Unconventional Protein Secretion: Methods and Protocols, Pompa Andrea, De Marchis Francesca
Автор: Pompa Название: Unconventional Protein Secretion ISBN: 1493938029 ISBN-13(EAN): 9781493938025 Издательство: Springer Рейтинг: Цена: 13275.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume presents relevantbackground information to understanding the molecular basis governingunconventional protein secretion (UPS), and in particular explores the latesttechniques and protocols that have been successfully applied for the study ofthis topic. Detailed chapters include an overview of conventional andunconventional secretory pathways along with multidisciplinary approaches andmethods used for UPS analysis in different organisms. Written in the highly successful Methods inMolecular Biology series format, chapters include introductions to theirrespective topics, lists of the necessary materials and reagents, step-by-step,readily reproducible laboratory protocols, and tips on troubleshooting andavoiding known pitfalls. Authoritative and cutting-edge, UnconventionalProtein Secretion: Methods and Protocols will be usefulfor all interested in the secretory pathway field as well as applications incell biology, cell development, biomedical research, and healthcare.
Автор: Schilling Oliver Название: Protein Terminal Profiling: Methods and Protocols ISBN: 1493983172 ISBN-13(EAN): 9781493983179 Издательство: Springer Рейтинг: Цена: 13275.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
Preface... Table of Contents... Contributing Authors...
1. [14C]-Acetyl-CoenzymeA-Based In Vitro N-Terminal Acetylation Assay Adrian Drazic and Thomas Arnesen 2. DTNB-Based Quantification of In Vitro Enzymatic N-Terminal Acetyltransferase Activity Hеvard Foyn, Paul R. Thompson, and Thomas Arnesen 3. SILProNAQ: A Convenient Approach for Proteome-Wide Analysis of Protein N-Termini and N-Terminal Acetylation Quantitation Willy V. Bienvenut, Carmela Giglione, and Thierry Meinnel 4. Profiling of Protein N-Termini and their Modifications in Complex Samples Fatih Demir, Stefan Niedermaier, Jayachandran N. Kizhakkedathu, and Pitter F. Huesgen 5. Protease Substrate Profiling by N-Terminal COFRADIC An Staes, Petra Van Damme, Evy Timmerman, Bart Ruttens, Elisabeth Stes, Kris Gevaert, and Francis Impens 6. Doublet N-Terminal Oriented Proteomics for N-Terminomics and Proteolytic Processing Identification Benoit Westermann, Alvaro Sebastian Vaca Jacome, Magali Rompais, Christine Carapito, and Christine Schaeffer-Reiss 7. Multidimensional Analysis of Protease Substrates and their Cellular Origins in Mixed Secretomes from Multiple Cell Types Pascal Schlage and Ulrich auf dem Keller 8. System-Wide Profiling of Protein Amino Termini from Formalin-Fixed, Paraffin-Embedded Tissue Specimens for the Identification of Novel Substrates Zon W. Lai and Oliver Schilling 9. Identification of Carboxypeptidase Substrates by C-Terminal COFRADIC Sebastian Tanco, Francesc Xavier Aviles, Kris Gevaert, Julia Lorenzo, and Petra Van Damme 10. ProC-TEL: Profiling of Protein C-Termini by Enzymatic Labeling Wenwen Duan and Guoqiang Xu 11. Determining Protease Substrates within a Complex Protein Background using the PROtein TOpography and Migration Analysis Platform (PROTOMAP) R.A. Fuhrman-Luck, L.M. Silva, M.L. Hastie, J.J. Gorman, J.A. Clements 12. Multiplexed Protease Specificity Profiling Using Isobaric Labeling Joanna Tucher and Andreas Tholey 13. FPPS - Fast Profiling of Protease Specificity Matej Vizovisek, Robert Vidmar, and Marko Fonovic 14. Profiling of Protease Cleavage Sites by Proteome-Derived Peptide Libraries and Quantitative Proteomics Chia-yi Chen, Bettina Mayer, and Oliver Schilling 15. Prediction of Proteases Involved in Peptide Generation Mercedes Arguello Casteleiro, Robert Stevens, and Julie Klein 16. Live-Cell Imaging of Protease Activity: Assays to Screen Therapeutic Approaches Anita Chalasani, Kyungmin Ji, Mansoureh Sameni, Samia H. Mazumder, Yong Xu, Kamiar Moin, and Bonnie F. Sloane 17. Protein Translocation Assays to Probe Protease Function and Screen for Inhibitors Angelina Hahlbrock, Dorothйe Gц wein, and Roland H. Stauber 18. Simultaneous Detection of Metalloprotease Activities in Complex Biological Samples Using the PrAMA (Proteolytic Activity Matrix Assay) Method Catharina Conrad, Miles A. Miller, Jцrg W Bartsch, Uwe Schlomann, Douglas A. Lauffenburger 19. Synthesis and Application of Activity-Based Probes for Proteases Tim Van Kersavond, Minh T. N. Nguyen, and Steven H. L. Verhelst
Автор: Schueler-Furman, Ora;London, Nir Название: Modeling Peptide-Protein Interactions : Methods and Protocols ISBN: 1493967967 ISBN-13(EAN): 9781493967964 Издательство: Springer Рейтинг: Цена: 13974.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume covers an array of techniques available for studying peptide-protein docking and design.
Автор: Laure Journet; Eric Cascales Название: Bacterial Protein Secretion Systems ISBN: 1493970313 ISBN-13(EAN): 9781493970315 Издательство: Springer Рейтинг: Цена: 27950.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: 1. Identification of Protein Secretion Systems in Bacterial Genomes using MacSyFinder
Sophie S Abby and Eduardo PC Rocha
2. Protein Sorting Prediction
Henrik Nielsen
3. Cell Fractionation
Melissa Petiti, Laetitia Houot, and Denis Duchй
4. Defining Lipoprotein Localisation by Fluorescence Microscopy
Maria Guillermina Casabona, Mylиne Robert-Genthon, Didier Grunwald, and Ina Attrйe
5. Components Sub-cellular Localization: Identification of Lipoproteins using Globomycin and Radioactive Palmitate
Nienke Buddelmeijer
6. Defining Membrane Protein Localization by Isopycnic Density Gradients
22. Assessing Energy-dependent Protein Conformational Changes in the TonB System
Ray A. Larsen
23. Defining Assembly Pathways by Fluorescence Microscopy
Abdelrahim Zouedand Andreas Diepold
24. Large Complexes: Cloning Strategy, Production, and Purification
Eric Durand and Roland Lloubes
25. Shearing and Enrichment of Extracellular Type IV Pili
Alba Katiria, Gonzalez Rivera, and Katrina T. Forest
26. Blue Native PAGE Analysis of Bacterial Secretion Complexes
Susann Zilkenat, Tobias Dietsche, Julia V. Monjarбs Feria, Claudia E. Torres-Vargas, Mehari Tesfazgi Mebrhatu, and Samuel Wagner
27. In situ Imaging of Bacterial Secretion Systems by Electron Cryo-tomography
Gregor L. Weiss, Joгo M. Medeiros, and Martin Pilhofer
28. Structural Analysis of Protein Complexes by Cryo Electron Microscopy
Tiago R. D. Costa, Athanasios Ignatiou, and Elena V. Orlova
29. Bacterial Filamentous Appendages I
Автор: Wlodawer Alexander, Dauter Zbigniew, Jaskolski Mariusz Название: Protein Crystallography: Methods and Protocols ISBN: 1493983636 ISBN-13(EAN): 9781493983636 Издательство: Springer Рейтинг: Цена: 25155.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
1. Expression and Purification of Recombinant Proteins in Escherichia Coli with a His6 or Dual His6-MBP Tag
Sreejith Raran-Kurussi and David S. Waugh
2. Protein Crystallization
Alexander McPherson
3. Advanced Methods of Protein Crystallization
Abel Moreno
4. The 'Sticky Patch' Model of Crystallization and Modification of Proteins for Enhanced Crystallizability
Zygmunt S. Derewenda and Adam Godzik
5. Crystallization of Membrane Proteins: and Overview
Andrii Ishchenko, Enrique Abola, and Vadim Cherezov
6. Locating and Visualizing Crystals for X-ray Diffraction Experiments
Michael Becker, David J. Kissick, and Craig M. Ogata
7. Collection of X-ray Diffraction Data from Macromolecular Crystals
Zbigniew Dauter
8. Identifying and Overcoming Crystal Pathologies: Disorder and Twinning
Michael C. Thompson
9. Applications of X-ray Micro-beam for Data Collection
Ruslan Sanishvili and Robert F. Fischetti
10. Serial Synchrotron X-ray Crystallography (SSX)
Kay Diederichs and Meitian Wang
11. Time-Resolved Macromolecular Crystallography at Modern X-ray Sources
Marius Schmidt
12. Structure Determination Using X-ray Free-electron Laser Pulses
Henry N. Chapman
13. Processing of XFEL Data
Thomas A. White
14. Many Ways to Derivatize Macromolecules and their Crystals for Phasing
Miroslawa Dauter and Zbigniew Dauter
15. Experimental Phasing: Substructure Solution and Density Modification as Implemented in SHELX
Andrea Thorn
16. Contemporary Use of Anomalous Diffraction in Biomolecular Structure Analysis
Qun Liu and Wayne A. Hendrickson
17. Long-Wavelength X-ray Diffraction and its Applications in Macromolecular Crystallography
Manfred S. Weiss
18. Acknowledging Errors: Advanced Molecular Replacement with Phaser
Airlie J. McCoy
19. Rosetta Structure Prediction as a Tool for Solving Difficult Molecular Replacement Problems
Frank DiMaio
20. Radiation Damage in Macromolecular Crystallography
Elspeth F. Garman and Martin Weik
21. Boxes of Model Building and Visualization
Dusan Turk
22. Structure Refinement at Atomic Resolution
Mariusz Jaskolski
23. Low Resolution Refinement of Atomic Models against Crystallographic Data
Robert A. Nicholls, Oleg Kovalevskiy, and Garib N. Murshudov
24. Stereochemistry and Validation of Macromolecular Structures
Alexander Wlodawer
25. Validation of Protein-Ligand Crystal Structure Models: Small Molecule and Peptide Ligands
Edwin Pozharski, Marc C. Deller and Bernhard Rupp
26. Protein Data Bank - the Single Global Macromolecular Structure Archive Managed by the Worldwide Protein Data Bank
Stephen K. Burley Helen M. Berman, Gerard J. Kleywegt, John L. Markley, Haruki Nakamura, and Sameer Velankar
27. Databases, Repositories and Other Data Resources in Structural Biology
Heping Zheng, Przemyslaw J. Porebski, Marek Grabowski, David R. Cooper, and Wladek Minor
Автор: Bhupendra Pushkar Название: Protein Synthesis: Methods and Protocols ISBN: 1774077035 ISBN-13(EAN): 9781774077030 Издательство: Mare Nostrum (Eurospan) Рейтинг: Цена: 22730.00 р. Наличие на складе: Нет в наличии.
Описание: Takes readers through the various ways in which proteins are synthesized and then further used for other applications. The book throws light on chemical reactions taking place in the process, and lays down different kinds of methods and the procedures that are followed in protein synthesis.
Автор: Kihara Daisuke Название: Protein Function Prediction: Methods and Protocols ISBN: 1493983687 ISBN-13(EAN): 9781493983681 Издательство: Springer Рейтинг: Цена: 19564.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Preface... Table of Contents... Contributing Authors... 1. Using PFP and ESG Protein Function Prediction Web Servers Qing Wei, Joshua McGraw, Ishita Khan, and Daisuke Kihara 2. GHOSTX: A Fast Sequence Homology Search Tool for Functional Annotation of Metagenomic Data Shuji Suzuki, Takashi Ishida, Masahito Ohue, Masanori Kakuta, and Yutaka Akiyama 3. From Gene Annotation to Function Prediction for Metagenomics Fatemeh Sharifi and Yuzhen Ye 4. An Agile Functional Analysis of Metagenomic Data using SUPER-FOCUS Genivaldo Gueiros Z. Silva, Fabyano A. C. Lopes, and Robert A. Edwards 5. MPFit: Computational Tool for Predicting Moonlighting Proteins Ishita Khan, Joshua McGraw, and Daisuke Kihara 6. Predicting Secretory Proteins with SignalP Henrik Nielsen 7. The ProFunc Function Prediction Server Roman A. Laskowski 8. G-LoSA for Prediction of Protein-Ligand Binding Sites and Structures Hui Sun Lee andWonpil Im 9. Local Alignment of Ligand Binding Sites in Proteins for Polypharmacology and Drug Repositioning Michal Brylinski 10. WATsite2.0 with PyMOL Plugin: Hydration Site Prediction and Visualization Ying Yang, Bingjie Hu, and Markus A. Lill 11. Enzyme Annotation and Metabolic Reconstruction Using KEGG Minoru Kanehisa 12. Ortholog Identification and Comparative Analysis of Microbial Genomes using MBGD and RECOG Ikuo Uchiyama 13. Exploring Protein Function Using the Saccharomyces Genome Database Edith D. Wong 14. Network-Based Gene Function Prediction in Mouse and Other Model Vertebrates using MouseNet Server Eiru Kim and Insuk Lee 15. The FANTOM5 Computation Ecosystem: Genomic Information Hub for Promoters and Active Enhancers Imad Abugessaisa, Shuhei Noguchi, Piero Carninci, and Takeya Kasukawa 16. Multi-Algorithm Particle Simulations with Spatiocyte Satya N. V. Arjunan and Koichi Takahashi
Описание: In this present volume, different approaches are detailed to produce membrane proteins, purify them, study their function, determine their structure, and model them in membrane.
Автор: Walls Dermot, Loughran Sinйad T. Название: Protein Chromatography: Methods and Protocols ISBN: 1493961292 ISBN-13(EAN): 9781493961290 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: A prerequisite for elucidating the structure and function of any protein is the prior purification of that protein. This necessity has led to the development of many purification schemes and chromatographic methods for the isolation of native proteins from complex sources. In Protein Chromatography: Methods and Protocols, leading researchers present clear protocol-style chapters that are suitable for newcomers and experts alike. The book opens with vital topics in protein biochemistry, addressing such areas as protein stability and storage, avoiding proteolysis during chromatography, protein quantitation methods including immuno-qPCR, and the contrasting challenges that microfluidics and scale-up production pose to the investigator, and then it segues into key methods involving the generation and purification of recombinant proteins through recombinant antibody production and the tagging of proteins, amongst other means, as well as many variations on classic techniques such as ion-exchange and immunoaffinity chromatography. Written in the highly successful Methods in Molecular Biology™ series format, protocols chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Protein Chromatography: Methods and Protocols will greatly aid scientists in establishing these essential techniques in their own laboratories and furthering our understanding of the many imperative functions of proteins.
Описание: This volume discusses protocols that cover genetic manipulation of Chinese hamster ovary (CHO) cells for recombinant protein production, and protocols for the characterization of CHO cells using ‘omic approaches. This book also explores methods that discuss the genome editing tool, CRISPR/Cas9, and the characterization of recombinant protein products, such as glycosylation and host cell protein analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Heterologous Protein Production in CHO Cells: Methods and Protocols is a valuable resource for scientists and researchers who are interested in further studying cell production in CHO cells.
Автор: Kister Alexander E. Название: Protein Supersecondary Structures: Methods and Protocols ISBN: 1493991604 ISBN-13(EAN): 9781493991600 Издательство: Springer Рейтинг: Цена: 20962.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
This second edition volume expands on the previous edition with an update on the latest developments in the field and new techniques used to study secondary and supersecondary structures (SSS) in proteins. Chapters in this book discuss topics such as sequence and structural features of different SSS elements; software used for the automatic annotation of secondary structure elements in proteins; the new developments in secondary and SSS prediction and the modern approaches for energy landscape calculation; protein misfolding and amyloid structural formation; analysis of ‘transformer proteins’; discovery of the structure of the hydrophobic nucleus; and discussions of the main principles of protein structures formation. The contributing authors of the volume are the eminent experts in the field of protein research and bioinformatics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and authoritative, Protein Supersecondary Structures: Methods and Protocols, Second Edition is a valuable resource for researchers who are interested in learning more about the relationship between amino acids sequences and protein structures, the evolution of proteins and the dynamics of protein formation.
Автор: Pulido Rafael Название: Protein Tyrosine Phosphatases: Methods and Protocols ISBN: 1493981277 ISBN-13(EAN): 9781493981274 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This book provides coverage, methodology, and laboratory protocols on the more essential aspects of protein tyrosine phosphatase (PTP) function and regulation, including the use of standardized in vitro functional assays, suitable cell systems, and animal and microorganism models. Chapters covering state-of-the-art technical approaches suitable to decipher the physiologic roles of PTPs, and their involvement in tissue-specific functions, are also included, which will be of utility for both newcomers and experienced researchers in the field of tyrosine- and phosphoinositide- phosphorylation/dephosphorylation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Protein Tyrosine Phosphatases: Methods and Protocols aims to aid researchers in better defining the common and individual features of the PTP family members and translating this knowledge into PTP-based therapy for human disease.
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