The Plant Cell Wall: Methods and Protocols, Popper Zoл a.
Автор: Bayer Martin Название: Plant Embryogenesis: Methods and Protocols ISBN: 1071603418 ISBN-13(EAN): 9781071603413 Издательство: Springer Рейтинг: Цена: 16769.00 р. Наличие на складе: Поставка под заказ.
Описание: This volume details state-of-the-art methods for the study of plant embryogenesis in the model organism Arabidopsis thaliana, other models, and non-model species.
Автор: Kleine-Vehn Jьrgen, Sauer Michael Название: Plant Hormones: Methods and Protocols ISBN: 1493982109 ISBN-13(EAN): 9781493982103 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
Real Time Analysis of the Apical Hook Development.- Grafting with Arabidopsis thaliana.- Tips and Tricks for Exogenous Application of Synthetic Post-Translationally Modified Peptides to Plants.- Assaying Germination and Seedling Responses of Arabidopsis to Karrikins.- Low-cost Microprocessor-Controlled Rotating Stage for Medium-Throughput Time-Lapse Plant Phenotyping.- Genome-Wide Association Mapping of Root Traits in the Context of Plant Hormone Research.- High throughput scoring of Seed Germination.- Histochemical Staining of β-Glucuronidase and its Spatial Quantification.- Imaging TCSn:: GFP, A Synthetic Cytokinin Reporter, in Arabidopsis thaliana.- Highlighting Gibberellins Accumulation Sites in Arabidopsis Thaliana Root Using Fluorescently Labeled Gibberellins.- In-silico Methods for Cell Annotation, Quantification of Gene Expression and Cell Geometry at Single Cell Resolution using 3DCellAtlas.- Analysing Cell Wall Elasticity after Hormone Treatment: An example using Tobacco BY-2 Cells and Auzin.- FRET-FLIM for Visualizing and Quantifying Protein Interactions in Live Plant Cells.- In vivo Identification of Plant Protein Complexes using IP-MS/MS.- Assaying auxin Receptor Activity using SPR assays with F-Box Proteins and Aux-IAA degrons.- Studying Transcription Factor Binding To Specific Genomic Loci by Chromatin Immunoprecipitation (ChIP).- Hormone Receptor Glycosylation.- Highly Sensitive Salicylic Acid Quantification in Milligram Amounts of Plant Tissue.- High-Resolution Cell-Type Specific Analysis of Cytokinins in Sorted Root Cell Populations of Arabidopsis thaliana.- Hormone Profiling in Plant Tissues.- Use of Xenopus laevis Oocytes to Study Auxin Transport.- Characterizing Auxin Response Circuits in Saccharomyces cerevisiae by Flow Cytometry.
Автор: Dandekar Thomas, Naseem Muhammad Название: Auxins and Cytokinins in Plant Biology: Methods and Protocols ISBN: 1493983113 ISBN-13(EAN): 9781493983117 Издательство: Springer Рейтинг: Цена: 13275.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
Preface... Table of Contents... Contributing Authors...
