Single Molecule Analysis: Methods and Protocols, Peterman Erwin J. G., Wuite Gijs J. L.
Автор: Erwin J. G. Peterman Название: Single Molecule Analysis ISBN: 1493984373 ISBN-13(EAN): 9781493984374 Издательство: Springer Рейтинг: Цена: 20962.00 р. Наличие на складе: Поставка под заказ.
Описание: The aim of this volume is to provide a broad overview of single-molecule approaches applied to biomolecules. Chapters in Single Molecule Techniques: Methods and Protocols, Second Edition detail the most widely used single-molecule techniques, such as DNA, DNA-binding proteins, motor proteins, and are becoming commonplace in molecular biophysics, biochemistry, and molecular and cell biology. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Single Molecule Analysis: Methods and Protocols, Second Edition aims to be useful to many researchers, inspire them and help them to go single molecule.
Описание: Inthis thesis single-molecule fluorescence resonance energy transfer(FRET)spectroscopy was used to study the folding of a protein that belongsto the large and important family of repeat proteins.
Автор: Uttamchandani Mahesh, Yao Shao Q. Название: Small Molecule Microarrays: Methods and Protocols ISBN: 1493982427 ISBN-13(EAN): 9781493982424 Издательство: Springer Рейтинг: Цена: 13275.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
The Expanding World of Small Molecule Microarrays.- Novel Substrates for Microarrays.- Fabrication of Bio-function-preserved Saccharide Microarray Chips with Cyanuric Chloride as a Rotatable Linker.- Fabrication of Carbohydrate Microarrays by Boronate Formation.- Clickable Polymeric Coating for Glycan Microarrays.- A Versatile Microarray Immobilization Strategy Based on a Biorthogonal Reaction between Tetrazine and Trans-Cyclooctene.- Label-Free Sensing on Microarrays.- Optical Microscopy for Detecting Binding on Small Molecule Microarrays.- Array-on-array Strategy For Activity-based Enzyme Profiling.- Protein-Protein Interaction Inhibitors of BRCA1 Discovered Using Small Molecule Microarrays.- Discovery of RNA Binding Small Molecules Using Small Molecule Microarrays.- Profiling Phosphopeptide-binding Domain Recognition Specificity using Peptide Microarrays.- Validation Procedure for Multiplex Antibiotic Immunoassays Using Flow-based Chemiluminescence Microarrays.- Large-scale Interaction Profiling of Protein Domains through Proteomic Peptide-PhageDisplay using Custom Peptidomes.- Synthetic Glycan Microarrays.- Screening Mammalian Cells on a Hydrogel- Functionalized Small Molecule Microarray.
Автор: Erwin J. G. Peterman Название: Single Molecule Analysis ISBN: 1493972707 ISBN-13(EAN): 9781493972708 Издательство: Springer Рейтинг: Цена: 20962.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
Part I
1. Introduction to Optical Tweezers: Background, System Designs, and Commercial Solutions Joost van Mameren, Gijs J. L. Wuite, and Iddo Heller
2. RNA Unzipping and Force Measurements with a Dual Optical Trap Laurent Geffroy, Pierre Mangeol, Thierry Bizebard, and Ulrich Bockelmann
3. Protein Tethering for Folding Studies Fatemeh Moayed, Roeland J. van Wijk, David P. Minde, and Sander J. Tans
4. Combining Structure-Function and Single-Molecule Studies on Cytoplasmic Dynein Lu Rao, Maren Hьlsemann, and Arne Gennerich
5. A Brief Introduction to Single-Molecule Fluorescence Methods Siet M. J. L. van den Wildenberg, Bram Prevo, and Erwin J. G. Peterman
PART II
6. Fluorescent Labeling of Proteins Mauro Modesti
7. Single-Molecule Imaging of Escherichia coli Trans-Membrane Proteins Aravindan Varadarajan, Felix Oswald, and Yves J. M. Bollen
8. Single-Molecule Fluorescence Microscopy in Living Caenorhabditis elegans Jaap van Krugten and Erwin J. G. Peterman
9. Purification and Application of a Small Actin Probe for Single-Molecule Localization Microscopy Roderick P. Tas, Trusanne G. A. A. Bos, and Lukas C. Kapitein
10. Fluorescence Microscopy of Nanochannel-Confined DNA Fredrik Westerlund, Fredrik Persson, Joachim Fritsche, Jason P. Beech, and Jonas O. Tegenfeldt
11. Use of Single Molecule Fluorescence Polarization Microscopy to Study Protein Conformation and Dynamics of Kinesin--Microtubule Complexes Matthieu P. M. H. Benoit and Hernando Sosa
12. Single Molecule FRET Analysis of DNA Binding Proteins Kathy R. Chaurasiya and Remus T. Dame
PART III
13. Atomic Force Microscopy: An Introduction Melissa C. Piontek and Wouter H. Roos
14. Imaging of DNA and Protein by SFM and Combined SFM-TIRF Microscopy Malgorzata Grosbart, Dejan Ristic, Humberto Sбnchez, and Claire Wyman
15. Atomic Force Microscopy of Protein Shells: Virus Capsids and Beyond Alvaro Ortega-Esteban, N. Martнn-Gonzбlez, F. Moreno-Madrid, Aida Llaurу, Mercedes Hernando-Pйrez, and Pedro J. de Pablo
16. Combined Magnetic Tweezers and Micro-mirror Total Internal Reflection Fluorescence Microscope for Single Molecule Manipulation and Visualization Yeonee Seol and Keir C. Neuman
17. Tethered Particle Motion: An Easy Technique for Probing DNA Topology and Transcription Factor Interactions Daniel T. Tovari, Yan Yan, Laura Finzi, and David Dunlap
18. Single-Molecule Measurements Using Acoustic Force Spectroscopy (AFS) Douwe Kamsma, and Gijs J.L. Wuite
19. Repurposing a Benchtop Centrifuge for High-Throughput Single-Molecule Force Spectroscopy Daren Yang, and Wesley P. Wong
Автор: Bensimon, David (research Director And Professor, Lps-ens, Psl, Cnrs And Department Of Chemistry And Biochemistry, Ucla, Usa) Croquette, Vincent (re Название: Single molecule studies of nucleic acids and their associated proteins ISBN: 0198530927 ISBN-13(EAN): 9780198530923 Издательство: Oxford Academ Рейтинг: Цена: 11246.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This book is an introduction to the novel physical tools that allow single molecule manipulation and visualisation. It describes the use of these tools to understand the elastic properties of single molecules of DNA and RNA and to study their interactions with the many proteins that process them.
Описание: A Condensed History of Chromatin Research.- Investigating Chromatin Organisation Using Single Molecule Localisation Microscopy.- Structure, Function and Dynamics of Chromatin.- Periodic and Symmetric Organisation of Meiotic Chromosomes.- Conclusions.- Appendices.
Автор: Pellicciari Carlo, Biggiogera Marco Название: Histochemistry of Single Molecules: Methods and Protocols ISBN: 1493983016 ISBN-13(EAN): 9781493983018 Издательство: Springer Рейтинг: Цена: 20962.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: It begins with a section on vital histochemistry and continues with chapters on histochemistry as it relates to lectins, proteins, lipids, DNA and RNA, as well as plants.
Автор: Andres F. Oberhauser Название: Single-molecule Studies of Proteins ISBN: 1489991123 ISBN-13(EAN): 9781489991126 Издательство: Springer Рейтинг: Цена: 22201.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This book examines the successful application of single-molecule techniques to a wide range of biological events. It explores sophisticated advances in powerful new analytical, computational, and imaging methods.
Автор: Alexander E. Knight Название: Single Molecule Biology, ISBN: 0123742277 ISBN-13(EAN): 9780123742278 Издательство: Elsevier Science Рейтинг: Цена: 11789.00 р. Наличие на складе: Поставка под заказ.
Описание: Describes such single molecule techniques (that allow for manipulation and measurement of single biological molecules within a live cell) as single molecule fluorescence, optical tweezers, and scanning probe microscopy. This book is organized by biological system or molecule. It discusses applications in molecular diagnostics and drug discovery.
Автор: Gregory I. Mashanov; Christopher Batters Название: Single Molecule Enzymology ISBN: 149396190X ISBN-13(EAN): 9781493961900 Издательство: Springer Рейтинг: Цена: 20263.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This an ideal guide for the diverse community of molecular biologists, biochemists, and biophysicists studying enzymes in detail. Experts in the field provide procedures which enable the extraction of detailed information about enzyme work cycles.
Описание: Inthis thesis single-molecule fluorescence resonance energy transfer(FRET)spectroscopy was used to study the folding of a protein that belongsto the large and important family of repeat proteins.
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