Описание: As the first comprehensive overview of this important class of enzymes, this two-volume handbook summarizes recent knowledge about the molecular mechanism of ATPases, relating this information to the physiology and pathopyhsiology of ion transport, mitochondrial function, vesicle transport and lysosomal acidification. All important P-type, F-type and V-type ATPases are treated systematically, compmented by a special section on the cell biology and physiology of acidic compartments, and backed by an extensive bibliography and index. This premier reference source for physiologists, molecular biologists, biophysicists and clinical researchers contains contributions by more than 20 of the world's foremost ATPase research groups.
Автор: Mohammed Awad Ali Khlaid Название: Na+K+-ATPase: Discovery, Functions and Regulation ISBN: 1536189685 ISBN-13(EAN): 9781536189681 Издательство: Nova Science Рейтинг: Цена: 13462.00 р. Наличие на складе: Невозможна поставка.
Описание: Sodiumpotassium adenosine triphosphatase (Na+/K+-ATPase) is an enzyme located in the plasma membrane of most eukaryotic cells, responsible for maintaining ion concentration gradients against their concentration scale using ATP hydrolysis as a source of energy. The enzyme is involved in many cellular activities, including maintaining cell volume, transporting nutrition, electrical potential, neurotransmission, and any dysfunction or modification of enzyme activity is directly linked to many diseases, such as epilepsy, cardiovascular disease and hypertension, diabetes and other metabolic disorders, digoxin toxicity, fetal abnormalities, neurological disorders, and pulmonary conditions. In this book, the authors present current research on regulation strategies, Na⁺/K⁺-ATPase as a biomarker in diseased states, and the use of Na⁺/K⁺-ATPase in the physiological and ecological adaptations of insects. Other topics discussed in this compilation include the effects of hormonal and non-hormonal stimulation on Na+/K+-ATPase activity, the physiology and pathophysiology of Na+/K+-ATPase, Na⁺/K⁺-ATPase as a biomarker for energy metabolism and oxidative stress in diseased states, the neurotensin inhibitory effects of [3H]-Ouabain binding to striatal membranes and inversion by administration of clozapine, and Na+/K+-ATPases important functional roles in human body.
Автор: Maike Bublitz Название: P-Type ATPases ISBN: 1493979957 ISBN-13(EAN): 9781493979950 Издательство: Springer Рейтинг: Цена: 21661.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
1. An Introduction to P-type ATPase research
Poul Nissen
Part I: Protein Production, Isolation, Purification, and Stabilization
2. Purification of Na, K-ATPase from Pig Kidney
Natalya U. Fedosova
3. Preparation of Ca2+-ATPase1a Enzyme from Rabbit Sarcoplasmic Reticulum
Jesper V. M ller and Claus Olesen
4. Isolation of H+/K+-ATPase-enriched Membrane Fraction from Pig Stomachs
Kazuhiro Abe and Claus Olesen
5. Overproduction of PiB-type ATPases
Xiangyu Liu, Oleg Sitsel, Kaituo Wang, and Pontus Gourdon
6. Coordinated Overexpression in Yeast of a P4-ATPase and its Associated Cdc50 Subunit: The Case of the Drs2p/Cdc50p Lipid Flippase Complex
Hassina Azouaoui, Cйdric Montigny, Aurore Jacquot, Raphaлlle Barry, Philippe Champeil, and Guillaume Lenoir
7. The Plasma Membrane Ca2+-ATPase: Purification by Calmodulin Affinity Chromatography, and Reconstitution of the Purified Protein
Verena Niggli and Ernesto Carafoli
8. Expression of Na, K-ATPase and H, K-ATPase Isoforms With The Baculovirus Expression System
Jan B. Koenderink and Herman G.P. Swarts
9. Time-dependent Protein Thermostability Assay
Ilse Vandecaetsbeek and Peter Vangheluwe
Part II: Activity Assays
10. Colorimetric Assays of Na, K-ATPase
Kathleen J. Sweadner
11. ATPase Activity Measurements by an Enzyme-Coupled Spectrophotometric Assay Pankaj Sehgal, Claus Olesen, and Jesper V. M ller
12. Antimony-Phosphomolybdate ATPase Assay
Gianluca Bartolommei and Francesco Tadini-Buoninsegni
13. ATPase Activity Measurements using radiolabeled ATP
Herman G.P. Swarts and Jan B. Koenderink
14. Assaying P-type ATPases Reconstituted in Liposomes
Hans-Jьrgen Apell and Bojana Damnjanovic
15. Coupling ratio for Ca2+ Transport by Calcium Oxalate Precipitation
Pankaj Sehgal, Claus Olesen, and Jesper V. M ller
16. Calcium Uptake in Crude Tissue Preparation
Philip A. Bidwell and Evangelia G. Kranias
17. Measuring H+ Pumping and Membrane Potential Formation in Sealed Membrane Vesicle Systems
Alex Green Wielandt, Michael G. Palmgren, Anja Thoe Fuglsang, Thomas Gьnther Pomorski, and Bo H jen Justesen
18. Assay of Flippase Activity in Proteoliposomes using Fluorescent Lipid Derivatives Magdalena Marek and Thomas Gьnther Pomorski
Part III: In vitro Functional Studies
19. The use of Metal Fluoride Compounds as Phosphate Analogs for Understanding the Structural Mechanism in P-type ATPases
Stefania J. Danko and Hiroshi Suzuki
20. Phosphorylation/Dephosphorylation Assays
Hiroshi Suzuki
21. Tryptophan Fluorescence Changes Related to Ca2+-ATPase Function
Pankaj Sehgal, Claus Olesen, and Jesper V. M ller
Part IV: Ligand Binding Studies
22. Determination of the ATP Affinity of the Sarcoplasmic Reticulum Ca2+-ATPase by Competitive Inhibition of [g-32P]TNP-8N3-ATP Photolabeling
Johannes D. Clausen, David B. McIntosh, David G. Woolley, and Jens Peter Andersen
23. Ca2+ Binding and Transport Studied with Ca2+/EGTA Buffers and 45Ca2+
Pankaj Sehgal, Claus Olesen, and Jesper V. M ller
24. Assay of Copper Transfer and Binding to P1B-ATPases
Teresita Padilla-Benavides and Josй M. Argьello
Автор: Nathan Nelson Название: Organellar Proton-ATPases ISBN: 3662222671 ISBN-13(EAN): 9783662222676 Издательство: Springer Рейтинг: Цена: 11179.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Автор: Liselotte Plesner; Terence L. Kirley; Aileen F. Kn Название: Ecto-ATPases ISBN: 1461377293 ISBN-13(EAN): 9781461377290 Издательство: Springer Рейтинг: Цена: 6986.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Proceedings of the First International Workshop held in Mar de Plata, Argentina, August 26-30, 1996
Описание: This brief discusses the mechanism of functional expression of a protein or protein complex utilizing the ATP hydrolysis cycle or proton-motive force from a unique point of view focused on the roles of water. A variety of processes are considered such as the unidirectional movement of a linear-motor protein along a filament, insertion of an unfolded protein into a chaperonin and release of the folded protein from it, transport of diverse substrates across the membrane by a transporter, and directed rotation of the central subunit within a rotatory motor protein complex. These topics are discussed in a unified manner within the same theoretical framework. The author argues that water plays imperative roles in the functional expression of these molecular machines. A pivotal factor is the entropic force or potential originating from the translational displacement of water molecules coexisting with the molecular machines in the entire system.
Автор: Sajal Chakraborti; Naranjan S Dhalla Название: Regulation of Membrane Na+-K+ ATPase ISBN: 3319247484 ISBN-13(EAN): 9783319247489 Издательство: Springer Рейтинг: Цена: 27950.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Na+-K+ ATPase or Na-pump ATPase, a member of “P”-type ATPase superfamily, is characterized by association of multiple isoforms mainly of it’s ?- and ?- subunits. At present four different ?- (?-1,?-2,?-3 and ?-4) and three ?- (?-1, ?-2, and ?-3) isoforms have been identified in mammalian cells and their differential expressions are tissue specific. Regulation of Na+-K+ ATPase activity is an important but a complex process, which involves short-term and long-term mechanisms. Short-term regulation of Na+-K+ ATPase is either mediated by changes in intracellular Na+ concentrations that directly affect the Na+-pump activity or by phosphorylation/dephosphorylation-mediated by some stimulants leading to changes in its expression and transport properties. On the other hand, long-term regulation of Na+-K+ ATPase is mediated by hormones, such as mineralocorticoids and thyroid hormones, which cause changes in the transcription of genes of ?- and ?- subunits leading to an increased expression in the level of Na+-pump. Several studies have revealed a relatively new type of regulation that involves the association of small, single span membrane proteins with this enzyme. These proteins belong to the FXYD family, the members of which share a common signature sequence encompassing the transmembra ne domain adjacent to the isoform(s) of ?-? subunits of Na+-K+ ATPase. Considering the extraordinary importance of Na+-K+ ATPase in cellular function, several internationally established investigators have contributed their articles in the monograph entitled “Regulation of Membrane Na+-K+ ATPase” for inspiring young scientists and graduate students to enrich their knowledge on the enzyme, and we are sure that this book will soon be considered as a comprehensive scientific literature in the area of Na+-K+ ATPase regulation in health and disease.
Название: P-type atpases ISBN: 1493931784 ISBN-13(EAN): 9781493931781 Издательство: Springer Рейтинг: Цена: 27950.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
1. An Introduction to P-type ATPase research
Poul Nissen
Part I: Protein Production, Isolation, Purification, and Stabilization
2. Purification of Na, K-ATPase from Pig Kidney
Natalya U. Fedosova
3. Preparation of Ca2+-ATPase1a Enzyme from Rabbit Sarcoplasmic Reticulum
Jesper V. M ller and Claus Olesen
4. Isolation of H+/K+-ATPase-enriched Membrane Fraction from Pig Stomachs
Kazuhiro Abe and Claus Olesen
5. Overproduction of PiB-type ATPases
Xiangyu Liu, Oleg Sitsel, Kaituo Wang, and Pontus Gourdon
6. Coordinated Overexpression in Yeast of a P4-ATPase and its Associated Cdc50 Subunit: The Case of the Drs2p/Cdc50p Lipid Flippase Complex
Hassina Azouaoui, Cйdric Montigny, Aurore Jacquot, Raphaлlle Barry, Philippe Champeil, and Guillaume Lenoir
7. The Plasma Membrane Ca2+-ATPase: Purification by Calmodulin Affinity Chromatography, and Reconstitution of the Purified Protein
Verena Niggli and Ernesto Carafoli
8. Expression of Na, K-ATPase and H, K-ATPase Isoforms With The Baculovirus Expression System
Jan B. Koenderink and Herman G.P. Swarts
9. Time-dependent Protein Thermostability Assay
Ilse Vandecaetsbeek and Peter Vangheluwe
Part II: Activity Assays
10. Colorimetric Assays of Na, K-ATPase
Kathleen J. Sweadner
11. ATPase Activity Measurements by an Enzyme-Coupled Spectrophotometric Assay Pankaj Sehgal, Claus Olesen, and Jesper V. M ller
12. Antimony-Phosphomolybdate ATPase Assay
Gianluca Bartolommei and Francesco Tadini-Buoninsegni
13. ATPase Activity Measurements using radiolabeled ATP
Herman G.P. Swarts and Jan B. Koenderink
14. Assaying P-type ATPases Reconstituted in Liposomes
Hans-Jьrgen Apell and Bojana Damnjanovic
15. Coupling ratio for Ca2+ Transport by Calcium Oxalate Precipitation
Pankaj Sehgal, Claus Olesen, and Jesper V. M ller
16. Calcium Uptake in Crude Tissue Preparation
Philip A. Bidwell and Evangelia G. Kranias
17. Measuring H+ Pumping and Membrane Potential Formation in Sealed Membrane Vesicle Systems
Alex Green Wielandt, Michael G. Palmgren, Anja Thoe Fuglsang, Thomas Gьnther Pomorski, and Bo H jen Justesen
18. Assay of Flippase Activity in Proteoliposomes using Fluorescent Lipid Derivatives Magdalena Marek and Thomas Gьnther Pomorski
Part III: In vitro Functional Studies
19. The use of Metal Fluoride Compounds as Phosphate Analogs for Understanding the Structural Mechanism in P-type ATPases
Stefania J. Danko and Hiroshi Suzuki
20. Phosphorylation/Dephosphorylation Assays
Hiroshi Suzuki
21. Tryptophan Fluorescence Changes Related to Ca2+-ATPase Function
Pankaj Sehgal, Claus Olesen, and Jesper V. M ller
Part IV: Ligand Binding Studies
22. Determination of the ATP Affinity of the Sarcoplasmic Reticulum Ca2+-ATPase by Competitive Inhibition of [g-32P]TNP-8N3-ATP Photolabeling
Johannes D. Clausen, David B. McIntosh, David G. Woolley, and Jens Peter Andersen
23. Ca2+ Binding and Transport Studied with Ca2+/EGTA Buffers and 45Ca2+
Pankaj Sehgal, Claus Olesen, and Jesper V. M ller
24. Assay of Copper Transfer and Binding to P1B-ATPases
Teresita Padilla-Benavides and Josй M. Argьello
Автор: Sajal Chakraborti; Naranjan S Dhalla Название: Regulation of Ca2+-ATPases,V-ATPases and F-ATPases ISBN: 3319796771 ISBN-13(EAN): 9783319796772 Издательство: Springer Рейтинг: Цена: 27950.