Serum/Plasma Proteomics: Methods and Protocols, Greening David W., Simpson Richard J.
Автор: Gary B. Smejkal and Alexander Lazarev Название: Separation Methods In Proteomics ISBN: 0824726995 ISBN-13(EAN): 9780824726997 Издательство: Taylor&Francis Рейтинг: Цена: 33686.00 р. Наличие на складе: Поставка под заказ.
Описание: Driven by the widespread growth of proteomic practices, protein separation techniques have been refined to minimize variability, optimize particular
applications, and adapt to user preferences in the analysis of proteins. "Separation Methods in Proteomics" provides a comprehensive examination of all major separation techniques for
proteomics research. Written as a compilation of hands-on methods exemplified by the work of several recognized leaders in the field, this book may serve as a guide for selection of the
optimal separation strategies to solve particular biological problems.
Recent progress in the development of robust analytical techniques and instrumentation has created the
need for good quality biological samples that are subject to analysis.Emphasizing the importance of sample preparation, the book explains how proteomes can be divided into smaller,
less complicated 'subproteomes' for individual analysis. It also highlights several hybrid approaches that take into account protein interactions. Including applications of the separation
methods currently employed in proteomic analyses for both clinical and basic research, "Separation Methods in Proteomics" contains practical information that can enhance the current
and future endeavors of scientists in proteomics, genomics, transcriptomics, biomarker discovery, and drug discovery.
Автор: Bhupendra Pushkar Название: Protein Synthesis: Methods and Protocols ISBN: 1774077035 ISBN-13(EAN): 9781774077030 Издательство: Mare Nostrum (Eurospan) Рейтинг: Цена: 22730.00 р. Наличие на складе: Нет в наличии.
Описание: Takes readers through the various ways in which proteins are synthesized and then further used for other applications. The book throws light on chemical reactions taking place in the process, and lays down different kinds of methods and the procedures that are followed in protein synthesis.
Автор: David W. Greening; Richard J. Simpson Название: Serum/Plasma Proteomics ISBN: 1493970569 ISBN-13(EAN): 9781493970568 Издательство: Springer Рейтинг: Цена: 22359.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
Part I Updated Blood Processing and Handling Strategies
1. Direct Assessment of Plasma/Serum Sample Quality for Proteomics Biomarker Investigation
Viviana Greco, Cristian Piras, Luisa Pieroni, and Andrea Urbani
2. A Protocol for the Preparation of Cryoprecipitate and Cryo-Depleted Plasma
Rosemary L Sparrow, David W Greening, and Richard J Simpson
3. A Proteomic Protocol for the Preparation of Platelet Concentrates
David W Greening, Richard J Simpson, and Rosemary L Sparrow
Part II Updated Fractionation Strategies for In-depth Blood Proteome Analysis
4. Bead-based and Multiplexed Immunoassays for Protein Profiling via Sequential Affinity Capture
Elin Birgersson, Jochen M. Schwenk, and Burcu Ayoglu
5. Affinity Proteomics for Fast, Sensitive, Quantitative Analysis of Proteins in Plasma
John P O'Grady, Kevin W Meyer, and Derrick N Poe
6. Characterization of the Low-molecular Weight Human Plasma Peptidome
David W. Greening and Richard J. Simpson
7. In-depth, Reproducible Analysis of Human Plasma using IgY 14 and SuperMix Immunodepletion
Lynn A. Beer, Bonnie Ky, Kurt T. Barnhart, and David W. Speicher
8. Low-Molecular-Weight Plasma Proteome Analysis Using Top-Down Mass Spectrometry
Dong Huey Cheon, Eun Gyeong Yang, Cheolju Lee, and Ji Eun Lee
9. Identification of Post-Translational Modifications from Serum/Plasma by Immunoaffinity Enrichment and LC-MS/MS Analysis without Depletion of Abundant Proteins
Hongbo Gu, Jianmin Ren, Xiaoying Jia, and Matthew P. Stokes
10. Identification of Core-fucosylated Glycoproteome in Human Plasma
Qichen Cao, Qing Zhao, Xiaohong Qian, and Wantao Ying
Part III Updated Proteome Analysis of Blood Cell Components, Vesicles, and Blood-related Fluids
11. Proteomic Analysis of Blood Extracellular Vesicles in Cardiovascular Disease by LC-MS/MS Analysis
Montserrat Baldan-Martin, Fernando de la Cuesta, Gloria Alvarez-Llamas, Gema Ruiz-Hurtado, Luis M. Ruilope, and Maria G. Barderas
12. Targeted Approach For Proteomic Analysis of a Hidden Membrane Protein
Tania Martins-Marques, Sandra I. Anjo, Teresa Ribeiro-Rodrigues, Bruno Manadas, and Henrique Girao
13. Red Blood Cells in Clinical Proteomics
Ana Sofia Carvalho, Manuel S Rodriguez, and Rune Matthiesen
14. High-throughput Quantitative Lipidomics Analysis of Non-esterified Fatty Acids in Plasma by LC-MS
Nicolas Christinat, Delphine Morin-Rivron, and Mojgan Masoodi
15. Simultaneous Enrichment of Plasma Extracellular Vesicles and Glycoproteome for Studying Disease Biomarkers
Sunil S. Adav and Siu Kwan Sze
16. Lipidomics of Human Blood Plasma by High Resolution Shotgun Mass Spectrometry
Susanne Sales, Oskar Knittelfelder, and Andrej Shevchenko
17. Proteomics Analysis of Circulating Serum Exosomes
Antonius Koller, Purvi Patel, Jenny Kim Kim, and Emily I. Chen
Part IV Antibody-based Discovery and Targeted Proteomics
18. High-density Serum/Plasma Reverse Phase Protein Arrays
Cecilia Hellstrцm, Tea Dodig-Crnkovic, Mun-Gwan Hong, Jochen M. Schwenk, Peter Nilsson, and Ronald Sjцberg
19. Antibody Colocalization Microarray for Cross-reactivity free Multiplexed Protein Analysis
Vйronique Laforte, Pik-Shan Lo, Huiyan Li, and David Juncker
20. Surface Profiling of Extracellular Vesicles From Plasma or Ascites Fluid using Dotscan Antibody Microarrays
Larissa Belov, Susannah Hallal, Kieran Matic, Jerry Zhou, Sandra Wissmueller, Nuzhat Ahmed, Sumaiya Tanjil, Stephen P. Mulligan, O. Giles Best, Richard J. Simpson, and Richard I. Christopherson
Автор: Martin A. Masuelli Название: Bovine Serum Albumin: Properties and Applications ISBN: 1536167878 ISBN-13(EAN): 9781536167870 Издательство: Nova Science Рейтинг: Цена: 22491.00 р. Наличие на складе: Невозможна поставка.
Описание: Serum albumin is a large protein present in cow serum protein (BSA, Bovine Serum Albumin) rich in essential amino acids and compounds with disulfide bridges and thiol groups. This protein passes into breast milk from the bloodstream by diffusion, has antioxidant capacity and protects fats from oxidation. Albumin has the ability to inhibit tumor factors and bind to fat, mobilize them in the body to perform other functions such as obtaining energy or forming membranes. It is also able to block the conversion of angiotensin I into angiotensin II (vasoconstrictor) which gives it antihypertensive action. In addition, it has the ability to reduce cholesterol absorption, complex heavy metals and is an opioid agonist (acts on opioid receptors and promotes analgesia). BSA is the most studied and produced protein worldwide in the last 80 years. This protein brings large dividends to the world economy with a very prominent and favorable annual growth at a cost of 100 USD per gram. This book brings new contributions to the science and industry of this biopolymer. The audience of this book is very wide from students, teachers and researchers (doctors, pharmacists, biochemists and industrialists of this sector). Therefore, this book has a great future and many readers to whom it is addressed.
Название: Separation Methods In Proteomics ISBN: 0367391570 ISBN-13(EAN): 9780367391577 Издательство: Taylor&Francis Рейтинг: Цена: 9798.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
Driven by the widespread growth of proteomic practices, protein separation techniques have been refined to minimize variability, optimize particular applications, and adapt to user preferences in the analysis of proteins.
Separation Methods in Proteomics provides a comprehensive examination of all major separation techniques for proteomics research.
Written as a compilation of hands-on methods exemplified by the work of several recognized leaders in the field, this book may serve as a guide for selection of the optimal separation strategies to solve particular biological problems.
Recent progress in the development of robust analytical techniques and instrumentation has created the need for good quality biological samples that are subject to analysis. Emphasizing the importance of sample preparation, the book explains how proteomes can be divided into smaller, less complicated "subproteomes for individual analysis. It also highlights several hybrid approaches that take into account protein interactions.
Including applications of the separation methods currently employed in proteomic analyses for both clinical and basic research, Separation Methods in Proteomics contains practical information that can enhance the current and future endeavors of scientists in proteomics, genomics, transcriptomics, biomarker discovery, and drug discovery.
Автор: Matthiesen Rune Название: Proteostasis: Methods and Protocols ISBN: 1493981307 ISBN-13(EAN): 9781493981304 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume highlights the role of proteostasis in human health and associated disease model systems, reflecting its rising importance which has led to the development of new technologies to obtain insight into underling protein mechanistic events. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Proteostasis: Methods and Protocols aims to become a reference book on proteostasis in human health.
Автор: McGuckin Michael A., Thornton David J. Название: Mucins: Methods and Protocols ISBN: 1493958860 ISBN-13(EAN): 9781493958863 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Epithelial mucins are large complex cell surface and secreted glycoproteins produced by mucosal epithelial cells. In, Mucins: Methods and Protocols expert researchers in the field detail many of the methods which are now commonly used to study Mucins. These include methods and techniques for the best approaches to analysing each specific area of mucin biochemistry, physiology and biophysics before providing individual detailed experimental protocols together with troubleshooting and interpretation tips. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Mucins: Methods and Protocols is designed to be a useful resource for those entering the mucin field and to facilitate those already studying mucins to broaden their experimental approaches to understanding mucosal biology.
