The Nuclear Envelope: Methods and Protocols, Shackleton Sue, Collas Philippe, Schirmer Eric C.
Автор: Zachariou Michael Название: Affinity Chromatography / Methods and Protocols ISBN: 1588296598 ISBN-13(EAN): 9781588296597 Издательство: Springer Рейтинг: Цена: 2000.00 р. Наличие на складе: Есть (2 шт.) Описание: Thirty-eight years after its introduction, affinity chromatography remains a key tool in th armory of separation techniques available to separation and interaction scientists. Expanded and updated from the first edition, this second edition volume aims to provide the beginner with the practical knowledge needed to develop affinity separations suitable for a variety of applications relevant to the post-genomic era.
Автор: Didenko Vladimir V. Название: In Situ Detection of DNA Damage / Methods and Protocols ISBN: 0896039528 ISBN-13(EAN): 9780896039520 Издательство: Springer Рейтинг: Цена: 5809.00 р. Наличие на складе: Есть (4 шт.) Описание: In In Situ Detection of DNA Damage: Methods and Protocols, Vladimir Didenko and a panel of experts describe all the major in situ techniques for studying DNA damage and apoptosis, and how approaches originally designed to label apoptotic cells can be used for DNA damage analysis (and vice versa). The readily reproducible techniques presented here include all those designed to detect specific single- and double-stranded DNA breaks in tissue sections while using polymerases, ligases, nucleases, and kinases. There are also novel instrumental techniques that use ultrasound for in vivo and in situ detection of apoptotic DNA damage, and in situ applications of flow and laser-scanning cytometry for analysis of DNA strand breaks and apoptosis. State-of-the-art and highly practical, In Situ Detection of DNA Damage: Methods and Protocols presents for the first time a complete set of enzymatic methods for determining DNA breaks, and will greatly facilitate the work of all those studying the in situ detection of DNA damage and the labeling of apoptotic cells.
Автор: Haqqi Tariq M., Lefebvre Vйronique Название: Chondrocytes: Methods and Protocols ISBN: 1071611186 ISBN-13(EAN): 9781071611180 Издательство: Springer Цена: 16769.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This detailed book collects a variety of methods from investigators at the forefront of chondrocyte biology and pathology research that they have tailored for their research projects in order to tackle the unique properties and challenges of the chondrocyte and those of its tissue matrix.
Автор: Amarnath Shoba Название: Innate Lymphoid Cells: Methods and Protocols ISBN: 107160337X ISBN-13(EAN): 9781071603376 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Поставка под заказ.
Автор: Chang Paul, Ohi Ryoma Название: The Mitotic Spindle: Methods and Protocols ISBN: 1493980726 ISBN-13(EAN): 9781493980727 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
Part I Methods Focused on Cytology 1: Using Fluorescence Microscopy to Study MitosisSai K. Balchand, Barbara J. Mann, and Patricia Wadsworth 2: Using Photoactivatable GFP to Study Microtubule Dynamics and Chromosome Segregation Bin He and Daniela Cimini
Part II Methods Focused on Microtubules and the Mitotic Spindle
3: Purification and Fluorescent Labeling of Tubulin from Xenopus Laevis Egg Extracts Aaron C. Groen and Timothy J. Mitchison 4: Measuring the Effects of Microtubule-Associated Proteins on Microtubule Dynamics In Vitro Marija Zanic
5: Imaging and Quantifying the Dynamics of γ-Tubulin at Microtubule Minus Ends in Mitotic Spindles Nicolas Lecland and Jens Lьders 6: Visualizing and Analyzing Branching Microtubule Nucleation Using Meiotic Xenopus Egg Extracts and TIRF Microscopy Matthew King and Sabine Petry 7: Encapsulation of Xenopus Egg and Embryo Extract Spindle Assembly Reactions in Synthetic Cell-Like Compartments with Tunable Size Matthew C. Good Part III Methods Focused on Kinetochores and the Kinetochore-Microtubule Interface 8: In Vitro Kinetochore Assembly Matthew D. D. Miell and Aaron F. Straight 9: Biochemical and Structural Analysis of Kinetochore Histone-Fold Complexes Tatsuya Nishino and Tatsuo Fukagawa 10: Measuring Kinetochore-Microtubule Attachment Stability in Cultured Cells Keith F. DeLuca, Jacob A. Herman, and Jennifer G. DeLuca 11: Studying Kinetochores In Vivo Using FLIM-FRET Tae Yeon