Mucins: Methods and Protocols, McGuckin Michael A., Thornton David J.
Автор: Bailon Pascal Название: Affinity Chromatography / Methods and Protocols ISBN: 0896036944 ISBN-13(EAN): 9780896036949 Издательство: Springer Цена: 7540.00 р. Наличие на складе: Поставка под заказ.
Автор: Peter Molnar; James J. Hickman Название: Patch-Clamp Methods and Protocols ISBN: 1617377244 ISBN-13(EAN): 9781617377242 Издательство: Springer Рейтинг: Цена: 21661.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Patch Clamp Methods and Protocols surveys the typical patch clamp applications and advises scientists on identifying problems and selecting the best technique in each instance. The experiments described aid the researcher in pursuing new areas of electrophysiology and using the patch clamp technique effectively.
Автор: Moulton Hong M., Moulton Jon D. Название: Morpholino Oligomers: Methods and Protocols ISBN: 1493983075 ISBN-13(EAN): 9781493983070 Издательство: Springer Рейтинг: Цена: 19564.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
Invention and Early History of Morpholinos: From Pipe Dream to Practical Products.- Making a Morpholino Experiment Work: Controls, Favoring Specificity, Improving Efficacy, Storage, and Dose.- Aggregation and Disaggregation of Morpholino Oligomers in Solution.- End-Modifications on Morpholino Oligos.- Inducible Inhibition of Gene Function with Photomorpholinos.- Blocking Zebrafish microRNAs with Morpholinos.- Using Morpholinos to Examine Gene Function During Fin Regeneration.- Using Morpholinos to Probe Gene Networks in Sea Urchin.- Manipulating Gene Expression in the Chick Embryo.- Inhibition of Bacterial Growth by Peptide-conjugated Morpholino Oligomers.- Use of Translation Blocking Morpholinos for Gene Knock-Down in Giardia lamblia.- Regulation of Isoform Expression by Blocking Polyadenylation Signal Sequences with Morpholinos.- Targeting Functional Non-coding RNAs.- Use of Morpholino Oligomers for Pretargeting.- Diagnostic Applications of Morpholinos and Label-free Electrochemical Detection of Nucleic Acids.- Intranasal Delivery of Peptide-Morpholinos to Knockdown Influenza Host Factors in Mice.- Systemic Delivery of Morpholinos to Skip Multiple Exons in a Dog Model of Duchenne Muscular Dystrophy.- In vivo and Explant Electroporation of Morpholinos in the Developing Mouse Retina.
Автор: Barredo Josй-Luis, Herrбiz Ignacio Название: Microbial Steroids: Methods and Protocols ISBN: 1493984101 ISBN-13(EAN): 9781493984107 Издательство: Springer Рейтинг: Цена: 16769.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
Preface... Table of Contents... Contributing Authors...
1. Steroid Bioconversions Marina V. Donova 2. Chemical Pathways of Corticosteroids, Industrial Synthesis from Sapogenins Ignacio Herrбiz 3. Genome Sequencing of Steroid Producing Bacteria with Illumina Technology Victoria Y. Shtratnikova, Mikhail I. Schelkunov, and marina V. Donova 4. Genome Sequencing of Steroid Producing Bacteria using Ion Torrent Technology and a Reference Genome Alberto Sola-Landa, Antonio Rodrнguez-Garcнa, Carlos Barreiro, and Rosario Pйrez-Redondo 5. RNA-Seq-Based Comparative Transcriptomics: RNA Preparation and Bioinformatics Antonio Rodrнguez-Garcнa, Alberto Sola-Landa, and Carlos Barreiro 6. Intra- and Extra-Cellular Proteome Analyses of Steroid-Producer Mycobacteria Carlos Barreiro, Alejandro Morales, Inйs Vбzquez-Iglesias, and Alberto Sola-Landa 7. Genetic Techniques for Manipulation of the Phytosterol Biotransformation Strain Mycobacteriumneoaurum NRRL B-3805 Jessica K. Loraine and Margaret C. M. Smith 8. Identification and Characterization of the genes and Enzymes Belonging to the Bile Acid Catabolic Pathway in Pseudomonas Josй M. Luengo and Elias R. Olivera 9. Selection of Biodegrading Phytosterol Strains Marнa-Angйlica Mondaca, Maricel Vidal, Soledad Chamorro, and Gladys Vidal 10. Stigmasterol Removal by an Aerobic Treatment System Soledad