Zymography: Methods and Protocols, Wilkesman Jeff, Kurz Liliana
Автор: Jeff Wilkesman; Liliana Kurz Название: Zymography ISBN: 1493971093 ISBN-13(EAN): 9781493971091 Издательство: Springer Рейтинг: Цена: 20962.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This detailed volume examines the latest techniques and protocols associated with zymography, the practice of detecting enzyme activity on electrophoretic gels. Sections cover subjects such as endopeptidase zymography, reverse zymography & in situ zymography, 2D zymography, as well as a variety of special cases.
Автор: GГјbitz Gerald, Schmid Martin G. Название: Chiral Separations / Methods and Protocols ISBN: 1588291502 ISBN-13(EAN): 9781588291509 Издательство: Springer Рейтинг: Цена: 21661.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Prominent experts from around the world detail the chromatographic and electroseparation techniques they have developed for chiral separations on an analytical scale. Described in step-by-step detail to ensure successful experimental results, the procedures are presented as either general methods or as specific applications to substance classes and special compounds, with emphasis on high performance liquid chromatography and capillary electrophoresis techniques, but also including thin layer chromotographic, gas chromatographic, supercritical fluid chromatographic as well as recent electrochromotographic techniques.
Автор: Bailon Pascal Название: Affinity Chromatography / Methods and Protocols ISBN: 0896036944 ISBN-13(EAN): 9780896036949 Издательство: Springer Цена: 7540.00 р. Наличие на складе: Поставка под заказ.
Автор: Galea Charles A. Название: Matrix Metalloproteases: Methods and Protocols ISBN: 1493983210 ISBN-13(EAN): 9781493983216 Издательство: Springer Рейтинг: Цена: 16070.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
PART I Expression and Purification of Matrix Metalloproteases
1. Expression and Purification of Matrix Metalloproteinases in Escherichia coli
Krishna K. Singh, Ruchi Jain, Harini Ramanan, and Deepak K. Saini
2. Expression and Purification of a Matrix Metalloprotease Transmembrane Domain in Escherichia coli
Charles A. Galea
3. Heterologous Expression of the Astacin Protease Meprin b in Pichia pastoris
Dagmar Schlenzig and Stephan Schilling
PART II Structural Characterization of Matrix Metalloproteases
4. Structural Studies of Matrix Metalloproteinase by X-Ray Diffraction
Elena Decaneto, Wolfgang Lubitz, and Hideaki Ogata
5. Mapping Lipid Bilayer Recognition Sites of Metalloproteinases and other Prospective Peripheral Membrane Proteins
Tara C. Marcink, Rama K. Koppisetti, Yan G. Fulcher, and Steven R. Van Doren
6. Using Small Angle X-ray Scattering (SAXS) to Characterise the Solution Conformation and Flexibility of Matrix Metalloproteinases (MMPs)
Louise E. Butt, Robert A. Holland, Nikul S. Khunti, Debra L. Quinn, and Andrew R. Pickford
PART III Computational Simulations of Matrix Metalloproteases
7. Molecular Dynamics Studies of Matrix Metalloproteases
Natalia Dнaz and Dimas Suбrez
PART IV Determining Matrix Metalloprotease Substrate Specificity
8. Determining the Substrate Specificity of Matrix Metalloproteases using Fluorogenic Peptide Substrates
Maciej J. Stawikowski, Anna M. Knapinska, and Gregg B. Fields
9. Time-resolved Analysis of Matrix Metalloproteinase Substrates in Complex Samples
Pascal Schlage, Fabian E. Egli, and Ulrich auf dem Keller
10. Identification of Protease Cleavage Sites by Charge-Based Enrichment of Protein N-termini
Zon W. Lai and Oliver Schilling
11. Mapping the Substrate Recognition Landscapes of Metalloproteases using Comprehensive Mutagenesis
Colin A. Kretz
PART V Detection of Matrix Metalloproteases
12. Detection of Matrix Metalloproteinases by Zymography
Rajeev B. Tajhya, Rutvik S. Patel, and Christine Beeton
13. Imaging Matrix Metalloproteases in Spontaneous Colon Tumors: Validation by Correlation with Histopathology
Harvey Hensley, Harry S. Cooper, Wen-Chi L. Chang, and Margie L. Clapper
PART VI Matrix Metalloprotease Inhibitors 14. Virtual High-throughput Screening for Matrix Metalloproteinase Inhibitors Jun Yong Choi, and Rita Fuerst 15. Computational Approaches to Matrix Metalloprotease Drug Design
Tanya Singh, B. Jayaram, and Olayiwola Adedotun Adekoya
16. A Simple, Adaptable Blood-Brain Barrier Cell Model for Screening Matrix Metalloproteinase Inhibitor Functionality
Jennifer S. Myers, Joan Hare, and Qing-Xiang Amy Sang
PART VII Matrix Metalloproteases as Biomarkers
17. Matrix Metalloproteases as Biomarkers of Disease