Yoo and Daniel J. Needleman Section IV Methods Focused on the Spindle Pole 12: Purification of Fluorescently Labeled Saccharomyces Cerevisiae Spindle Pole Bodies Kimberly K. Fong, Beth Graczyk, and Trisha N. Davis 13: A Cell-Free System for Real-Time Analyses of Centriole Disengagement and Centriole to Centrosome Conversion Rajesh Kumar Soni and Meng-Fu Bryan Tsou 14: Assays to Study Mitotic Centrosome and Spindle Pole Assembly and Regulation Vladimir Joukov, Johannes C. Walter, and Arcangela De Nicolo Part V Methods Focused on the Cellular Functions of Microtubule Motor Proteins 15: Analyzing Spindle Positioning Dynamics in Cultured Cells Tomomi Kiyomitsu 16: Quantification of Mitotic Chromosome Alignment Cindy Fonseca and Jason Stumpff 17: Imaging Mitosis in the Moss Physcomitrella Patens Moй Yamada, Tomohiro Miki, and Gohta Goshima 18: Small Molecule Approach to Study the Function of Mitotic Kinesins Naowras Al-Obaidi, Johanna Kastl, and Thomas U. Mayer Part VI Novel Approaches to Study Spindle Function and Regulation 19: Identification and Characterization of Mitotic Spindle-Localized Transcripts Amy B. Emerman, Ashwini Jambhekar, and Michael D. Blower 20: Probing Mitosis by Manipulating the Interactions of Mitotic Regulator Proteins Using Rapamycin-Inducible Dimerization Edward R. Ballister and Michael A. Lampson 21: Studying Kinetochore Kinases Adrian T. Saurin and Geert J.P.L. Kops 22: Engineering and Functional Analysis of Mitotic Kinases through Chemical Genetics Mathew J.K. Jones and Prasad V. Jallepalli Part VII The Mitotic Spindle and Cancer 23: Using Cell Culture Models of Centrosome Amplification to Study Centrosome Clustering in Cancer Mijung Kwon
Автор: Feng Xin-Hua, Xu Pinglong, Lin Xia Название: Tgf-β Signaling: Methods and Protocols ISBN: 1493949934 ISBN-13(EAN): 9781493949939 Издательство: Springer Рейтинг: Цена: 16070.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Chapters provide basic introduction of TGF-ss signaling from the cell surface to the nucleus, methods and techniques for the investigation of TGF-ss signaling mechanism including receptors, Smads, intracellular kinases, microRNA, epigenetic regulation, post-translational regulations, non-Smad pathway;
Автор: Eric C. Schirmer; Jose I. de las Heras Название: Cancer Biology and the Nuclear Envelope ISBN: 1489980318 ISBN-13(EAN): 9781489980311 Издательство: Springer Рейтинг: Цена: 36570.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: "Nuclear envelope (NE) defects have been linked to cancer biology since the mid-1800s, but it was not until the last few years that we have begun to understand these historical links and to realize that there are myriad ways that the NE impacts on tumorigenesis.
Автор: Turksen Kursad Название: Stem Cell Heterogeneity: Methods and Protocols ISBN: 1493982346 ISBN-13(EAN): 9781493982349 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Given the variety of studies and data that have suggested the existence of heterogeneous populations or subpopulations of stem cells, this detailed volume examines different aspects of stem cell heterogeneity.
Автор: Ram K. Mishra; Glen B. Baker; Alan A. Boulton Название: G Protein Methods and Protocols ISBN: 1489942904 ISBN-13(EAN): 9781489942906 Издательство: Springer Рейтинг: Цена: 22203.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Автор: Weichhart Thomas Название: Mtor: Methods and Protocols ISBN: 1493962094 ISBN-13(EAN): 9781493962099 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: In mTOR: Methods and Protocols expert researchers in the field detail many of the methods which are now commonly used to study mTOR. These include methods and techniques used for the study of the mTOR pathway and potential therapeutic applications of mTOR inhibitors such as, immunosuppressive and anticancer agents.
Автор: Sue Shackleton; Philippe Collas; Eric C. Schirmer Название: The Nuclear Envelope ISBN: 1493935283 ISBN-13(EAN): 9781493935284 Издательство: Springer Рейтинг: Цена: 22359.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume provides a wide rangeof protocols used in studying the nuclear envelope, with special attention tothe experimental adjustments that may be required to successfully investigatethis complex organelle in cells from various organisms.
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