Viruses as Therapeutics: Methods and Protocols, Lucas Alexandra R.

Автор: Merten
Название: Viral Vectors for Gene Therapy
ISBN: 161779094X ISBN-13(EAN): 9781617790942
Издательство: Springer
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Цена: 22359.00 р.
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Описание: The huge potential for gene therapy to cure a wide range of diseases has led to high expectations and a great increase in research efforts in this area, particularly in the study of delivery via viral vectors, widely considered to be more efficient than DNA transfection.  In Viral Vectors for Gene Therapy: Methods and Protocols, experts in the field present a collection of their knowledge and experience featuring methodologies that involve virus production, transferring protocols, and evaluating the efficacy of gene products. While thoroughly covering the most popular viral vector systems of adenovirus, retrovirus, and adeno-associated virus, this detailed volume also explores less common viral vector systems such as baculovirus, herpes virus, and measles virus, the growing interest in which is creating a considerable demand for large scale manufacturing and purification procedures.  Written in the highly successful Methods in Molecular Biology™ series format, many chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, Viral Vectors for Gene Therapy: Methods and Protocols provides basic principles accessible to scientists from a wide variety of backgrounds for the development of gene therapy viral products that are safe and effective.

Автор: Cavanagh
Название: SARS- and Other Coronaviruses
ISBN: 1617378151 ISBN-13(EAN): 9781617378157
Издательство: Springer
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Цена: 19564.00 р.
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Описание: In 2003, the word "coronavirus" spread across the globe, somewhat further than the virus that sparked the panic. In this book, expert researchers examine these devastating viruses through 23 state-of-the-art, widely applicable protocols with minute detail.

Автор: Lion
Название: Human Fungal Pathogen Identification
ISBN: 1493965131 ISBN-13(EAN): 9781493965137
Издательство: Springer
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Цена: 20962.00 р.
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Описание: This detailed volume presents timely and authoritative content offering a comprehensive overview of the current state of the art in fungal diagnostics. Moreover, it addresses on-going developments expected to provide a basis for targeted treatment strategies resulting in improved outcome of invasive mycoses. The knowledge of host-related predisposing factors and stratified treatment options facilitating timely onset of adequate antifungal therapy are critical for successful clinical management and outcome of invasive fungal disease (IFD), requiring not only rapid diagnosis of a fungal infection and identification of the causative species, but also assessment of pathogen/host factors related to pathogenicity, susceptibility, and response to treatment. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.

Название: Poliovirus
ISBN: 1493932918 ISBN-13(EAN): 9781493932917
Издательство: Springer
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Цена: 19564.00 р.
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Описание: Poliovirus

Автор: Basha, Mahin
Название: Viruses as Therapeutics
ISBN: 1071610147 ISBN-13(EAN): 9781071610145
Издательство: Springer
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Цена: 18167.00 р.
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Описание: Beginning with methods for the development and study of viruses, the book continues with current techniques and approaches used to develop new treatments from virus-derived vaccines to vectors and proteins, such as a range of models and methods designed to measure therapeutic efficacy of virus-derived biologics and therapeutic proteins.

Автор: Paul M. Lieberman
Название: DNA Viruses
ISBN: 1627038108 ISBN-13(EAN): 9781627038102
Издательство: Springer
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Цена: 23757.00 р.
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Описание: Wide-ranging and highly practical, DNA Viruses: Methods and Protocols will stimulate new directions in virology research with its novel strategies for engineering viral vectors in gene therapy, and its advanced approaches for detecting viruses in human disease.

Автор: Ichiro Uyeda; Chikara Masuta
Название: Plant Virology Protocols
ISBN: 1493955403 ISBN-13(EAN): 9781493955404
Издательство: Springer
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Цена: 15372.00 р.
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Описание: Plant Virology Protocols: New Approaches to Detect Viruses and Host Responses addresses recent developments in genome analyses and cytological technologies being used today to learn more about plant virology.

Автор: Bhat
Название: Characterization of Plant Viruses
ISBN: 1071603337 ISBN-13(EAN): 9781071603338
Издательство: Springer
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Цена: 18167.00 р.
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Описание: This book provides detailed information on methodologies used in biological, serological and nucleic acid based assays for the detection, diagnosis and management of plant viruses. The content is divided into six main parts, the first of which presents techniques used in the biological characterization and transmission of viruses, while Part II covers purification and techniques concerning the physico-chemical properties of viruses. In turn, Part III focuses on in vitro expression, production of polyclonal and monoclonal antibodies; and on various serological assays including precipitin tests, ELISA, blot immunoassays, immunosorbent electron microscopy and lateral flow immunoassays. Part IV addresses the isolation of DNA and RNA from plants and nucleic acid based assays such as dot-blot, polymerase chain reaction, real-time PCR, loop-mediated isothermal amplification, rolling circle amplification, recombinase polymerase amplification, and next-generation sequencing approaches. Part V discusses cloning, sequencing, sequence analysis and the production of infectious clones, while the last part (Part VI) provides biotechnological approaches for the management of viruses. Given its scope, the book will be a valuable resource for every laboratory, student and teacher, and for everyone interested in plant virology, plant pathology, plant biology and molecular biology, offering them a practical manual on various aspects of plant viruses.

Название: Bacterial viruses
ISBN: 1913652513 ISBN-13(EAN): 9781913652517
Издательство: Неизвестно
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Цена: 42029.00 р.
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Автор: Stephenson
Название: Diagnostic Virology Protocols
ISBN: 1607618168 ISBN-13(EAN): 9781607618164
Издательство: Springer
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Цена: 23757.00 р.
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Описание: Recent outbreaks of swine influenza and avian influenza, along with the remaining and in some cases expanding threats from HIV, dengue virus, and the viruses causing hepatitis, have reinforced the need for rapid, accurate and cost-effective diagnosis of viral disease. Diagnostic Virology Protocols, Second Edition brings the field fully up-to-date with a focus on protocols involving nucleic acid detection, most often through some form of the polymerase chain reaction (PCR). The expert contributors also delve into the key technology of robotics as well as future prospects, such as further refined point-of-care testing and the increasing importance of mathematical modelling. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Diagnostic Virology Protocols, Second Edition captures the dramatic changes in the virus diagnostic laboratory in order to better prepare scientists to combat the inevitable threats to public health from future and present infectious diseases.

Автор: Sugrue
Название: Glycovirology Protocols
ISBN: 1617376647 ISBN-13(EAN): 9781617376641
Издательство: Springer
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Цена: 18167.00 р.
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Описание: This volume reviews the increasing importance of glycosylation to the field of virology, as well as virus replication. The chapters provide an overview of glycosylation in relation to virus infection, and the generic techniques that are used to analyze and characterize glycoproteins.

Автор: Davy
Название: Human Papillomaviruses
ISBN: 1617375268 ISBN-13(EAN): 9781617375262
Издательство: Springer
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Цена: 29209.00 р.
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Описание: Features leading basic researchers and clinical scientists who describe in detail a wide variety of established and advanced techniques they have developed to study the lifecycle and biological properties of the human papillomavirus. In this title, the protocols follow the successful "Methods in Molecular Medicinea" series format.