Microbial Steroids: Methods and Protocols, Barredo Josй-Luis, Herrбiz Ignacio

Автор: Jiaju Zhou
Название: Polyketides and Steroids
ISBN: 311065363X ISBN-13(EAN): 9783110653632
Издательство: Walter de Gruyter
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Описание: This 8-volume set provides a systematic description on 8,350 active marine natural products from 3,025 various kinds of marine organisms. The diversity of structures, biological resources and pharmacological activities are discussed in detail. Molecular structural classification system with 264 structural types are developed as well. The 5th volume mainly illustrates the molecular formula and structures of polyketides and steroids.

Автор: GГјbitz Gerald, Schmid Martin G.
Название: Chiral Separations / Methods and Protocols
ISBN: 1588291502 ISBN-13(EAN): 9781588291509
Издательство: Springer
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Описание: Prominent experts from around the world detail the chromatographic and electroseparation techniques they have developed for chiral separations on an analytical scale. Described in step-by-step detail to ensure successful experimental results, the procedures are presented as either general methods or as specific applications to substance classes and special compounds, with emphasis on high performance liquid chromatography and capillary electrophoresis techniques, but also including thin layer chromotographic, gas chromatographic, supercritical fluid chromatographic as well as recent electrochromotographic techniques.

Автор: Lorette C. Javois
Название: Immunocytochemical Methods and Protocols
ISBN: 1617370789 ISBN-13(EAN): 9781617370786
Издательство: Springer
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Описание: Lorette Javois` timely new 2nd edition revises and updates her widely acclaimed collection of step-by-step immunocytochemical methods, one that is now used in many biological and biomedical research programs.

Автор: Moulton Hong M., Moulton Jon D.
Название: Morpholino Oligomers: Methods and Protocols
ISBN: 1493983075 ISBN-13(EAN): 9781493983070
Издательство: Springer
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Описание:

Invention and Early History of Morpholinos: From Pipe Dream to Practical Products.- Making a Morpholino Experiment Work: Controls, Favoring Specificity, Improving Efficacy, Storage, and Dose.- Aggregation and Disaggregation of Morpholino Oligomers in Solution.- End-Modifications on Morpholino Oligos.- Inducible Inhibition of Gene Function with Photomorpholinos.- Blocking Zebrafish microRNAs with Morpholinos.- Using Morpholinos to Examine Gene Function During Fin Regeneration.- Using Morpholinos to Probe Gene Networks in Sea Urchin.- Manipulating Gene Expression in the Chick Embryo.- Inhibition of Bacterial Growth by Peptide-conjugated Morpholino Oligomers.- Use of Translation Blocking Morpholinos for Gene Knock-Down in Giardia lamblia.- Regulation of Isoform Expression by Blocking Polyadenylation Signal Sequences with Morpholinos.- Targeting Functional Non-coding RNAs.- Use of Morpholino Oligomers for Pretargeting.- Diagnostic Applications of Morpholinos and Label-free Electrochemical Detection of Nucleic Acids.- Intranasal Delivery of Peptide-Morpholinos to Knockdown Influenza Host Factors in Mice.- Systemic Delivery of Morpholinos to Skip Multiple Exons in a Dog Model of Duchenne Muscular Dystrophy.- In vivo and Explant Electroporation of Morpholinos in the Developing Mouse Retina.

Автор: M. Capek; O. Hanc; M. Tadra
Название: Microbial Transformations of Steroids
ISBN: 9401176051 ISBN-13(EAN): 9789401176057
Издательство: Springer
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Описание: Fundamental studies of steroid compounds have begun approximately thirty years ago with the solution of structures of cholesterol and bile acids. This field has been receiving more attention when the structures of the principal steroid hormones, aglycones of heart glycosides, sapogenins, steroid alkaloids, toad poisons have been established. An intensive interest in these derivatives of cyc1opentanoperhydrophe­ nanthrene was determined especially by practical viewpoints, notably by attempts to prepare by synthetical economical procedures a series of steroid hormones and their biologically active derivatives, further by theoretical viewpoints. Biochemistry of steroids was developing in its beginnings by studies of meta­ bolites of steroid hormones in animal organism and soon has brought a number of practical results that could be used also in medical diagnostics. Considerably later, the metabolism of steroid compounds was followed also in microorganisms, again in connection with the practical problem to aid to economical syntheses of some steroid hormones. Two names are of primary importance for the pioneer works in microbial transformations of steroids, that of the Italian worker L. MAMOLI who first realized the microbial biosynthesis of testosterone from dehydroepiandro­ sterone, and of the American chemist and microbiologist D. H. PETERSON, who has contributed by his fundamental experiments in microbial hydroxylation of steroids to the position eleven, especially to the synthesis of cortisone. Microbial transforma­ tions of steroids nowadays offer a valuable tool in the preparation of series otherwise difficultly accessible steroid compounds.

Автор: Jos?-Luis Barredo; Ignacio Herr?iz
Название: Microbial Steroids
ISBN: 1493971824 ISBN-13(EAN): 9781493971824
Издательство: Springer
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Описание:

Preface...
Table of Contents...
Contributing Authors...

1. Steroid Bioconversions
Marina V. Donova

2. Chemical Pathways of Corticosteroids, Industrial Synthesis from Sapogenins
Ignacio Herrбiz

3. Genome Sequencing of Steroid Producing Bacteria with Illumina Technology
Victoria Y. Shtratnikova, Mikhail I. Schelkunov, and marina V. Donova

4. Genome Sequencing of Steroid Producing Bacteria using Ion Torrent Technology and a Reference Genome
Alberto Sola-Landa, Antonio Rodrнguez-Garcнa, Carlos Barreiro, and Rosario Pйrez-Redondo

5. RNA-Seq-Based Comparative Transcriptomics: RNA Preparation and Bioinformatics
Antonio Rodrнguez-Garcнa, Alberto Sola-Landa, and Carlos Barreiro

6. Intra- and Extra-Cellular Proteome Analyses of Steroid-Producer Mycobacteria
Carlos Barreiro, Alejandro Morales, Inйs Vбzquez-Iglesias, and Alberto Sola-Landa

7. Genetic Techniques for Manipulation of the Phytosterol Biotransformation Strain Mycobacteriumneoaurum NRRL B-3805
Jessica K. Loraine and Margaret C. M. Smith

8. Identification and Characterization of the genes and Enzymes Belonging to the Bile Acid Catabolic Pathway in Pseudomonas
Josй M. Luengo and Elias R. Olivera

9. Selection of Biodegrading Phytosterol Strains
Marнa-Angйlica Mondaca, Maricel Vidal, Soledad Chamorro, and Gladys Vidal

10. Stigmasterol Removal by an Aerobic Treatment System
Soledad Chamorro, Claudia Xavier, Vнctor Hernбndez, Josй Becerra, and Gladys Vidal

11. Production and Biotransformation of Phytosterol Microdispersions to Produce 4-Androstene-3, 17-Dione
Rodrigo A. Mancilla, Roberto Pavez-Dнaz, and Andrea Muсoz-Orrego

12. Β-Sitosterol Bioconversion to Androstenedione in Microtiter Plates
Marco P.C. Marques and Pedro Fernandez

13. Bioconversion of Phytosterols into Androstenedione by Mycobacterium
Kjell D. Josefsen, Anna Nordborg, and Hеvard Sletta

14. Scale-Up of Phytosterols Bioconversion into Androstenedione
Sonia Martнnez-Cбmara, Esther Bahнllo, Josй-Luis Barredo, and Marta Rodrнguez-Sбiz

15. Bioconversion of Phytosterols into Androstadienedione by Mycobacterium smegmatis CECT 8331
Julia Garcнa-Fernбndez, Igor Martнnez, Lorena Fernбndez-Cabezуn, Carmen Felpeto-Santero, Josй-Luis Garcнa, and Beatriz Galбn

16. Process for Biotransformation of Androsta-4-ene-3, 17-Dione (4-AD) to Androsta-1, 4-Diene-3, 17-Dione (ADD)
Surya Prakash and Abhay Bajaj

17. Laboratory-Scale Hydroxylation of Steroids by P450_BM3 Variants
Sabrina Hoebenreich, Martin Spinck, and Nathalie Nett

18. Obtaining of 11α-Hydroxy and Rost-4-ene-3, 17-Dione from Natural Sterols
Dmitry Dovbnya, Sergey Khomutov, Vyacheslav Kollerov, Marina Donova

19. Steroid 11-Alfa-Hydroxylation by the Fungi Aspergillus nidulans and Aspergillus ochraceus
Lidia Ortega-de los Rнos, Josй M. Luengo, and Josй M. Fernбndez-Caсуn

20. Biotransformation of DHEA into 7α, 15α-diOH-DHEA
Hui Li, Jin Sun, and Zhenghong Xu

21. Stabilization of Enzymes by Using Thermophiles
Ana-Luisa Ribeiro, Mercedes Sбnchez, Aurelio Hidalgo, and Josй Berenguer

22. Biocatalysis of Steroids with Mycobacterium Sp. in Aqueous and Organic Media
Carla C. C. R. de Carvalho and Pedro Fernandes

23. Downstream Process Synthesis for Microbial Steroids
Fabian B. Thygs and Juliane Merz

24. Analysis of Intermediates of Steroid Transformations in Resting Cells by Thin Layer Chromatography (TLC)
Govinda Guevara, Juliбn Perera, and Juana-Marнa Navarro-Llorens

Автор: Bhattacharya Sanjoy K.
Название: Lipidomics: Methods and Protocols
ISBN: 1493983628 ISBN-13(EAN): 9781493983629
Издательство: Springer
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Описание:

Preface...
Table of Contents...
Contributing Authors...

1. Lipid Sample Preparation for Biomedical Research
Ravin Sajnani and Katyayini Aribindi

2. Lipid Extraction Techniques for Stable Isotope Analysis and Ecological Assays
Kyle H. Elliot, James D. Roth, and Kevin Crook

3. Isolation of Lipid Raft Proteins from CD133+ Cancer Stem Cells
Vineet K. Gupta and Sulagna Banerjee

4. Isolation of Neuronal Synaptic Membranes by Sucrose Gradient Centrifugation
Blake E. Hopiavuori, Dustin E. Masser, Joseph L. Wilkerson, Richard S. Brush, Nawajes A. Mandal, Robert E. Anderson, and Willard M. Freeman

5. Sample Preparation and Analysis for Imaging Mass Spectrometry
Genea Edwards, Annia Mesa, Robert I. Vazquez-Padron, Paul Kowalski, and Sanjoy K. Bhattacharya

6. Direct Measurement of Free and Esterified Cholesterol Mass in Differentiated Human Podocytes: A TLC and Enzymatic Assay Based Method
Christopher E. Pedigo, Sandra M. Merscher, and Alessia Fornoni

7. High-Performance Chromatographic Separation of Cerebrosides
Renaud Sicard and Ralf Landgraf

8. Lipid Identification by Untargeted Tandem Mass Spectrometry Coupled with Ultra-High-Pressure Liquid Chromatography
Gabriel B. Gugiu

9. Utility of Moderate and High Resolution Mass Spectrometry for Class-Specific Lipid Identification and Quantification
Maria del Carmen Piqueras

10. A Robust Lipidomics Workflow for Mammalian Cells, Plasma, and Tissue using Liquid-Chromatography High-Resolution Tandem Mass Spectrometry
Candice Z. Ulmer, Rainey Patterson, Jeremy Koelmel, Timothy J. Garrett, and Richard A. Yost

11. Combined Use of MALDI-TOF Mass Spectrometry and ³¹PNMR Spectrometry for Analysis of Phospholipids
Jenny Schrцter, Yulia Popkova, Rosmarie Sь , and Jьrgen Schiller

12. Global Monitoring of Mammalian Lipidome by Quantitative Shotgun Lipodomics
Inger dum Nielsen, Kenji Maeda, and Mesut Bilgin

13. Bioinformatics Pertinent to Lipid Analysis in Biological Samples
Justin Ma, Ulises Arbelo, Yenifer Guerra, Katyayini Aribindi, Sanjoy K. Bhattacharya, and Daniel Pelaez

14. LC-MS-Based Lipidomics and Automated Identification of Lipids using the LipidBlast In-Silico MS/MS Library
Tomas Cajka and Oliver Fiehn

15. Single-Step Capture and Targeted Metabolomics of Alkyl-Quinolones in Outer Membrane Vesicles of Pseudomonas aeruginosa
Pallavi Lahiri and Dipankar Ghosh

16. Analysis of Fatty Acid and Cholesterol Content from Detergent-Resistant and Detergent-Free Membrane Microdomains
Mark E. McClellan and Michael H. Elliott

17. Computational Functional Analysis of Lipid Metabolic Enzymes
Carolina Bagnato, Arjen Ten Have, Marнa B. Prados, and Marнa B. Beligni

18. Isoprenylation of Monomeric GTPases in Human Trabecular Meshwork Cells
Evan B. Stubbs, Jr.

19. Purification and Validation of Lipid Transfer Proteins
Matti A. Kjellberg, Anders P.E. Backman, Anna Mцuts, and Peter Mattjus

20. Incorporation of Artificial Lipid-Anchored Proteins into Cultured Mammalian Cells
Rania Leventis and John R. Silvius

21. Sonication-Based Basic Protocol for Liposome Synthesis
Roberto Mendez and Santanu Banerjee

22. On Electrochemical Methods for Determination of Protein-Lipid Interaction
Zhiping Hu and Yanli Mao

23. Angiogenesis Model of Cornea to Understand the Role of Sphingosine 1-Phosphate
Joseph L. Wilkerson and Nawajes A. Mandal

Автор: Wilkesman Jeff, Kurz Liliana
Название: Zymography: Methods and Protocols
ISBN: 1493983873 ISBN-13(EAN): 9781493983872
Издательство: Springer
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Описание: This detailed volume examines the latest techniques and protocols associated with zymography, the practice of detecting enzyme activity on electrophoretic gels. Sections cover subjects such as endopeptidase zymography, reverse zymography & in situ zymography, 2D zymography, as well as a variety of special cases.

Автор: Espada Jesъs
Название: Reactive Oxygen Species: Methods and Protocols
ISBN: 1071608959 ISBN-13(EAN): 9781071608951
Издательство: Springer
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Описание: This volume provides a varied selection of current methods and molecular probes used to generate, image and quantify ROS production and lipid peroxidation. Chapters guide readers through methods and protocols on in silico modelling of ROS dynamics in biological systems and a breaking proposal. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Reactive Oxygen Species: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Автор: Yung-I Lee; Edward Chee-Tak Yeung
Название: Orchid Propagation: From Laboratories to Greenhouses—Methods and Protocols
ISBN: 1493992805 ISBN-13(EAN): 9781493992805
Издательство: Springer
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Описание: The orchid family is one of the largest families of flowering plants known for their beauty and economic importance. This work provides information in key areas of research that are important to both scientists and commercial growers alike. The main purposes of this book are to provide key practical areas of research, such as, germination, micropropagation, traditional and current techniques related to plant improvement; document methods that ensure survival of plants from laboratories to greenhouses; promote communication between scientists and growers, so that their combined expertise on these areas will lead to the successful growth of orchids in their natural habitats or commercial greenhouses. This book can serve as reference for laymen with an interest in orchid growing.