Argonaute Proteins: Methods and Protocols, Hobman Tom C., Duchaine Thomas F.

Автор: Bhupendra Pushkar
Название: Protein Synthesis: Methods and Protocols
ISBN: 1774077035 ISBN-13(EAN): 9781774077030
Издательство: Mare Nostrum (Eurospan)
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Описание: Takes readers through the various ways in which proteins are synthesized and then further used for other applications. The book throws light on chemical reactions taking place in the process, and lays down different kinds of methods and the procedures that are followed in protein synthesis.

Автор: Schueler-Furman, Ora;London, Nir
Название: Modeling Peptide-Protein Interactions : Methods and Protocols
ISBN: 1493967967 ISBN-13(EAN): 9781493967964
Издательство: Springer
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Цена: 13974.00 р.
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Описание: This volume covers an array of techniques available for studying peptide-protein docking and design.

Автор: Katsutomo Okamura; Kotaro Nakanishi
Название: Argonaute Proteins
ISBN: 1493984578 ISBN-13(EAN): 9781493984572
Издательство: Springer
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Описание: This volume covers methods that analyze various Argonaute proteins from a variety of organisms to help researchers better understand their properties ranging from a molecular level to an organismal level. The chapters in this book explore the following topics: identification and expression analysis of guide nucleic acids and their targets; analysis of biochemical properties of Argonautes; biological functions of Argonautes; and obtaining materials and setting up analysis platforms. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Argonaute Proteins: Methods and Protocols is a valuable resource for researchers and scientists looking to expand their knowledge of Argonaute proteins and their functions.

Автор: Katsutomo Okamura; Kotaro Nakanishi
Название: Argonaute Proteins
ISBN: 149397338X ISBN-13(EAN): 9781493973385
Издательство: Springer
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Описание: This volume covers methods that analyze various Argonaute proteins from a variety of organisms to help researchers better understand their properties ranging from a molecular level to an organismal level.

Автор: Lanzuolo Chiara, Bodega Beatrice
Название: Polycomb Group Proteins: Methods and Protocols
ISBN: 1493981811 ISBN-13(EAN): 9781493981816
Издательство: Springer
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Цена: 15372.00 р.
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Описание:

Mapping the Function of Polycomb Proteins.- Chromatin Immunoprecipitation.- Chromatin preparation and Chromatin Immuno-Precipitation from Drosophila embryos.- ChIP-seq data processing for PcG proteins and associated histone modifications.- Analysis of single locus replication timing in asynchronous cycling cells.- Non coding RNA interplay with the genome.- RIP: RNA Immunoprecipitation.- Capture Hybridization Analysis of DNA Targets.- Identification of RNA-protein interactions through in-vitro RNA pull-down assays.- Understanding RNA-chromatin interactions using Chromatin Isolation by RNA Purification (ChIRP).- Analysis RNA-seq and non coding RNA.- The dynamics of Polycomb complexes.- Fluorescence resonance energy transfer microscopy for measuring chromatin complex structure and dynamics.- Analysis of endogenous protein interactions of Polycomb-group of proteins in mouse embryonic stem cells.- Determination of Polycomb group of proteins compartmentalization through chromatin fractionation procedure.- An Automatic Segmentation Method Combining an Active Contour Model and a Classification Technique for Detecting Polycomb-group Proteins in High-Throughput Microscopy Images.- Polymer physics of the large-scale structure of chromatin.- Chromosome Conformation Capture in Drosophila.- Chromosome Conformation Capture in primary human cells.- Determination of high-resolution 3D chromatin organization using Circular Chromosome Conformation Capture (4C-seq).- Chromosome Conformation Capture on Chip (4C): Data Processing.- In vivo models to address the function of Polycomb Group Proteins.- A rapid TALEN assembly protocol.- Following the motion of Polycomb bodies in living Drosophila embryos.- Reprogramming of Somatic Cells towards Pluripotency by Cell Fusion.- Imaginal discs transplantation in Drosophila.- Isolation and culture of muscle stem cells.

Автор: Takigawa Masaharu
Название: Ccn Proteins: Methods and Protocols
ISBN: 1493981978 ISBN-13(EAN): 9781493981977
Издательство: Springer
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Цена: 17468.00 р.
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Описание: This volume provides a comprehensive guide on the Cyr61 (Cysteine-rich 61) (CCN) family of proteins and genes from basic research to cutting-edge methodologies and state-of-the-art techniques.

Автор: Vila-Perellу Miquel
Название: Expressed Protein Ligation: Methods and Protocols
ISBN: 1071604333 ISBN-13(EAN): 9781071604335
Издательство: Springer
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Цена: 15372.00 р.
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Описание: This book provides a comprehensive overview of Expressed Protein Ligation (EPL), detailing methods and protocols to generate site-specifically modified proteins. Chapters include an overview of the protein semi-synthesis field, as well as related areas that have contributed to the development of EPL such as protein splicing and peptide synthesis. Following the introductory chapters, the rest of the book guides readers through protocols to perform EPL reactions, methods to synthesize peptide thioesters and to perform peptide and protein ligations, label proteins inside living cells, protocols for the semi-synthesis of phorphorylated, glycosylated and ubiquitylated proteins, synthesis and assembly of assymetrically modified nucleosomes, use of ligation auxiliaries and synthesis of cyclic proteins, as well as novel desulfurization strategies and use of selective Cys side chain protection to obtain precisely modified proteins.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Expressed Protein Ligation: Methods and Protocols will ensure successful implementation of protein semi-synthesis methods to further study the structure and function of proteins.

Автор: Kihara Daisuke
Название: Protein Function Prediction: Methods and Protocols
ISBN: 1493983687 ISBN-13(EAN): 9781493983681
Издательство: Springer
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Описание: Preface...
Table of Contents...
Contributing Authors...

1. Using PFP and ESG Protein Function Prediction Web Servers
Qing Wei, Joshua McGraw, Ishita Khan, and Daisuke Kihara

2. GHOSTX: A Fast Sequence Homology Search Tool for Functional Annotation of Metagenomic Data
Shuji Suzuki, Takashi Ishida, Masahito Ohue, Masanori Kakuta, and Yutaka Akiyama

3. From Gene Annotation to Function Prediction for Metagenomics
Fatemeh Sharifi and Yuzhen Ye

4. An Agile Functional Analysis of Metagenomic Data using SUPER-FOCUS
Genivaldo Gueiros Z. Silva, Fabyano A. C. Lopes, and Robert A. Edwards

5. MPFit: Computational Tool for Predicting Moonlighting Proteins
Ishita Khan, Joshua McGraw, and Daisuke Kihara

6. Predicting Secretory Proteins with SignalP
Henrik Nielsen

7. The ProFunc Function Prediction Server
Roman A. Laskowski

8. G-LoSA for Prediction of Protein-Ligand Binding Sites and Structures
Hui Sun Lee and Wonpil Im

9. Local Alignment of Ligand Binding Sites in Proteins for Polypharmacology and Drug Repositioning
Michal Brylinski

10. WATsite2.0 with PyMOL Plugin: Hydration Site Prediction and Visualization
Ying Yang, Bingjie Hu, and Markus A. Lill

11. Enzyme Annotation and Metabolic Reconstruction Using KEGG
Minoru Kanehisa

12. Ortholog Identification and Comparative Analysis of Microbial Genomes using MBGD and RECOG
Ikuo Uchiyama

13. Exploring Protein Function Using the Saccharomyces Genome Database
Edith D. Wong

14. Network-Based Gene Function Prediction in Mouse and Other Model Vertebrates using MouseNet Server
Eiru Kim and Insuk Lee

15. The FANTOM5 Computation Ecosystem: Genomic Information Hub for Promoters and Active Enhancers
Imad Abugessaisa, Shuhei Noguchi, Piero Carninci, and Takeya Kasukawa

16. Multi-Algorithm Particle Simulations with Spatiocyte
Satya N. V. Arjunan and Koichi Takahashi

Автор: Lacapere Jean-Jacques
Название: Membrane Protein Structure and Function Characterization: Methods and Protocols
ISBN: 1493983997 ISBN-13(EAN): 9781493983995
Издательство: Springer
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Описание: In this present volume, different approaches are detailed to produce membrane proteins, purify them, study their function, determine their structure, and model them in membrane.

Автор: Abbott D. Wade, Lammerts Van Bueren Alicia
Название: Protein-Carbohydrate Interactions: Methods and Protocols
ISBN: 1493983326 ISBN-13(EAN): 9781493983322
Издательство: Springer
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Описание: This volume is a wide-ranging tool for studying protein-carbohydrate interactions that extend from traditional biochemical methods to state-of-the-art techniques.

Автор: Greening David W., Simpson Richard J.
Название: Serum/Plasma Proteomics: Methods and Protocols
ISBN: 1493983806 ISBN-13(EAN): 9781493983803
Издательство: Springer
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Цена: 16769.00 р.
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Описание:

Part I Updated Blood Processing and Handling Strategies

1. Direct Assessment of Plasma/Serum Sample Quality for Proteomics Biomarker Investigation

Viviana Greco, Cristian Piras, Luisa Pieroni, and Andrea Urbani

2. A Protocol for the Preparation of Cryoprecipitate and Cryo-Depleted Plasma

Rosemary L Sparrow, David W Greening, and Richard J Simpson

3. A Proteomic Protocol for the Preparation of Platelet Concentrates

David W Greening, Richard J Simpson, and Rosemary L Sparrow

Part II Updated Fractionation Strategies for In-depth Blood Proteome Analysis

4. Bead-based and Multiplexed Immunoassays for Protein Profiling via Sequential Affinity Capture

Elin Birgersson, Jochen M. Schwenk, and Burcu Ayoglu

Автор: Kister Alexander E.
Название: Protein Supersecondary Structures: Methods and Protocols
ISBN: 1493991604 ISBN-13(EAN): 9781493991600
Издательство: Springer
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Цена: 20962.00 р.
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Описание: