Hidden Markov Models: Methods and Protocols, Westhead David R., Vijayabaskar M. S.
Автор: David R. Westhead; M. S. Vijayabaskar Название: Hidden Markov Models ISBN: 1493967517 ISBN-13(EAN): 9781493967513 Издательство: Springer Рейтинг: Цена: 19564.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume aims to provide a new perspective on the broader usage of Hidden Markov Models (HMMs) in biology.
Описание: This Methods in Molecular Biology book details approaches for the detection of DNA and RNA including recovery of trace amounts of DNA for amplification and analysis, new qPCR chemistries, step-by-step, reproducible protocols, materials lists, tips and more.
Описание: This volume covers various aspects of virological research, including molecular interactions and regulatory mechanisms on the protein as well as the RNA level. It features step-by-step, readily reproducible protocols and key implementation advice.
Автор: Kelly M. Fulton (Editor), Susan M. Twine (Editor) Название: Immunoproteomics: Methods and Protocols (Methods in Molecular Biology) ISBN: 1627035885 ISBN-13(EAN): 9781627035880 Издательство: Springer Рейтинг: Цена: 23757.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Providing diverse, up-to-date approaches and techniques for studying the immunoproteome, this Methods in Molecular Biology book offers materials lists, step-by-step reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Автор: Marzia Martina; Stefano Taverna Название: Patch-Clamp Methods and Protocols ISBN: 1493910957 ISBN-13(EAN): 9781493910953 Издательство: Springer Рейтинг: Цена: 22359.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
Since its inception, patch-clamp has continued to be widely considered the gold standard method to record ion channel activity. Patch-Clamp Methods and Protocols, Second Edition, provides a comprehensive collection of new techniques for the development of automated, high-throughput screening systems for pharmacological evaluation, the use of various patch-clamp configurations together with novel molecular biological and imaging methodologies and enhanced stimulation protocols and perfusion systems. Divided into sections on pharmacology, physiology and biophysics, the chapters cover methods to generate more physiologically relevant conditions for drug application and screening technologies, recently developed applications such as optogenetic stimulation, advances in whole-cell recordings in freely-moving animals and novel technologies to create custom microelectrodes designed for reducing the access resistance and improving the rate of molecular diffusion. Patch-clamp is an indispensable technique for conducting pharmacological, physiological and biophysical research aimed at understanding crucial aspects of cellular and network function. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Patch-Cla
mp Methods and Protocols, Second Edition will provide a useful technical and methodological guide to diverse audiences of electrophysiologists, from students to experienced investigators.
Автор: Kister Alexander E. Название: Protein Supersecondary Structures: Methods and Protocols ISBN: 1493991604 ISBN-13(EAN): 9781493991600 Издательство: Springer Рейтинг: Цена: 20962.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
This second edition volume expands on the previous edition with an update on the latest developments in the field and new techniques used to study secondary and supersecondary structures (SSS) in proteins. Chapters in this book discuss topics such as sequence and structural features of different SSS elements; software used for the automatic annotation of secondary structure elements in proteins; the new developments in secondary and SSS prediction and the modern approaches for energy landscape calculation; protein misfolding and amyloid structural formation; analysis of ‘transformer proteins’; discovery of the structure of the hydrophobic nucleus; and discussions of the main principles of protein structures formation. The contributing authors of the volume are the eminent experts in the field of protein research and bioinformatics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and authoritative, Protein Supersecondary Structures: Methods and Protocols, Second Edition is a valuable resource for researchers who are interested in learning more about the relationship between amino acids sequences and protein structures, the evolution of proteins and the dynamics of protein formation.
Автор: Nicholson Sandra E., Nicola Nicos A. Название: Jak-Stat Signalling: Methods and Protocols ISBN: 1493959794 ISBN-13(EAN): 9781493959792 Издательство: Springer Рейтинг: Цена: 20263.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: The receptor-associated JAK protein kinases and their substrates, the STAT transcriptional activators, transmit signals following cytokine and growth factor binding to receptors expressed on the cell surface, to result in specific transcriptional and cellular responses. Over the last two decades, the field has progressed from identification of the individual components through to an understanding of the activation and deactivation mechanisms, and the complex structural detail of the proteins involved. We now know that these pathways are important in many biological processes, including growth and development, hematopoiesis, and the innate and adaptive immune response. JAK-STAT Signalling: Methods and Protocols provides detailed methodologies for examining many aspects of the pathway. Divided into four sections, topics include JAK and STAT specific approaches, the negative regulators of the pathway (SOCS proteins), and the production and crystallization of JAK and STAT proteins, among others. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, JAK-STAT Signalling: Methods and Protocols will be of use not only to those working in the area but also to new investigators who are led to delve into the complexities of JAK and STAT responses.
Автор: Mamitsuka Hiroshi, Delisi Charles, Kanehisa Minoru Название: Data Mining for Systems Biology: Methods and Protocols ISBN: 1493959123 ISBN-13(EAN): 9781493959129 Издательство: Springer Рейтинг: Цена: 16769.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: The post-genomic revolution is witnessing the generation of petabytes of data annually, with deep implications ranging across evolutionary theory, developmental biology, agriculture, and disease processes. Data Mining for Systems Biology: Methods and Protocols, surveys and demonstrates the science and technology of converting an unprecedented data deluge to new knowledge and biological insight. The volume is organized around two overlapping themes, network inference and functional inference. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Data Mining for Systems Biology: Methods and Protocols also seeks to aid researchers in the further development of databases, mining and visualization systems that are central to the paradigm altering discoveries being made with increasing frequency.
Автор: Has Cristina, Sitaru Cassian Название: Molecular Dermatology: Methods and Protocols ISBN: 1493959778 ISBN-13(EAN): 9781493959778 Издательство: Springer Рейтинг: Цена: 20263.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: The sustained skin research efforts over the past decades has led to the accumulation of a significant collection of information on skin structure and physiology as well as on the pathogenesis of cutaneous diseases. In Molecular Dermatology: Methods and Protocols, leading experts in the field provide a collection of state-of-the-art reliable protocols covering a wide spectrum of techniques and experimental models, specific molecular assays and disease models, as well as overviews of diagnostic and research areas relevant to molecular dermatology. As a volume in the highly successful Methods in Molecular Biology™ series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Molecular Dermatology: Methods and Protocols emphasizes the vital importance of skin research and collects the methodologies necessary to aid scientists in moving forward in this valuable field.
1. Introduction to Hair-Follicle-Associated Pluripotent (HAP) Stem Cells Robert M. Hoffman 2. Nestin-Based Reporter Transgenic Mouse Lines John Mignone, Natalia Peunova, and Grigori Enikolopov 3. Discovery of HAP Stem Cells Lingna Li and Robert M. Hoffman 4. Peripheral-Nerve and Spinal-Cord Regeneration in Mice Using Hair Follicle-Associated-Pluripotent (HAP) Stem Cells Yasuyuki Amoh, Kensei Katsuoka, and Robert M. Hoffman 5. Construction of Tissue Engineered Nerve Conduits Seeded with Neurons Derived from Hair Follicle Neural Crest Stem Cells Fang Liu, Haiyan Lin, and Chuansen Zhang 6.Nestin-Expressing Hair Follicle-Associated Pluripotent (HAP) Stem Cells Promote Whisker Sensory-Nerve Growth in Long-Term 3D-Gelfoam Histoculture Sumiyuki Mii, Jennifer Duong, Yasunori Tome, Aisada Uchugonova, Fang Liu, Yasuyuki Amoh, Norimitsu Saito, Kensei Katsuoka, and Robert M. Hoffman 7. Isolation and Culture of Neural Crest Stem Cells from Human Hair Follicles Ruifeng Yang and Xiaowei Xu
8. Isolation of Mouse Hair Follicle Bulge Stem Cells and their Functional Analysis in a Reconstitution Assay Ying Zheng, Jen-Chih Hsieh, Julia Escandon, and George Cotsarelis 9.Hair Follicle Regeneration by Transplantation of a Bioengineered Hair Follicle Germ Katsunari Tezuka, Koh-ei Toyoshima, and Takashi Tsuji 10. Hair Induction by Cultured Mesenchymal Cells Using Sphere Formation Kazuo Kishi and Ruka Hayashi 11. Stereological Quantification of Cell Cycle Kinetics and Mobilization of Epithelial Stem Cells during Wound Healing Eduardo Martнnez-Martнnez, Eileen Uribe-Querol, Claudio I. Galvбn-Hernбndez, and Gabriel Gutiйrrez-Ospina 12. Culture of Dermal Papilla Cells from Ovine Wool Follicles: An In Vitro Model for Papilla Size-Determination Nicholas W. Rufaut, Allan J. Nixon, and Rodney D. Sinclair 13. Isolation and Fluorescence Activated Cell-Sorting of Mouse Keratinocytes Expressing β-Galactosidase Maria Kasper, Rune Toftgеrd, and Viljar Jaks 14. Ectopic Hair Growth from Transplanted Whisker Follicles on the Spinal Cord of Mice Wenluo Cao, Fang Liu, Yasuyuki Amoh, and Robert M. Hoffman
15. Long-Term Gelfoam(R) Histoculture of Hair-Shaft-Producing Mouse Whisker Follicles Wenluo Cao, Fang Liu, Yasuyuki Amoh, and Robert M. Hoffman 16. From Hair to Heart: Hair Follicle-Associated-Pluripotent (HAP) Stem Cells Differentiate to Beating Cardiac Muscle Cells Masateru Yashiro, Sumiyuki Mii, Ryoichi Aki, Yuko Hamada, Nobuko Arakawa, Katsumasa Kawahara, Robert M. Hoffman, and Yasuyuki Amoh 17.Highly Efficient Neural Differentiation of CD34 Positive Hair Follicle-Associated Pluripotent (HAP) Stem Cells Induced by Retinoic Acid and Serum Free Medium Mohsen Sagha and Nowruz Najafzadeh 18. Cryopreservation of Intact Hair Follicle Maintains Pluripotency of Nestin-Expressing (HAP) Stem Cells Satoshi Kajiura, Sumiyuki Mii, Ryoichi Aki, Yuko Hamada, Nobuko Arakawa, Katsumasa Kawahara, Lingna Li, Kensei Katsuoka, Robert M. Hoffman, and Yasuyuki Amoh
Автор: Shackleton Sue, Collas Philippe, Schirmer Eric C. Название: The Nuclear Envelope: Methods and Protocols ISBN: 1493980688 ISBN-13(EAN): 9781493980680 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume provides a wide rangeof protocols used in studying the nuclear envelope, with special attention tothe experimental adjustments that may be required to successfully investigatethis complex organelle in cells from various organisms.
Автор: Duque Paula Название: Environmental Responses in Plants: Methods and Protocols ISBN: 1493980297 ISBN-13(EAN): 9781493980291 Издательство: Springer Рейтинг: Цена: 16070.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
PaPart I Tropisms
1.11. Hydrotropism: Analysis of the Root Response to a Moisture Gradient Regina Antoni, Daniela Dietrich, Malcolm J. Bennett, and Pedro L. Rodriguez
2. Assessing Gravitropic Responses in Arabidopsis Richard Barker, Benjamin Cox, Logan Silber, Arah Sangari, Amir Assadi, and Patrick Masson
3. Physiological Analysis of Phototropic Responses in Arabidopsis Mathias Zeidler
4. Automatic Chloroplast Movement Analysis Henrik Johansson and Mathias Zeidler
5. Micro
scopic and Biochemical Visualization of Auxins in Plant Tissues Joshua J. Blakeslee and Angus S. Murphy
6. Immunolocalization of PIN and ABCB Transporters in Plants Nicola Carraro and Wendy A. Peer
Part II Photoperiodism and Circasian Rhythms
7.Analysis of Circadian Leaf Movements Niels A. Mьller and Josй M. Jimйmez-Gуmez
8. Sample Preparation of Arabidopsis Thaliana Shoot Apices for Expression Studies of Photoperiod-Induced Genes Fernando Andrйs, Stefano Torti, Coral Vincent, and George Coupland
9. A Luciferase-Based Assay to Test Whether Gene Expression Responses to Environmental Inputs are
Temporally Restricted by the Circadian Clock Amaury de Montaigu, Markus C. Berns, and Geroge Coupland
10. Identification of Arabidopsis Transcriptional Regulators by Yeast One-Hyrbid Screens Using a Transcription Factor ORFeome Ghislain Breton, Steve A. Kay, and Josй L. Pruneda-Paz
11. Monitoring Alternative Splicing Changes in Arabidopsis Circadian Clock Genes Craig G. Simpson, John Fuller, Cristiane P. G. Calixto, Jim McNicol, Clare Booth, John W. S. Brown, and Dorothee Staiger
12. Assessing the Impact of Photosynthetic Sugars in the Arabidopsis Circadian Clock Michael J. Haydon and Alex A. R. Webb
13. Assessing Protein Stability Under Different Light and Circadian Conditions Takatoshi Kiba and Rossana Henriques
Part IIIAbiotic Stress Responses
14. Screening for Abiotic Stress Tolerance in Rice: Salt, Cold, and Drought Diego M. Almeida, M. Cecнlia Almadanim, Tiago Louren
зo, Isabel A. Abreu, Nelson J. M. Saibo, and M. Margarida Oliveira
15. Basic Techniques to Assess Seed Germination Responses to Abiotic Stress in Arabidopsis Thaliana Urszula Piskurewicz and Luis Lopez-Molina
16. Assessing Tolerance to Heavy-Metal Stress in Arabidopsis Thaliana Seedings Estelle Remy and Paula Duque
17. Assessing Drought Responses Using Thermals Infrared Imaging Ankush Prashar and Hamlyn G. Jones
18. Generating Targeted Gene Knockout Lines in Physcomotrella Patens to Study Evolution of Stress- Responsive Mechanisms Monika Maronova and Maria Kalyna
19. Screening Stress Tolerance Traits in Arabidopsis Cell Cultures Imma P. Salamу, Bogбta Boros, and L
бszlу Szabados
20. Using Arabidopsis Protoplasts to Study Cellular responses to Environmental Stress Ana Confraria and Elena Baena-Gonzбlez
21. Construction of Artificial miRNAs to Prevent Drought Stress in Solanum Tuberosum Anna Wyrzykowska, Marcin Pieczyński, and Zofia Szweykowska-Kuli
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