Heterologous Protein Production in Cho Cells: Methods and Protocols, Meleady Paula

Автор: Paula Meleady
Название: Heterologous Protein Production in CHO Cells
ISBN: 1493969714 ISBN-13(EAN): 9781493969715
Издательство: Springer
Цена: 20962.00 р.
Наличие на складе: Есть у поставщикаПоставка под заказ.
Описание: This volume discusses protocols that cover genetic manipulation of Chinese hamster ovary (CHO) cells for recombinant protein production, and protocols for the characterization of CHO cells using `omic approaches.

Автор: Paula Meleady
Название: Heterologous Protein Production in CHO Cells
ISBN: 1493969714 ISBN-13(EAN): 9781493969715
Издательство: Springer
Рейтинг:
Цена: 20962.00 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание: This volume discusses protocols that cover genetic manipulation of Chinese hamster ovary (CHO) cells for recombinant protein production, and protocols for the characterization of CHO cells using `omic approaches.

Автор: Kihara Daisuke
Название: Protein Function Prediction: Methods and Protocols
ISBN: 1493983687 ISBN-13(EAN): 9781493983681
Издательство: Springer
Рейтинг:
Цена: 19564.00 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание: Preface...
Table of Contents...
Contributing Authors...

1. Using PFP and ESG Protein Function Prediction Web Servers
Qing Wei, Joshua McGraw, Ishita Khan, and Daisuke Kihara

2. GHOSTX: A Fast Sequence Homology Search Tool for Functional Annotation of Metagenomic Data
Shuji Suzuki, Takashi Ishida, Masahito Ohue, Masanori Kakuta, and Yutaka Akiyama

3. From Gene Annotation to Function Prediction for Metagenomics
Fatemeh Sharifi and Yuzhen Ye

4. An Agile Functional Analysis of Metagenomic Data using SUPER-FOCUS
Genivaldo Gueiros Z. Silva, Fabyano A. C. Lopes, and Robert A. Edwards

5. MPFit: Computational Tool for Predicting Moonlighting Proteins
Ishita Khan, Joshua McGraw, and Daisuke Kihara

6. Predicting Secretory Proteins with SignalP
Henrik Nielsen

7. The ProFunc Function Prediction Server
Roman A. Laskowski

8. G-LoSA for Prediction of Protein-Ligand Binding Sites and Structures
Hui Sun Lee and Wonpil Im

9. Local Alignment of Ligand Binding Sites in Proteins for Polypharmacology and Drug Repositioning
Michal Brylinski

10. WATsite2.0 with PyMOL Plugin: Hydration Site Prediction and Visualization
Ying Yang, Bingjie Hu, and Markus A. Lill

11. Enzyme Annotation and Metabolic Reconstruction Using KEGG
Minoru Kanehisa

12. Ortholog Identification and Comparative Analysis of Microbial Genomes using MBGD and RECOG
Ikuo Uchiyama

13. Exploring Protein Function Using the Saccharomyces Genome Database
Edith D. Wong

14. Network-Based Gene Function Prediction in Mouse and Other Model Vertebrates using MouseNet Server
Eiru Kim and Insuk Lee

15. The FANTOM5 Computation Ecosystem: Genomic Information Hub for Promoters and Active Enhancers
Imad Abugessaisa, Shuhei Noguchi, Piero Carninci, and Takeya Kasukawa

16. Multi-Algorithm Particle Simulations with Spatiocyte
Satya N. V. Arjunan and Koichi Takahashi

Автор: Isabelle Mus-Veteau
Название: Heterologous Expression of Membrane Proteins
ISBN: 1493961322 ISBN-13(EAN): 9781493961320
Издательство: Springer
Рейтинг:
Цена: 16769.00 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание: This book provides an overview of the different heterologous expression systems available to produce various membrane proteins for structural analysis. Chapters examine membrane protein solubilization, purification, and instability in solution.

Автор: Mus-Veteau
Название: Heterologous Expression of Membrane Proteins
ISBN: 1493936352 ISBN-13(EAN): 9781493936359
Издательство: Springer
Рейтинг:
Цена: 19564.00 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание: This detailed volume encompasses chapters from leadingexperts in the area of membrane proteins who describe step-by-step protocols developed these last few years to improve the functionalproduction and stabilization of recombinant integral membrane proteins (IMPs). Membraneproteins play a key role in numerous pathologies such as cancer, cysticfibrosis, epilepsy, hyperinsulinism, and Alzheimer’s disease, yet studies onthese and other disorders are hampered by a lack of information about theproteins involved. This book sets out to aid researchers in rectifying thissituation. Written for the highly successful Methods in Molecular Biology series, chapters include introductionsto their respective topics, lists of the necessary materials and reagents,readily reproducible laboratory protocols, and tips on troubleshooting andavoiding known pitfalls.Authoritative and up-to-date, HeterologousExpression of Membrane Proteins: Methods and Protocols, Second Editionserves as an ideal guide for scientists attempting to delve deeper into the myriadunique IMP structures.

Автор: Thomas C. Evans, Jr.; Ming-Qun Xu
Название: Heterologous Gene Expression in E.coli
ISBN: 1493957252 ISBN-13(EAN): 9781493957255
Издательство: Springer
Рейтинг:
Цена: 20263.00 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание: Practical and cutting-edge, Heterologous Gene Expression in E. coli: Methods and Protocols seeks to familiarize the researcher with the myriad of E. coli expression strains available and move E. coli closer to that ideal of the perfect host.

Автор: Vila-Perellу Miquel
Название: Expressed Protein Ligation: Methods and Protocols
ISBN: 1071604333 ISBN-13(EAN): 9781071604335
Издательство: Springer
Рейтинг:
Цена: 15372.00 р.
Наличие на складе: Поставка под заказ.

Описание: This book provides a comprehensive overview of Expressed Protein Ligation (EPL), detailing methods and protocols to generate site-specifically modified proteins. Chapters include an overview of the protein semi-synthesis field, as well as related areas that have contributed to the development of EPL such as protein splicing and peptide synthesis. Following the introductory chapters, the rest of the book guides readers through protocols to perform EPL reactions, methods to synthesize peptide thioesters and to perform peptide and protein ligations, label proteins inside living cells, protocols for the semi-synthesis of phorphorylated, glycosylated and ubiquitylated proteins, synthesis and assembly of assymetrically modified nucleosomes, use of ligation auxiliaries and synthesis of cyclic proteins, as well as novel desulfurization strategies and use of selective Cys side chain protection to obtain precisely modified proteins.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Expressed Protein Ligation: Methods and Protocols will ensure successful implementation of protein semi-synthesis methods to further study the structure and function of proteins.

Автор: Schueler-Furman, Ora;London, Nir
Название: Modeling Peptide-Protein Interactions : Methods and Protocols
ISBN: 1493967967 ISBN-13(EAN): 9781493967964
Издательство: Springer
Рейтинг:
Цена: 13974.00 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание: This volume covers an array of techniques available for studying peptide-protein docking and design.

Автор: Schueler-Furman Ora, London Nir
Название: Modeling Peptide-Protein Interactions: Methods and Protocols
ISBN: 1493983024 ISBN-13(EAN): 9781493983025
Издательство: Springer
Рейтинг:
Цена: 11878.00 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание: This volume covers an array of techniques available for studying peptide-protein docking and design. The book is divided into four sections: peptide binding site prediction; peptide-protein docking; prediction and design of peptide binding specificity; and the design of inhibitory peptides. The chapters in Modeling Peptide-Protein Interactions: Methods and Protocols cover topics such as the usage of ACCLUSTER and PeptiMap for peptide binding site prediction; AnchorDock and ATTRACT for blind, flexible docking of peptides to proteins; flexible peptide docking using HADDOCK and FlexPepDock; identifying loop-mediated protein-protein interactions using LoopFinder; and protein-peptide interaction design using PinaColada. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary details for successful application of the different approaches and step-by-step, readily reproducible protocols, as well as tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Modeling Peptide-Protein Interactions: Methods and Protocols provides a diverse and unified overview of this rapidly advancing field of major interest and applicability.

Автор: Schilling Oliver
Название: Protein Terminal Profiling: Methods and Protocols
ISBN: 1493983172 ISBN-13(EAN): 9781493983179
Издательство: Springer
Рейтинг:
Цена: 13275.00 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание:

Preface...
Table of Contents...
Contributing Authors...

1. [¹⁴C]-Acetyl-CoenzymeA-Based In Vitro N-Terminal Acetylation Assay
Adrian Drazic and Thomas Arnesen

2. DTNB-Based Quantification of In Vitro Enzymatic N-Terminal Acetyltransferase Activity
Hеvard Foyn, Paul R. Thompson, and Thomas Arnesen

3. SILProNAQ: A Convenient Approach for Proteome-Wide Analysis of Protein N-Termini and N-Terminal Acetylation Quantitation
Willy V. Bienvenut, Carmela Giglione, and Thierry Meinnel

4. Profiling of Protein N-Termini and their Modifications in Complex Samples
Fatih Demir, Stefan Niedermaier, Jayachandran N. Kizhakkedathu, and Pitter F. Huesgen

5. Protease Substrate Profiling by N-Terminal COFRADIC
An Staes, Petra Van Damme, Evy Timmerman, Bart Ruttens, Elisabeth Stes, Kris Gevaert, and Francis Impens

6. Doublet N-Terminal Oriented Proteomics for N-Terminomics and Proteolytic Processing Identification
Benoit Westermann, Alvaro Sebastian Vaca Jacome, Magali Rompais, Christine Carapito, and Christine Schaeffer-Reiss

7. Multidimensional Analysis of Protease Substrates and their Cellular Origins in Mixed Secretomes from Multiple Cell Types
Pascal Schlage and Ulrich auf dem Keller

8. System-Wide Profiling of Protein Amino Termini from Formalin-Fixed, Paraffin-Embedded Tissue Specimens for the Identification of Novel Substrates
Zon W. Lai and Oliver Schilling

9. Identification of Carboxypeptidase Substrates by C-Terminal COFRADIC
Sebastian Tanco, Francesc Xavier Aviles, Kris Gevaert, Julia Lorenzo, and Petra Van Damme

10. ProC-TEL: Profiling of Protein C-Termini by Enzymatic Labeling
Wenwen Duan and Guoqiang Xu

11. Determining Protease Substrates within a Complex Protein Background using the PROtein TOpography and Migration Analysis Platform (PROTOMAP)
R.A. Fuhrman-Luck, L.M. Silva, M.L. Hastie, J.J. Gorman, J.A. Clements

12. Multiplexed Protease Specificity Profiling Using Isobaric Labeling
Joanna Tucher and Andreas Tholey

13. FPPS - Fast Profiling of Protease Specificity
Matej Vizovisek, Robert Vidmar, and Marko Fonovic

14. Profiling of Protease Cleavage Sites by Proteome-Derived Peptide Libraries and Quantitative Proteomics
Chia-yi Chen, Bettina Mayer, and Oliver Schilling

15. Prediction of Proteases Involved in Peptide Generation
Mercedes Arguello Casteleiro, Robert Stevens, and Julie Klein

16. Live-Cell Imaging of Protease Activity: Assays to Screen Therapeutic Approaches
Anita Chalasani, Kyungmin Ji, Mansoureh Sameni, Samia H. Mazumder, Yong Xu, Kamiar Moin, and Bonnie F. Sloane

17. Protein Translocation Assays to Probe Protease Function and Screen for Inhibitors
Angelina Hahlbrock, Dorothйe Gц wein, and Roland H. Stauber

18. Simultaneous Detection of Metalloprotease Activities in Complex Biological Samples Using the PrAMA (Proteolytic Activity Matrix Assay) Method
Catharina Conrad, Miles A. Miller, Jцrg W Bartsch, Uwe Schlomann, Douglas A. Lauffenburger

19. Synthesis and Application of Activity-Based Probes for Proteases
Tim Van Kersavond, Minh T. N. Nguyen, and Steven H. L. Verhelst

Автор: Walls Dermot, Loughran Sinйad T.
Название: Protein Chromatography: Methods and Protocols
ISBN: 1493961292 ISBN-13(EAN): 9781493961290
Издательство: Springer
Рейтинг:
Цена: 15372.00 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание: A prerequisite for elucidating the structure and function of any protein is the prior purification of that protein. This necessity has led to the development of many purification schemes and chromatographic methods for the isolation of native proteins from complex sources. In Protein Chromatography: Methods and Protocols, leading researchers present clear protocol-style chapters that are suitable for newcomers and experts alike. The book opens with vital topics in protein biochemistry, addressing such areas as protein stability and storage, avoiding proteolysis during chromatography, protein quantitation methods including immuno-qPCR, and the contrasting challenges that microfluidics and scale-up production pose to the investigator, and then it segues into key methods involving the generation and purification of recombinant proteins through recombinant antibody production and the tagging of proteins, amongst other means, as well as many variations on classic techniques such as ion-exchange and immunoaffinity chromatography. Written in the highly successful Methods in Molecular Biology™ series format, protocols chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Protein Chromatography: Methods and Protocols will greatly aid scientists in establishing these essential techniques in their own laboratories and furthering our understanding of the many imperative functions of proteins.

Автор: Abbott D. Wade, Lammerts Van Bueren Alicia
Название: Protein-Carbohydrate Interactions: Methods and Protocols
ISBN: 1493983326 ISBN-13(EAN): 9781493983322
Издательство: Springer
Рейтинг:
Цена: 19564.00 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание: This volume is a wide-ranging tool for studying protein-carbohydrate interactions that extend from traditional biochemical methods to state-of-the-art techniques.

Автор: Kister Alexander E.
Название: Protein Supersecondary Structures: Methods and Protocols
ISBN: 1493991604 ISBN-13(EAN): 9781493991600
Издательство: Springer
Рейтинг:
Цена: 20962.00 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание: