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RNA Detection and Visualization: Methods and Protocols, Gerst Jeffrey E.


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Цена: 20962.00р.
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Автор: Gerst Jeffrey E.
Название:  RNA Detection and Visualization: Methods and Protocols
ISBN: 9781493958368
Издательство: Springer
Классификация:

ISBN-10: 1493958364
Обложка/Формат: Paperback
Страницы: 497
Вес: 0.88 кг.
Дата издания: 23.08.2016
Серия: Methods in molecular biology
Язык: English
Издание: Softcover reprint of
Иллюстрации: 7 tables, black and white; xiv, 497 p.
Размер: 25.40 x 17.78 x 2.62 cm
Читательская аудитория: General (us: trade)
Подзаголовок: Methods and protocols
Ссылка на Издательство: Link
Рейтинг:
Поставляется из: Германии
Описание: With its complex and extensively regulated metabolism, the study of the RNA lifecycle demands tools that allow for the localization of RNAs to be observed either in an in situ setting or, preferably, under in vivo conditions. In RNA Detection and Visualization: Methods and Protocols, the best and brightest investigators provide an up-to-date and in-depth description of basic methods and protocols used for detecting and visualizing mRNAs in both fixed and live cells, from bacteria to mammals. For novices and experts alike, this mix of classic in situ hybridization and advanced live imaging techniques, cell fractionation and affinity purification procedures, and bioinformatics tools gives researchers the most complete and extensive array of research aids possible. As a volume written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, RNA Detection and Visualization: Methods and Protocols offers well-honed techniques in order to inspire researchers around the world to further our knowledge of the vital biological significance of RNA.
Дополнительное описание: Single Molecule Imaging of RNA In situ.- FISH and Immunofluorescence Staining in Chlamydomonas.- High Resolution Fluorescent In situ Hybridization in Drosophila.- Localization and Anchorage of Maternal mRNAs to Cortical Structures of Ascidian Eggs and Emb



In Situ Detection of DNA Damage / Methods and Protocols

Автор: Didenko Vladimir V.
Название: In Situ Detection of DNA Damage / Methods and Protocols
ISBN: 0896039528 ISBN-13(EAN): 9780896039520
Издательство: Springer
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Цена: 5809.00 р.
Наличие на складе: Есть (4 шт.)
Описание: In In Situ Detection of DNA Damage: Methods and Protocols, Vladimir Didenko and a panel of experts describe all the major in situ techniques for studying DNA damage and apoptosis, and how approaches originally designed to label apoptotic cells can be used for DNA damage analysis (and vice versa). The readily reproducible techniques presented here include all those designed to detect specific single- and double-stranded DNA breaks in tissue sections while using polymerases, ligases, nucleases, and kinases. There are also novel instrumental techniques that use ultrasound for in vivo and in situ detection of apoptotic DNA damage, and in situ applications of flow and laser-scanning cytometry for analysis of DNA strand breaks and apoptosis. State-of-the-art and highly practical, In Situ Detection of DNA Damage: Methods and Protocols presents for the first time a complete set of enzymatic methods for determining DNA breaks, and will greatly facilitate the work of all those studying the in situ detection of DNA damage and the labeling of apoptotic cells.

Affinity Chromatography / Methods and Protocols

Автор: Zachariou Michael
Название: Affinity Chromatography / Methods and Protocols
ISBN: 1588296598 ISBN-13(EAN): 9781588296597
Издательство: Springer
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Цена: 2000.00 р.
Наличие на складе: Есть (2 шт.)
Описание: Thirty-eight years after its introduction, affinity chromatography remains a key tool in th armory of separation techniques available to separation and interaction scientists.  Expanded and updated from the first edition, this second edition volume aims to provide the beginner with the practical knowledge needed to develop affinity separations suitable for a variety of applications relevant to the post-genomic era.

Immunocytochemical methods and protocols

Автор: Constance Oliver; Maria Celia Jamur (Eds.)
Название: Immunocytochemical methods and protocols
ISBN: 1588294633 ISBN-13(EAN): 9781588294630
Издательство: Springer
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Цена: 9084.00 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание: This third edition reflects the rapid evolution of immunocytochemistry in the post-genomic era. Expert researchers explore the latest methods, now widely used in situ to identify components of cells and tissues in both normal and pathological conditions.

HPLC of Peptides and Proteins / Methods and Protocols

Автор: Aguilar Marie-Isabel
Название: HPLC of Peptides and Proteins / Methods and Protocols
ISBN: 0896039773 ISBN-13(EAN): 9780896039773
Издательство: Springer
Рейтинг:
Цена: 23757.00 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание: Hands-on experts from academia and industry comprehensively describe how to successfully perform all the critical HPLC techniques needed for the analysis of peptides and proteins. The methods range from commonly used techniques to those for capillary to large-scale preparative isolation. The authors have also presented a number of specific applications as case studies to illustrate the analytical approaches to a particular separation or assay challenge, with examples drawn from contemporary fields in biochemistry and biotechnology.


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