Fernando Luiz Affonso Fonseca, Beatriz da Costa Aguiar Alves, Ligia Ajaime Azzalis, and Thaнs Moura Gбscon Belardo
Автор: Simpson Richard J., Greening David W. Название: Serum/Plasma Proteomics: Methods and Protocols ISBN: 1493958011 ISBN-13(EAN): 9781493958016 Издательство: Springer Рейтинг: Цена: 20263.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Blood science has become a cornerstone of multiple disciplines, including clinical chemistry, disease diagnosis, and therapeutic monitoring. Over the past decade, we have witnessed the advent of increasingly powerful proteomics technologies that allow greater fundamental insights into the blood proteome. These technological improvements have, in part, fuelled the quest for the discovery of novel blood-based biomarkers of disease. Serum/Plasma Proteomics: Methods and Protocols is a comprehensive resource of protocols for areas, pre-analytical through to analytical, of plasma and serum proteomics. Divided into five convenient sections, this detailed volume covers fractionation strategies for in-depth blood proteome analysis, defined procedures for blood collection, handling and storage, detailed protocols for performing both antibody-based and non-antibody based quantitative assays, proteome analysis of blood cell compartments, circulating nanomebraneous vesicles and blood-related fluids, and finally data management, statistical design, and bioinformatic challenges. This book, contributed to by leading experts in the field, provides a valuable foundation for the development and application of blood-based proteomics. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Serum/Plasma Proteomics: Methods and Protocols, with its well-honed methodologies, seeks to serve both professionals and investigators new to the field in an effort to further our knowledge of this fundamental science.
Автор: Bhupendra Pushkar Название: Protein Synthesis: Methods and Protocols ISBN: 1774077035 ISBN-13(EAN): 9781774077030 Издательство: Mare Nostrum (Eurospan) Рейтинг: Цена: 22730.00 р. Наличие на складе: Нет в наличии.
Описание: Takes readers through the various ways in which proteins are synthesized and then further used for other applications. The book throws light on chemical reactions taking place in the process, and lays down different kinds of methods and the procedures that are followed in protein synthesis.
Автор: Bhattacharya Sanjoy K. Название: Lipidomics: Methods and Protocols ISBN: 1493983628 ISBN-13(EAN): 9781493983629 Издательство: Springer Рейтинг: Цена: 19564.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
Preface... Table of Contents... Contributing Authors...
1. Lipid Sample Preparation for Biomedical Research Ravin Sajnani and Katyayini Aribindi 2. Lipid Extraction Techniques for Stable Isotope Analysis and Ecological Assays Kyle H. Elliot, James D. Roth, and Kevin Crook 3. Isolation of Lipid Raft Proteins from CD133+ Cancer Stem Cells Vineet K. Gupta and Sulagna Banerjee 4. Isolation of Neuronal Synaptic Membranes by Sucrose Gradient Centrifugation Blake E. Hopiavuori, Dustin E. Masser, Joseph L. Wilkerson, Richard S. Brush, Nawajes A. Mandal, Robert E. Anderson, and Willard M. Freeman 5. Sample Preparation and Analysis for Imaging Mass Spectrometry Genea Edwards, Annia Mesa, Robert I. Vazquez-Padron, Paul Kowalski, and Sanjoy K. Bhattacharya 6. Direct Measurement of Free and Esterified Cholesterol Mass in Differentiated Human Podocytes: A TLC and Enzymatic Assay Based Method Christopher E. Pedigo, Sandra M. Merscher, and Alessia Fornoni 7. High-Performance Chromatographic Separation of Cerebrosides Renaud Sicard and Ralf Landgraf 8. Lipid Identification by Untargeted Tandem Mass Spectrometry Coupled with Ultra-High-Pressure Liquid Chromatography Gabriel B. Gugiu 9. Utility of Moderate and High Resolution Mass Spectrometry for Class-Specific Lipid Identification and Quantification Maria del Carmen Piqueras 10. A Robust Lipidomics Workflow for Mammalian Cells, Plasma, and Tissue using Liquid-Chromatography High-Resolution Tandem Mass Spectrometry Candice Z. Ulmer, Rainey Patterson, Jeremy Koelmel, Timothy J. Garrett, and Richard A. Yost 11. Combined Use of MALDI-TOF Mass Spectrometry and 31PNMR Spectrometry for Analysis of Phospholipids Jenny Schrцter, Yulia Popkova, Rosmarie Sь , and Jьrgen Schiller 12. Global Monitoring of Mammalian Lipidome by Quantitative Shotgun Lipodomics Inger dum Nielsen, Kenji Maeda, and Mesut Bilgin13. Bioinformatics Pertinent to Lipid Analysis in Biological Samples Justin Ma, Ulises Arbelo, Yenifer Guerra, Katyayini Aribindi, Sanjoy K. Bhattacharya, and Daniel Pelaez 14. LC-MS-Based Lipidomics and Automated Identification of Lipids using the LipidBlast In-Silico MS/MS Library Tomas Cajka and Oliver Fiehn 15. Single-Step Capture and Targeted Metabolomics of Alkyl-Quinolones in Outer Membrane Vesicles of Pseudomonas aeruginosa Pallavi Lahiri and Dipankar Ghosh 16. Analysis of Fatty Acid and Cholesterol Content from Detergent-Resistant and Detergent-Free Membrane Microdomains Mark E. McClellan and Michael H. Elliott 17. Computational Functional Analysis of Lipid Metabolic Enzymes Carolina Bagnato, Arjen Ten Have, Marнa B. Prados, and Marнa B. Beligni 18. Isoprenylation of Monomeric GTPases in Human Trabecular Meshwork Cells Evan B. Stubbs, Jr. 19. Purification and Validation of Lipid Transfer Proteins Matti A. Kjellberg, Anders P.E. Backman, Anna Mцuts, and Peter Mattjus 20. Incorporation of Artificial Lipid-Anchored Proteins into Cultured Mammalian Cells Rania Leventis and John R. Silvius 21. Sonication-Based Basic Protocol for Liposome Synthesis Roberto Mendez and Santanu Banerjee 22. On Electrochemical Methods for Determination of Protein-Lipid Interaction Zhiping Hu and Yanli Mao 23. Angiogenesis Model of Cornea to Understand the Role of Sphingosine 1-Phosphate Joseph L. Wilkerson and Nawajes A. Mandal
Автор: Lorette C. Javois Название: Immunocytochemical Methods and Protocols ISBN: 1617370789 ISBN-13(EAN): 9781617370786 Издательство: Springer Рейтинг: Цена: 13409.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Lorette Javois` timely new 2nd edition revises and updates her widely acclaimed collection of step-by-step immunocytochemical methods, one that is now used in many biological and biomedical research programs.
Автор: Moulton Hong M., Moulton Jon D. Название: Morpholino Oligomers: Methods and Protocols ISBN: 1493983075 ISBN-13(EAN): 9781493983070 Издательство: Springer Рейтинг: Цена: 19564.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
Invention and Early History of Morpholinos: From Pipe Dream to Practical Products.- Making a Morpholino Experiment Work: Controls, Favoring Specificity, Improving Efficacy, Storage, and Dose.- Aggregation and Disaggregation of Morpholino Oligomers in Solution.- End-Modifications on Morpholino Oligos.- Inducible Inhibition of Gene Function with Photomorpholinos.- Blocking Zebrafish microRNAs with Morpholinos.- Using Morpholinos to Examine Gene Function During Fin Regeneration.- Using Morpholinos to Probe Gene Networks in Sea Urchin.- Manipulating Gene Expression in the Chick Embryo.- Inhibition of Bacterial Growth by Peptide-conjugated Morpholino Oligomers.- Use of Translation Blocking Morpholinos for Gene Knock-Down in Giardia lamblia.- Regulation of Isoform Expression by Blocking Polyadenylation Signal Sequences with Morpholinos.- Targeting Functional Non-coding RNAs.- Use of Morpholino Oligomers for Pretargeting.- Diagnostic Applications of Morpholinos and Label-free Electrochemical Detection of Nucleic Acids.- Intranasal Delivery of Peptide-Morpholinos to Knockdown Influenza Host Factors in Mice.- Systemic Delivery of Morpholinos to Skip Multiple Exons in a Dog Model of Duchenne Muscular Dystrophy.- In vivo and Explant Electroporation of Morpholinos in the Developing Mouse Retina.
Автор: McGuckin Michael A., Thornton David J. Название: Mucins: Methods and Protocols ISBN: 1493958860 ISBN-13(EAN): 9781493958863 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Epithelial mucins are large complex cell surface and secreted glycoproteins produced by mucosal epithelial cells. In, Mucins: Methods and Protocols expert researchers in the field detail many of the methods which are now commonly used to study Mucins. These include methods and techniques for the best approaches to analysing each specific area of mucin biochemistry, physiology and biophysics before providing individual detailed experimental protocols together with troubleshooting and interpretation tips. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Mucins: Methods and Protocols is designed to be a useful resource for those entering the mucin field and to facilitate those already studying mucins to broaden their experimental approaches to understanding mucosal biology.
Автор: Barredo Josй-Luis, Herrбiz Ignacio Название: Microbial Steroids: Methods and Protocols ISBN: 1493984101 ISBN-13(EAN): 9781493984107 Издательство: Springer Рейтинг: Цена: 16769.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
Preface... Table of Contents... Contributing Authors...
1. Steroid Bioconversions Marina V. Donova 2. Chemical Pathways of Corticosteroids, Industrial Synthesis from Sapogenins Ignacio Herrбiz 3. Genome Sequencing of Steroid Producing Bacteria with Illumina Technology Victoria Y. Shtratnikova, Mikhail I. Schelkunov, and marina V. Donova 4. Genome Sequencing of Steroid Producing Bacteria using Ion Torrent Technology and a Reference Genome Alberto Sola-Landa, Antonio Rodrнguez-Garcнa, Carlos Barreiro, and Rosario Pйrez-Redondo 5. RNA-Seq-Based Comparative Transcriptomics: RNA Preparation and Bioinformatics Antonio Rodrнguez-Garcнa, Alberto Sola-Landa, and Carlos Barreiro 6. Intra- and Extra-Cellular Proteome Analyses of Steroid-Producer Mycobacteria Carlos Barreiro, Alejandro Morales, Inйs Vбzquez-Iglesias, and Alberto Sola-Landa 7. Genetic Techniques for Manipulation of the Phytosterol Biotransformation Strain Mycobacteriumneoaurum NRRL B-3805 Jessica K. Loraine and Margaret C. M. Smith 8. Identification and Characterization of the genes and Enzymes Belonging to the Bile Acid Catabolic Pathway in Pseudomonas Josй M. Luengo and Elias R. Olivera 9. Selection of Biodegrading Phytosterol Strains Marнa-Angйlica Mondaca, Maricel Vidal, Soledad Chamorro, and Gladys Vidal 10. Stigmasterol Removal by an Aerobic Treatment System Soledad Chamorro, Claudia Xavier, Vнctor Hernбndez, Josй Becerra, and Gladys Vidal 11. Production and Biotransformation of Phytosterol Microdispersions to Produce 4-Androstene-3, 17-Dione Rodrigo A. Mancilla, Roberto Pavez-Dнaz, and Andrea Muсoz-Orrego 12. Β-Sitosterol Bioconversion to Androstenedione in Microtiter Plates Marco P.C. Marques and Pedro Fernandez 13. Bioconversion of Phytosterols into Androstenedione by Mycobacterium Kjell D. Josefsen, Anna Nordborg, and Hеvard Sletta 14. Scale-Up of Phytosterols Bioconversion into Androstenedione Sonia Martнnez-Cбmara, Esther Bahнllo, Josй-Luis Barredo, and Marta Rodrнguez-Sбiz 15. Bioconversion of Phytosterols into Androstadienedione by Mycobacterium smegmatis CECT 8331 Julia Garcнa-Fernбndez, Igor Martнnez, Lorena Fernбndez-Cabezуn, Carmen Felpeto-Santero, Josй-Luis Garcнa, and Beatriz Galбn 16. Process for Biotransformation of Androsta-4-ene-3, 17-Dione (4-AD) to Androsta-1, 4-Diene-3, 17-Dione (ADD) Surya Prakash and Abhay Bajaj 17. Laboratory-Scale Hydroxylation of Steroids by P450BM3 Variants Sabrina Hoebenreich, Martin Spinck, and Nathalie Nett 18. Obtaining of 11α-Hydroxy and Rost-4-ene-3, 17-Dione from Natural Sterols Dmitry Dovbnya, Sergey Khomutov, Vyacheslav Kollerov, Marina Donova 19. Steroid 11-Alfa-Hydroxylation by the Fungi Aspergillus nidulans and Aspergillus ochraceus Lidia Ortega-de los Rнos, Josй M. Luengo, and Josй M. Fernбndez-Caсуn 20. Biotransformation of DHEA into 7α, 15α-diOH-DHEA Hui Li, Jin Sun, and Zhenghong Xu 21. Stabilization of Enzymes by Using Thermophiles Ana-Luisa Ribeiro, Mercedes Sбnchez, Aurelio Hidalgo, and Josй Berenguer
22. Biocatalysis of Steroids with Mycobacterium Sp. in Aqueous and Organic Media Carla C. C. R. de Carvalho and Pedro Fernandes
23. Downstream Process Synthesis for Microbial Steroids Fabian B. Thygs and Juliane Merz 24. Analysis of Intermediates of Steroid Transformations in Resting Cells by Thin Layer Chromatography (TLC) Govinda Guevara, Juliбn Perera, and Juana-Marнa Navarro-Llorens
Автор: Kelly M. Fulton (Editor), Susan M. Twine (Editor) Название: Immunoproteomics: Methods and Protocols (Methods in Molecular Biology) ISBN: 1627035885 ISBN-13(EAN): 9781627035880 Издательство: Springer Рейтинг: Цена: 23757.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Providing diverse, up-to-date approaches and techniques for studying the immunoproteome, this Methods in Molecular Biology book offers materials lists, step-by-step reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
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