Chamorro, Claudia Xavier, Vнctor Hernбndez, Josй Becerra, and Gladys Vidal 11. Production and Biotransformation of Phytosterol Microdispersions to Produce 4-Androstene-3, 17-Dione Rodrigo A. Mancilla, Roberto Pavez-Dнaz, and Andrea Muсoz-Orrego 12. Β-Sitosterol Bioconversion to Androstenedione in Microtiter Plates Marco P.C. Marques and Pedro Fernandez 13. Bioconversion of Phytosterols into Androstenedione by Mycobacterium Kjell D. Josefsen, Anna Nordborg, and Hеvard Sletta 14. Scale-Up of Phytosterols Bioconversion into Androstenedione Sonia Martнnez-Cбmara, Esther Bahнllo, Josй-Luis Barredo, and Marta Rodrнguez-Sбiz 15. Bioconversion of Phytosterols into Androstadienedione by Mycobacterium smegmatis CECT 8331 Julia Garcнa-Fernбndez, Igor Martнnez, Lorena Fernбndez-Cabezуn, Carmen Felpeto-Santero, Josй-Luis Garcнa, and Beatriz Galбn 16. Process for Biotransformation of Androsta-4-ene-3, 17-Dione (4-AD) to Androsta-1, 4-Diene-3, 17-Dione (ADD) Surya Prakash and Abhay Bajaj 17. Laboratory-Scale Hydroxylation of Steroids by P450BM3 Variants Sabrina Hoebenreich, Martin Spinck, and Nathalie Nett 18. Obtaining of 11α-Hydroxy and Rost-4-ene-3, 17-Dione from Natural Sterols Dmitry Dovbnya, Sergey Khomutov, Vyacheslav Kollerov, Marina Donova 19. Steroid 11-Alfa-Hydroxylation by the Fungi Aspergillus nidulans and Aspergillus ochraceus Lidia Ortega-de los Rнos, Josй M. Luengo, and Josй M. Fernбndez-Caсуn 20. Biotransformation of DHEA into 7α, 15α-diOH-DHEA Hui Li, Jin Sun, and Zhenghong Xu 21. Stabilization of Enzymes by Using Thermophiles Ana-Luisa Ribeiro, Mercedes Sбnchez, Aurelio Hidalgo, and Josй Berenguer
22. Biocatalysis of Steroids with Mycobacterium Sp. in Aqueous and Organic Media Carla C. C. R. de Carvalho and Pedro Fernandes
23. Downstream Process Synthesis for Microbial Steroids Fabian B. Thygs and Juliane Merz 24. Analysis of Intermediates of Steroid Transformations in Resting Cells by Thin Layer Chromatography (TLC) Govinda Guevara, Juliбn Perera, and Juana-Marнa Navarro-Llorens
Автор: Anthony P. Corfield Название: Glycoprotein Methods and Protocols ISBN: 1617371491 ISBN-13(EAN): 9781617371493 Издательство: Springer Рейтинг: Цена: 26552.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: The mucins (mucus glycoproteins) have long been a complex corner of glycoprotein biology. Isolation techniques that protect the intact mucins and allow dissociation from other adsorbed but discrete molecules needed to be developed and accepted by all researchers in the field.
Автор: Kragelund Birthe B., Skriver Karen Название: Intrinsically Disordered Proteins: Methods and Protocols ISBN: 1071605232 ISBN-13(EAN): 9781071605233 Издательство: Springer Рейтинг: Цена: 39130.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: The edition details methods to study intrinsically disordered proteins (IDPs) including recent topics such as extremely high-affinity disordered complexes, kinetics that evade established concepts, liquid-liquid phase separation, and novel disorder-driven allosteric mechanisms. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Intrinsically Disordered Proteins: Methods and Protocols aims to help scientists with different backgrounds to further their investigations into these fascinating and dynamic molecules. Chapter 24 is available open access under a CC BY 4.0 license via link.springer.com. Chapters “40 and 42 ” are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Автор: Spampinato Santi M. Название: Opioid Receptors: Methods and Protocols ISBN: 1071608835 ISBN-13(EAN): 9781071608838 Издательство: Springer Цена: 18167.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Chapters detail transcriptional and post-transcriptional analysis, cellular detection of opioid receptors, analysis of signaling events modulated by opioid receptors, model systems to studying opioid receptor-mediated functions, and behavioral effects mediated by opioid receptors.
Описание: In this present volume, different approaches are detailed to produce membrane proteins, purify them, study their function, determine their structure, and model them in membrane.
Автор: Vila-Perellу Miquel Название: Expressed Protein Ligation: Methods and Protocols ISBN: 1071604333 ISBN-13(EAN): 9781071604335 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Поставка под заказ.
Описание: This book provides a comprehensive overview of Expressed Protein Ligation (EPL), detailing methods and protocols to generate site-specifically modified proteins. Chapters include an overview of the protein semi-synthesis field, as well as related areas that have contributed to the development of EPL such as protein splicing and peptide synthesis. Following the introductory chapters, the rest of the book guides readers through protocols to perform EPL reactions, methods to synthesize peptide thioesters and to perform peptide and protein ligations, label proteins inside living cells, protocols for the semi-synthesis of phorphorylated, glycosylated and ubiquitylated proteins, synthesis and assembly of assymetrically modified nucleosomes, use of ligation auxiliaries and synthesis of cyclic proteins, as well as novel desulfurization strategies and use of selective Cys side chain protection to obtain precisely modified proteins.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Expressed Protein Ligation: Methods and Protocols will ensure successful implementation of protein semi-synthesis methods to further study the structure and function of proteins.
Автор: Kihara Daisuke Название: Protein Function Prediction: Methods and Protocols ISBN: 1493983687 ISBN-13(EAN): 9781493983681 Издательство: Springer Рейтинг: Цена: 19564.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Preface... Table of Contents... Contributing Authors... 1. Using PFP and ESG Protein Function Prediction Web Servers Qing Wei, Joshua McGraw, Ishita Khan, and Daisuke Kihara 2. GHOSTX: A Fast Sequence Homology Search Tool for Functional Annotation of Metagenomic Data Shuji Suzuki, Takashi Ishida, Masahito Ohue, Masanori Kakuta, and Yutaka Akiyama 3. From Gene Annotation to Function Prediction for Metagenomics Fatemeh Sharifi and Yuzhen Ye 4. An Agile Functional Analysis of Metagenomic Data using SUPER-FOCUS Genivaldo Gueiros Z. Silva, Fabyano A. C. Lopes, and Robert A. Edwards 5. MPFit: Computational Tool for Predicting Moonlighting Proteins Ishita Khan, Joshua McGraw, and Daisuke Kihara 6. Predicting Secretory Proteins with SignalP Henrik Nielsen 7. The ProFunc Function Prediction Server Roman A. Laskowski 8. G-LoSA for Prediction of Protein-Ligand Binding Sites and Structures Hui Sun Lee andWonpil Im 9. Local Alignment of Ligand Binding Sites in Proteins for Polypharmacology and Drug Repositioning Michal Brylinski 10. WATsite2.0 with PyMOL Plugin: Hydration Site Prediction and Visualization Ying Yang, Bingjie Hu, and Markus A. Lill 11. Enzyme Annotation and Metabolic Reconstruction Using KEGG Minoru Kanehisa 12. Ortholog Identification and Comparative Analysis of Microbial Genomes using MBGD and RECOG Ikuo Uchiyama 13. Exploring Protein Function Using the Saccharomyces Genome Database Edith D. Wong 14. Network-Based Gene Function Prediction in Mouse and Other Model Vertebrates using MouseNet Server Eiru Kim and Insuk Lee 15. The FANTOM5 Computation Ecosystem: Genomic Information Hub for Promoters and Active Enhancers Imad Abugessaisa, Shuhei Noguchi, Piero Carninci, and Takeya Kasukawa 16. Multi-Algorithm Particle Simulations with Spatiocyte Satya N. V. Arjunan and Koichi Takahashi
Автор: Greening David W., Simpson Richard J. Название: Serum/Plasma Proteomics: Methods and Protocols ISBN: 1493983806 ISBN-13(EAN): 9781493983803 Издательство: Springer Рейтинг: Цена: 16769.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
Part I Updated Blood Processing and Handling Strategies
