Protein Synthesis: Methods and Protocols, Bhupendra Pushkar
Автор: Schueler-Furman, Ora;London, Nir Название: Modeling Peptide-Protein Interactions : Methods and Protocols ISBN: 1493967967 ISBN-13(EAN): 9781493967964 Издательство: Springer Рейтинг: Цена: 13974.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume covers an array of techniques available for studying peptide-protein docking and design.
Автор: Kister Alexander E. Название: Protein Supersecondary Structures: Methods and Protocols ISBN: 1493991604 ISBN-13(EAN): 9781493991600 Издательство: Springer Рейтинг: Цена: 20962.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
This second edition volume expands on the previous edition with an update on the latest developments in the field and new techniques used to study secondary and supersecondary structures (SSS) in proteins. Chapters in this book discuss topics such as sequence and structural features of different SSS elements; software used for the automatic annotation of secondary structure elements in proteins; the new developments in secondary and SSS prediction and the modern approaches for energy landscape calculation; protein misfolding and amyloid structural formation; analysis of ‘transformer proteins’; discovery of the structure of the hydrophobic nucleus; and discussions of the main principles of protein structures formation. The contributing authors of the volume are the eminent experts in the field of protein research and bioinformatics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and authoritative, Protein Supersecondary Structures: Methods and Protocols, Second Edition is a valuable resource for researchers who are interested in learning more about the relationship between amino acids sequences and protein structures, the evolution of proteins and the dynamics of protein formation.
Автор: Walls Dermot, Loughran Sinйad T. Название: Protein Chromatography: Methods and Protocols ISBN: 1493961292 ISBN-13(EAN): 9781493961290 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: A prerequisite for elucidating the structure and function of any protein is the prior purification of that protein. This necessity has led to the development of many purification schemes and chromatographic methods for the isolation of native proteins from complex sources. In Protein Chromatography: Methods and Protocols, leading researchers present clear protocol-style chapters that are suitable for newcomers and experts alike. The book opens with vital topics in protein biochemistry, addressing such areas as protein stability and storage, avoiding proteolysis during chromatography, protein quantitation methods including immuno-qPCR, and the contrasting challenges that microfluidics and scale-up production pose to the investigator, and then it segues into key methods involving the generation and purification of recombinant proteins through recombinant antibody production and the tagging of proteins, amongst other means, as well as many variations on classic techniques such as ion-exchange and immunoaffinity chromatography. Written in the highly successful Methods in Molecular Biology™ series format, protocols chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Protein Chromatography: Methods and Protocols will greatly aid scientists in establishing these essential techniques in their own laboratories and furthering our understanding of the many imperative functions of proteins.
Автор: Pompa Andrea, De Marchis Francesca Название: Unconventional Protein Secretion: Methods and Protocols ISBN: 1493981447 ISBN-13(EAN): 9781493981441 Издательство: Springer Рейтинг: Цена: 6986.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
Part I: Background
1. ER to Golgi Dependent Protein Secretion: The Conventional Pathway
Corrado Viotti
2. Unconventional Protein Secretion in Animal Cells
Fanny Ng and Bor Luen Tang
3. Unconventional Protein Secretion in Plants
Destiny J. Davis, Byung-Ho Kang, Angelo S. Heringer, Thomas E. Wilkop, and Georgia Drakakaki
Part II: UPS Contemplates Multidisciplinary Approaches for Plants
4. Chemical Secretory Pathway Modulation in Plant Protoplasts
Francesca De Marchis, Andrea Pompa, and Michele Bellucci
5. From Cytosol to the Apoplast: The Hygromycin Phosphotransferase (HYGR) Model in Arabidopsis
Haiyan Zhang and Jinjin Li
6. Following the Time-Course of Post-Pollination Events by Transmission Electron Microscopy (TEM): Buildup of Exosome-Like Structures with Compatible Pollinations
Darya Safavian, Jennifer Doucet, and Daphne R. Goring
Part III: UPS Contemplates Multidisciplinary Approaches for Animals and Fungi
7. Investigating Alternative Transport of Integral Plasma Membrane Proteins from the ER to the Golgi: Lessons from the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR)
Margarida D. Amaral, Carlos M. Farinha, Paulo Matos, and Hugo M. Botelho
8. Quantification of a Non-Conventional Protein Secretion: The Low Molecular Weight FGF-2 Example
Tania Arcondйguy, Christian Touriol, and Eric Lacazette
9. Human Primary Keratinocytes as a Tool for the Analysis of Caspase-1-Dependent Unconventional Protein Secretion
Gerhard E. Strittmatter, Martha Garstkiewicz, Jennifer Sand, Serena Grossi, Hans-Dietmar Beer
10. A Reporter System to Study Unconventional Secretion of Proteins Avoiding N-glycosylation in Ustilago maydis
Janpeter Stock, Marius Terfrьchte, and Kerstin Schipper
11. Stress-inducible Protein 1 (STI1): Extracellular Vesicles Analysis And Quantification
Marcos Vinicios Salles Dias, Vilma Regina Martins, and Glaucia Noeli Maroso Hajj
