Nlr Proteins: Methods and Protocols, Di Virgilio Francesco, Pelegrнn Pablo
Название: Nlr proteins ISBN: 149393564X ISBN-13(EAN): 9781493935642 Издательство: Springer Рейтинг: Цена: 13275.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Chapters focus on of innate immune receptors, "atypical"inflammasomes, biochemical and novel bioluminescencetechniques for the measurement of IL-1b,bioluminescent probe, biochemical and microscopytechniques, techniques to measure caspase-1 activation, cellfree systems for the study of inflammasome function, and inflammasomeactivation.
Автор: Aguilar Marie-Isabel Название: HPLC of Peptides and Proteins / Methods and Protocols ISBN: 0896039773 ISBN-13(EAN): 9780896039773 Издательство: Springer Рейтинг: Цена: 23757.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Hands-on experts from academia and industry comprehensively describe how to successfully perform all the critical HPLC techniques needed for the analysis of peptides and proteins. The methods range from commonly used techniques to those for capillary to large-scale preparative isolation. The authors have also presented a number of specific applications as case studies to illustrate the analytical approaches to a particular separation or assay challenge, with examples drawn from contemporary fields in biochemistry and biotechnology.
Автор: Smales C. Mark, James David C. Название: Therapeutic Proteins / Methods and Protocols ISBN: 1588293904 ISBN-13(EAN): 9781588293909 Издательство: Springer Рейтинг: Цена: 27950.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: World-class researchers share their innovative and proven techniques for the production of therapeutic proteins downstream of the discovery stage. The methods use a variety of sources for producing therapeutic proteins, including bacterial and yeast expression systems, and insect and mammalian cells, and then cover the purification of the resulting protein using both state-of-the-art and traditional methods, such as those sourced from plasma. Additional chapters offer methods for the characterization of therapeutic proteins throughout the production process, along with methods and strategies for viral inactivation and protein formulation. The protocols follow the successful Methods in Molecular Biologyв„ў series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Автор: Constance Oliver; Maria Celia Jamur (Eds.) Название: Immunocytochemical methods and protocols ISBN: 1588294633 ISBN-13(EAN): 9781588294630 Издательство: Springer Рейтинг: Цена: 9084.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This third edition reflects the rapid evolution of immunocytochemistry in the post-genomic era. Expert researchers explore the latest methods, now widely used in situ to identify components of cells and tissues in both normal and pathological conditions.
Автор: Simpson Richard J., Greening David W. Название: Serum/Plasma Proteomics: Methods and Protocols ISBN: 1493958011 ISBN-13(EAN): 9781493958016 Издательство: Springer Рейтинг: Цена: 20263.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Blood science has become a cornerstone of multiple disciplines, including clinical chemistry, disease diagnosis, and therapeutic monitoring. Over the past decade, we have witnessed the advent of increasingly powerful proteomics technologies that allow greater fundamental insights into the blood proteome. These technological improvements have, in part, fuelled the quest for the discovery of novel blood-based biomarkers of disease. Serum/Plasma Proteomics: Methods and Protocols is a comprehensive resource of protocols for areas, pre-analytical through to analytical, of plasma and serum proteomics. Divided into five convenient sections, this detailed volume covers fractionation strategies for in-depth blood proteome analysis, defined procedures for blood collection, handling and storage, detailed protocols for performing both antibody-based and non-antibody based quantitative assays, proteome analysis of blood cell compartments, circulating nanomebraneous vesicles and blood-related fluids, and finally data management, statistical design, and bioinformatic challenges. This book, contributed to by leading experts in the field, provides a valuable foundation for the development and application of blood-based proteomics. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Serum/Plasma Proteomics: Methods and Protocols, with its well-honed methodologies, seeks to serve both professionals and investigators new to the field in an effort to further our knowledge of this fundamental science.
Автор: Oliver Название: Immunocytochemical Methods and Protocols ISBN: 1617796824 ISBN-13(EAN): 9781617796821 Издательство: Springer Рейтинг: Цена: 18167.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This third edition reflects the rapid evolution of immunocytochemistry in the post-genomic era. Expert researchers explore the latest methods, now widely used in situ to identify components of cells and tissues in both normal and pathological conditions.