1. Direct Assessment of Plasma/Serum Sample Quality for Proteomics Biomarker Investigation
Viviana Greco, Cristian Piras, Luisa Pieroni, and Andrea Urbani
2. A Protocol for the Preparation of Cryoprecipitate and Cryo-Depleted Plasma
Rosemary L Sparrow, David W Greening, and Richard J Simpson
3. A Proteomic Protocol for the Preparation of Platelet Concentrates
David W Greening, Richard J Simpson, and Rosemary L Sparrow
Part II Updated Fractionation Strategies for In-depth Blood Proteome Analysis
4. Bead-based and Multiplexed Immunoassays for Protein Profiling via Sequential Affinity Capture
Elin Birgersson, Jochen M. Schwenk, and Burcu Ayoglu
5. Affinity Proteomics for Fast, Sensitive, Quantitative Analysis of Proteins in Plasma
John P O'Grady, Kevin W Meyer, and Derrick N Poe
6. Characterization of the Low-molecular Weight Human Plasma Peptidome
David W. Greening and Richard J. Simpson
7. In-depth, Reproducible Analysis of Human Plasma using IgY 14 and SuperMix Immunodepletion
Lynn A. Beer, Bonnie Ky, Kurt T. Barnhart, and David W. Speicher
8. Low-Molecular-Weight Plasma Proteome Analysis Using Top-Down Mass Spectrometry
Dong Huey Cheon, Eun Gyeong Yang, Cheolju Lee, and Ji Eun Lee
9. Identification of Post-Translational Modifications from Serum/Plasma by Immunoaffinity Enrichment and LC-MS/MS Analysis without Depletion of Abundant Proteins
Hongbo Gu, Jianmin Ren, Xiaoying Jia, and Matthew P. Stokes
10. Identification of Core-fucosylated Glycoproteome in Human Plasma
Qichen Cao, Qing Zhao, Xiaohong Qian, and Wantao Ying
Part III Updated Proteome Analysis of Blood Cell Components, Vesicles, and Blood-related Fluids
11. Proteomic Analysis of Blood Extracellular Vesicles in Cardiovascular Disease by LC-MS/MS Analysis
Montserrat Baldan-Martin, Fernando de la Cuesta, Gloria Alvarez-Llamas, Gema Ruiz-Hurtado, Luis M. Ruilope, and Maria G. Barderas
12. Targeted Approach For Proteomic Analysis of a Hidden Membrane Protein
Tania Martins-Marques, Sandra I. Anjo, Teresa Ribeiro-Rodrigues, Bruno Manadas, and Henrique Girao
13. Red Blood Cells in Clinical Proteomics
Ana Sofia Carvalho, Manuel S Rodriguez, and Rune Matthiesen
14. High-throughput Quantitative Lipidomics Analysis of Non-esterified Fatty Acids in Plasma by LC-MS
Nicolas Christinat, Delphine Morin-Rivron, and Mojgan Masoodi
15. Simultaneous Enrichment of Plasma Extracellular Vesicles and Glycoproteome for Studying Disease Biomarkers
Sunil S. Adav and Siu Kwan Sze
16. Lipidomics of Human Blood Plasma by High Resolution Shotgun Mass Spectrometry
Susanne Sales, Oskar Knittelfelder, and Andrej Shevchenko
17. Proteomics Analysis of Circulating Serum Exosomes
Antonius Koller, Purvi Patel, Jenny Kim Kim, and Emily I. Chen
Part IV Antibody-based Discovery and Targeted Proteomics
18. High-density Serum/Plasma Reverse Phase Protein Arrays
Cecilia Hellstrцm, Tea Dodig-Crnkovic, Mun-Gwan Hong, Jochen M. Schwenk, Peter Nilsson, and Ronald Sjцberg
19. Antibody Colocalization Microarray for Cross-reactivity free Multiplexed Protein Analysis
Vйronique Laforte, Pik-Shan Lo, Huiyan Li, and David Juncker
20. Surface Profiling of Extracellular Vesicles From Plasma or Ascites Fluid using Dotscan Antibody Microarrays
Larissa Belov, Susannah Hallal, Kieran Matic, Jerry Zhou, Sandra Wissmueller, Nuzhat Ahmed, Sumaiya Tanjil, Stephen P. Mulligan, O. Giles Best, Richard J. Simpson, and Richard I. Christopherson
Автор: Kelly M. Fulton (Editor), Susan M. Twine (Editor) Название: Immunoproteomics: Methods and Protocols (Methods in Molecular Biology) ISBN: 1627035885 ISBN-13(EAN): 9781627035880 Издательство: Springer Рейтинг: Цена: 23757.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Providing diverse, up-to-date approaches and techniques for studying the immunoproteome, this Methods in Molecular Biology book offers materials lists, step-by-step reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
ООО "Логосфера " Тел:+7(495) 980-12-10 www.logobook.ru