Автор: Vila-Perellу Miquel Название: Expressed Protein Ligation: Methods and Protocols ISBN: 1071604333 ISBN-13(EAN): 9781071604335 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Поставка под заказ.
Описание: This book provides a comprehensive overview of Expressed Protein Ligation (EPL), detailing methods and protocols to generate site-specifically modified proteins. Chapters include an overview of the protein semi-synthesis field, as well as related areas that have contributed to the development of EPL such as protein splicing and peptide synthesis. Following the introductory chapters, the rest of the book guides readers through protocols to perform EPL reactions, methods to synthesize peptide thioesters and to perform peptide and protein ligations, label proteins inside living cells, protocols for the semi-synthesis of phorphorylated, glycosylated and ubiquitylated proteins, synthesis and assembly of assymetrically modified nucleosomes, use of ligation auxiliaries and synthesis of cyclic proteins, as well as novel desulfurization strategies and use of selective Cys side chain protection to obtain precisely modified proteins.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Expressed Protein Ligation: Methods and Protocols will ensure successful implementation of protein semi-synthesis methods to further study the structure and function of proteins.
Описание: In this present volume, different approaches are detailed to produce membrane proteins, purify them, study their function, determine their structure, and model them in membrane.
Автор: Hobman Tom C., Duchaine Thomas F. Название: Argonaute Proteins: Methods and Protocols ISBN: 1493958275 ISBN-13(EAN): 9781493958276 Издательство: Springer Рейтинг: Цена: 20263.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: With the rapid proliferation of RNAi applications in basic and clinical sciences, the challenge has now become understanding how components of RNAi machinery function together in a regulated manner. Argonaute proteins are the central effectors of RNAi and are highly conserved among eukaryotes and some archaebacteria. These RNA-binding proteins use small guide RNAs to silence expression of genes at the mRNA and DNA levels. In Argonaute Proteins: Methods and Protocols, expert researchers in this burgeoning field provide detailed, up-to-date methods to study Argonaute protein functions and interactions in a wide variety of cell types ranging from yeast to mammalian systems, as well as in vitro. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, Argonaute Proteins: Methods and Protocols serves as a vital reference for both experienced and novice scientists approaching the vast complexities of RNAi research.
Автор: Kihara Daisuke Название: Protein Function Prediction: Methods and Protocols ISBN: 1493983687 ISBN-13(EAN): 9781493983681 Издательство: Springer Рейтинг: Цена: 19564.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Preface... Table of Contents... Contributing Authors... 1. Using PFP and ESG Protein Function Prediction Web Servers Qing Wei, Joshua McGraw, Ishita Khan, and Daisuke Kihara 2. GHOSTX: A Fast Sequence Homology Search Tool for Functional Annotation of Metagenomic Data Shuji Suzuki, Takashi Ishida, Masahito Ohue, Masanori Kakuta, and Yutaka Akiyama 3. From Gene Annotation to Function Prediction for Metagenomics Fatemeh Sharifi and Yuzhen Ye 4. An Agile Functional Analysis of Metagenomic Data using SUPER-FOCUS Genivaldo Gueiros Z. Silva, Fabyano A. C. Lopes, and Robert A. Edwards 5. MPFit: Computational Tool for Predicting Moonlighting Proteins Ishita Khan, Joshua McGraw, and Daisuke Kihara 6. Predicting Secretory Proteins with SignalP Henrik Nielsen 7. The ProFunc Function Prediction Server Roman A. Laskowski 8. G-LoSA for Prediction of Protein-Ligand Binding Sites and Structures Hui Sun Lee andWonpil Im 9. Local Alignment of Ligand Binding Sites in Proteins for Polypharmacology and Drug Repositioning Michal Brylinski 10. WATsite2.0 with PyMOL Plugin: Hydration Site Prediction and Visualization Ying Yang, Bingjie Hu, and Markus A. Lill 11. Enzyme Annotation and Metabolic Reconstruction Using KEGG Minoru Kanehisa 12. Ortholog Identification and Comparative Analysis of Microbial Genomes using MBGD and RECOG Ikuo Uchiyama 13. Exploring Protein Function Using the Saccharomyces Genome Database Edith D. Wong 14. Network-Based Gene Function Prediction in Mouse and Other Model Vertebrates using MouseNet Server Eiru Kim and Insuk Lee 15. The FANTOM5 Computation Ecosystem: Genomic Information Hub for Promoters and Active Enhancers Imad Abugessaisa, Shuhei Noguchi, Piero Carninci, and Takeya Kasukawa 16. Multi-Algorithm Particle Simulations with Spatiocyte Satya N. V. Arjunan and Koichi Takahashi
Автор: Comai Lucio, Katz Jonathan E., Mallick Parag Название: Proteomics: Methods and Protocols ISBN: 1493982907 ISBN-13(EAN): 9781493982905 Издательство: Springer Рейтинг: Цена: 20962.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume aims to provide protocols on a wide range of biochemical methods, analytical approaches, and bioinformatics tools developed to analyze the proteome.
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