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: The biological membranes of cellular organization enfold an important group of membrane proteins called the ATPases, which are not only versatile in maintaining chemical gradient and electrical potential across the membrane but also bring metabolites necessary for cell metabolism and drive out toxins, waste products and solutes that otherwise can curb cell functions. ATPases are distributed virtually in all live forms starting from unicellular to multicellular and also in viruses. There are different types of ATPases, which differ in function and structure and in the type of ions they transport. The three main types of the ion pump ATPase family are: (i) P-type ATPases that transport different ions across membranes and Ca2+ATPases belongs to this catagory (ii) F-type ATPase in mitochondria, chloroplasts and bacterial plasma membranes produce ATP using the proton gradient; and (iii) V-type ATPase catalyzes ATP hydrolysis to transport solutes and maintains acidic pH in organelles like lysosomes. Genetic defects in either of the ATPases cause several diseases and a number of researches have demonstrated the involvement of the members of ATPases in the cell pathology and diseases, thereby penetrating exciting new areas of our understanding. In this book, the authors summarize recent knowledge about the molecular mechanisms associated with Ca2+-ATPase, V-ATPase and F-ATPase in intracellular and extracellular Ca2+ transport, mitochondrial ATP synthase, vesicular H+ transport, and lysosomal pH regulation. This book thereby bridges the gap between fundamental research and biomedical and pharmaceutical applications. The book provides an informative resource to improve ATPase research and modern therapeutic approaches toward different life threatening diseases that are associated with dysregulation of the ATPases.
Автор: Sajal Chakraborti; Naranjan S Dhalla Название: Regulation of Ca2+-ATPases,V-ATPases and F-ATPases ISBN: 3319247786 ISBN-13(EAN): 9783319247786 Издательство: Springer Рейтинг: Цена: 34937.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: The three main types of the ion pump ATPase family are: (i) P-type ATPases that transport different ions across membranes and Ca2+ATPases belongs to this catagory (ii) F-type ATPase in mitochondria, chloroplasts and bacterial plasma membranes produce ATP using the proton gradient;
Автор: Chakraborti Sajal, Dhalla Naranjan S. Название: Regulation of Membrane Na+-K+ Atpase ISBN: 3319796720 ISBN-13(EAN): 9783319796727 Издательство: Springer Рейтинг: Цена: 20962.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Na+-K+ ATPase or Na-pump ATPase, a member of “P”-type ATPase superfamily, is characterized by association of multiple isoforms mainly of it’s ?- and ?- subunits. At present four different ?- (?-1,?-2,?-3 and ?-4) and three ?- (?-1, ?-2, and ?-3) isoforms have been identified in mammalian cells and their differential expressions are tissue specific. Regulation of Na+-K+ ATPase activity is an important but a complex process, which involves short-term and long-term mechanisms. Short-term regulation of Na+-K+ ATPase is either mediated by changes in intracellular Na+ concentrations that directly affect the Na+-pump activity or by phosphorylation/dephosphorylation-mediated by some stimulants leading to changes in its expression and transport properties. On the other hand, long-term regulation of Na+-K+ ATPase is mediated by hormones, such as mineralocorticoids and thyroid hormones, which cause changes in the transcription of genes of ?- and ?- subunits leading to an increased expression in the level of Na+-pump. Several studies have revealed a relatively new type of regulation that involves the association of small, single span membrane proteins with this enzyme. These proteins belong to the FXYD family, the members of which share a common signature sequence encompassing the transmembra ne domain adjacent to the isoform(s) of ?-? subunits of Na+-K+ ATPase. Considering the extraordinary importance of Na+-K+ ATPase in cellular function, several internationally established investigators have contributed their articles in the monograph entitled “Regulation of Membrane Na+-K+ ATPase” for inspiring young scientists and graduate students to enrich their knowledge on the enzyme, and we are sure that this book will soon be considered as a comprehensive scientific literature in the area of Na+-K+ ATPase regulation in health and disease.
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