papadimitropoulos, Catherine G. Vasilopoulou, Christoniki Maga-Nteve, and Maria I. Klapa


10. Rat Fecal Metabolomics Based Analysis

Olga Deda, Helen G. Gika, and Georgios A. Theodoridis


11. GC-MS Metabolomic Profiling of Protic Metabolites Following Heptafluorobutyl Chloroformate Mediated Dispersive Liquid Microextraction Sample Preparation Protocol

Petr Husek, Zdeněk Svagera, Dagmar Hanzlнkovб, Iva Opekarovб, Lucie Řimnбčovб, Helena Zahradnнčkovб, and Petr Simek


12. Sheathless Capillary Electrophoresis-Mass Spectrometry for the Profiling of Charged Metabolites in Biological Samples

Rawi Ramautar


Part III Plant/Food and Other Applications

13. Two-Phase Extraction for Comprehensive Analysis of the Plant Metabolome by NMR

Jan Schripsema and Denise Dagnino


14. NMR Spectroscopy Protocols for Food Metabolomics Applications

Evangelia Ralli, Maria Amargianitaki, Efi Manolopoulou, Maria Misiak, Georgios Markakis, Sofia Tachtalidou, Alexandra Kolesnikova, Photis Dais, and Apostolos Spyros


15. Direct Injection Analysis of Fruit VOCs by PTR-ToF-MS: The Apple Case Study

Brian Farneti


16. LC-MS Untargeted Protocol for the Analysis of Wine

Panagiotis Arapitsas and Fulvio Mattivi


Part IV Life Science Applications

17. Tissue Multiplatform-Based Metabolomics/Metabonomics for Enhanced Metabolome Coverage

Panagiotis A. Vorkas, M.R. Abellona U, and Jia V. Li


18. UHPLC-HRMS Analysis for Steroid Profiling in Serum (Steroidomics)

Federico Ponzetto, Julien Boccard, Raul Nicoli, Tiia Kuuranne, Martial Saugy, and Serge Rudaz


19. Metabolomics in Human Acute-Exercise Trials: Study Design and Preparation

Aikaterina Siopi and Vassilis Mougios





Proteomic Profiling and Analytical Chemistry

Автор: Pawel Ciborowski
Название: Proteomic Profiling and Analytical Chemistry
ISBN: 0444636889 ISBN-13(EAN): 9780444636881
Издательство: Elsevier Science
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Цена: 17517.00 р.
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Описание:

"Proteomic Profiling and Analytical Chemistry: The Crossroads, Second Edition" helps scientists without a strong background in analytical chemistry to understand principles of the multistep proteomic experiment necessary for its successful completion. It also helps researchers who do have an analytical chemistry background to break into the proteomics field. Highlighting points of junction between proteomics and analytical chemistry, this resource links experimental design with analytical measurements, data analysis, and quality control. This targeted point of view will help both biologists and chemists to better understand all components of a complex proteomic study.

The book provides detailed coverage of experimental aspects such as sample preparation, protein extraction and precipitation, gel electrophoresis, microarrays, dynamics of fluorescent dyes, and more. The key feature of this book is a direct link between multistep proteomic strategy and quality control routinely applied in analytical chemistry. This second edition features a new chapter on SWATH-MS, substantial updates to all chapters, including proteomic database search and analytical quantification, expanded discussion of post-hoc statistical tests, and additional content on validation in proteomics.
Covers the analytical consequences of protein and peptide modifications that may have a profound effect on how and what researchers actually measureIncludes practical examples illustrating the importance of problems in quantitation and validation of biomarkersHelps in designing and executing proteomic experiments with sound analytics

Immunocytochemical methods and protocols

Автор: Constance Oliver; Maria Celia Jamur (Eds.)
Название: Immunocytochemical methods and protocols
ISBN: 1588294633 ISBN-13(EAN): 9781588294630
Издательство: Springer
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Цена: 9084.00 р.
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Описание: This third edition reflects the rapid evolution of immunocytochemistry in the post-genomic era. Expert researchers explore the latest methods, now widely used in situ to identify components of cells and tissues in both normal and pathological conditions.