Автор: Claire Lugnier Название: Phosphodiesterase Methods and Protocols ISBN: 1617374873 ISBN-13(EAN): 9781617374876 Издательство: Springer Рейтинг: Цена: 20263.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Study of Cyclic Adenosine Monophosphate Microdomains in Cells Marco Mongillo, Anna Terrin, Sandrine Evellin, Valentina Lissandron, and Manuela Zaccolo High-Resolution Measurements of Cyclic Adenosine Monophosphate Signals in 3D Microdomains Jeffrey W. Karpen and Thomas C. Rich Cygnets: In Vivo Characterization of Novel cGMP Indicators and In Vivo Imaging of Intracellular cGMP Akira Honda, Carolyn L. Sawyer, Sharon M. Cawley, and Wolfgang R. G. Dostmann High-Throughput Screening of Phosphodiesterase Activity in Living Cells Thomas C. Rich and Jeffrey W. Karpen Assessment of Phosphodiesterase Isozyme Contribution in Cell and Tissue Extracts Thйrиse Keravis, Rima Thaseldar-Roumiй, and Claire Lugnier Localization of the Cyclic Guanosine 39,59-Monophosphate- Hydrolyzing Phosphodiesterase Type 9 in Rat Brain by Nonradioactive In Situ Hybridization Wilma C. G. van Staveren and Marjanne Markerink-van Ittersum Determination of Ca2+/Calmodulin-Stimulated Phosphodiesterase Activity in Intact Cells Chen Yan Adenovirus-Mediated Overexpression of Murine Cyclic Nucleotide Phosphodiesterase 3B Faiyaz Ahmad, Linda Hдrndahl, Yan Tang, Lena Stenson Holst, and Vincent C. Manganiello Identification of Promoter Elements in the 5'-Flanking Region of Murine Cyclic Nucleotide Phosphodiesterase 3B Gene Hanguan Liu, Jing Rong Tang, Eva Degerman, and Vincent C. Manganiello Purification of PDE6 Isozymes From Mammalian Retina Dana C. Pentia, Suzanne Hosier, Rachel A. Collupy, Beverly A. Valeriani, and Rick H. Cote Cyclic Guanosine 59-Monophosphate Binding to Regulatory GAF Domains of Photoreceptor Phosphodiesterase Rick H. Cote Renaturation of the Catalytic Domain of PDE4A Expressed in Escherichia coli as Inclusion Bodies Wito Richter, Thomas Hermsdorf, and Dietrich Dettmer Determining the SubunitStructure of Phosphodiesterases Using Gel Filtration and Sucrose Density Gradient Centrifugation Wito Richter Crystallization of Cyclic Nucleotide Phosphodiesterases Hengming Ke, Qing Huai, and Robert X. Xu Generation of PDE4 Knockout Mice by Gene Targeting S.-L. Catherine Jin, Anne M. Latour, and Marco Conti Immunoprecipitation of PDE2 Phosphorylated and Inactivated by an Associated Protein Kinase J. Kelley Bentley Investigation of Extracellular Signal-Regulated Kinase 2 Mitogen-Activated Protein Kinase Phosphorylation and Regulation of Activity of PDE4 Cyclic Adenosine Monophosphate-Specific Phosphodiesterases Elaine V. Hill, Miles D. Houslay, and George S. Baillie Radiolabeled Ligand Binding to the Catalytic or Allosteric Sites of PDE5 and PDE11 James L. Weeks II, Mitsi A. Blount, Alfreda Beasley, Roya Zoraghi, Melissa K. Thomas, Konjeti Raja Sekhar, Jackie D. Corbin, and Sharron H. Francis Analysis of Dimerization Determinants of the PDE6 Catalytic Subunits Khakim G. Muradov, Kimberly K. Boyd, and Nikolai O. Artemyev Interaction Between Catalytic and Inhibitory Subunits of PDE6 Nikolai O. Artemyev Purification, Reconstitution on Lipid Vesicles, and Assays of PDE6 and Its Activator G Protein, Transducin Theodore G. Wensel, Feng He, and Justine A. Malinski Index
Автор: Bhattacharya Sanjoy K. Название: Lipidomics: Methods and Protocols ISBN: 1493983628 ISBN-13(EAN): 9781493983629 Издательство: Springer Рейтинг: Цена: 19564.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
Preface... Table of Contents... Contributing Authors...
1. Lipid Sample Preparation for Biomedical Research Ravin Sajnani and Katyayini Aribindi 2. Lipid Extraction Techniques for Stable Isotope Analysis and Ecological Assays Kyle H. Elliot, James D. Roth, and Kevin Crook 3. Isolation of Lipid Raft Proteins from CD133+ Cancer Stem Cells Vineet K. Gupta and Sulagna Banerjee 4. Isolation of Neuronal Synaptic Membranes by Sucrose Gradient Centrifugation Blake E. Hopiavuori, Dustin E. Masser, Joseph L. Wilkerson, Richard S. Brush, Nawajes A. Mandal, Robert E. Anderson, and Willard M. Freeman 5. Sample Preparation and Analysis for Imaging Mass Spectrometry Genea Edwards, Annia Mesa, Robert I. Vazquez-Padron, Paul Kowalski, and Sanjoy K. Bhattacharya 6. Direct Measurement of Free and Esterified Cholesterol Mass in Differentiated Human Podocytes: A TLC and Enzymatic Assay Based Method Christopher E. Pedigo, Sandra M. Merscher, and Alessia Fornoni 7. High-Performance Chromatographic Separation of Cerebrosides Renaud Sicard and Ralf Landgraf 8. Lipid Identification by Untargeted Tandem Mass Spectrometry Coupled with Ultra-High-Pressure Liquid Chromatography Gabriel B. Gugiu 9. Utility of Moderate and High Resolution Mass Spectrometry for Class-Specific Lipid Identification and Quantification Maria del Carmen Piqueras 10. A Robust Lipidomics Workflow for Mammalian Cells, Plasma, and Tissue using Liquid-Chromatography High-Resolution Tandem Mass Spectrometry Candice Z. Ulmer, Rainey Patterson, Jeremy Koelmel, Timothy J. Garrett, and Richard A. Yost 11. Combined Use of MALDI-TOF Mass Spectrometry and 31PNMR Spectrometry for Analysis of Phospholipids Jenny Schrцter, Yulia Popkova, Rosmarie Sь , and Jьrgen Schiller 12. Global Monitoring of Mammalian Lipidome by Quantitative Shotgun Lipodomics Inger dum Nielsen, Kenji Maeda, and Mesut Bilgin13. Bioinformatics Pertinent to Lipid Analysis in Biological Samples Justin Ma, Ulises Arbelo, Yenifer Guerra, Katyayini Aribindi, Sanjoy K. Bhattacharya, and Daniel Pelaez 14. LC-MS-Based Lipidomics and Automated Identification of Lipids using the LipidBlast In-Silico MS/MS Library Tomas Cajka and Oliver Fiehn 15. Single-Step Capture and Targeted Metabolomics of Alkyl-Quinolones in Outer Membrane Vesicles of Pseudomonas aeruginosa Pallavi Lahiri and Dipankar Ghosh 16. Analysis of Fatty Acid and Cholesterol Content from Detergent-Resistant and Detergent-Free Membrane Microdomains Mark E. McClellan and Michael H. Elliott 17. Computational Functional Analysis of Lipid Metabolic Enzymes Carolina Bagnato, Arjen Ten Have, Marнa B. Prados, and Marнa B. Beligni 18. Isoprenylation of Monomeric GTPases in Human Trabecular Meshwork Cells Evan B. Stubbs, Jr. 19. Purification and Validation of Lipid Transfer Proteins Matti A. Kjellberg, Anders P.E. Backman, Anna Mцuts, and Peter Mattjus 20. Incorporation of Artificial Lipid-Anchored Proteins into Cultured Mammalian Cells Rania Leventis and John R. Silvius 21. Sonication-Based Basic Protocol for Liposome Synthesis Roberto Mendez and Santanu Banerjee 22. On Electrochemical Methods for Determination of Protein-Lipid Interaction Zhiping Hu and Yanli Mao 23. Angiogenesis Model of Cornea to Understand the Role of Sphingosine 1-Phosphate Joseph L. Wilkerson and Nawajes A. Mandal
Автор: Jimenez Gerardo Название: Erk Signaling: Methods and Protocols ISBN: 1493981951 ISBN-13(EAN): 9781493981953 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
1. How Genetics has Helped Piece Together the MAPK Signaling Pathway Dariel Ashton-Beaucage and Marc Therrien 2. In Vitro Enzyme Kinetics Analysis of EGFR Zhihong Wang and Christine Candelora
3. High-Throughput Analysis of Mammalian Receptor Tyrosine Kinase Activation in Yeast Cells Nobuo Yoshimoto and Shun'ichi Kuroda 4. Structural Studies of ERK2 Protein Complexes Johannes F. Weijman, Stefan J. Riedl, and Peter D. Mace 5. Isolation and Characterization of Intrinsically Active (MEK-Independent) Mutants of Mpk1/Erk Tal Goshen-Lago, Dganit Melamed, Arie Admon, and David Engelberg 6. Assaying Activation and Subcellular Localization of ERK in Cells and Tissues Carme Caelles, Carles Bayod, and Melisa Morcillo 7. Detection and Functional Analysis of SUMO-Modified MEK Yuji Kubota and Mutsuhiro Takekawa8. Single-Step Affinity Purification of ERK Signaling Complexes Using the Streptavidin-Binding Peptide (SBP) Tag Liu Yang and Alexey Veraksa 9. High-Throughput In Vitro Identification of Direct MAPK/Erk Substrates Rona Grossmanand Ze'ev Paroush 10. Global Identification of ERK Substrates by Phosphoproteomics Based on IMAC and 2D-DIGE Hidetaka Kosako and Kou Motani 11. Analysis of Ras/ERK Compartmentalization by Subcellular Fractionation Lorena Agudo-Ibaсez, Piero Crespo, and Berta Casar 12. Cell-Based Assays to Study ERK Pathway/Caveolin1 Interactions Raffaele Strippoli, Asier Echarri, Miguel Angel del Pozo 13. The Nuclear Translocation of ERK Denise A. Berti and Rony Seger 14. Visualization of RAS/MAPK Signaling In Situ by the Proximity Ligation Assay (PLA) Zijian Tang and Chengkai Dai 15. Measuring ERK Activity Dynamics in Single Living Cells Using FRET Biosensors Yannick Blum, Rafael D. Fritz, Hyunryul Ryu, and Olivier Pertz 16. Quantifying Tensile Force and ERK Phosphorylation on Actin Stress Fibers Hiroaki Hirata, Mukund Gupta, Sri Ram Krishna Vedula, Chwee Teck Lim, Benoit Ladoux, and Masahiro Sokabe 17. Co-Culture Activation of MAP Kinase in Drosophila S2 Cells Josefa Steinhauer 18. Isolation of Mouse Embryonic Stem Cell Lines in the Study of ERK1/2 MAP Kinase Signaling Marc K. Saba-El-Leil, Christophe Frйmin, and Sylvain Meloche 19. 3D Organotypic Culture Model to Study Components of ERK Signaling Athina-Myrto Chioni, Rabia Tayba Bajwa, and Richard Grose 20. Genetic Validation of Cell Proliferation via Ras-Independent Activation of the Raf/Mek/Erk Pathway Carmen G. Lechuga, Lucнa Simуn-Carrasco, Harrys K.C. Jacob, and Matthias Drosten 21. Genome-Wide Analysis of RAS/ERK Signaling Targets Joshua P. Plotnik and Peter C. Hollenhorst 22. Probing Chromatin Modifications in Response to ERK Signaling Ozgur Oksuz and Wee-Wei Tee 23. Analyzing pERK Activation during Planarian Regeneration Susanna Fraguas, Yoshihiko Umesono, Kiyokazu Agata, and FrancescCebriа 24. Discovering Functional ERK Substrates Regulating Caenorhabditis elegans Germline Development Jessica Jie Chen and Swathi Arur 25. Reconstructing ERK Signaling in the Drosophila Embryo from Fixed Images Bomyi Lim, Carmeline J. Dsilva, Ioannis G. Kevrekidis, and Stanislav Y. Shvartsman
Автор: Bhupendra Pushkar Название: Protein Synthesis: Methods and Protocols ISBN: 1774077035 ISBN-13(EAN): 9781774077030 Издательство: Mare Nostrum (Eurospan) Рейтинг: Цена: 22730.00 р. Наличие на складе: Нет в наличии.
