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Enzyme-Mediated Ligation Methods, Timo Nuijens; Marcel Schmidt


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Автор: Timo Nuijens; Marcel Schmidt
Название:  Enzyme-Mediated Ligation Methods
ISBN: 9781493995455
Издательство: Springer
Классификация:




ISBN-10: 1493995456
Обложка/Формат: Hardcover
Страницы: 371
Вес: 0.94 кг.
Дата издания: 2019
Серия: Methods in Molecular Biology
Язык: English
Издание: 1st ed. 2019
Иллюстрации: 59 illustrations, color; 35 illustrations, black and white; xi, 371 p. 94 illus., 59 illus. in color.
Размер: 254 x 178
Читательская аудитория: Professional & vocational
Основная тема: Chemistry
Ссылка на Издательство: Link
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Поставляется из: Германии
Описание: This volume discusses different enzyme-catalyzed ligation methodologies for a variety of different chemical transformations. This book wants readers to view enzymes as a powerful tool in both academic and industrial research. Chapters in this book cover topics such as sortase A-mediated generation of site-specifically conjugated antibody-drug conjugates; omniligase-catalyzed inter- and intramolecular ligation; ligation catalyzed by microbial transglutaminase; peptide cyclization mediated by cyanobactin macrocyclases, butelase 1 and sortase A; using BioID as a tool for protein proximity labeling in living cells; and inducible, selective labeling of proteins via enzymatic oxidation of tyrosine. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and thorough, Enzyme-Mediated Ligation Methods is a valuable resource for students and scientists from different disciplines who are interested in using enzymatic strategies to answer their research questions.

Дополнительное описание: Sortase A Enzyme-Mediated Generation of Site-Specifically Conjugated Antibody-Drug Conjugates.- Site-Specific C-Terminal Labeling of Recombinant Proteins with Proximity-Based Sortase-Mediated Ligation (PBSL).- Cyclizing Disulfide-Rich Peptides using Sorta



Phosphoinositides in Subcellular Targeting and Enzyme Activation

Автор: Stenmark Harald
Название: Phosphoinositides in Subcellular Targeting and Enzyme Activation
ISBN: 3540009507 ISBN-13(EAN): 9783540009504
Издательство: Springer
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Цена: 1092.00 р.
Наличие на складе: Есть (3 шт.)
Описание: Cells of the immune system are activated by a variety of stimuli that are derived from other cells, ingested material or from invading microorganisms. This issue of CTMI focuses on the mechanisms of phosphoinositide-mediated protein recruitment to intracellular membranes.

Protein Ligation and Total Synthesis I

Автор: Lei Liu
Название: Protein Ligation and Total Synthesis I
ISBN: 3319359746 ISBN-13(EAN): 9783319359748
Издательство: Springer
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Цена: 28732.00 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание: Each review within the volume critically surveys one aspect of that topic and places it within the context of the volume as a whole.

Protein Ligation and Total Synthesis II

Автор: Lei Liu
Название: Protein Ligation and Total Synthesis II
ISBN: 3319191888 ISBN-13(EAN): 9783319191881
Издательство: Springer
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Цена: 34937.00 р.
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Описание: Each review within the volume critically surveys one aspect of that topic and places it within the context of the volume as a whole. The most significant developments of the last 5 to 10 years are presented using selected examples to illustrate the principles discussed. The coverage is not intended to be an exhaustive summary of the field or include large quantities of data, but should rather be conceptual, concentrating on the methodological thinking that will allow the non-specialist reader to understand the information presented. Contributions also offer an outlook on potential future developments in the field.

Protein Ligation and Total Synthesis I

Автор: Lei Liu
Название: Protein Ligation and Total Synthesis I
ISBN: 3319191853 ISBN-13(EAN): 9783319191850
Издательство: Springer
Рейтинг:
Цена: 34937.00 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание: Each review within the volume critically surveys one aspect of that topic and places it within the context of the volume as a whole. The most significant developments of the last 5 to 10 years are presented using selected examples to illustrate the principles discussed. The coverage is not intended to be an exhaustive summary of the field or include large quantities of data, but should rather be conceptual, concentrating on the methodological thinking that will allow the non-specialist reader to understand the information presented. Contributions also offer an outlook on potential future developments in the field.

Protein Ligation and Total Synthesis II

Автор: Lei Liu
Название: Protein Ligation and Total Synthesis II
ISBN: 3319369377 ISBN-13(EAN): 9783319369372
Издательство: Springer
Рейтинг:
Цена: 28732.00 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание: Each review within the volume critically surveys one aspect of that topic and places it within the context of the volume as a whole.

Soil Enzymes. Influence of Sugar Industry Effluents on Soil Enzyme Activities

Автор: Maddela
Название: Soil Enzymes. Influence of Sugar Industry Effluents on Soil Enzyme Activities
ISBN: 3319426540 ISBN-13(EAN): 9783319426549
Издательство: Springer
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Цена: 7685.00 р.
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Описание: This book addresses issues arising from discharge of effluents from sugar industry on to surrounding land or into a water body such as physicochemical properties of soil, changes in the micro flora, quantification of soil enzyme activities as influenced by effluents. Disposal of effluents without neutralization has become general practice. These effluents are chemically heterogeneous, contain organic and inorganic pollutants including, sugar baggage, molasses, carbonates, bicarbonates. The impact of sugar industry effluents on microbial activities in terrestrial ecosystem is scanty. There is also significant interest in the study of soil enzymes because such effect reflects the potential capacity of a soil to perform certain biological transformation of soil fertility.

Enzyme-Mediated Immunoassay

Автор: H.M. Lenhoff; That T. Ngo
Название: Enzyme-Mediated Immunoassay
ISBN: 146845014X ISBN-13(EAN): 9781468450149
Издательство: Springer
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Цена: 6986.00 р.
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Описание: Their method depends upon ~ competition between insulin labeled with radioactive iodine (II 1) and unlabeled insulin from plasma for a fixed and limited number of specific binding sites on the antibody to insulin.

Enzyme Stabilization and Immobilization

Автор: Shelley D. Minteer
Название: Enzyme Stabilization and Immobilization
ISBN: 1493964976 ISBN-13(EAN): 9781493964970
Издательство: Springer
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Цена: 19564.00 р.
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Описание: This volume introduces the reader to the field of enzyme stabilization and the different theories of enzyme stabilization, including the use of immobilization as a stabilization technique. The first part of the book focuses on protocols for enzyme stabilization in solutions including liposome formation, micelle introduction, crosslinking, and additives. The second part of the book discusses protocols for enzyme stabilization during enzyme immobilization, including common techniques like sol-gel encapsulation, polymer encapsulation, and single enzyme nanoparticle formation. Protocols for a variety of enzymes are shown, but the enzymes are chosen as examples to show that these protocols can be used for both enzymes of biological importance, as well as enzymes of industrial importance. The final part details spectroscopic protocols, methods, and assays for studying the effectiveness of the enzyme stabilization and immobilization strategies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Enzyme Stabilization and Immobilization: Methods and Protocols, Second Edition provides molecular biologists, biochemists, and biomedical and biochemical engineers with the state-of-the-art technical information required to effectively stabilize their enzyme of interest in a variety of environments (i.e., harsh temperature, pH, or solvent conditions).

Enzyme- and Transporter-Based Drug-Drug Interactions

Автор: K. Sandy Pang; A. David Rodrigues; Raimund M. Pete
Название: Enzyme- and Transporter-Based Drug-Drug Interactions
ISBN: 1489994890 ISBN-13(EAN): 9781489994899
Издательство: Springer
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Цена: 28732.00 р.
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Описание: This proceedings volume from the annual meeting of The American Association of Pharmaceutical Scientists, held November 2007, examines enzyme- and transporter-based DDIs that impact the absorption and disposition of drugs and impact on adverse outcomes.


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