Modeling Peptide-Protein Interactions : Methods and Protocols

Автор: Schueler-Furman, Ora;London, Nir
Название: Modeling Peptide-Protein Interactions : Methods and Protocols
ISBN: 1493967967 ISBN-13(EAN): 9781493967964
Издательство: Springer
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Цена: 13974.00 р.
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Описание: This volume covers an array of techniques available for studying peptide-protein docking and design.

Metabolic Profiling

Автор: Thomas O. Metz
Название: Metabolic Profiling
ISBN: 149395816X ISBN-13(EAN): 9781493958160
Издательство: Springer
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Цена: 20263.00 р.
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Описание: This book presents protocols that are illustrative of the evolution of metabolic profiling from single molecule analysis to global metabolome profiling. It features cutting-edge protocols, presented from the perspective of leading experts in the field.

Metabolic Profiling: Disease and Xenobiotics

Название: Metabolic Profiling: Disease and Xenobiotics
ISBN: 1849731632 ISBN-13(EAN): 9781849731638
Издательство: Royal Society of Chemistry
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Цена: 36960.00 р.
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Описание: This exemplary new book highlights applications of multivariate techniques in the areas of drug therapy and toxicology, cancer, obesity and diabetes, as well as outlining applications to cardiovascular, infectious, inflammatory and oral diseases in detail.

High-Resolution Profiling of Protein-RNA Interactions

Автор: Mathias Munschauer
Название: High-Resolution Profiling of Protein-RNA Interactions
ISBN: 3319162527 ISBN-13(EAN): 9783319162522
Издательство: Springer
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Цена: 18167.00 р.
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Описание: The work reported in this book represents an excellent example of how creative experimentation and technology development, complemented by computational data analysis, can yield important insights that further our understanding of biological entities from a systems perspective. The book describes how the study of a single RNA-binding protein and its interaction sites led to the development of the novel ‘protein occupancy profiling’ technology that for the first time captured the mRNA sequence space contacted by the ensemble of expressed RNA binders. Application of protein occupancy profiling to eukaryotic cells revealed that extensive sequence stretches in 3’ UTRs can be contacted by RBPs and that evolutionary conservation as well as negative selection act on protein-RNA contact sites, suggesting functional importance. Comparative analysis of the RBP-bound sequence space has the potential to unravel putative cis-acting RNA elements without a priori knowledge of the bound regulators. Here, Dr. Munschauer provides a comprehensive introduction to the field of post-transcriptional gene regulation, examines state-of-the-art technologies, and combines the conclusions from several journal articles into a coherent and logical story from the frontiers of systems-biology inspired life science. This thesis, submitted to the Department of Biology, Chemistry and Pharmacy at Freie Universit?t Berlin, was selected as outstanding work by the Berlin Institute for Medical Systems Biology at the Max-Delbrueck Center for Molecular Medicine, Germany.

Protein Terminal Profiling

Автор: Oliver Schilling
Название: Protein Terminal Profiling
ISBN: 1493968491 ISBN-13(EAN): 9781493968497
Издательство: Springer
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Цена: 13974.00 р.
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Описание:

Preface...
Table of Contents...
Contributing Authors...

1. [14C]-Acetyl-CoenzymeA-Based In Vitro N-Terminal Acetylation Assay
Adrian Drazic and Thomas Arnesen

2. DTNB-Based Quantification of In Vitro Enzymatic N-Terminal Acetyltransferase Activity
Hеvard Foyn, Paul R. Thompson, and Thomas Arnesen

3. SILProNAQ: A Convenient Approach for Proteome-Wide Analysis of Protein N-Termini and N-Terminal Acetylation Quantitation
Willy V. Bienvenut, Carmela Giglione, and Thierry Meinnel

4. Profiling of Protein N-Termini and their Modifications in Complex Samples
Fatih Demir, Stefan Niedermaier, Jayachandran N. Kizhakkedathu, and Pitter F. Huesgen

5. Protease Substrate Profiling by N-Terminal COFRADIC
An Staes, Petra Van Damme, Evy Timmerman, Bart Ruttens, Elisabeth Stes, Kris Gevaert, and Francis Impens

6. Doublet N-Terminal Oriented Proteomics for N-Terminomics and Proteolytic Processing Identification
Benoit Westermann, Alvaro Sebastian Vaca Jacome, Magali Rompais, Christine Carapito, and Christine Schaeffer-Reiss

7. Multidimensional Analysis of Protease Substrates and their Cellular Origins in Mixed Secretomes from Multiple Cell Types
Pascal Schlage and Ulrich auf dem Keller

8. System-Wide Profiling of Protein Amino Termini from Formalin-Fixed, Paraffin-Embedded Tissue Specimens for the Identification of Novel Substrates
Zon W. Lai and Oliver Schilling

9. Identification of Carboxypeptidase Substrates by C-Terminal COFRADIC
Sebastian Tanco, Francesc Xavier Aviles, Kris Gevaert, Julia Lorenzo, and Petra Van Damme

10. ProC-TEL: Profiling of Protein C-Termini by Enzymatic Labeling
Wenwen Duan and Guoqiang Xu

11. Determining Protease Substrates within a Complex Protein Background using the PROtein TOpography and Migration Analysis Platform (PROTOMAP)
R.A. Fuhrman-Luck, L.M. Silva, M.L. Hastie, J.J. Gorman, J.A. Clements

12. Multiplexed Protease Specificity Profiling Using Isobaric Labeling
Joanna Tucher and Andreas Tholey

13. FPPS - Fast Profiling of Protease Specificity
Matej Vizovisek, Robert Vidmar, and Marko Fonovic

14. Profiling of Protease Cleavage Sites by Proteome-Derived Peptide Libraries and Quantitative Proteomics
Chia-yi Chen, Bettina Mayer, and Oliver Schilling

15. Prediction of Proteases Involved in Peptide Generation
Mercedes Arguello Casteleiro, Robert Stevens, and Julie Klein

16. Live-Cell Imaging of Protease Activity: Assays to Screen Therapeutic Approaches
Anita Chalasani, Kyungmin Ji, Mansoureh Sameni, Samia H. Mazumder, Yong Xu, Kamiar Moin, and Bonnie F. Sloane

17. Protein Translocation Assays to Probe Protease Function and Screen for Inhibitors
Angelina Hahlbrock, Dorothйe Gц wein, and Roland H. Stauber

18. Simultaneous Detection of Metalloprotease Activities in Complex Biological Samples Using the PrAMA (Proteolytic Activity Matrix Assay) Method
Catharina Conrad, Miles A. Miller, Jцrg W Bartsch, Uwe Schlomann, Douglas A. Lauffenburger

19. Synthesis and Application of Activity-Based Probes for Proteases
Tim Van Kersavond, Minh T. N. Nguyen, and Steven H. L. Verhelst

Kinase Screening and Profiling

Автор: Hicham Zegzouti; Said A. Goueli
Название: Kinase Screening and Profiling
ISBN: 1493949233 ISBN-13(EAN): 9781493949236
Издательство: Springer
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Цена: 13275.00 р.
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Описание:

1. HTRF Kinase Assay Development and Methods in Inhibitor Characterization
Yong Jia, Mari Manuia, and Jose Juarez

2. Application of Eukaryotic Elongation Factor-2 Kinase (eEF-2K) for Cancer Therapy: Expression, Purification, and High-Throughput Inhibitor Screening
Clint D. J. Tavares, Ashwini K. Devkota, Kevin N. Dalby, and Eun Jeong Cho