12. Analysis of Yeast Extracellular Vesicles
Marcio L. Rodrigues, Debora L. Oliveira, Gabriele Vargas, Wendell Girard-Dias, Anderson J. Franzen, Susana Frasйs, Kildare Miranda, and Leonardo Nimrichter
13. Exploring the Leishmania Hydrophilic Acylated Surface Protein B (HASPB) Export Pathway by Live Cell Imaging Methods
Lorna MacLean, Helen Price, and Peter O'Toole
14. Characterization of the Unconventional Secretion of the Ebola Matrix Protein VP40
Olivier Reynard and Mathieu Mateo
15. Role and Characterization of synuclein-γ Unconventional Protein Secretion in Cancer Cells
Caiyun Liu, Like Qu, and Chengchao Shou
Part IV: Secretome Isolation from Plant and Animal Samples to Identify Leaderless Secretory Proteins (LSP)
16. Characterization of the Tumor Secretome from Tumor Interstitial Fluid (TIF)
Pavel Gromov and Irina Gromova
17. Vacuum Infiltration-Centrifugation Method for Apoplastic Protein Extraction in Grapevine
Bertrand Delaunois, Fabienne Baillieul, Christophe Clйment, Philippe Jeandet, and Sylvain Cordelier
18. Isolation of Exosome-Like Vesicles from Plants by Ultracentrifugation on Sucrose/Deuterium Oxide (D2O) Density Cushions
Christopher Stanly, Immacolata Fiume, Giovambattista Capasso, and Gabriella Pocsfalvi
Автор: Schilling Oliver Название: Protein Terminal Profiling: Methods and Protocols ISBN: 1493983172 ISBN-13(EAN): 9781493983179 Издательство: Springer Рейтинг: Цена: 13275.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
Preface... Table of Contents... Contributing Authors...
1. [14C]-Acetyl-CoenzymeA-Based In Vitro N-Terminal Acetylation Assay Adrian Drazic and Thomas Arnesen 2. DTNB-Based Quantification of In Vitro Enzymatic N-Terminal Acetyltransferase Activity Hеvard Foyn, Paul R. Thompson, and Thomas Arnesen 3. SILProNAQ: A Convenient Approach for Proteome-Wide Analysis of Protein N-Termini and N-Terminal Acetylation Quantitation Willy V. Bienvenut, Carmela Giglione, and Thierry Meinnel 4. Profiling of Protein N-Termini and their Modifications in Complex Samples Fatih Demir, Stefan Niedermaier, Jayachandran N. Kizhakkedathu, and Pitter F. Huesgen 5. Protease Substrate Profiling by N-Terminal COFRADIC An Staes, Petra Van Damme, Evy Timmerman, Bart Ruttens, Elisabeth Stes, Kris Gevaert, and Francis Impens 6. Doublet N-Terminal Oriented Proteomics for N-Terminomics and Proteolytic Processing Identification Benoit Westermann, Alvaro Sebastian Vaca Jacome, Magali Rompais, Christine Carapito, and Christine Schaeffer-Reiss 7. Multidimensional Analysis of Protease Substrates and their Cellular Origins in Mixed Secretomes from Multiple Cell Types Pascal Schlage and Ulrich auf dem Keller 8. System-Wide Profiling of Protein Amino Termini from Formalin-Fixed, Paraffin-Embedded Tissue Specimens for the Identification of Novel Substrates Zon W. Lai and Oliver Schilling 9. Identification of Carboxypeptidase Substrates by C-Terminal COFRADIC Sebastian Tanco, Francesc Xavier Aviles, Kris Gevaert, Julia Lorenzo, and Petra Van Damme 10. ProC-TEL: Profiling of Protein C-Termini by Enzymatic Labeling Wenwen Duan and Guoqiang Xu 11. Determining Protease Substrates within a Complex Protein Background using the PROtein TOpography and Migration Analysis Platform (PROTOMAP) R.A. Fuhrman-Luck, L.M. Silva, M.L. Hastie, J.J. Gorman, J.A. Clements 12. Multiplexed Protease Specificity Profiling Using Isobaric Labeling Joanna Tucher and Andreas Tholey 13. FPPS - Fast Profiling of Protease Specificity Matej Vizovisek, Robert Vidmar, and Marko Fonovic 14. Profiling of Protease Cleavage Sites by Proteome-Derived Peptide Libraries and Quantitative Proteomics Chia-yi Chen, Bettina Mayer, and Oliver Schilling 15. Prediction of Proteases Involved in Peptide Generation Mercedes Arguello Casteleiro, Robert Stevens, and Julie Klein 16. Live-Cell Imaging of Protease Activity: Assays to Screen Therapeutic Approaches Anita Chalasani, Kyungmin Ji, Mansoureh Sameni, Samia H. Mazumder, Yong Xu, Kamiar Moin, and Bonnie F. Sloane 17. Protein Translocation Assays to Probe Protease Function and Screen for Inhibitors Angelina Hahlbrock, Dorothйe Gц wein, and Roland H. Stauber 18. Simultaneous Detection of Metalloprotease Activities in Complex Biological Samples Using the PrAMA (Proteolytic Activity Matrix Assay) Method Catharina Conrad, Miles A. Miller, Jцrg W Bartsch, Uwe Schlomann, Douglas A. Lauffenburger 19. Synthesis and Application of Activity-Based Probes for Proteases Tim Van Kersavond, Minh T. N. Nguyen, and Steven H. L. Verhelst
Автор: Pulido Rafael Название: Protein Tyrosine Phosphatases: Methods and Protocols ISBN: 1493981277 ISBN-13(EAN): 9781493981274 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This book provides coverage, methodology, and laboratory protocols on the more essential aspects of protein tyrosine phosphatase (PTP) function and regulation, including the use of standardized in vitro functional assays, suitable cell systems, and animal and microorganism models. Chapters covering state-of-the-art technical approaches suitable to decipher the physiologic roles of PTPs, and their involvement in tissue-specific functions, are also included, which will be of utility for both newcomers and experienced researchers in the field of tyrosine- and phosphoinositide- phosphorylation/dephosphorylation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Protein Tyrosine Phosphatases: Methods and Protocols aims to aid researchers in better defining the common and individual features of the PTP family members and translating this knowledge into PTP-based therapy for human disease.
Автор: Abbott D. Wade, Lammerts Van Bueren Alicia Название: Protein-Carbohydrate Interactions: Methods and Protocols ISBN: 1493983326 ISBN-13(EAN): 9781493983322 Издательство: Springer Рейтинг: Цена: 19564.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume is a wide-ranging tool for studying protein-carbohydrate interactions that extend from traditional biochemical methods to state-of-the-art techniques.
Автор: Vila-Perellу Miquel Название: Expressed Protein Ligation: Methods and Protocols ISBN: 1071604333 ISBN-13(EAN): 9781071604335 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Поставка под заказ.
Описание: This book provides a comprehensive overview of Expressed Protein Ligation (EPL), detailing methods and protocols to generate site-specifically modified proteins. Chapters include an overview of the protein semi-synthesis field, as well as related areas that have contributed to the development of EPL such as protein splicing and peptide synthesis. Following the introductory chapters, the rest of the book guides readers through protocols to perform EPL reactions, methods to synthesize peptide thioesters and to perform peptide and protein ligations, label proteins inside living cells, protocols for the semi-synthesis of phorphorylated, glycosylated and ubiquitylated proteins, synthesis and assembly of assymetrically modified nucleosomes, use of ligation auxiliaries and synthesis of cyclic proteins, as well as novel desulfurization strategies and use of selective Cys side chain protection to obtain precisely modified proteins.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Expressed Protein Ligation: Methods and Protocols will ensure successful implementation of protein semi-synthesis methods to further study the structure and function of proteins.
Описание: In the last few years, significant advances have been made in understanding how a yeast cell responds to the stress of producing a recombinant protein, and how this information can be used to engineer improved host strains. The molecular biology of the expression vector, through the choice of promoter, tag and codon optimization of the target gene, is also a key determinant of a high-yielding protein production experiment. Recombinant Protein Production in Yeast: Methods and Protocols examines the process of preparation of expression vectors, transformation to generate high-yielding clones, optimization of experimental conditions to maximize yields, scale-up to bioreactor formats and disruption of yeast cells to enable the isolation of the recombinant protein prior to purification. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Recombinant Protein Production in Yeast: Methods and Protocols, seeks to aid scientists in adopting yeast as a protein production host.
Описание: In this present volume, different approaches are detailed to produce membrane proteins, purify them, study their function, determine their structure, and model them in membrane.
Автор: Kleinschmidt Jцrg H. Название: Lipid-Protein Interactions: Methods and Protocols ISBN: 1493962639 ISBN-13(EAN): 9781493962631 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Biological membranes are the essential structuring elements of all living cells. Many enzymatic reactions take place at the membrane-water interface. To gain detailed insight into membrane properties, it is therefore of great importance to understand the complex nature of the interactions of membrane proteins with lipids. Lipid-Protein Interactions: Methods and Protocols provides a selection of protocols to examine protein-lipid interactions, membrane and membrane protein structure, how membrane proteins affect lipids and how they are in turn affected by the lipid bilayer and lipid properties. The methods described here are all actively used, complementary, and necessary to obtain comprehensive information about membrane structure and function. They include label-free approaches, imaging techniques and spectroscopic methodologies. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Lipid-Protein Interactions: Methods and Protocols seeks to serve both professional and novices with its wide range of the methods frequently used in this area of research.
Описание: This volume discusses protocols that cover genetic manipulation of Chinese hamster ovary (CHO) cells for recombinant protein production, and protocols for the characterization of CHO cells using ‘omic approaches. This book also explores methods that discuss the genome editing tool, CRISPR/Cas9, and the characterization of recombinant protein products, such as glycosylation and host cell protein analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Heterologous Protein Production in CHO Cells: Methods and Protocols is a valuable resource for scientists and researchers who are interested in further studying cell production in CHO cells.
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