Methodological Advances in Auxin and Cytokinin Biology Andrej Hurnэ and Eva Benkovб
Analytical Determination of Auxins and Cytokinins Petre I. Dobrev, Klбra Hoyerovб, and Jan Petrбsek
Manipulation of Auxin and Cytokinin Balance During the Plasmodiophora brassicae: Arabidopsis thaliana Interaction Jutta Ludwig-Mьller, Susann Auer, Sabine Jьlke, and Sabine Marschollek
Microbial Manipulation of Auxin and Cytokinins in Plants Anwar Hussain, Uhsan Ullah, and Shahida Hasnain
A Standardized Method to Assess Infection Rates of Root-Knot and Cyst Nematodes in Arabidopsis thaliana Mutants with Alterations in Root Development Related to Auxin and Cytokinin Signaling Rocнo Olmo, Ana Clбudia Silva, Fernando E. Dнaz-Manzano, Javier Cabrera, Carmen Fenoll, and Carolina Escobar
Reconstruction of an Immune Dynamic Model to Simulate the Contrasting Role of Auxin and Cytokinin in Plant Immunity Martin Kaltdorf, Dandekar Thomas, and Muhammad Naseem
Interplay between Auxin and Cytokinin and its Impact on Mitogen Activated Protein Kinase (MAPK) Pallavi Singh and Alok Krishna Sinha
Quantification of Cytokinin Levels and Responses in Abiotic Stresses Alfonso Albacete
Assessment of Cytokinin-Induced Immunity through Quantification of Hyaloperonospora arabidopsidis Infection in Arabidopsis thaliana Ruth A. Watson and Cris T. Argueso
Real-Time Genetic Manipulations of the Cytokinin Pathway: A Tool for Laboratory and Field Studies Martin Schдfer and Stefan Meldau
Modulating the Levels of Plant Hormone Cytokinins at the Host-Pathogen Interface Muhammad Naseem, Shabana Shams, and Thomas Roitsch
Analyzing Cytokinin Responses during Plant-Nematode Interactions Florian M.W. Grundler and Shahid Siddique
Examining H2O2 Production in Arabidopsis Leaves Upon Challenge by Cytokinin Shahjahan Shabbir Ahmed, Ihsan Ullah, Shazia Irfan, and Nazeer Ahmed
A Systems Biology Methodology Combining Transcriptome and Interactome Datasets to Assess the Implications of Cytokinin Signaling for Plant Immune Networks Meik Kunz, Thomas Dandekar, and Muhammad Naseem
Monitoring of Crosstalk between Jasmonate and Auxin in the Framework of Plant Stress Responses of Roots Vнctor Carrasco Loba, Marta-Marina Pйrez Alonso, and Stephan Pollmann
High-Throughput Protoplast Trans-Activation (PTA) Screening to Define Transcription Factors in Auxin-Mediated Gene Regulation Nora Whner, Jцrn Herfert, Wolfgang Drцge-Laser, and Christoph Weiste
Автор: Spillane Charles, McKeown Peter Название: Plant Epigenetics and Epigenomics: Methods and Protocols ISBN: 1071601784 ISBN-13(EAN): 9781071601785 Издательство: Springer Рейтинг: Цена: 16769.00 р. Наличие на складе: Поставка под заказ.
Описание: This second edition volume expands on the previous edition with a look at the latest techniques in plant epigenetics and epigenomic research. Chapters in this book cover topics such as whole genome methylome analysis; analysis of DNA methylation; chromatin analysis of metabolic gene clusters in plants; plant epigenetic stress memory induced by drought; small non-coding RNA analysis in seeds of forest tree species; and a discussion on epigenetics inheritance in plants. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Plant Epigenetics and Epigenomics: Methods and Protocols, Second Edition is a valuable resource for both novice and expert researchers, and aims to advance this field of study by paving the way for integrating epigenetic mechanisms into models of plant function during development and evolution.
Описание: This volume provides readers with wide-ranging coverage of CRISPR systems and their applications in various plant species. The chapters in this book discuss topics such as plant DNA repair and genome editing; analysis of CRISPR-induced mutations; multiplexed CRISPR/Cas9 systems; CRISPR-Cas12a (Cpf1) editing systems; and non-agrobacterium based CRISPR delivery systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Comprehensive and thorough, Plant Genome Editing with CRISPR Systems: Methods and Protocols is a valuable resource for any researcher interested in learning about and using CRISPR systems in plants.
Автор: Van Dijk Aalt D. J. Название: Plant Genomics Databases: Methods and Protocols ISBN: 149398263X ISBN-13(EAN): 9781493982639 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
Preface... Table of Contents... Contributing Authors...
1. Ensembl Plants: Integrating Tools for Visualizing, Mining and Analyzing Plant Genomics Data Dan M. Bolser, Daniel M. Staines, Emily Perry, and Paul J. Kersey
2. PGSB/MIPS PlantsDB Database Framework for the Integration and Analysis of Plant Genome Data Manuel Spannagl, Thomas Nussbaumer, Kai Bader, Heidrun Gundlach, and Klaus FX Mayer 3. Plant Genome DataBase Japan (PGDBj) Akihiro Nakaya, Hisako Ichihara, Erika Asamizu, Sachiko Shirasawa, Yasukazu Makamura, Satoshi Tabata, and Hideki Hirakawa 4. FLAGdb++: A Bioinformatic Environment to Study and Compare Plant Genomes Jean Philippe Tamby and Vйronique Brunaud 5. Mining Plant Genomic and Genetic Data Using the GnpIS Information System A-F Adam-Blondon, M. Alaux, S. Durand, T. Letellier, G. Merceron, N. Mohellibi, C. Pommier, D. Steinbach, F. Alfama, J. Amselem, D. Charruaud, N. Choisne, R. Flores, C. Guerche, V. Jamilloux, E. Kimmel, N. Lapalu, M. Loaec, C. Michotey, H. Quesneville 6. The Bio-Analytic Resource for Plant Biology Jamie Waese and Nicholas J. Provart 7. The Evolution of Soybean Knowledge Base (SoyKB) Trupti Joshi, Jiaojiao Wang, Hongxin Zhang, Shiyuan Chen, Shuai Zeng, Bowei Xu, and Dong Xu 8. Using TropGeneDB: a Database Containing Data on Molecular Markers, QTLs, Maps, Genotypes, and Phenotypes for Tropical Crops Manuel Ruiz, Guilhem Sempere, and Chantal Hamelin 9. Species-Specific Genome Sequence Databases: A Practical Review A.D.J. van Dijk 10. A Guide to the PLAZA 3.0 Plant Comparative Genomics Database Klaas Vandepoele 11. Exploring Plant Co-Expression and Gene-Gene Interactions with CORNET 3.0 Michiel Van Bel and Frederik Coppens 12. PlaNet: Comparative Co-Expression Network Analyses for Plants Sebastian Proost and Marek Mutwil 13. Practical Utilization of OryzaExpress and Plant Omics Data Center Databases to Explore Gene Expression Networks in Oryza sativa and Other Plant Species Toru Kudo, Shin Terashima, Yuno Takaki, Yukino Nakamura, Masaaki Kobayashi, and Kentaro Yano 14. Pathway Analysis and Omics Data Visualization using Pathway Genome Databases: FragariaCyc, A Case Study Sushma Naithani and Pankaj Jaiswal 15. CSGRqtl: A Comparative Quantitative Trait Locus Database for Saccharinae Grasses Dong Zhang and Andrew H. Paterson 16. Plant Genome Duplication Database Tae-Ho Lee, Junah Kim, Jon S. Robertson, and Andrew H. Paterson 17. Variant Effect Prediction Analysis using Resources Available at Gramene Database Sushma Naithani, Matt Geniza, and Pankaj Jaiswal 18. Plant Promoter Database Kazutaka Kusunoki and Yoshiharu Y. Yamamoto 19. Construction of the Leaf Senescence Database and Functional Assessment of Senescence-Associated Genes Zhonghai Li, Yi Zhao, Xiaochuan Liu, Zhiqiang Jiang, Jinying Peng, Jinpu Jin, Hongwei Guo, and Jingchu Luo
Автор: Dissmeyer Nico, Schnittger Arp Название: Plant Kinases: Methods and Protocols ISBN: 149395721X ISBN-13(EAN): 9781493957217 Издательство: Springer Рейтинг: Цена: 16769.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Modification of target protein properties by reversible phosphorylation events has been found to be one of the most prominent cellular control processes in all organisms. Recent advances in the areas of molecular biology and biochemistry are presenting new possibilities for reaching an unprecedented depth and a proteome-wide understanding of phosphorylation processes in plants as well as in other species. The major goal of Plant Kinases: Methods and Protocols is to provide the experimentalist with a detailed account of the practical steps necessary for successfully carrying out each protocol in his or her own laboratory. Plant protein kinases specifically addressed in this volume are members of the plant MAP kinase cascade, cyclin- and Calcium-dependent protein kinases, and plant sensor and receptor kinases. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Plant Kinases: Methods and Protocols will prove a useful laboratory companion to both novice and seasoned researchers by facilitating the practical work that will lead them to new and exciting insights in this dynamic field.
Автор: Taylor Nicolas L., Millar A. Harvey Название: Isolation of Plant Organelles and Structures: Methods and Protocols ISBN: 1493982281 ISBN-13(EAN): 9781493982288 Издательство: Springer Рейтинг: Цена: 16070.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This book brings together the major techniques used in the isolation or enrichment of individual populations of organelles and other subcellular structures from plants with the goal that, by being able to isolate subcellular structures, the research and understanding of various facets of compartmentalized function in plant cells can be advanced.
Автор: Yuan Ling, Perry Sharyn E. Название: Plant Transcription Factors: Methods and Protocols ISBN: 1493958445 ISBN-13(EAN): 9781493958443 Издательство: Springer Рейтинг: Цена: 20263.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Recent years have seen significant advancements in the development of enabling technologies that facilitate the study of Transcription Factors (TFs). TFs are pivotal in the regulation of plant development, reproduction, intercellular signaling, response to environment, cell cycle, and metabolism. Plant Transcription Factors: Methods and Protocols offers a comprehensive approach by covering the basic concepts as well as the detailed protocols of a series of commonly used tools for investigating plant TFs. From discussing select TF families in plants to presenting approaches for identifying them, methods are covered to verify the function, to identify protein interactions in which TFs are involved, and how the interactions are mediated. Increasing examples of TFs that function non-cell-autonomously are being discovered and methods to assess intercellular trafficking are also addressed. A section is devoted to examining interaction with DNA, and the volume concludes with a discussion of directed evolution to generate transcription factors that can more efficiently control desired processes. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Plant Transcription Factors: Methods and Protocols serves as an ideal guide to seasoned plant molecular biologists as well as scientists new to the field of TFs and provides many necessary methods to all scientists who are interested in exploring the functions of transcription factors.
Автор: Gupta Kapuganti Jagadis Название: Plant Nitric Oxide: Methods and Protocols ISBN: 1493980904 ISBN-13(EAN): 9781493980901 Издательство: Springer Рейтинг: Цена: 6986.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: With numerous methods available for the quantification ofnitric oxide (NO), this detailed book explores their advantages anddisadvantages in order to provide a foundation for further research in plantnitric oxide.
Автор: Carbonell Alberto Название: Plant Argonaute Proteins: Methods and Protocols ISBN: 1493984047 ISBN-13(EAN): 9781493984046 Издательство: Springer Рейтинг: Цена: 16070.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
1. Plant ARGONAUTEs: Features, Functions and Unknowns
Alberto Carbonell
2. Analysis of the Uridylation of Both ARGONAUTE-Bound MiRNAs and 5' Cleavage Products of their Target RNAs in Plants
Guodong Ren, Xiaoyan Wang, and Bin Yu
3. In Vitro Formation of Plant RNA-Induced Silencing Complexes Using an Extract of Evacuolated Tobacco Protoplasts
Taichiro Iki, Masayuki Ishikawa, and Manabu Yoshikawa
4. In vitro Analysis of ARGONAUTE-Mediated Target Cleavage and Translational Repression in Plants
Yukihide Tomari and Hiro-oki Iwakawa
5. Establishment of an In Vivo ARGONAUTE Reporter System in Plants
Kбroly Fбtyol and Jуzsef Burgyбn
6. Immunoprecipitation and High-throughput Sequencing of ARGONAUTE-Bound RNAs from Plants
Alberto Carbonell
7. Identification of ARGONAUTE/Small RNA Cleavage Sites by Degradome Sequencing
Ivett Baksa and Gyцrgy Szittya
8. Dissecting the Sub-Nuclear Localization Patterns of ARGONAUTE Proteins and Other Components of the RNA-Directed DNA Methylation Pathway in Plants
Cheng-Guo Duan and Jian-Kang Zhu
9. Detection of ARGONAUTE 1 Association with Polysomes in Arabidopsis thaliana
Cйcile Lecampion, Elodie Lanet, and Christophe Robaglia
10. Functional Analysis of Arabidopsis ARGONAUTEs in Meiosis and DNA Repair
Marina Martinez-Garciaand Mуnica Pradillo
11. Isolation and Characterization of ARGONAUTE Mutants in Chlamydomonas
Tomohito Yamasaki
12. ARGONAUTE genes in Salvia miltiorrhiza: Identification, Characterization and Genetic Transformation
Meizhen Wang, Yuxing Deng, Fenjuan Shao, Miaomiao Liu, Yongqi Pang, Caili Li, and Shanfa Lu
13. Cloning and Characterization of ARGONAUTE Genes in Tomato
Zhiqiang Xian, Fang Yan, and Zhengguo Li
14. Non-Radioactive Detection of Small RNAs Using Digoxygenin-Labeled Probes
Ariel H. Tomassi, Delfina Gagliardi, Damian A. Cambiagno, and Pablo A. Manavella
15. Non-Radioactive Plant Small RNA Detection Using Biotin-Labeled Probes
Jun Hu and Yingguo Zhu
16. Computational Analysis of Genome-Wide ARGONAUTE-Dependent DNA Methylation in Plants
Kai Tang, Cheng-Guo Duan, Huiming Zhang and Jian-Kang Zhu
17. Structural and Functional Characterization of Plant ARGONAUTE MID Domains
Filipp Frank and Bhushan Nagar
18. Identification and Analysis of WG/GW ARGONAUTE-Binding Domains
Andrzej Zielezinski and Wojciech M. Karlowski
19. Identification and Evolutionary Characterization of ARGONAUTE-Binding Platforms
Joshua T. Trujillo and Rebecca A. Mosher
20. Phylogenetic and Evolutionary Analysis of Plant ARGONAUTES
Описание: 1. Elicitation Approaches for Withanolide Production in Hairy Root Culture of Withania somnifera (L.) Dunal Ganeshan Sivanandhan, Natesan Selvaraj, Andy Ganapathi, and Markandan Manickavasagan 2. Stimulant Paste Preparation and Bark Streak Tapping Technique for Pine Oleoresin Extraction Thanise Nogueira, Fьller, Jъlio Cйsar de Lima, Fernanco de Costa, Kelly C.S. Rodrigues-Corrкa, and Arthur G. Fett-Neto 3. A Modified Protocol for High Quality RNA Extraction from Oleoresin-Producing Adult Pines Jъlio Cйsar de Lima, Thanise Nogueira Fьller, Fernanda de Costa, Kelly C.S. Rodrigues-Corrкa, and Arthur G. Fett-Neto 4. Collection of Apoplastic Fluids from Arabidopsis thaliana Leaves Svend Roesen Madsen, Hussam Hassan Nour-Eldin, and Barbara Ann Halkier 5. From Plant Extract to a cDNA-Encoding a Glucosyltransferase Candidate: Proteomics and Transcriptomics as Tools to Help Elucidate Saponin Biosynthesis in Centella asiatica Fernando de Costa, Carla J.S. Barber, Darwin W. Reed, and Patrick S. Covello 6. Production of Recombinant Caffeine Synthase from Guarana (Paullinia cupana var. sorbillis) in Escherichia coli Flбvia Camila Schimpl, Eduardo Kiyota, and Paulo Mazzafera 7. Heterologous Expression and Characterization of Mimosinase from Leucaena leucocephala Vishal Singh Negi and Dulal Borthakur 8. Production of Aromatic Plant Terpenoids in Recombinant Baker's Yeast Anita Emmerstorfer and Harald Pichler 9. Purification of a Recombinant Polyhistidine-Tagged Glycosyltransferase Using Immobilized Metal-Affinity Chromatography (IMAC) Fernanda de Costa, Carla J.S. Barber, Pareshkumar T. Pujara, Darwin W. Reed, and Patrick S. Covello 10. A Western Blot Protocol for Detection of Proteins Heterologously Expressed in Xenopus laevis Oocytes Morten Egevang Jrgensen, Hussam Hassan Nour-Eldin, and Barbara Ann Halkier 11. Laser Capture Microdissection: Avoiding Bias in Analysis by Selecting Just What Matters Mбrcia R. de Almeida and Martina V. Strцmvik
12. Analytical and Fluorimetric Methods for the Characterization of the Transmembrane Transport of Specialized Metabolites in Plants Inкs Carqueijeiro, Viviana Martins, Henrique Noronha, Hernвni Gerуs, and Mariana Sottomayor 13. Protoplast Transformation as a Plant-Transferable Transient Expression System Patrнcia Duarte, Diana Ribeiro, Inкs Carqueijeiro, Sara Bettencourt, and Mariana Sottomayor
14. Design, Construction, and Validation of Artificial MicroRNA Vectors Using Agrobacterium-Mediated Transient Expression System Basdeo Bhagwat, Ming Chi, Dianwei Han, Haifeng Tang, Guiliang Tang, and Yu Xiang
15. Knockdown of Polyphenol Oxidase Gene Expression in Potato (Solanum tuberosum L.) with Artificial MicroRNAs Ming Chi, Basdeo Bhagwat, Guiliang Tang, and Yu Xiang
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