Protein Terminal Profiling: Methods and Protocols, Schilling Oliver
Автор: Anton Posch Название: Proteomic Profiling ISBN: 1493925490 ISBN-13(EAN): 9781493925490 Издательство: Springer Рейтинг: Цена: 30745.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume presents the latest developments of the main pillars of protein analysis, such as sample preparation, separation and characterization. The book begins by describing basic but important sample preparation protocols. It then goes on to describe more sophisticated procedures on enriching specific protein classes and concludes with detailed descriptions of integrated work-flows for comprehensive protein analysis and characterization. The authors of the individual chapters are renowned protein biochemists who have all set value to provide a detailed representation of their lab work. Throughout the chapters, these authors share important tips and tricks for a successful and reproducible employment of their protocols in other laboratories. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Proteomic Profiling: Methods and Protocols is the perfect guide for students of Biochemistry, Biomedicine, Biology, and Genomics and will be an invaluable source for the experienced, practicing scientists.
Автор: Kister Alexander E. Название: Protein Supersecondary Structures: Methods and Protocols ISBN: 1493991604 ISBN-13(EAN): 9781493991600 Издательство: Springer Рейтинг: Цена: 20962.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
This second edition volume expands on the previous edition with an update on the latest developments in the field and new techniques used to study secondary and supersecondary structures (SSS) in proteins. Chapters in this book discuss topics such as sequence and structural features of different SSS elements; software used for the automatic annotation of secondary structure elements in proteins; the new developments in secondary and SSS prediction and the modern approaches for energy landscape calculation; protein misfolding and amyloid structural formation; analysis of ‘transformer proteins’; discovery of the structure of the hydrophobic nucleus; and discussions of the main principles of protein structures formation. The contributing authors of the volume are the eminent experts in the field of protein research and bioinformatics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and authoritative, Protein Supersecondary Structures: Methods and Protocols, Second Edition is a valuable resource for researchers who are interested in learning more about the relationship between amino acids sequences and protein structures, the evolution of proteins and the dynamics of protein formation.
Автор: Schueler-Furman Ora, London Nir Название: Modeling Peptide-Protein Interactions: Methods and Protocols ISBN: 1493983024 ISBN-13(EAN): 9781493983025 Издательство: Springer Рейтинг: Цена: 11878.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume covers an array of techniques available for studying peptide-protein docking and design. The book is divided into four sections: peptide binding site prediction; peptide-protein docking; prediction and design of peptide binding specificity; and the design of inhibitory peptides. The chapters in Modeling Peptide-Protein Interactions: Methods and Protocols cover topics such as the usage of ACCLUSTER and PeptiMap for peptide binding site prediction; AnchorDock and ATTRACT for blind, flexible docking of peptides to proteins; flexible peptide docking using HADDOCK and FlexPepDock; identifying loop-mediated protein-protein interactions using LoopFinder; and protein-peptide interaction design using PinaColada. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary details for successful application of the different approaches and step-by-step, readily reproducible protocols, as well as tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Modeling Peptide-Protein Interactions: Methods and Protocols provides a diverse and unified overview of this rapidly advancing field of major interest and applicability.
Автор: Oliver Schilling Название: Protein Terminal Profiling ISBN: 1493968491 ISBN-13(EAN): 9781493968497 Издательство: Springer Рейтинг: Цена: 13974.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
Preface... Table of Contents... Contributing Authors...
1. [14C]-Acetyl-CoenzymeA-Based In Vitro N-Terminal Acetylation Assay Adrian Drazic and Thomas Arnesen 2. DTNB-Based Quantification of In Vitro Enzymatic N-Terminal Acetyltransferase Activity Hеvard Foyn, Paul R. Thompson, and Thomas Arnesen 3. SILProNAQ: A Convenient Approach for Proteome-Wide Analysis of Protein N-Termini and N-Terminal Acetylation Quantitation Willy V. Bienvenut, Carmela Giglione, and Thierry Meinnel 4. Profiling of Protein N-Termini and their Modifications in Complex Samples Fatih Demir, Stefan Niedermaier, Jayachandran N. Kizhakkedathu, and Pitter F. Huesgen 5. Protease Substrate Profiling by N-Terminal COFRADIC An Staes, Petra Van Damme, Evy Timmerman, Bart Ruttens, Elisabeth Stes, Kris Gevaert, and Francis Impens 6. Doublet N-Terminal Oriented Proteomics for N-Terminomics and Proteolytic Processing Identification Benoit Westermann, Alvaro Sebastian Vaca Jacome, Magali Rompais, Christine Carapito, and Christine Schaeffer-Reiss 7. Multidimensional Analysis of Protease Substrates and their Cellular Origins in Mixed Secretomes from Multiple Cell Types Pascal Schlage and Ulrich auf dem Keller 8. System-Wide Profiling of Protein Amino Termini from Formalin-Fixed, Paraffin-Embedded Tissue Specimens for the Identification of Novel Substrates Zon W. Lai and Oliver Schilling 9. Identification of Carboxypeptidase Substrates by C-Terminal COFRADIC Sebastian Tanco, Francesc Xavier Aviles, Kris Gevaert, Julia Lorenzo, and Petra Van Damme 10. ProC-TEL: Profiling of Protein C-Termini by Enzymatic Labeling Wenwen Duan and Guoqiang Xu 11. Determining Protease Substrates within a Complex Protein Background using the PROtein TOpography and Migration Analysis Platform (PROTOMAP) R.A. Fuhrman-Luck, L.M. Silva, M.L. Hastie, J.J. Gorman, J.A. Clements 12. Multiplexed Protease Specificity Profiling Using Isobaric Labeling Joanna Tucher and Andreas Tholey 13. FPPS - Fast Profiling of Protease Specificity Matej Vizovisek, Robert Vidmar, and Marko Fonovic 14. Profiling of Protease Cleavage Sites by Proteome-Derived Peptide Libraries and Quantitative Proteomics Chia-yi Chen, Bettina Mayer, and Oliver Schilling 15. Prediction of Proteases Involved in Peptide Generation Mercedes Arguello Casteleiro, Robert Stevens, and Julie Klein 16. Live-Cell Imaging of Protease Activity: Assays to Screen Therapeutic Approaches Anita Chalasani, Kyungmin Ji, Mansoureh Sameni, Samia H. Mazumder, Yong Xu, Kamiar Moin, and Bonnie F. Sloane 17. Protein Translocation Assays to Probe Protease Function and Screen for Inhibitors Angelina Hahlbrock, Dorothйe Gц wein, and Roland H. Stauber 18. Simultaneous Detection of Metalloprotease Activities in Complex Biological Samples Using the PrAMA (Proteolytic Activity Matrix Assay) Method Catharina Conrad, Miles A. Miller, Jцrg W Bartsch, Uwe Schlomann, Douglas A. Lauffenburger 19. Synthesis and Application of Activity-Based Probes for Proteases Tim Van Kersavond, Minh T. N. Nguyen, and Steven H. L. Verhelst
Автор: Mathias Munschauer Название: High-Resolution Profiling of Protein-RNA Interactions ISBN: 3319162527 ISBN-13(EAN): 9783319162522 Издательство: Springer Рейтинг: Цена: 18167.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: The work reported in this book represents an excellent example of how creative experimentation and technology development, complemented by computational data analysis, can yield important insights that further our understanding of biological entities from a systems perspective. The book describes how the study of a single RNA-binding protein and its interaction sites led to the development of the novel ‘protein occupancy profiling’ technology that for the first time captured the mRNA sequence space contacted by the ensemble of expressed RNA binders. Application of protein occupancy profiling to eukaryotic cells revealed that extensive sequence stretches in 3’ UTRs can be contacted by RBPs and that evolutionary conservation as well as negative selection act on protein-RNA contact sites, suggesting functional importance. Comparative analysis of the RBP-bound sequence space has the potential to unravel putative cis-acting RNA elements without a priori knowledge of the bound regulators. Here, Dr. Munschauer provides a comprehensive introduction to the field of post-transcriptional gene regulation, examines state-of-the-art technologies, and combines the conclusions from several journal articles into a coherent and logical story from the frontiers of systems-biology inspired life science. This thesis, submitted to the Department of Biology, Chemistry and Pharmacy at Freie Universit?t Berlin, was selected as outstanding work by the Berlin Institute for Medical Systems Biology at the Max-Delbrueck Center for Molecular Medicine, Germany.
Автор: Stephan A. Sieber Название: Activity-Based Protein Profiling ISBN: 3642448313 ISBN-13(EAN): 9783642448317 Издательство: Springer Рейтинг: Цена: 36570.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: SieberActivity-Based Protein Profiling for Natural Product Target Discovery, by Joanna Krysiak und Rolf BreinbauerPhotoaffinity Labeling in Activity-Based Protein Profiling, by Paul P. TateFunctional Analysis of Protein Targets by Metabolomic Approaches, by Yun-Gon Kim und Alan Saghatelian
Автор: Hicham Zegzouti; Said A. Goueli Название: Kinase Screening and Profiling ISBN: 1493949233 ISBN-13(EAN): 9781493949236 Издательство: Springer Рейтинг: Цена: 13275.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
1. HTRF Kinase Assay Development and Methods in Inhibitor Characterization Yong Jia, Mari Manuia, and Jose Juarez
2. Application of Eukaryotic Elongation Factor-2 Kinase (eEF-2K) for Cancer Therapy: Expression, Purification, and High-Throughput Inhibitor Screening Clint D. J. Tavares, Ashwini K. Devkota, Kevin N. Dalby, and Eun Jeong Cho 3. Recombinant Kinase Production and Fragment Screening by NMR Spectroscopy Byeonggu Han and Hee-Chul Ahn 4. Bioluminescence Methods for Assaying Kinases in Quantitative High-Throughput Screening (qHTS) Format Applied to Yes1 Tyrosine Kinase, Glucokinase and PI5P4Kα Lipid Kinase Mindy I. Davis, Douglas S. Auld, and James Inglese 5. Using Bioluminescent Kinase Profiling Strips to Identify Kinase Inhibitor Selectivity and Promiscuity Hicham Zegzouti, Jacquelyn Hennek, and Said A. Goueli 6. Measuring Activity of Phosphoinositide Lipid Kinases Using a Bioluminescent ADP-Detecting Assay Andrew W. Tai and Jolanta Vidugiriene
7. A High-Throughput Radiometric Kinase Assay Krisna C. Duong-Ly and Jeffrey R. Peterson 8. A High Content Assay to Screen for Modulators of EGFR Function Christophe Antczak and Hakim Djaballah 9. Monitoring Protein Kinase Expression and Phosphorylation in Cell Lysates with Antibody Microarrays Hong Zhang, Xiaoqing Shi, and Steven Pelech
10. From Enzyme to Whole Blood: Sequential Screening Procedure for Identification and Evaluation of p38 MAPK Inhibitors Silke M. Bauer, Jakub M. Kubiak, Ulrich Rothbauer, and Stefan Laufer
11. Genetically Encoded Fluorescent Indicators to Visualize Protein Phosphorylation in Living Cells Moritoshi Sato and Yoshio Umezawa
12. Characterization of an Engineered Src Kinase to Study Src Signaling and Biology Leanna R. Gentry, Andrei V. Karginov, Klaus M. Hahn, and Channing J. Der
13. Screening One-Bead-One-Compound Peptide Libraries for Optimal Kinase Substrates Thi B. Trinh and Dehua Pei
14. Determination of the Substrate Specificity of Protein Kinases with Peptide Micro- and Macroarrays Shenshen Lai, Dirk F.H. Winkler, Hong Zhang, and Steven Pelech 15. Rapid Identification of Protein Kinase Phosphorylation Site Motifs Using Combinatorial Peptide Libraries Chad J. Miller and Benjamin E. Turk
Автор: Karamanos Yannis Название: Expression Profiling in Neuroscience ISBN: 149396223X ISBN-13(EAN): 9781493962235 Издательство: Springer Рейтинг: Цена: 16769.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Transcriptomics and proteomics, studying the profile of the expression of nucleic acids and proteins respectively, are increasingly applied to gain a mechanistic insight into a wide spectrum of investigation, and the use of expression profiling studies for the central nervous system and brain function aids in the understanding of neurodegenerative disorders and tumor development mechanisms. In Expression Profiling in Neuroscience, expert researchers provide a survey of the most commonly used approaches in the field and scan the different ways of studying the central nervous system/brain environment through expression profiling. The first part addresses the gene expression profiling of the brain at a large scale or to a specific cell type such as blood-brain barrier endothelium. Then, the second part describes the protein expression studies and the different technologies applied. Written for the popular Neuromethods series, chapters include corresponding background information, tested laboratory protocols, and step-by-step methods for reproducible laboratory experiments. Detailed and authoritative, Expression Profiling in Neuroscience presents the state-of-the-art techniques necessary to expand research further into this vital area of study.
Автор: Pawel Ciborowski Название: Proteomic Profiling and Analytical Chemistry ISBN: 0444636889 ISBN-13(EAN): 9780444636881 Издательство: Elsevier Science Рейтинг: Цена: 17517.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
"Proteomic Profiling and Analytical Chemistry: The Crossroads, Second Edition" helps scientists without a strong background in analytical chemistry to understand principles of the multistep proteomic experiment necessary for its successful completion. It also helps researchers who do have an analytical chemistry background to break into the proteomics field. Highlighting points of junction between proteomics and analytical chemistry, this resource links experimental design with analytical measurements, data analysis, and quality control. This targeted point of view will help both biologists and chemists to better understand all components of a complex proteomic study.
The book provides detailed coverage of experimental aspects such as sample preparation, protein extraction and precipitation, gel electrophoresis, microarrays, dynamics of fluorescent dyes, and more. The key feature of this book is a direct link between multistep proteomic strategy and quality control routinely applied in analytical chemistry. This second edition features a new chapter on SWATH-MS, substantial updates to all chapters, including proteomic database search and analytical quantification, expanded discussion of post-hoc statistical tests, and additional content on validation in proteomics. Covers the analytical consequences of protein and peptide modifications that may have a profound effect on how and what researchers actually measureIncludes practical examples illustrating the importance of problems in quantitation and validation of biomarkersHelps in designing and executing proteomic experiments with sound analytics
Автор: Pompa Andrea, De Marchis Francesca Название: Unconventional Protein Secretion: Methods and Protocols ISBN: 1493981447 ISBN-13(EAN): 9781493981441 Издательство: Springer Рейтинг: Цена: 6986.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
Part I: Background
1. ER to Golgi Dependent Protein Secretion: The Conventional Pathway
Corrado Viotti
2. Unconventional Protein Secretion in Animal Cells
Fanny Ng and Bor Luen Tang
3. Unconventional Protein Secretion in Plants
Destiny J. Davis, Byung-Ho Kang, Angelo S. Heringer, Thomas E. Wilkop, and Georgia Drakakaki
Part II: UPS Contemplates Multidisciplinary Approaches for Plants
4. Chemical Secretory Pathway Modulation in Plant Protoplasts
Francesca De Marchis, Andrea Pompa, and Michele Bellucci
5. From Cytosol to the Apoplast: The Hygromycin Phosphotransferase (HYGR) Model in Arabidopsis
Haiyan Zhang and Jinjin Li
6. Following the Time-Course of Post-Pollination Events by Transmission Electron Microscopy (TEM): Buildup of Exosome-Like Structures with Compatible Pollinations
Darya Safavian, Jennifer Doucet, and Daphne R. Goring
Part III: UPS Contemplates Multidisciplinary Approaches for Animals and Fungi
7. Investigating Alternative Transport of Integral Plasma Membrane Proteins from the ER to the Golgi: Lessons from the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR)
Margarida D. Amaral, Carlos M. Farinha, Paulo Matos, and Hugo M. Botelho
8. Quantification of a Non-Conventional Protein Secretion: The Low Molecular Weight FGF-2 Example
Tania Arcondйguy, Christian Touriol, and Eric Lacazette
9. Human Primary Keratinocytes as a Tool for the Analysis of Caspase-1-Dependent Unconventional Protein Secretion
Gerhard E. Strittmatter, Martha Garstkiewicz, Jennifer Sand, Serena Grossi, Hans-Dietmar Beer
10. A Reporter System to Study Unconventional Secretion of Proteins Avoiding N-glycosylation in Ustilago maydis
Janpeter Stock, Marius Terfrьchte, and Kerstin Schipper
11. Stress-inducible Protein 1 (STI1): Extracellular Vesicles Analysis And Quantification
Marcos Vinicios Salles Dias, Vilma Regina Martins, and Glaucia Noeli Maroso Hajj
12. Analysis of Yeast Extracellular Vesicles
Marcio L. Rodrigues, Debora L. Oliveira, Gabriele Vargas, Wendell Girard-Dias, Anderson J. Franzen, Susana Frasйs, Kildare Miranda, and Leonardo Nimrichter
13. Exploring the Leishmania Hydrophilic Acylated Surface Protein B (HASPB) Export Pathway by Live Cell Imaging Methods
Lorna MacLean, Helen Price, and Peter O'Toole
14. Characterization of the Unconventional Secretion of the Ebola Matrix Protein VP40
Olivier Reynard and Mathieu Mateo
15. Role and Characterization of synuclein-γ Unconventional Protein Secretion in Cancer Cells
Caiyun Liu, Like Qu, and Chengchao Shou
Part IV: Secretome Isolation from Plant and Animal Samples to Identify Leaderless Secretory Proteins (LSP)
16. Characterization of the Tumor Secretome from Tumor Interstitial Fluid (TIF)
Pavel Gromov and Irina Gromova
17. Vacuum Infiltration-Centrifugation Method for Apoplastic Protein Extraction in Grapevine
Bertrand Delaunois, Fabienne Baillieul, Christophe Clйment, Philippe Jeandet, and Sylvain Cordelier
18. Isolation of Exosome-Like Vesicles from Plants by Ultracentrifugation on Sucrose/Deuterium Oxide (D2O) Density Cushions
Christopher Stanly, Immacolata Fiume, Giovambattista Capasso, and Gabriella Pocsfalvi
Автор: Pulido Rafael Название: Protein Tyrosine Phosphatases: Methods and Protocols ISBN: 1493981277 ISBN-13(EAN): 9781493981274 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This book provides coverage, methodology, and laboratory protocols on the more essential aspects of protein tyrosine phosphatase (PTP) function and regulation, including the use of standardized in vitro functional assays, suitable cell systems, and animal and microorganism models. Chapters covering state-of-the-art technical approaches suitable to decipher the physiologic roles of PTPs, and their involvement in tissue-specific functions, are also included, which will be of utility for both newcomers and experienced researchers in the field of tyrosine- and phosphoinositide- phosphorylation/dephosphorylation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Protein Tyrosine Phosphatases: Methods and Protocols aims to aid researchers in better defining the common and individual features of the PTP family members and translating this knowledge into PTP-based therapy for human disease.
Автор: Abbott D. Wade, Lammerts Van Bueren Alicia Название: Protein-Carbohydrate Interactions: Methods and Protocols ISBN: 1493983326 ISBN-13(EAN): 9781493983322 Издательство: Springer Рейтинг: Цена: 19564.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume is a wide-ranging tool for studying protein-carbohydrate interactions that extend from traditional biochemical methods to state-of-the-art techniques.
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