The ABCs of Gene Cloning, Wong

Автор: P.H. Hofschneider; W. Goebel
Название: Gene Cloning in Organisms Other Than E. coli
ISBN: 3642683177 ISBN-13(EAN): 9783642683176
Издательство: Springer
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Описание: Gene cloning for the production of recombinant DNA is usually performed with E. coli. There is, however, no doubt that gene cloning in organisms other than E. coli will assume a much more important role in the future: efficient cloning systems are needed for the analysis of gene expression and its regulation in eukaryotic cells, for the elucidation ofthe genetic mechanisms of transform a- tion, and for the study of the genetic rearrangement during dif- ferentiation and embryogenesis. Furthermore, optimal cloning systems will be required for practical applications in the near future: for the optimal production of antibiotics, amino acids, vitamins, enzymes etc., for the expression of gene products under favorable energetic conditions for mass production, for the ex- pression of glycolysated animal proteins, for the genetic manipu- 'lation of plants -and in the more distant future, for gene therapy inman. The editors have therefore invited leading scientists to sum- marize the present status and the future applicability of gene cloning systems in their fields of research. To ensure a complete coverage of a genetic system, the editors have generally asked two authors to cover one system. This volume is a frrst and unique account of our knowledge of cloning systems employing organisms other than E. coli such as B. subtilis, pseudomonas, neurospora, saccharomyces and streptomyces, as well as of vectors and selection procedures for animal and plant systems including the liposome technique. October 1981 P. R.

Автор: David M. Glover
Название: Gene Cloning
ISBN: 0412266008 ISBN-13(EAN): 9780412266003
Издательство: Springer
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Описание: This book was originallyconceived in the form ofa second edition ofa volume published in 1980 in Chapman and Hall's 'OutllneStudies in Biology' series and entitled Genetic Engineering - Cloning DNA.It very rapidly became apparent that with the impact ofrecombinant DNA techniques being feIt in so many areas ofblology, it was going to be difficultifnotimpossible to keepthe bookwithin the space confines of these little monographs. The stays were therefore loosened and the book expanded comfortably to its present size.I hope that this extra space has allowed me to clarify sections ofthe text that were 'heavy going' in the earlierversion.Theextraspace has certainlyallowed me to cover topics that were not mentioned at all in the earlier book. These are primarily to be found in Chapters 7 and 8, which cover the rapid advances that have been recently made in the use ofplantand animal cells as hosts for recombinant DNAmolecules. The develop- ment ofother vectors has certainly not stood still over the past four years.This has necessitated a thorough revision ofChapters 3 and 4, which deal with bacteriophage and bacterial plasmid vectors. Numerous techniques for in vitromutagenesis have now been tried and tested allowing me to givecomprehensive coverage ofthisarea in Chapter 2 along with the biochemical techniques used to construct recombinant DNA molecules. Readers with some background knowledge of the approaches to gene cloning will be able to go straight toapart ofthe book in whichthey are specificallyinterested.

Автор: Sunil Chandran; Kevin W. George
Название: DNA Cloning and Assembly
ISBN: 1071609076 ISBN-13(EAN): 9781071609071
Издательство: Springer
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Описание: This volume provides a comprehensive collection of DNA assembly protocols that will prove useful for any researcher interested in molecular cloning, synthetic biology, or DNA manipulation.

Название: Dna cloning and assembly methods
ISBN: 1627037632 ISBN-13(EAN): 9781627037631
Издательство: Springer
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Описание: In DNA Cloning and Assembly Methods, expert researchers in the field detail many of the methods which are now commonly used for DNA cloning and make cloning procedures faster, more reliable and also suitable for high-throughput handling.

Автор: Seok-Yong Choi; Hyunju Ro; Hankuil Yi
Название: DNA Cloning: A Hands-on Approach
ISBN: 9402416609 ISBN-13(EAN): 9789402416602
Издательство: Springer
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Описание: This book offers step-by-step instruction on DNA cloning, defined as moving genes around plasmids, mutating genes, or mining new genes. The aim is to provide those new to the field with reliable and up-to-date practical guidance while at the same time conveying the scope for creativity. After a brief synopsis of the history of cloning, the fundamentals and prerequisites are explained, covering, for example, software, vectors commonly used in the lab, appropriate choice of restriction endonucleases, the preparation of agarose gels, competent cells, and LB agar plates, and procedures to be followed upon receipt of new plasmids. The remainder of the book is devoted to the clear description of methods and individual steps in cloning. Guidance is provided on the cut and paste method, DNA sequencing, direct sequencing, primer design, PCR-based gene insertion and deletion, epitope tag insertion, the use of RACE technology, BAC recombineering, and much, much more. Sources of error and a variety of techniques that make life considerably easier when cloning are also examined in detail.

Автор: Robert W. Sussman; C. Robert Cloninger
Название: Origins of Altruism and Cooperation
ISBN: 1461429862 ISBN-13(EAN): 9781461429869
Издательство: Springer
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Описание: This book examines the evolution and nature of cooperation and altruism in social-living animals, focusing especially on non-human primates and humans. It is derived from a conference held at Washington University, March, 2009.

Автор: Svein Valla; Rahmi Lale
Название: DNA Cloning and Assembly Methods
ISBN: 1493960571 ISBN-13(EAN): 9781493960576
Издательство: Springer
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Описание: In DNA Cloning and Assembly Methods, expert researchers in the field detail many of the methods which are now commonly used for DNA cloning and make cloning procedures faster, more reliable and also suitable for high-throughput handling.