Описание: Takes readers through the various ways in which proteins are synthesized and then further used for other applications. The book throws light on chemical reactions taking place in the process, and lays down different kinds of methods and the procedures that are followed in protein synthesis.
Автор: Galea Charles A. Название: Matrix Metalloproteases: Methods and Protocols ISBN: 1493983210 ISBN-13(EAN): 9781493983216 Издательство: Springer Рейтинг: Цена: 16070.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
PART I Expression and Purification of Matrix Metalloproteases
1. Expression and Purification of Matrix Metalloproteinases in Escherichia coli
Krishna K. Singh, Ruchi Jain, Harini Ramanan, and Deepak K. Saini
2. Expression and Purification of a Matrix Metalloprotease Transmembrane Domain in Escherichia coli
Charles A. Galea
3. Heterologous Expression of the Astacin Protease Meprin b in Pichia pastoris
Dagmar Schlenzig and Stephan Schilling
PART II Structural Characterization of Matrix Metalloproteases
4. Structural Studies of Matrix Metalloproteinase by X-Ray Diffraction
Elena Decaneto, Wolfgang Lubitz, and Hideaki Ogata
5. Mapping Lipid Bilayer Recognition Sites of Metalloproteinases and other Prospective Peripheral Membrane Proteins
Tara C. Marcink, Rama K. Koppisetti, Yan G. Fulcher, and Steven R. Van Doren
6. Using Small Angle X-ray Scattering (SAXS) to Characterise the Solution Conformation and Flexibility of Matrix Metalloproteinases (MMPs)
Louise E. Butt, Robert A. Holland, Nikul S. Khunti, Debra L. Quinn, and Andrew R. Pickford
PART III Computational Simulations of Matrix Metalloproteases
7. Molecular Dynamics Studies of Matrix Metalloproteases
Natalia Dнaz and Dimas Suбrez
PART IV Determining Matrix Metalloprotease Substrate Specificity
8. Determining the Substrate Specificity of Matrix Metalloproteases using Fluorogenic Peptide Substrates
Maciej J. Stawikowski, Anna M. Knapinska, and Gregg B. Fields
9. Time-resolved Analysis of Matrix Metalloproteinase Substrates in Complex Samples
Pascal Schlage, Fabian E. Egli, and Ulrich auf dem Keller
10. Identification of Protease Cleavage Sites by Charge-Based Enrichment of Protein N-termini
Zon W. Lai and Oliver Schilling
11. Mapping the Substrate Recognition Landscapes of Metalloproteases using Comprehensive Mutagenesis
Colin A. Kretz
PART V Detection of Matrix Metalloproteases
12. Detection of Matrix Metalloproteinases by Zymography
Rajeev B. Tajhya, Rutvik S. Patel, and Christine Beeton
13. Imaging Matrix Metalloproteases in Spontaneous Colon Tumors: Validation by Correlation with Histopathology
Harvey Hensley, Harry S. Cooper, Wen-Chi L. Chang, and Margie L. Clapper
PART VI Matrix Metalloprotease Inhibitors 14. Virtual High-throughput Screening for Matrix Metalloproteinase Inhibitors Jun Yong Choi, and Rita Fuerst 15. Computational Approaches to Matrix Metalloprotease Drug Design
Tanya Singh, B. Jayaram, and Olayiwola Adedotun Adekoya
16. A Simple, Adaptable Blood-Brain Barrier Cell Model for Screening Matrix Metalloproteinase Inhibitor Functionality
Jennifer S. Myers, Joan Hare, and Qing-Xiang Amy Sang
PART VII Matrix Metalloproteases as Biomarkers
17. Matrix Metalloproteases as Biomarkers of Disease
Fernando Luiz Affonso Fonseca, Beatriz da Costa Aguiar Alves, Ligia Ajaime Azzalis, and Thaнs Moura Gбscon Belardo
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