Metabolic Profiling: Methods and Protocols, Theodoridis Georgios A., Gika Helen G., Wilson Ian D.
Автор: Pawel Ciborowski Название: Proteomic Profiling and Analytical Chemistry ISBN: 0444636889 ISBN-13(EAN): 9780444636881 Издательство: Elsevier Science Рейтинг: Цена: 17517.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
"Proteomic Profiling and Analytical Chemistry: The Crossroads, Second Edition" helps scientists without a strong background in analytical chemistry to understand principles of the multistep proteomic experiment necessary for its successful completion. It also helps researchers who do have an analytical chemistry background to break into the proteomics field. Highlighting points of junction between proteomics and analytical chemistry, this resource links experimental design with analytical measurements, data analysis, and quality control. This targeted point of view will help both biologists and chemists to better understand all components of a complex proteomic study.
The book provides detailed coverage of experimental aspects such as sample preparation, protein extraction and precipitation, gel electrophoresis, microarrays, dynamics of fluorescent dyes, and more. The key feature of this book is a direct link between multistep proteomic strategy and quality control routinely applied in analytical chemistry. This second edition features a new chapter on SWATH-MS, substantial updates to all chapters, including proteomic database search and analytical quantification, expanded discussion of post-hoc statistical tests, and additional content on validation in proteomics. Covers the analytical consequences of protein and peptide modifications that may have a profound effect on how and what researchers actually measureIncludes practical examples illustrating the importance of problems in quantitation and validation of biomarkersHelps in designing and executing proteomic experiments with sound analytics
Автор: Constance Oliver; Maria Celia Jamur (Eds.) Название: Immunocytochemical methods and protocols ISBN: 1588294633 ISBN-13(EAN): 9781588294630 Издательство: Springer Рейтинг: Цена: 9084.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This third edition reflects the rapid evolution of immunocytochemistry in the post-genomic era. Expert researchers explore the latest methods, now widely used in situ to identify components of cells and tissues in both normal and pathological conditions.
Автор: Schueler-Furman, Ora;London, Nir Название: Modeling Peptide-Protein Interactions : Methods and Protocols ISBN: 1493967967 ISBN-13(EAN): 9781493967964 Издательство: Springer Рейтинг: Цена: 13974.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume covers an array of techniques available for studying peptide-protein docking and design.
Автор: Thomas O. Metz Название: Metabolic Profiling ISBN: 149395816X ISBN-13(EAN): 9781493958160 Издательство: Springer Рейтинг: Цена: 20263.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This book presents protocols that are illustrative of the evolution of metabolic profiling from single molecule analysis to global metabolome profiling. It features cutting-edge protocols, presented from the perspective of leading experts in the field.
Название: Metabolic Profiling: Disease and Xenobiotics ISBN: 1849731632 ISBN-13(EAN): 9781849731638 Издательство: Royal Society of Chemistry Рейтинг: Цена: 36960.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This exemplary new book highlights applications of multivariate techniques in the areas of drug therapy and toxicology, cancer, obesity and diabetes, as well as outlining applications to cardiovascular, infectious, inflammatory and oral diseases in detail.
Автор: Mathias Munschauer Название: High-Resolution Profiling of Protein-RNA Interactions ISBN: 3319162527 ISBN-13(EAN): 9783319162522 Издательство: Springer Рейтинг: Цена: 18167.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: The work reported in this book represents an excellent example of how creative experimentation and technology development, complemented by computational data analysis, can yield important insights that further our understanding of biological entities from a systems perspective. The book describes how the study of a single RNA-binding protein and its interaction sites led to the development of the novel ‘protein occupancy profiling’ technology that for the first time captured the mRNA sequence space contacted by the ensemble of expressed RNA binders. Application of protein occupancy profiling to eukaryotic cells revealed that extensive sequence stretches in 3’ UTRs can be contacted by RBPs and that evolutionary conservation as well as negative selection act on protein-RNA contact sites, suggesting functional importance. Comparative analysis of the RBP-bound sequence space has the potential to unravel putative cis-acting RNA elements without a priori knowledge of the bound regulators. Here, Dr. Munschauer provides a comprehensive introduction to the field of post-transcriptional gene regulation, examines state-of-the-art technologies, and combines the conclusions from several journal articles into a coherent and logical story from the frontiers of systems-biology inspired life science. This thesis, submitted to the Department of Biology, Chemistry and Pharmacy at Freie Universit?t Berlin, was selected as outstanding work by the Berlin Institute for Medical Systems Biology at the Max-Delbrueck Center for Molecular Medicine, Germany.
Автор: Oliver Schilling Название: Protein Terminal Profiling ISBN: 1493968491 ISBN-13(EAN): 9781493968497 Издательство: Springer Рейтинг: Цена: 13974.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
Preface... Table of Contents... Contributing Authors...
1. [14C]-Acetyl-CoenzymeA-Based In Vitro N-Terminal Acetylation Assay Adrian Drazic and Thomas Arnesen 2. DTNB-Based Quantification of In Vitro Enzymatic N-Terminal Acetyltransferase Activity Hеvard Foyn, Paul R. Thompson, and Thomas Arnesen 3. SILProNAQ: A Convenient Approach for Proteome-Wide Analysis of Protein N-Termini and N-Terminal Acetylation Quantitation Willy V. Bienvenut, Carmela Giglione, and Thierry Meinnel 4. Profiling of Protein N-Termini and their Modifications in Complex Samples Fatih Demir, Stefan Niedermaier, Jayachandran N. Kizhakkedathu, and Pitter F. Huesgen 5. Protease Substrate Profiling by N-Terminal COFRADIC An Staes, Petra Van Damme, Evy Timmerman, Bart Ruttens, Elisabeth Stes, Kris Gevaert, and Francis Impens 6. Doublet N-Terminal Oriented Proteomics for N-Terminomics and Proteolytic Processing Identification Benoit Westermann, Alvaro Sebastian Vaca Jacome, Magali Rompais, Christine Carapito, and Christine Schaeffer-Reiss 7. Multidimensional Analysis of Protease Substrates and their Cellular Origins in Mixed Secretomes from Multiple Cell Types Pascal Schlage and Ulrich auf dem Keller 8. System-Wide Profiling of Protein Amino Termini from Formalin-Fixed, Paraffin-Embedded Tissue Specimens for the Identification of Novel Substrates Zon W. Lai and Oliver Schilling 9. Identification of Carboxypeptidase Substrates by C-Terminal COFRADIC Sebastian Tanco, Francesc Xavier Aviles, Kris Gevaert, Julia Lorenzo, and Petra Van Damme 10. ProC-TEL: Profiling of Protein C-Termini by Enzymatic Labeling Wenwen Duan and Guoqiang Xu 11. Determining Protease Substrates within a Complex Protein Background using the PROtein TOpography and Migration Analysis Platform (PROTOMAP) R.A. Fuhrman-Luck, L.M. Silva, M.L. Hastie, J.J. Gorman, J.A. Clements 12. Multiplexed Protease Specificity Profiling Using Isobaric Labeling Joanna Tucher and Andreas Tholey 13. FPPS - Fast Profiling of Protease Specificity Matej Vizovisek, Robert Vidmar, and Marko Fonovic 14. Profiling of Protease Cleavage Sites by Proteome-Derived Peptide Libraries and Quantitative Proteomics Chia-yi Chen, Bettina Mayer, and Oliver Schilling 15. Prediction of Proteases Involved in Peptide Generation Mercedes Arguello Casteleiro, Robert Stevens, and Julie Klein 16. Live-Cell Imaging of Protease Activity: Assays to Screen Therapeutic Approaches Anita Chalasani, Kyungmin Ji, Mansoureh Sameni, Samia H. Mazumder, Yong Xu, Kamiar Moin, and Bonnie F. Sloane 17. Protein Translocation Assays to Probe Protease Function and Screen for Inhibitors Angelina Hahlbrock, Dorothйe Gц wein, and Roland H. Stauber 18. Simultaneous Detection of Metalloprotease Activities in Complex Biological Samples Using the PrAMA (Proteolytic Activity Matrix Assay) Method Catharina Conrad, Miles A. Miller, Jцrg W Bartsch, Uwe Schlomann, Douglas A. Lauffenburger 19. Synthesis and Application of Activity-Based Probes for Proteases Tim Van Kersavond, Minh T. N. Nguyen, and Steven H. L. Verhelst
Автор: Hicham Zegzouti; Said A. Goueli Название: Kinase Screening and Profiling ISBN: 1493949233 ISBN-13(EAN): 9781493949236 Издательство: Springer Рейтинг: Цена: 13275.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
1. HTRF Kinase Assay Development and Methods in Inhibitor Characterization Yong Jia, Mari Manuia, and Jose Juarez
2. Application of Eukaryotic Elongation Factor-2 Kinase (eEF-2K) for Cancer Therapy: Expression, Purification, and High-Throughput Inhibitor Screening Clint D. J. Tavares, Ashwini K. Devkota, Kevin N. Dalby, and Eun Jeong Cho 3. Recombinant Kinase Production and Fragment Screening by NMR Spectroscopy Byeonggu Han and Hee-Chul Ahn 4. Bioluminescence Methods for Assaying Kinases in Quantitative High-Throughput Screening (qHTS) Format Applied to Yes1 Tyrosine Kinase, Glucokinase and PI5P4Kα Lipid Kinase Mindy I. Davis, Douglas S. Auld, and James Inglese 5. Using Bioluminescent Kinase Profiling Strips to Identify Kinase Inhibitor Selectivity and Promiscuity Hicham Zegzouti, Jacquelyn Hennek, and Said A. Goueli 6. Measuring Activity of Phosphoinositide Lipid Kinases Using a Bioluminescent ADP-Detecting Assay Andrew W. Tai and Jolanta Vidugiriene
7. A High-Throughput Radiometric Kinase Assay Krisna C. Duong-Ly and Jeffrey R. Peterson 8. A High Content Assay to Screen for Modulators of EGFR Function Christophe Antczak and Hakim Djaballah 9. Monitoring Protein Kinase Expression and Phosphorylation in Cell Lysates with Antibody Microarrays Hong Zhang, Xiaoqing Shi, and Steven Pelech
10. From Enzyme to Whole Blood: Sequential Screening Procedure for Identification and Evaluation of p38 MAPK Inhibitors Silke M. Bauer, Jakub M. Kubiak, Ulrich Rothbauer, and Stefan Laufer
11. Genetically Encoded Fluorescent Indicators to Visualize Protein Phosphorylation in Living Cells Moritoshi Sato and Yoshio Umezawa
12. Characterization of an Engineered Src Kinase to Study Src Signaling and Biology Leanna R. Gentry, Andrei V. Karginov, Klaus M. Hahn, and Channing J. Der
13. Screening One-Bead-One-Compound Peptide Libraries for Optimal Kinase Substrates Thi B. Trinh and Dehua Pei
14. Determination of the Substrate Specificity of Protein Kinases with Peptide Micro- and Macroarrays Shenshen Lai, Dirk F.H. Winkler, Hong Zhang, and Steven Pelech 15. Rapid Identification of Protein Kinase Phosphorylation Site Motifs Using Combinatorial Peptide Libraries Chad J. Miller and Benjamin E. Turk
Автор: Anton Posch Название: Proteomic Profiling ISBN: 1493925490 ISBN-13(EAN): 9781493925490 Издательство: Springer Рейтинг: Цена: 30745.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume presents the latest developments of the main pillars of protein analysis, such as sample preparation, separation and characterization. The book begins by describing basic but important sample preparation protocols. It then goes on to describe more sophisticated procedures on enriching specific protein classes and concludes with detailed descriptions of integrated work-flows for comprehensive protein analysis and characterization. The authors of the individual chapters are renowned protein biochemists who have all set value to provide a detailed representation of their lab work. Throughout the chapters, these authors share important tips and tricks for a successful and reproducible employment of their protocols in other laboratories. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Proteomic Profiling: Methods and Protocols is the perfect guide for students of Biochemistry, Biomedicine, Biology, and Genomics and will be an invaluable source for the experienced, practicing scientists.
Автор: Stephan A. Sieber Название: Activity-Based Protein Profiling ISBN: 3642448313 ISBN-13(EAN): 9783642448317 Издательство: Springer Рейтинг: Цена: 36570.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: SieberActivity-Based Protein Profiling for Natural Product Target Discovery, by Joanna Krysiak und Rolf BreinbauerPhotoaffinity Labeling in Activity-Based Protein Profiling, by Paul P. TateFunctional Analysis of Protein Targets by Metabolomic Approaches, by Yun-Gon Kim und Alan Saghatelian
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