DNA Cloning: A Hands-on Approach, Seok-Yong Choi; Hyunju Ro; Hankuil Yi

Автор: Svein Valla; Rahmi Lale
Название: DNA Cloning and Assembly Methods
ISBN: 1493960571 ISBN-13(EAN): 9781493960576
Издательство: Springer
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Описание: In DNA Cloning and Assembly Methods, expert researchers in the field detail many of the methods which are now commonly used for DNA cloning and make cloning procedures faster, more reliable and also suitable for high-throughput handling.

Название: Dna cloning and assembly methods
ISBN: 1627037632 ISBN-13(EAN): 9781627037631
Издательство: Springer
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Цена: 23757.00 р.
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Описание: In DNA Cloning and Assembly Methods, expert researchers in the field detail many of the methods which are now commonly used for DNA cloning and make cloning procedures faster, more reliable and also suitable for high-throughput handling.

Автор: Wong
Название: The ABCs of Gene Cloning
ISBN: 3319777629 ISBN-13(EAN): 9783319777627
Издательство: Springer
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Описание: Clear and concise, this easy-to-use book offers an introductory course on the language of gene cloning, covering microbial, plant, and mammalian systems. It presents the nuts and bolts of gene cloning in a well-organized and accessible manner. Part I of this book outlines the essentials of biology and genetics relevant to the concept of gene cloning. Part II describes common techniques and approaches of gene cloning, ranging from the basic mechanics of DNA manipulation, vector systems, process transformation, to gene analysis. Part III & IV present application technologies of major impact in agriculture, biomedicine, and related areas. The ABCs of Gene Cloning, Third Edition contains updates including a tutorial chapter on gene-vector construction, methodologies on exome sequencing in finding disease genes, revised topics on gene therapy and whole genome sequencing, new developments for gene targeting and genome editing, as well as the current state of next generation sequencing. With more than 140 illustrations, this new edition provides an invaluable text for students and anyone who have interest in gaining proficiency in reading and speaking the language of gene cloning.

Автор: David M. Glover
Название: Gene Cloning
ISBN: 0412266008 ISBN-13(EAN): 9780412266003
Издательство: Springer
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Описание: This book was originallyconceived in the form ofa second edition ofa volume published in 1980 in Chapman and Hall's 'OutllneStudies in Biology' series and entitled Genetic Engineering - Cloning DNA.It very rapidly became apparent that with the impact ofrecombinant DNA techniques being feIt in so many areas ofblology, it was going to be difficultifnotimpossible to keepthe bookwithin the space confines of these little monographs. The stays were therefore loosened and the book expanded comfortably to its present size.I hope that this extra space has allowed me to clarify sections ofthe text that were 'heavy going' in the earlierversion.Theextraspace has certainlyallowed me to cover topics that were not mentioned at all in the earlier book. These are primarily to be found in Chapters 7 and 8, which cover the rapid advances that have been recently made in the use ofplantand animal cells as hosts for recombinant DNAmolecules. The develop- ment ofother vectors has certainly not stood still over the past four years.This has necessitated a thorough revision ofChapters 3 and 4, which deal with bacteriophage and bacterial plasmid vectors. Numerous techniques for in vitromutagenesis have now been tried and tested allowing me to givecomprehensive coverage ofthisarea in Chapter 2 along with the biochemical techniques used to construct recombinant DNA molecules. Readers with some background knowledge of the approaches to gene cloning will be able to go straight toapart ofthe book in whichthey are specificallyinterested.

Автор: Faraday Patrick
Название: Gene Cloning
ISBN: 1682865150 ISBN-13(EAN): 9781682865156
Издательство: Неизвестно
Цена: 24299.00 р.
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Автор: Valdis Berzins
Название: Basic Cloning Procedures
ISBN: 364248977X ISBN-13(EAN): 9783642489778
Издательство: Springer
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Цена: 6986.00 р.
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Автор: Joel F. Habener
Название: Molecular Cloning of Hormone Genes
ISBN: 0896030911 ISBN-13(EAN): 9780896030916
Издательство: Springer
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Цена: 34799.00 р.
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Описание: In addition to provid- ing the coding sequences of recognized hormonal and regulatory peptides, gene sequencing has uncovered new bioactive peptides encoded in the precursor pro hormones that are then liberated along with the hormonal peptides during cellular cleavages of the precursors.

Автор: Hardin
Название: Cloning Gene Expression
ISBN: 0195132947 ISBN-13(EAN): 9780195132946
Издательство: Oxford Academ
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Описание: The central purpose of this combination lecture/laboratory manual is to present detailed protocols for the multi-step process involved in isolating a gene, cloning and characterizing it, expressing the encoded protein, purifying the protein, and characterizing rudimentary aspects of its basic physical properties. The manual includes 20 experiments designed to train students to prepare, manipulate, and analyzse plasmids, to produce fusion proteins in bacteria, and to purify these proteins based on unique chemical properties or substrate affinities. Electrophoresis, southern and western blotting and cominatorial techniques are empsized. Radioisotopes, fluorophores and solvent effects on protein structure are discussed. (No radioisotopes are required.) The experiments included have been selected because of their high success rate and because they emphasize a project-oriented approach. Sufficient detail and background are provided such that the intended audience includes advanced undergraduate students, beginning graduates, and practising professionals engaged in a broad range of pursuits. Each experiment is accompanied by detailed process rationale.

Автор: Robert W. Sussman; C. Robert Cloninger
Название: Origins of Altruism and Cooperation
ISBN: 1461429862 ISBN-13(EAN): 9781461429869
Издательство: Springer
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Цена: 23508.00 р.
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Описание: This book examines the evolution and nature of cooperation and altruism in social-living animals, focusing especially on non-human primates and humans. It is derived from a conference held at Washington University, March, 2009.

Автор: P.H. Hofschneider; W. Goebel
Название: Gene Cloning in Organisms Other Than E. coli
ISBN: 3642683177 ISBN-13(EAN): 9783642683176
Издательство: Springer
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Цена: 18167.00 р.
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Описание: Gene cloning for the production of recombinant DNA is usually performed with E. coli. There is, however, no doubt that gene cloning in organisms other than E. coli will assume a much more important role in the future: efficient cloning systems are needed for the analysis of gene expression and its regulation in eukaryotic cells, for the elucidation ofthe genetic mechanisms of transform a- tion, and for the study of the genetic rearrangement during dif- ferentiation and embryogenesis. Furthermore, optimal cloning systems will be required for practical applications in the near future: for the optimal production of antibiotics, amino acids, vitamins, enzymes etc., for the expression of gene products under favorable energetic conditions for mass production, for the ex- pression of glycolysated animal proteins, for the genetic manipu- 'lation of plants -and in the more distant future, for gene therapy inman. The editors have therefore invited leading scientists to sum- marize the present status and the future applicability of gene cloning systems in their fields of research. To ensure a complete coverage of a genetic system, the editors have generally asked two authors to cover one system. This volume is a frrst and unique account of our knowledge of cloning systems employing organisms other than E. coli such as B. subtilis, pseudomonas, neurospora, saccharomyces and streptomyces, as well as of vectors and selection procedures for animal and plant systems including the liposome technique. October 1981 P. R.

Автор: Shiv Sanjeevi, Prerna Pandey
Название: Cloning in plants: new developments
ISBN: 1774071762 ISBN-13(EAN): 9781774071762
Издательство: Mare Nostrum (Eurospan)
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Цена: 24671.00 р.
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Описание: Examines the process of cloning and plant transformation. The book covers the tools required for cloning. The basic requirements of cloning, and cloning and transformation of medicinal plants is also described.