3. Recombinant Kinase Production and Fragment Screening by NMR Spectroscopy
Byeonggu Han and Hee-Chul Ahn

4. Bioluminescence Methods for Assaying Kinases in Quantitative High-Throughput Screening (qHTS) Format Applied to Yes1 Tyrosine Kinase, Glucokinase and PI5P4Kα Lipid Kinase
Mindy I. Davis, Douglas S. Auld, and James Inglese

5. Using Bioluminescent Kinase Profiling Strips to Identify Kinase Inhibitor Selectivity and Promiscuity
Hicham Zegzouti, Jacquelyn Hennek, and Said A. Goueli

6. Measuring Activity of Phosphoinositide Lipid Kinases Using a Bioluminescent ADP-Detecting Assay
Andrew W. Tai and Jolanta Vidugiriene

7. A High-Throughput Radiometric Kinase Assay
Krisna C. Duong-Ly and Jeffrey R. Peterson

8. A High Content Assay to Screen for Modulators of EGFR Function
Christophe Antczak and Hakim Djaballah
9. Monitoring Protein Kinase Expression and Phosphorylation in Cell Lysates with Antibody Microarrays
Hong Zhang, Xiaoqing Shi, and Steven Pelech

10. From Enzyme to Whole Blood: Sequential Screening Procedure for Identification and Evaluation of p38 MAPK Inhibitors
Silke M. Bauer, Jakub M. Kubiak, Ulrich Rothbauer, and Stefan Laufer

11. Genetically Encoded Fluorescent Indicators to Visualize Protein Phosphorylation in Living Cells
Moritoshi Sato and Yoshio Umezawa

12. Characterization of an Engineered Src Kinase to Study Src Signaling and Biology
Leanna R. Gentry, Andrei V. Karginov, Klaus M. Hahn, and Channing J. Der

13. Screening One-Bead-One-Compound Peptide Libraries for Optimal Kinase Substrates
Thi B. Trinh and Dehua Pei

14. Determination of the Substrate Specificity of Protein Kinases with Peptide Micro- and Macroarrays
Shenshen Lai, Dirk F.H. Winkler, Hong Zhang, and Steven Pelech

15. Rapid Identification of Protein Kinase Phosphorylation Site Motifs Using Combinatorial Peptide Libraries
Chad J. Miller and Benjamin E. Turk

Proteomic Profiling

Автор: Anton Posch
Название: Proteomic Profiling
ISBN: 1493925490 ISBN-13(EAN): 9781493925490
Издательство: Springer
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Цена: 30745.00 р.
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Описание: This volume presents the latest developments of the main pillars of protein analysis, such as sample preparation, separation and characterization. The book begins by describing basic but important sample preparation protocols. It then goes on to describe more sophisticated procedures on enriching specific protein classes and concludes with detailed descriptions of integrated work-flows for comprehensive protein analysis and characterization. The authors of the individual chapters are renowned protein biochemists who have all set value to provide a detailed representation of their lab work. Throughout the chapters, these authors share important tips and tricks for a successful and reproducible employment of their protocols in other laboratories. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Proteomic Profiling: Methods and Protocols is the perfect guide for students of Biochemistry, Biomedicine, Biology, and Genomics and will be an invaluable source for the experienced, practicing scientists.

Activity-Based Protein Profiling

Автор: Stephan A. Sieber
Название: Activity-Based Protein Profiling
ISBN: 3642448313 ISBN-13(EAN): 9783642448317
Издательство: Springer
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Цена: 36570.00 р.
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Описание: SieberActivity-Based Protein Profiling for Natural Product Target Discovery, by Joanna Krysiak und Rolf BreinbauerPhotoaffinity Labeling in Activity-Based Protein Profiling, by Paul P. TateFunctional Analysis of Protein Targets by Metabolomic Approaches, by Yun-Gon Kim und Alan Saghatelian


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